Tumor Necrosis Factor-α and Interleukin-1β Treatment of Endometrial Stromal Cells Does not Promote Their Adhesion to Peritoneal Mesothelial Cells in an in Vitro Model of Early-Stage Endometriosis

2011 ◽  
Vol 3 (2) ◽  
pp. 67-72
Author(s):  
Jean-Christophe Lousse ◽  
Sylvie Defrere ◽  
Sébastien Colette ◽  
Anne Van Langendonckt ◽  
Jacques Donnez
Keyword(s):  
Cell Adhesion ◽  
In Vitro Model ◽  
Early Stage ◽  
Mesothelial Cells ◽  
Endometrial Stromal Cells ◽  
Peritoneal Mesothelial Cells ◽  
Pre Treatment ◽  
Vitro Model ◽  
The Impact

In this study, we developed an original and reproducible quantitative in vitro model of endometrial cell adhesion to peritoneal mesothelial cells in order to better assess the impact of pro-inflammatory cytokines on early-stage endometriosis development. We demonstrated that pre-treatment with TNF-α and IL-1β does not promote endometrial stromal cell adhesion to peritoneal mesothelial cells.

Co-cultured endometrial stromal cells and peritoneal mesothelial cells for an in vitro model of endometriosis

2012 ◽  
Vol 4 (9) ◽  
pp. 1090 ◽  
Author(s):  
Zhenling Chen ◽  
Yi Dai ◽  
Zhe Dong ◽  
Menghui Li ◽  
Xuan Mu ◽  
...  
Keyword(s):  
Stromal Cells ◽  
In Vitro Model ◽  
Mesothelial Cells ◽  
Endometrial Stromal Cells ◽  
Peritoneal Mesothelial Cells ◽  
Vitro Model

Mesothelial Cells

2007 ◽  
Vol 27 (2_suppl) ◽  
pp. 110-115 ◽  
Author(s):  
Susan Yung ◽  
Chan Tak Mao
Keyword(s):  
Mesothelial Cell ◽  
In Vitro Models ◽  
Mesothelial Cells ◽  
Dynamic Membrane ◽  
Peritoneal Mesothelial Cells ◽  
Immunohistochemical Markers ◽  
Vitro Model ◽  
And Function

♦ Background The introduction of peritoneal dialysis (PD) as a modality of renal replacement therapy has provoked much interest in the biology of the peritoneal mesothelial cell. Mesothelial cells isolated from omental tissue have immunohistochemical markers that are identical to those of mesothelial stem cells, and omental mesothelial cells can be cultivated in vitro to study changes to their biologic functions in the setting of PD. ♦ Method The present article describes the structure and function of mesothelial cells in the normal peritoneum and details the morphologic changes that occur after the introduction of PD. Furthermore, this article reviews the literature of mesothelial cell culture and the limitations of in vitro studies. ♦ Results The mesothelium is now considered to be a dynamic membrane that plays a pivotal role in the homeostasis of the peritoneal cavity, contributing to the control of fluid and solute transport, inflammation, and wound healing. These functional properties of the mesothelium are compromised in the setting of PD. Cultures of peritoneal mesothelial cells from omental tissue provide a relevant in vitro model that allows researchers to assess specific molecular pathways of disease in a distinct population of cells. Structural and functional attributes of mesothelial cells are discussed in relation to long-term culture, proliferation potential, age of tissue donor, use of human or animal in vitro models, and how the foregoing factors may influence in vitro data. ♦ Conclusions The ability to propagate mesothelial cells in culture has resulted, over the past two decades, in an explosion of mesothelial cell research pertaining to PD and peritoneal disorders. Independent researchers have highlighted the potential use of mesothelial cells as targets for gene therapy or transplantation in the search to provide therapeutic strategies for the preservation of the mesothelium during chemical or bacterial injury.

scholarly journals CAFs affect the proliferation and treatment response of head and neck cancer spheroids during co-culturing in a unique in vitro model

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Mustafa Magan ◽  
Emilia Wiechec ◽  
Karin Roberg
Keyword(s):  
Cell Proliferation ◽  
Tumor Cell ◽  
Head And Neck ◽  
Treatment Response ◽  
Tumor Cells ◽  
In Vitro Model ◽  
Tumor Spheroids ◽  
Vitro Model ◽  
The Impact

Abstract Background Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous group of tumors for which the overall survival rate worldwide is around 60%. The tumor microenvironment, including cancer-associated fibroblasts (CAFs), is believed to affect the treatment response and migration of HNSCC. The aim of this study was to create a biologically relevant HNSCC in vitro model consisting of both tumor cells and CAFs cultured in 3D to establish predictive biomarkers for treatment response, as well as to investigate the impact of CAFs on phenotype, proliferation and treatment response in HNSCC cells. Methods Three different HNSCC patient-derived tumor cell lines were cultured with and without CAFs in a 3D model. Immunohistochemistry of the proliferation marker Ki67, epidermal growth factor receptor (EGFR) and fibronectin and a TUNEL-assay were performed to analyze the effect of CAFs on both tumor cell proliferation and response to cisplatin and cetuximab treatment in tumor spheroids (3D). mRNA expression of epithelial-mesenchymal transition (EMT) and cancer stem cells markers were analyzed using qRT-PCR. Results The results demonstrated increased cell proliferation within the tumor spheroids in the presence of CAFs, correlating with increased expression of EGFR. In spheroids with increased expression of EGFR, a potentiated response to cetuximab treatment was observed. Surprisingly, an increase in Ki67 expressing tumor cells were observed in spheroids treated with cisplatin for 3 days, correlating with increased expression of EGFR. Furthermore, tumor cells co-cultured with CAFs presented an increased EMT phenotype compared to tumor cells cultured alone in 3D. Conclusion Taken together, our results reveal increased cell proliferation and elevated expression of EGFR in HNSCC tumor spheroids in the presence of CAFs. These results, together with the altered EMT phenotype, may influence the response to cetuximab or cisplatin treatment.

Development of an in vitro model of the early-stage bovine tuberculous granuloma using Mycobacterium bovis-BCG

2015 ◽  
Vol 168 (3-4) ◽  
pp. 249-257 ◽  
Author(s):  
Laura Garza-Cuartero ◽  
Elaine McCarthy ◽  
Joseph Brady ◽  
Joseph Cassidy ◽  
Clare Hamilton ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
In Vitro Model ◽  
Early Stage ◽  
Mycobacterium Bovis Bcg ◽  
Tuberculous Granuloma ◽  
Vitro Model

Influence of Bacillus subtilis C-3102 on microbiota in a dynamic in vitro model of the gastrointestinal tract simulating human conditions

2012 ◽  
Vol 3 (3) ◽  
pp. 229-236 ◽  
Author(s):  
M. Hatanaka ◽  
Y. Nakamura ◽  
A.J.H. Maathuis ◽  
K. Venema ◽  
I. Murota ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Gastrointestinal Tract ◽  
In Vitro Model ◽  
Germination Rate ◽  
Human Colon ◽  
Micro Array ◽  
Vitro Model ◽  
Oral Doses ◽  
The Impact

Survival and germination rate of Bacillus subtilis C-3102 spores were investigated in a stomach and small intestine model (TIM-1), while the impact of C-3102 cells that had passed through TIM-1 on human colon microbiota was evaluated in a model of the large intestine (TIM-2). The survival of C-3102 spores in TIM-1 was 99%; 8% of the spores had germinated. Effluent of TIM-1 was subsequently introduced into TIM-2 and a micro-array platform was employed to assess changes in the microbiota composition. The effluent, which contained germinated C-3102 cells, increased some Bifidobacterium species and decreased some Clostridium groups. These changes were greater compared to those obtained by adding C-3102 spores directly to TIM-2. The present study suggests that oral doses of B. subtilis C-3102 spores have the potential to modulate the human colon microbiota. This effect may be caused by germination of the spores in the gastrointestinal tract.

Hormonal regulation of human endometrial stromal cells in culture: an in vitro model for decidualization

1989 ◽  
Vol 52 (5) ◽  
pp. 761-768 ◽  
Author(s):  
Juan C. Irwin ◽  
David Kirk ◽  
Roger J.B. King ◽  
Martin M. Quigley ◽  
Ralph B.L. Gwatkin
Keyword(s):  
Stromal Cells ◽  
Hormonal Regulation ◽  
In Vitro Model ◽  
Endometrial Stromal Cells ◽  
Cells In Culture ◽  
Vitro Model

scholarly journals Hypoxia induces stress fiber formation in adipocytes in the early stage of obesity

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Golnaz Anvari ◽  
Evangelia Bellas
Keyword(s):  
In Vitro Model ◽  
Nuclear Translocation ◽  
Early Stage ◽  
Coiled Coil ◽  
Stress Fiber ◽  
Fiber Formation ◽  
Actin Stress Fiber ◽  
Stress Fiber Formation ◽  
Vitro Model

AbstractIn obese adipose tissue (AT), hypertrophic expansion of adipocytes is not matched by new vessel formation, leading to AT hypoxia. As a result, hypoxia inducible factor-1⍺ (HIF-1⍺) accumulates in adipocytes inducing a transcriptional program that upregulates profibrotic genes and biosynthetic enzymes such as lysyl oxidase (LOX) synthesis. This excess synthesis and crosslinking of extracellular matrix (ECM) components cause AT fibrosis. Although fibrosis is a hallmark of obese AT, the role of fibroblasts, cells known to regulate fibrosis in other fibrosis-prone tissues, is not well studied. Here we have developed an in vitro model of AT to study adipocyte-fibroblast crosstalk in a hypoxic environment. Further, this in vitro model was used to investigate the effect of hypoxia on adipocyte mechanical properties via ras homolog gene family member A (RhoA)/Rho-associated coiled-coil kinases (ROCK) signaling pathways. We confirmed that hypoxia creates a diseased phenotype by inhibiting adipocyte maturation and inducing actin stress fiber formation facilitated by myocardin-related transcription factor A (MRTF-A/MKL1) nuclear translocation. This work presents new potential therapeutic targets for obesity by improving adipocyte maturation and limiting mechanical stress in obese AT.

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