Localization and activity of iNOS in normal human lung tissue and lung cancer tissue

2007 ◽  
Vol 22 (3) ◽  
pp. 226-231 ◽  
Author(s):  
L. Speranza ◽  
M.A. De Lutiis ◽  
Y.B. Shaik ◽  
M. Felaco ◽  
A. Patruno ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lung Tissue ◽  
Human Lung ◽  
Cancer Tissue ◽  
Lung Cancer Tissue ◽  
Human Lung Tissue ◽  
Normal Human

Localization and Activity of iNOS in Normal Human Lung Tissue and Lung Cancer Tissue

2007 ◽  
Vol 22 (3) ◽  
pp. 226-231 ◽  
Author(s):  
L. Speranza ◽  
M.A. De Lutiis ◽  
Y.B. Shaik ◽  
M. Felaco ◽  
A. Patruno ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Lung Cancer ◽  
Tumor Tissue ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Cancer Tissue ◽  
Lung Cancer Tissue ◽  
Human Lung Tissue ◽  
Normal Human ◽  
High Concentrations

Inducible nitric oxide synthase (iNOS) is one of three enzymes generating nitric oxide (NO) from the amino acid L-arginine. iNOS-derived NO plays an important role in several physiological and pathophysiological conditions. NO is a free radical which produces many reactive intermediates that account for its bioactivity. In the human lung, the alveolar macrophage is an important producer of cytokines and this production may be modified by NO. Moreover, high concentrations of NO have been shown to increase nuclear factor KB (NF-kB) activation. Recent investigations of NO expression in tumor tissue indicated that, at least for certain tumors, NO may mediate one or more roles during the growth of human cancer. We have studied iNOS in two tissue groups: normal human lung tissue and human lung cancer tissue. We localized iNOS in these tissues by immunohistochemistry and tested the mRNA expression by RT-PCR, the protein level by Western blot, and the protein activity by radiometric analysis. The results demonstrate different expression, localization and activity of iNOS in normal versus tumor tissue. This is suggestive of a role for NO production from iNOS in human lung cancer because high concentrations of this short molecule may transform to highly reactive compounds such as peroxynitrite (ONOO-); moreover, through the upregulator NF-kB, they can induce a chronic inflammatory state representing an elevated risk for cell transformation to cancer.

Effect of Benoxaprofen on Release of Slow-Reacting Substances from Human Lung Tissue In Vitro

1982 ◽  
Vol 63 (2) ◽  
pp. 219-221 ◽  
Author(s):  
V. Y. Lee ◽  
J. Margaret Hughes ◽  
J. P. Seale ◽  
Diana M. Temple
Keyword(s):  
Lung Cancer ◽  
Lung Tissue ◽  
Human Lung ◽  
Calcium Ionophore ◽  
Inhibitory Effects ◽  
Human Lung Tissue ◽  
Normal Human ◽  
Clinical Asthma

1. Macroscopically normal human lung tissue was obtained from operative specimens removed for lung cancer and challenged with antigen or calcium ionophore. The release of histamine and slow-reacting substances was measured by fluorimetric and bioassay techniques respectively. 2. Benoxaprofen, a drug with inhibitory effects on the lipoxygenase and cyclo-oxygenase pathways, caused a dose-related reduction of release of slow-reacting substances without affecting histamine release. 3. These results with human lung tissue in vitro suggest that benoxaprofen may be used to investigate the role of slow-reacting substances in experimental and clinical asthma.

scholarly journals AZGP1 mRNA levels in normal human lung tissue correlate with lung cancer disease status

Oncogene ◽  
2007 ◽  
Vol 27 (11) ◽  
pp. 1650-1656 ◽  
Author(s):  
F S Falvella ◽  
M Spinola ◽  
C Pignatiello ◽  
S Noci ◽  
B Conti ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lung Tissue ◽  
Human Lung ◽  
Disease Status ◽  
Mrna Levels ◽  
Human Lung Tissue ◽  
Cancer Disease ◽  
Normal Human

Role of DACH1 on proliferation, invasion, and apoptosis in human lung adenocarcinoma cells

2021 ◽  
Vol 21 ◽  
Author(s):  
Junjie Yu ◽  
Ping Jiang ◽  
Ke Zhao ◽  
Zhiguo Chen ◽  
Tao Zuo ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lung Adenocarcinoma ◽  
Tumor Cells ◽  
Human Lung ◽  
A549 Cells ◽  
Cancer Tissue ◽  
Lung Cancer Tissue ◽  
Adenocarcinoma Cells ◽  
Human Lung Adenocarcinoma Cells ◽  
Lung Adenocarcinoma Cells

Objective: To investigate DACH1 protein expression in lung cancer tissue and matched paracancerous tissue, and explore its effect on proliferation, invasion, and apoptosis in human lung adenocarcinoma cells (HLACs). Methods: Tumor tissue and matched paracancerous tissue was collected from 46 patients with pathologically diagnosed lung cancer. RT-PCR was perfomed to detect DACH1 mRNA expression and immunohistochemistry to measured DACH1 protein expression. To determine the effect of DACH1 on lung cancer behavior, small interfering RNA (siRNA) was used to silence DACH1 expression in A549 cells. The impact on the proliferation of tumor cells was then observed by MTT assay, changes in the invasion of tumor cells were identified using transwell chamber assay, and the effects on apoptosis in the cell line were detected using flow cytometry. Results: The expression of DACH1 mRNA and DACH1 protein were significantly decreased in lung cancer tissue versus matched paracancerous control tissue. Silencing of DACH1 expression in A549 cells significantly enhanced cell proliferation, significantly increased cell invasion and significantly reduced spontaneous apoptosis. Conclusion: DACH1 is downregulated in lung adenocarcinoma tissue. In vitro assessment shows that DACH1 functions as a tumor suppressor, suggesting its potential use as new target for lung cancer treatment.

Elemental analyses in human lung tissue correlated with smoking, emphysema and lung cancer

1989 ◽  
Vol 19 (1-2) ◽  
pp. 1-6 ◽  
Author(s):  
P‐L. Kalliomäki ◽  
P. Kokkonen ◽  
P. Pääkkö ◽  
S. Anttila ◽  
K. Kalliomäki
Keyword(s):  
Lung Cancer ◽  
Lung Tissue ◽  
Human Lung ◽  
Human Lung Tissue ◽  
Elemental Analyses

scholarly journals Distribution of phospholipase C isozymes in normal human lung tissue and their immunohistochemical localization

1996 ◽  
Vol 11 (4) ◽  
pp. 305 ◽  
Author(s):  
Sung Chul Hwang ◽  
Kwang Hwa Park ◽  
Mahn Joon Ha ◽  
In Sook Noh ◽  
Tae Byung Park ◽  
...  
Keyword(s):  
Phospholipase C ◽  
Lung Tissue ◽  
Human Lung ◽  
Immunohistochemical Localization ◽  
Human Lung Tissue ◽  
Normal Human

scholarly journals CK2 Enzyme Affinity Against c-myc424-434Substrate in Human Lung Cancer Tissue

2012 ◽  
Vol 13 (10) ◽  
pp. 5233-5236
Author(s):  
Ilhan Yaylim ◽  
Nazli Ezgi Ozkan ◽  
Turgut Isitmangil ◽  
Gulbu Isitmangil ◽  
Akif Turna ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Cancer Tissue ◽  
Lung Cancer Tissue ◽  
Human Lung Cancer Tissue
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