Assessment and in vitro experiment of artificial anal sphincter system based on rebuilding the rectal sensation function

2014 ◽  
Vol 37 (5) ◽  
pp. 392-401 ◽  
Author(s):  
Peng Zan ◽  
Jinding Liu ◽  
Enyu Jiang ◽  
Hua Wang
Keyword(s):  
Anal Sphincter ◽  
Artificial Anal Sphincter ◽  
Rectal Sensation ◽  
Vitro Experiment ◽  
In Vitro Experiment

In Silico and in Vitro Evaluation of Deamidation Effects on the Stability of the Fusion Toxin DAB389IL-2

2019 ◽  
Vol 16 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Nasrin Zarkar ◽  
Mohammad Ali Nasiri Khalili ◽  
Fathollah Ahmadpour ◽  
Sirus Khodadadi ◽  
Mehdi Zeinoddini
Keyword(s):  
In Silico ◽  
Catalytic Domain ◽  
Md Simulations ◽  
Special Importance ◽  
Native Form ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Pharmaceutical Protein ◽  
The Stability

Background: DAB389IL-2 (Denileukin diftitox) as an immunotoxin is a targeted pharmaceutical protein and is the first immunotoxin approved by FDA. It is used for the treatment of various kinds of cancer such as CTCL lymphoma, melanoma, and Leukemia but among all of these, treatment of CTCL has special importance. DAB389IL-2 consists of two distinct parts; the catalytic domain of Diphtheria Toxin (DT) that genetically fused to the whole IL-2. Deamidation is the most important reaction for chemical instability of proteins occurs during manufacture and storage. Deamidation of asparagine residues occurs at a higher rate than glutamine residues. The structure of proteins, temperature and pH are the most important factors that influence the rate of deamidation. Methods: Since there is not any information about deamidation of DAB389IL-2, we studied in silico deamidation by Molecular Dynamic (MD) simulations using GROMACS software. The 3D model of fusion protein DAB389IL-2 was used as a template for deamidation. Then, the stability of deamidated and native form of the drug was calculated. Results: The results of MD simulations were showed that the deamidated form of DAB389IL-2 is more unstable than the normal form. Also, deamidation was carried by incubating DAB389IL-2, 0.3 mg/ml in ammonium hydrogen carbonate for 24 h at 37o C in order to in vitro experiment. Conclusion: The results of in vitro experiment were confirmed outcomes of in silico study. In silico and in vitro experiments were demonstrated that DAB389IL-2 is unstable in deamidated form.

Effect of bleaching on ethyl glucuronide in hair: An in vitro experiment

2010 ◽  
Vol 198 (1-3) ◽  
pp. 23-27 ◽  
Author(s):  
Luca Morini ◽  
Alessandra Zucchella ◽  
Aldo Polettini ◽  
Lucia Politi ◽  
Angelo Groppi
Keyword(s):  
Ethyl Glucuronide ◽  
Vitro Experiment ◽  
In Vitro Experiment

scholarly journals A review of biocompatibility of zirconia: In vitro experiment

2018 ◽  
Vol 56 (4) ◽  
pp. 391 ◽  
Author(s):  
Da-Won Suh ◽  
Young-Kyun Kim ◽  
Yang-Jin Yi
Keyword(s):  
Vitro Experiment ◽  
In Vitro Experiment

scholarly journals Stratified Community Responses to Methane and Sulfate Supplies in Mud Volcano Deposits: Insights from an In Vitro Experiment

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e113004 ◽  
Author(s):  
Yu Zhang ◽  
Lois Maignien ◽  
Alina Stadnitskaia ◽  
Pascal Boeckx ◽  
Xiang Xiao ◽  
...  
Keyword(s):  
Mud Volcano ◽  
Community Responses ◽  
Vitro Experiment ◽  
In Vitro Experiment

scholarly journals Fast low-angle positive contrast steady-state free precession imaging of USPIO-labeled macrophages: theory and in vitro experiment

2009 ◽  
Vol 27 (7) ◽  
pp. 961-969 ◽  
Author(s):  
Nicole Mascheri ◽  
Rohan Dharmakumar ◽  
Zhuoli Zhang ◽  
Tatjana Paunesku ◽  
Gayle Woloschak ◽  
...  
Keyword(s):  
Steady State ◽  
Steady State Free Precession ◽  
Positive Contrast ◽  
Free Precession ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Free Precession Imaging

Comparison of Carotid Stents: An In-Vitro Experiment Focusing on Stent Design

2009 ◽  
Vol 16 (2) ◽  
pp. 168-177 ◽  
Author(s):  
Stefan Müller-Hülsbeck ◽  
Philipp J. Schäfer ◽  
Nikolas Charalambous ◽  
Silvio R. Schaffner ◽  
Martin Heller ◽  
...  
Keyword(s):  
Stent Design ◽  
Vitro Experiment ◽  
In Vitro Experiment
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