Glycoprotein electrophoretic patterns have potential to monitor changes associated with neoplastic transformation in oral cancer

2012 ◽  
Vol 27 (3) ◽  
pp. 247-256 ◽  
Author(s):  
Bhairavi N. Vajaria ◽  
Kinjal R. Patel ◽  
Rasheedunnisa Begum ◽  
Jayendra B. Patel ◽  
Franky D. Shah ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Polyacrylamide Gel Electrophoresis ◽  
Neoplastic Transformation ◽  
Tobacco Consumption ◽  
Electrophoretic Patterns ◽  
Increasing Trend ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
Oral Cancer Patients ◽  
Oral Precancerous Conditions

Alterations in glycoproteins, important cell surface constituents, have long been associated with various malignancies. The present investigation therefore explored the clinical significance of a glycoproteomics approach in patients with oral precancerous conditions (OPC) and patients with oral cancer. The study included 80 oral cancer patients, 50 patients with OPC, and 84 controls. Native polyacrylamide gel electrophoresis followed by Schiff's staining was carried out to study the alterations in glycoproteins. The results showed significant elevation (p<0.0001) of 192 kDa, 170 kDa, 116 kDa and 44 kDa glycoproteins in oral cancer patients and patients with OPC compared with controls. The odds ratio indicated a significantly higher risk for oral cancer among users and especially chewers of tobacco. The levels of all the glycoprotein bands (192 kDa, 170 kDa, 116 kDa and 44 kDa) were higher in patients with a habit of tobacco use (WHT) than in patients with no habit of tobacco (NHT) and were also higher in WHT controls than in NHT controls. Moreover, a 230 kDa glycoprotein consistently appeared only in individuals with tobacco habits and an increasing trend was observed from WHT controls to patients with OPC to WHT oral cancer patients. In conclusion, the results indicated the potential utility of glycoprotein alterations in monitoring sequential changes occurring due to tobacco consumption during neoplastic transformation.

scholarly journals Correlation between Loss of E-cadherin, Matrix-metalloproteinases and c-Jun expression in Oral Carcinogenesis

2017 ◽  
Vol 3 (1) ◽  
pp. 1-24
Author(s):  
Bhairavi N. Vajaria ◽  
Kinjal R. Patel ◽  
Rasheedunnisa Begum ◽  
Franky D. Shah ◽  
Jayendra B. Patel ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Gelatin Zymography ◽  
Mrna Levels ◽  
Normal Tissues ◽  
Oral Carcinogenesis ◽  
Oral Cancer Patients ◽  
Mrna And Protein Expression ◽  
Oral Precancerous Conditions ◽  
E Cadherin

Background: Earlier invitro studies have observed that loss of E-cadherin is responsible for progression and metastasis of cancer by upregulation of c-Jun protein. There being lack of simultaneous evaluation of matrix metalloproteinase (MMP) -2 and -9, E-cadherin and c-Jun mRNA and protein. Objective: This study aimed to correlate the above-mentioned parameters to evaluate the pathway of oral carcinogenesis. Methods: The study included 100 controls, 50 patients with oral precancerous conditions (OPC) and 100 oral cancer patients. MMPs were evaluated by gelatin zymography, ECAD and CJUN mRNA and protein expression by semi quantitative RT-PCR and western blot respectively. Results: The levels of active and total MMP-2 and MMP-9 were significantly higher in patients with OPC and oral cancer patients as compared to controls. A significant increase in truncated E-cadherin and c-Jun protein was observed in malignant tissues as compared to adjacent normal tissues while CJUN mRNA levels were comparable. Higher values of c-Jun protein and MMPs, and lower values of ECAD mRNA were associated with reduced overall survival. A positive correlation was observed between truncated E-cadherin, MMPs and c-Jun protein. Conclusions: MMPs modulate cell-cell adhesion by increasing truncation of E-cadherin resulting in loss of E-cadherin which is responsible for upregulation of c-Jun protein in oral cancer.

scholarly journals A clinico-epidemiological hospital based study of oral cancer patients in Gwalior district

2021 ◽  
Vol 8 (2) ◽  
pp. 132-138
Author(s):  
Ankita Tripathi ◽  
Anil Kumar Agarwal ◽  
Ramniwas Mahore ◽  
Sumit S Bhadoriya ◽  
Swati Saraswat
Keyword(s):  
Oral Cancer ◽  
Oral Cavity ◽  
Cancer Patients ◽  
Indian Subcontinent ◽  
Tobacco Consumption ◽  
Cross Sectional Study ◽  
Age Group ◽  
Cross Sectional ◽  
Common Cancer ◽  
Oral Cancer Patients

Oral cancer is the most common form of carcinoma of oral cavity and ranks as the 12th most common cancer in the world. Oral cancer is one of the major social & health problems in India and Indian subcontinent countries. Tobacco use in different forms is the main etiological factor for oral carcinoma. (i) To define relations of oral cancer with respect to gender, age group, socioeconomic status and risk habits; (ii) To observe the distribution of affected oral cavity sites and observe the clinical profile in oral cancer patients. A cross-sectional study of 340 oral cancer patients from January 2019 to December 2019 was carried out in Department of oncology & radiotherapy, JA Group of hospital, G. R. Medical Gwalior, Madhya Pradesh India. Details of patient's sex, age, tobacco habit and site of cancer and status were noted. Data were analyzed by probability of patient with risk factors and chi-squire (χ) test. The prevalence of oral cancer was significantly (p &#60; 0.001) higher in males (91.5.9%) than females (8.5%). In both the sexes, most affected sites were the buccal mucosa and gingivo-buccal sulcus. However, the consuming form of tobacco was found to be significantly associated with oral cancer, in both sex. The study revealed that oral cancer is more common in men, probably due to habit of large tobacco consumption.

scholarly journals Evaluating the epidemiology and needs of oral cancer patients from Aurangabad district, Maharashtra, India

2017 ◽  
Vol 5 (7) ◽  
pp. 2905 ◽  
Author(s):  
Vikram R. Niranjan ◽  
Sudarshan G. Ranpise
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Tobacco Consumption ◽  
Public Health Issue ◽  
P Value ◽  
Psychological Counseling ◽  
Female Ratio ◽  
Male Female Ratio ◽  
Epidemiologic Profile ◽  
Oral Cancer Patients

Background: India is facing burden of non-communicable diseases and oral cancer is one of the leading public health issue. This is a descriptive study from Aurangabad district, Maharashtra, India to evaluate epidemiologic profile of oral cancer patients who underwent treatment during 2012 to 2016.Methods: Demographic and clinical profile of 500 patients was recorded with validated questionnaire.Results: With male: female ratio was 2.90:1; the mean age of the patients of oral cancer was 47.73 years. Tobacco consumption was the leading cause associated with statistically significant association (p value < 0.000) among the gender and tobacco use suggesting men are more prone to use tobacco than women. 74% patients received satisfactory treatment and all the patients strongly reported need of doctor’s advice, family support, discontinuation of substance abuse habits and financial help. Patients reported the need of acceptance by society and good diet and nutrition. However, many denied the need of psychological counseling and regular check-up. There was statistically significant association (p value < 0.001) found among the gender and needs for good diet and nutrition, psychological counseling, regular check-up, discontinuation of tobacco habits. Statistically significant association (p value < 0.001) found among the occupation and needs for good diet and nutrition, psychological counseling and acceptance by society.Conclusions: The study projects the epidemiology and focuses on the needs of the patients which require the specific attention and efforts through patient’s education and awareness.

scholarly journals High Expression of KLF10 Is Associated with Favorable Survival in Patients with Oral Squamous Cell Carcinoma

Medicina ◽  
2020 ◽  
Vol 57 (1) ◽  
pp. 17
Author(s):  
Chung-Min Yeh ◽  
Yi-Ju Lee ◽  
Po-Yun Ko ◽  
Yueh-Min Lin ◽  
Wen-Wei Sung
Keyword(s):  
Survival Rate ◽  
Oral Cancer ◽  
Cancer Patients ◽  
Protective Factor ◽  
Female Gender ◽  
Vital Role ◽  
Cancer Stage ◽  
Independent Prognostic Marker ◽  
Oral Cancer Patients ◽  
The Impact

Background and objectives: Krüppel-like transcription factor 10 (KLF10) plays a vital role in regulating cell proliferation, including the anti-proliferative process, activation of apoptosis, and differentiation control. KLF10 may also act as a protective factor against oral cancer. We studied the impact of KLF10 expression on the clinical outcomes of oral cancer patients to identify its role as a prognostic factor in oral cancer. Materials and Methods: KLF10 immunoreactivity was analyzed by immunohistochemical (IHC) stain analysis in 286 cancer specimens from primary oral cancer patients. The prognostic value of KLF10 on overall survival was determined by Kaplan–Meier analysis and the Cox proportional hazard model. Results: High KLF10 expression was significantly associated with male gender and betel quid chewing. The 5-year survival rate was greater for patients with high KLF10 expression than for those with low KLF10 expression (62.5% vs. 51.3%, respectively; p = 0.005), and multivariate analyses showed that high KLF10 expression was the only independent factor correlated with greater overall patient survival. The significant correlation between high KLF10 expression and a higher 5-year survival rate was observed in certain subgroups of clinical parameters, including female gender, non-smokers, cancer stage T1, and cancer stage N0. Conclusions: KLF10 expression, detected by IHC staining, could be an independent prognostic marker for oral cancer patients.

scholarly journals Genetic Variants of lncRNA GAS5 Are Associated with the Clinicopathologic Development of Oral Cancer

2021 ◽  
Vol 11 (5) ◽  
pp. 348
Author(s):  
Ming-Hong Hsieh ◽  
Hsueh-Ju Lu ◽  
Chiao-Wen Lin ◽  
Chia-Yi Lee ◽  
Shang-Jung Yang ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Oral Cancer ◽  
Cancer Patients ◽  
Alcohol Drinking ◽  
Tumor Size ◽  
Genetic Variants ◽  
Tumor Stage ◽  
Advanced Tumor Stage ◽  
Advanced Tumor ◽  
Oral Cancer Patients

The long noncoding RNA, Growth arrest-specific 5 (GAS5) plays a crucial role in the development of oral cancer. However, potential genetic variants in GAS5 that affect the susceptibility and progression of oral cancer have rarely been explored. In this study, two loci of GAS5 single nucleotide polymorphisms (SNPs) (rs145204276 and rs55829688) were genotyped by using the TaqMan allelic discrimination in 1125 oral cancer patients and 1195 non-oral-cancer individuals. After statistical analyses, the distribution of both the GAS5 SNP rs145204276 and GAS5 SNP rs55829688 frequencies were similar between the study and control groups. However, the patients with GAS5 SNP rs145204276 variants (Ins/Del or Del/Del) showed a higher tendency of moderate to poor cell differentiation of oral cancer (OR: 1.454, 95% CI: 1.041–2.031, p = 0.028). Moreover, the GAS5 SNP rs145204276 variants (Ins/Del or Del/Del) in the non-alcohol-drinking population were associated with significantly advanced tumor stage (OR: 1.500, 95% CI: 1.081–2.081, p = 0.015) and larger tumor size (OR: 1.494, 95% CI: 1.076–2.074, p = 0.016). Furthermore, individuals with the GAS5 SNP rs145204276 variant were associated with a higher expression of GAS5 in the GTEx database (p = 0.002), and the higher GAS5 level was associated with poor cell differentiation, advanced tumor stage and larger tumor size in head and neck squamous cell carcinoma from the TCGA database (all p < 0.05). In conclusion, the GAS5 SNP rs145204276 variant is related to poor-differentiation cell status in oral cancer. Besides, the presence of the GAS5 SNP rs145204276 variant is associated with a worse tumor stage and tumor size in oral cancer patients without alcohol drinking.

scholarly journals Adjuvant Radiotherapy Significantly Increases Neck Control and Survival in Early Oral Cancer Patients with Solitary Nodal Involvement: A National Cancer Registry Database Analysis

Cancers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 3742
Author(s):  
Chia-Jen Tsai ◽  
Yu-Hsuan Kuo ◽  
Hung-Chang Wu ◽  
Chung-Han Ho ◽  
Yi-Chen Chen ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Cancer Registry ◽  
Adjuvant Radiotherapy ◽  
Multivariable Analysis ◽  
Nodal Involvement ◽  
Cox Regression Analysis ◽  
Oral Cancer Patients ◽  
Neck Control ◽  
Registry Database

We assessed the role of adjuvant radiotherapy on neck control and survival in patients with early oral cancer with solitary nodal involvement. We identified pT1-2N1 oral cancer patients with or without adjuvant radiotherapy from the 2007–2015 Taiwan Cancer Registry database. The effect of adjuvant radiotherapy on 5-year neck control, overall survival (OS) and disease-free survival (DFS) were calculated using the Kaplan–Meier method, log-rank tests, and Cox regression analysis. Of 701 patients identified, 505 (72.0%) received adjuvant radiotherapy and 196 (28.0%) had surgery alone. Patients receiving adjuvant radiotherapy were more likely to be aged <65 years, pT2 stage, poorly graded and without comorbid conditions (all, p < 0.05). The 5-year OS and DFS differed significantly by receipt of adjuvant radiotherapy. Multivariable analysis showed adjuvant radiotherapy significantly associated with better 5-year OS (adjusted hazard ratio (aHR), 0.72; 95% confidence interval (CI), 0.54–0.97; p = 0.0288) and DFS (aHR, 0.64; 95% CI, 0.48–0.84; p = 0.0016). Stratified analysis indicated the greatest survival advantage for both 5-year OS and DFS in those with pT2 classification (p = 0.0097; 0.0009), and non-tongue disease (p = 0.0195; 0.0158). Moreover, adjuvant radiotherapy significantly protected against neck recurrence (aHR, 0.30; 95% CI, 0.18–0.51; p < 0.0001). Thus, adjuvant radiotherapy is associated with improved neck control and survival in these early oral cancer patients.

scholarly journals Clinical long-term and patient-reported outcomes of dental implants in oral cancer patients

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Eik Schiegnitz ◽  
Lena Katharina Müller ◽  
Keyvan Sagheb ◽  
Lisa Theis ◽  
Vahide Cagiran ◽  
...  
Keyword(s):  
Radiation Therapy ◽  
Oral Cancer ◽  
Cancer Patients ◽  
Dental Implants ◽  
Survival Rates ◽  
Implant Survival ◽  
Implant Placement ◽  
Patient Reported ◽  
Oral Cancer Patients

Abstract Background and purpose The aim of this clinical study was to investigate the clinical long-term and patient-reported outcome of dental implants in patients with oral cancer. In addition, analysis of the influence of radiation therapy, timing of implant insertion, and augmentation procedures on implant survival was performed. Material and methods This retrospective study investigated the clinical outcome of 711 dental implants in 164 oral cancer patients, inserted by experienced surgeons of the Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Germany. Oral health-related quality of life (OHRQoL) was evaluated. Results Cumulative 5-year and 10-year implant survival rates for all included implants were 87.3% and 80.0%. Implants placed straight after ablative surgery (primary implant placement) and implants placed after completing the oncologic treatment (secondary implant placement) showed a comparable implant survival (92.5% vs. 89.5%; p = 0.635). Irradiation therapy had no significant influence on implant survival of secondary placed implants (p = 0.929). However, regarding implant site (native bone vs. augmented bone) and radiation therapy (non-irradiated bone vs. irradiated bone), implants inserted in irradiated bone that received augmentation procedures showed a statistically significant lower implant survival (p < 0.001). Patients reported a distinct improvement in OHRQoL. Conclusions Promising long-term survival rates of dental implants in patients after treatment of oral cancer were seen. In addition, patients benefit in form of an improved OHRQoL. However, bone augmentation procedures in irradiated bone may result in an impaired implants’ prognosis.

Sign in / Sign up

Export Citation Format

Share Document