Un’anemia emolitica da sferocitosi ereditaria

2021 ◽  
Vol 40 (3) ◽  
pp. 194-195
Author(s):  
Davide Ursi ◽  
Maria Sole Valentino
Keyword(s):  
Haemolytic Anaemia ◽  
False Positive ◽  
Resistance Test ◽  
Osmotic Resistance ◽  
Blood Tests ◽  
Positive Results

A case of haemolytic anaemia due to spherocytosis in a 3-month-old infant is reported. It is important to remember that post-transfusion blood tests are not reliable and must be repeated 3 months after the transfusion. The erythrocyte osmotic resistance test can give false positive results in the first months of life and must be repeated in the sixth month.

Diagnosis Of Deep Venous Thrombosis By Combined Non-Invasive Flow Studies And Coagulation Tests

1981 ◽  
Author(s):  
J A Caprini ◽  
D R Watts ◽  
C A Franck ◽  
A R Crampton ◽  
J P Vagher ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Venous Thrombosis ◽  
Deep Venous Thrombosis ◽  
False Positive ◽  
Impedance Plethysmography ◽  
Non Invasive ◽  
Blood Tests ◽  
Coagulation Tests ◽  
The Face ◽  
Positive Results

The diagnostic accuracy of Doppler ultrasound, impedance plethysmography, activated thrombelastography (TEG), and fibrin split products (FSP) assays were compared to the results of ascending venography in 127 consecutive patients with suspected deep venous thrombosis (DVT). The combination of Doppler with impedance plethysmography was 96% accurate in predicting normal limbs and 83% accurate in identifying limbs harboring thrombi. This combination of flow tests was more sensitive in detecting clots at the popliteal level or above (15/17 88%) than in the calf veins (18/23 78%). The coagulation tests had a high percentage of false positive results (TEG - 39%; FSP - 52%). TEG and FSP combined were 79% (67/87) accurate in normal limbs; 72% (29/40) accurate in those with thrombosis. 0/87 patients had all four tests positive in the face of a normal venogram. 1/40 patients with thrombosis had normal results with all four tests. This patient only had calf clots.These results suggest that the addition of blood tests to flow studies does improve sensitivity and specificity when all tests are either positive or negative. The presence of normal blood tests and flow studies completely excluded thrombi in this series.

Critical Evaluation of Impedance Phlebography for the Diagnosis of Deep Vein Thrombosis

1974 ◽  
Vol 31 (02) ◽  
pp. 273-278
Author(s):  
Kenneth K Wu ◽  
John C Hoak ◽  
Robert W Barnes ◽  
Stuart L Frankel
Keyword(s):  
Deep Vein Thrombosis ◽  
False Positive ◽  
False Negative ◽  
Critical Evaluation ◽  
Original Method ◽  
Vein Thrombosis ◽  
Negative Results ◽  
False Negative Results ◽  
Deep Vein ◽  
Positive Results

SummaryIn order to evaluate its daily variability and reliability, impedance phlebography was performed daily or on alternate days on 61 patients with deep vein thrombosis, of whom 47 also had 125I-fibrinogen uptake tests and 22 had radiographic venography. The results showed that impedance phlebography was highly variable and poorly reliable. False positive results were noted in 8 limbs (18%) and false negative results in 3 limbs (7%). Despite its being simple, rapid and noninvasive, its clinical usefulness is doubtful when performed according to the original method.

Comparison of two rapid tests for the detection of legionellaceae in water: a microbiological-immunological method and a commercial gene-probe testkit

1995 ◽  
Vol 31 (5-6) ◽  
pp. 403-406 ◽  
Author(s):  
E. Frahm ◽  
U. Obst
Keyword(s):  
Monoclonal Antibodies ◽  
Conventional Method ◽  
Standard Method ◽  
False Positive ◽  
Gene Probe ◽  
Immunological Method ◽  
Probe Test ◽  
Rapid Tests ◽  
Positive Results

Two recently developed Legionella detection tests, a microbiological-immunological method based on monoclonal antibodies (carried out as a colony-blot assay) and a commercial gene-probe testkit (the EnvironAmp Legionella Kit), are compared with the standard method. The colony-blot assay is faster than the conventional method; the gene-probe test is much faster still and is the most sensitive, but in consequence is at greater risk of false-positive results.

scholarly journals The Effect of Genomic DNA Contamination on the Detection of Circulating Long Non-Coding RNAs: The Paradigm of MALAT1

Diagnostics ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1160
Author(s):  
Athina N. Markou ◽  
Stavroula Smilkou ◽  
Emilia Tsaroucha ◽  
Evi Lianidou
Keyword(s):  
False Positive ◽  
Tissue Samples ◽  
Dna Contamination ◽  
Dnase Treatment ◽  
Circular Dnas ◽  
Non Coding Rnas ◽  
Nsclc Patients ◽  
Treatment Step ◽  
Primary Tissue ◽  
Positive Results

The presence of contaminating gDNA in RNA preparations is a frequent cause of false positives in RT-PCR-based analysis. However, in some cases, this cannot be avoided, especially when there are no exons–intron junctions in the lncRNA sequences. Due to the lack of exons in few of long noncoding RNAs (lncRNAs) and the lack of DNAse treatment step in most studies reported so far, serious questions are raised about the specificity of lncRNA detection and the potential of reporting false-positive results. We hypothesized that minute amounts of gDNA usually co-extracted with RNA could give false-positive signals since primers would specifically bind to gDNA due to the lack of junction. In the current study, we evaluated the effect of gDNA and other forms of DNA like extrachromosomal circular DNAs (eccDNAs) contamination and the importance of including a DNAse treatment step on lncRNAsexpression.As a model, we have chosen as one of the most widely studied lncRNAs in cancer namely MALAT1, which lacks exons. When we tested this hypothesis in plasma and primary tissue samples from NSCLC patients, our findings clearly indicated that results on MALAT1 expression are highly affected by the presence of DNA contamination and that the DNAse treatment step is absolutely necessary to avoid false positive results.

Clinical features and cause analysis of false positive results of Aspergillus galactomannan assay in pulmonary cryptococcosis patients

2019 ◽  
Vol 38 (4) ◽  
pp. 735-741 ◽  
Author(s):  
Takahiro Takazono ◽  
Tomomi Saijo ◽  
Nobuyuki Ashizawa ◽  
Kazuhiro Oshima ◽  
Keitaro Nishimura ◽  
...  
Keyword(s):  
Clinical Features ◽  
False Positive ◽  
Pulmonary Cryptococcosis ◽  
Cause Analysis ◽  
Positive Results
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