Investigation of BK virus, Epstein-Barr virus and human papillomavirus sequences in postoperative thyroid gland specimens

2015 ◽  
Vol 30 (1) ◽  
pp. 104-110 ◽  
Author(s):  
Dimitris Stamatiou ◽  
Stavros P. Derdas ◽  
Emmanouil K. Symvoulakis ◽  
Georgios H. Sakorafas ◽  
Odysseas Zoras ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Epstein Barr Virus ◽  
Thyroid Tissue ◽  
Bk Virus ◽  
Gene Sequences ◽  
Normal Thyroid Tissue ◽  
Normal Thyroid ◽  
Barr Virus ◽  
Epstein Barr ◽  
Tissue Specimens

Background Although recent evidence has implicated viruses in the regulation of epithelial-to-mesenchymal transition and tumor progression, little is known regarding viral infections in thyroid malignancies. Thus the aim of this study was to detect sequences of 3 potentially oncogenic viruses – BK virus (BKV), Epstein-Barr virus (EBV) and human papillomavirus (HPV) – in a series of postoperative thyroid gland specimens. Methods Thirty patients with thyroid nodules who underwent surgery for thyroid disease within a 3-year period were enrolled. Both nodular and adjacent normal thyroid tissue was surgically excised from each patient. Viral gene sequences of BKV (VP1), EBV (LMP1, EBNA2 and EBER1) and HPV were amplified by PCR. The PCR results were confirmed by direct sequencing analysis. Results VP1 gene sequences were detected in 60% (18/30) of thyroid cancer or multinodular hyperplasia lesions compared with in 43.3% (13/30) of adjacent normal thyroid tissue specimens. Fifteen of thirty (50%) of thyroid cancer or multinodular hyperplasia samples revealed LMP1 sequences compared with 46.7% (14/30) of corresponding normal thyroid tissues. EBNA2 gene sequences were detected in 90% (27/30) of thyroid cancer or multinodular hyperplasia samples, compared with 90% (27/30) of adjacent normal thyroid tissue specimens. All samples were negative for EBER1 sequences, while HPV DNA was not detected in either nodular or normal thyroid tissue. Conclusions This study suggests that BKV and EBV “infection” is an early event, occurring within normal tissue. Our findings do not show a clear role for the viruses examined, instead they suggest an “endemicity” pattern rather than a causal effect.

scholarly journals Steroid receptor coactivator-1 interacts with NF-κB to increase VEGFC levels in human thyroid cancer

2018 ◽  
Vol 38 (3) ◽  
Author(s):  
Bo Gao ◽  
Lingji Guo ◽  
Donglin Luo ◽  
Yan Jiang ◽  
Jianjie Zhao ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Thyroid Tissue ◽  
Lymphatic Vessels ◽  
Steroid Receptor ◽  
Human Thyroid ◽  
Normal Thyroid Tissue ◽  
Normal Thyroid ◽  
Steroid Receptor Coactivator ◽  
Lymphatic Metastases ◽  
Factor C

Thyroid cancer is the most common endocrine cancer, and has a high incidence of lymphatic metastasis. Vascular endothelial growth factor C (VEGFC) is essential for development of lymphatic vessels and lymphatic metastases during carcinogenesis. Steroid receptor coactivator-1 (SRC-1) interacts with nuclear receptors and transcription factors to promote tumor proliferation and metastasis. However, the correlation between SRC-1 and VEGFC levels in the lymphatic metastases of thyroid cancer remains unclear. We analyzed 20-paired specimens of thyroid cancer tissue and normal thyroid tissue and found increased levels of SRC-1 and VEGFC proteins in 13/20 and 15/20 thyroid cancer specimens, respectively, when compared with those levels in specimens of normal thyroid tissue. A high level of SRC-1 expression was positively correlated with VEGFC and lymphatic endothelial cell marker LYVE-1 expression. Papillary thyroid carcinoma cell line TPC-1 displayed high levels of SRC-1 and VEGFC expression and was selected for stable knockdown of SRC-1 in vitro. Inhibition of SRC-1 significantly reduced the VEGFC levels in TPC-1 cells. We found that SRC-1 binds to transcription factor NF-kB (p50/p65), and that this coactivation complex directly promoted VEGFC transcription, which could be abrogated by SRC-1 knockdown. Up-regulated NF-kB signaling was also confirmed in thyroid cancer tissues. In vivo studies showed that SRC-1 knockdown restricted tumor growth, reduced the numbers of LYVE-1-positive lymphatic vessels, and decreased the levels of VEGFC in tumor tissues. These results suggest a tumorigenic role for SRC-1 in thyroid cancer via its ability to regulate VEGFC expression.

scholarly journals Prevalence of Polyoma BK Virus (BKPyV) , Epstein-Barr Virus (EBV) and Human Papilloma Virus (HPV) in Oropharyngeal Cancer

2015 ◽  
Vol 64 (4) ◽  
pp. 323-328 ◽  
Author(s):  
Dorota Polz-Gruszka ◽  
Kamal Morshed ◽  
Adrian Jarzyński ◽  
Małgorzata Polz-Dacewicz
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Oropharyngeal Cancer ◽  
Epstein Barr Virus ◽  
Bk Virus ◽  
Papilloma Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Pre Treatment ◽  
Paraffin Tissue

The aim of this study was to analyze the prevalence of BK virus, Human Papillomavirus and Epstein-Barr virus in oropharyngeal cancer, and to test our hypothesis that BKV/HPV/EBV co-infection plays a role in oropharyngeal squamous cell carcinoma. The correlation between viral infection, OSCC, anatomic location, pre-treatment staging, evidence of metastases to lymph nodes, and grading was also investigated. The examination samples were collected from 62 patients from paraffin tissue blocks. Males (90.3%) with, smoking (83.9%) and alcohol abuse (67.7%) problems prevailed in the studied group. G2 histological type was recognized in 80.6% cases. T4 (77.4%) and N2 (56.5%) traits occurred in the majority of patients. No cases of metastasis were observed (M0 100%). HPV – 24.2%, EBV – 27.4% and BKV 17.7% were detected in the studied samples. We observed co-infection EBV/BKV in 8% of cases, HPV/BKV in 4.8%, and HPV/EBV in 9% cases. Only in two cases co-infection of all three viruses was found.

scholarly journals Epstein-Barr virus, human cytomegalovirus, human herpesvirus 6 and 7, human adenovirus, John Cunningham virus, and BK virus are not associated with gliomas in humans

2021 ◽  
Author(s):  
Bożena Czarkowska-Pączek ◽  
Rafal Górski ◽  
Aleksandra Wyczałkowska-Tomasik ◽  
Maciej Bujko ◽  
Leszek Pączek
Keyword(s):  
Epstein Barr Virus ◽  
Human Herpesvirus ◽  
Human Adenovirus ◽  
Bk Virus ◽  
John Cunningham Virus ◽  
Barr Virus ◽  
Risk Of Death ◽  
Course Material ◽  
Epstein Barr

Introduction: The literature points to several viruses associated with brain carcinogenesis, including gliomas. Aim: The aim of this study was to assess the presence of several viruses in gliomas and plasma from patients with brain tumor and the possible association of viral positivity with the clinical course. Material and methods: The study group consisted of 37 patients with gliomas who were subjected to surgery. Mean patient age was 54.59 (SD 15.85) years. The presence of viral DNA was assessed using real-time polymerase chain reaction. Results and discussion: We did not confirm any BK virus, John Cunningham virus, or human adenovirus-positive gliomas. The percentage of patients with gliomas positive for Epstein-Barr virus (EBV), cytomegalovirus (CMV), and human herpesvirus (HHV) 6 and 7 was 18.9%, 8.1%, 2.7%, and 10.8%, respectively. We did not confirm the co-occurrence of glioma and plasma viral positivity. Fisher’s test did not reveal the influence of viral infection on the risk of death. We did not detect any differences regarding sex, WHO classification of gliomas, or the functional impairment evaluated by the Karnofsky scale index at admission to the hospital in the group of patients positive or negative for EBV, CMV, or HHV6. Conclusions: We cannot confirm an association of the investigated viruses with gliomas.

scholarly journals Off-the-Shelf Virus-Specific T Cells to Treat BK Virus, Human Herpesvirus 6, Cytomegalovirus, Epstein-Barr Virus, and Adenovirus Infections After Allogeneic Hematopoietic Stem-Cell Transplantation

2017 ◽  
Vol 35 (31) ◽  
pp. 3547-3557 ◽  
Author(s):  
Ifigeneia Tzannou ◽  
Anastasia Papadopoulou ◽  
Swati Naik ◽  
Kathryn Leung ◽  
Caridad A. Martinez ◽  
...  
Keyword(s):  
T Cells ◽  
Stem Cell ◽  
Epstein Barr Virus ◽  
Human Herpesvirus ◽  
Bk Virus ◽  
Third Party ◽  
Human Herpesvirus 6 ◽  
Hematopoietic Stem ◽  
Barr Virus ◽  
Epstein Barr

Purpose Improvement of cure rates for patients treated with allogeneic hematopoietic stem-cell transplantation (HSCT) will require efforts to decrease treatment-related mortality from severe viral infections. Adoptively transferred virus-specific T cells (VSTs) generated from eligible, third-party donors could provide broad antiviral protection to recipients of HSCT as an immediately available off-the-shelf product. Patient and Methods We generated a bank of VSTs that recognized five common viral pathogens: Epstein-Barr virus (EBV), adenovirus (AdV), cytomegalovirus (CMV), BK virus (BKV), and human herpesvirus 6 (HHV-6). The VSTs were administered to 38 patients with 45 infections in a phase II clinical trial. Results A single infusion produced a cumulative complete or partial response rate of 92% (95% CI, 78.1% to 98.3%) overall and the following rates by virus: 100% for BKV (n = 16), 94% for CMV (n = 17), 71% for AdV (n = 7), 100% for EBV (n = 2), and 67% for HHV-6 (n = 3). Clinical benefit was achieved in 31 patients treated for one infection and in seven patients treated for multiple coincident infections. Thirteen of 14 patients treated for BKV-associated hemorrhagic cystitis experienced complete resolution of gross hematuria by week 6. Infusions were safe, and only two occurrences of de novo graft-versus host disease (grade 1) were observed. VST tracking by epitope profiling revealed persistence of functional VSTs of third-party origin for up to 12 weeks. Conclusion The use of banked VSTs is a feasible, safe, and effective approach to treat severe and drug-refractory infections after HSCT, including infections from two viruses (BKV and HHV-6) that had never been targeted previously with an off-the-shelf product. Furthermore, the multispecificity of the VSTs ensures extensive antiviral coverage, which facilitates the treatment of patients with multiple infections.

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