Phenotype of peripheral blood leukocytes and survival of patients with metastatic colorectal cancer

2005 ◽  
Vol 20 (2) ◽  
pp. 126-133 ◽  
Author(s):  
P. Vesely ◽  
M. Touskov ◽  
B. Melichar
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes

Phenotype of Peripheral Blood Leukocytes and Survival of Patients with Metastatic Colorectal Cancer

2005 ◽  
Vol 20 (2) ◽  
pp. 126-133 ◽  
Author(s):  
P. Veselý ◽  
M. Touškoví ◽  
B. Melichar
Keyword(s):  
Colorectal Cancer ◽  
Regression Analysis ◽  
Metastatic Colorectal Cancer ◽  
Peripheral Blood ◽  
Prognostic Significance ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Color Flow ◽  
Cancer Metastases ◽  
Colorectal Cancer Metastases

Immune dysfunction is prevalent in metastatic cancer. Few patients with colorectal cancer metastases are cured, and among the strategies aimed at improving the therapeutic results in patients with metastatic colorectal cancer, immunotherapy is being increasingly investigated. We evaluated retrospectively the prognostic significance of peripheral blood leukocytes in 59 patients with metastatic colorectal cancer. The relative numbers of CD3+, CD3+CD4+, CD3+CD8+, NK (CD3-CD16+CD56+), CD3+DR+, CD3+CD25+, CD3+CD69+, CD19+, CD19+CD23+, CD8+CD28+, CD8-CD28+, CD8+CD57+, CD14+DR+ and CD14+CD16+ leukocytes were analyzed by two-color flow cytometry. A three-step approach was adopted to identify predictors of prognosis using regression analysis. Based on the results of univariate survival analysis, the absolute number of white blood cells, NK/CD3+CD69+ and NK/white cell count ratios were significant indicators of prognosis. In the multivariate regression analysis a model was obtained using a single parameter, the NK/CD3+CD69+ ratio, predicting the survival with 10–15% power of regression. The present results indicate that the NK/CD3+CD69+ ratio in peripheral blood may be an independent variable in a regression model predicting the overall survival of patients with colorectal cancer metastases to be tested in prospective studies.

A Dinucleotide Deletion in the CD24 Gene Is a Potential Risk Factor for Colorectal Cancer

2020 ◽  
Vol 86 (5) ◽  
pp. 480-485
Author(s):  
Lior Segev ◽  
Ilana Naboishchikov ◽  
Diana Kazanov ◽  
Ezra Bernstein ◽  
Meital Shaked ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Peripheral Blood ◽  
Intracellular Signaling ◽  
Genetic Variant ◽  
Colorectal Carcinogenesis ◽  
Polymorphism Analysis ◽  
Peripheral Blood Leukocytes ◽  
Clinical Value ◽  
Blood Leukocytes ◽  
Multistep Process

Background CD24 is a sialoglycoprotein anchored to the cell surface via glycosylphosphatidylinositol and is involved in intracellular signaling processes. It plays an important role in the early stages of the multistep process of colorectal carcinogenesis. Several single nucleotide polymorphisms in the CD24 gene are reported to exert a diverse effect on cancer risk. We aimed to elucidate whether CD24 TG/del genetic variants are associated with susceptibility to colorectal cancer (CRC). Methods The study included 179 subjects, 36 with CRC (prior to surgery) and 143 healthy control subjects. Deoxyribonucleic acid was purified from peripheral blood leukocytes, and by using restriction fragment length polymorphism analysis, the CD24 gene was genotyped for the specific genetic variant, TG deletion. Additionally, CD24 protein expression levels were determined by Western blotting analysis. Results The incidence of the TG/del was higher among the CRC patients compared with healthy controls, 14% and 10%, respectively ( P = .54). CD24 protein levels were significantly higher among CRC patients. There were no significant differences in CD24 expression between CRC patients at different stages of the disease or between patients who carry the mutation and those who did not. Conclusions CD24 genetic variant might be of clinical value for risk assessment as part of cancer prevention programs. Further study on larger populations is needed to validate the importance of this dinucleotide deletion in CRC development. Overexpression of CD24 protein occurs early along the multistep process of CRC carcinogenesis, and a simple blood sample based on CD24 expression on peripheral blood leukocytes can contribute to early diagnosis.

scholarly journals Methylation of a panel of genes in peripheral blood leukocytes is associated with colorectal cancer

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xiang Luo ◽  
Rong Huang ◽  
Hongru Sun ◽  
Yupeng Liu ◽  
Haoran Bi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes

Oncogene and Tumor Suppressor Gene Expression Changes in the Peripheral Blood Leukocytes of Patients with Colorectal Cancer

2008 ◽  
Vol 94 (1) ◽  
pp. 79-82 ◽  
Author(s):  
Zsolt Csontos ◽  
Edit Nádasi ◽  
András Csejtey ◽  
László Illényi ◽  
Miklós Kassai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Tumor Suppressor ◽  
Tumor Suppressor Gene ◽  
Peripheral Blood ◽  
Suppressor Gene ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes

scholarly journals Telomere Length in Peripheral Blood Leukocytes Is Associated with Risk of Colorectal Cancer in Chinese Population

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e88135 ◽  
Author(s):  
Qin Qin ◽  
Jingwen Sun ◽  
Jieyun Yin ◽  
Li Liu ◽  
Jigui Chen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Telomere Length ◽  
Peripheral Blood ◽  
Chinese Population ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes
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