scholarly journals Effects of water pH on physiological parameters and color changes of Asian Bumblebee Catfish (Pseudomystus siamensis Regan, 1913)

2019 ◽  
Vol 02 ◽  
pp. 78-87
Author(s):  
Tuan V. Vo

Effects of water pH on blood physiological parameters and color change of Asian bumblebee catfish (Pseudomystus siamensis) (4 – 6 g/fish) were carried out in laboratory condition. The experiment was set up in 8 weeks at different pH water levels (pH = 3, 4, 5, 6, 7, 8, 9, 10, 11). The results have shown that the cumulative mortality ratio of Asian bumblebee catfish at the end of 24 h challenge was 100% at pH = 11, 70.83% at pH = 10, and 62.5% at pH = 3. No mortality of fish was observed at pH = 4, 5, 6, 7, 8, 9 after 24 h of the challenge. The lowest and highest pH threshold that killed 50% of fish after 24 h of the challenge were 3.04 and 9.95, respectively. After 24 h of the challenge, total number of red and white blood cells of fish increased at pH = 3, 9, 10, and get the highest level at pH = 3 (1.87 × 106 cells/mm3 and 1.59 × 105 cells/mm3, respectively. At the end of the challenge, highest number of red and white blood cells were observed at pH = 8 (2 ± 0.23 × 106 cells/mm3 and 1.27 ± 0.26 × 105 cells/mm3, respectively). Fish were in bright and beautiful color when cultured in high pH water levels.

1952 ◽  
Vol 8 (2) ◽  
pp. 102-125 ◽  
Author(s):  
Z. Z. GODLOWSKI

1. It is suggested that some of the circulating blood eosinophils are originally produced in the epithelial lining of the lower parts of the intestinal crypts, mainly in the small intestine but to a lesser extent in the colon. This cellular reaction may result from the penetration by the antigenic unsplit protein molecule from the lumen of the intestine into the epithelial cells, transforming these into new histological and biological units. 2. This process takes place in the normal intestinal mucosa. It is strongly intensified by the ingestion of antigenic proteins and various traumata inflicted on the intestinal canal. 3. White mice fed with de-antigenized proteins do not form a lymphocytic barrier in the lamina propria mucosae; eosinophils do not appear there until antigenic proteins are introduced into the diet. 4. Heparinization to a higher level than is required for the prevention of blood clotting, done prior to the injection of hormones causing eosinopenia, abolishes or significantly diminishes circulating eosinopenia or may actually increase the number of circulating eosinophils. Fluctuations result from two independent actions: mobilization of tissue eosinophils by heparin, and destruction of eosinophils by steroids in the circulating blood. 5. The eosinolytic action of adrenocortical steroids results from mediation in the catabolic phase of the intracellular metabolism of proteins. This action has been demonstrated in all white cells of the blood. 6. Hormonally induced eosinopenia, in men and in dogs, results from the eosinolysis which takes place in the blood. The other white blood cells are also affected by the same action. The final number of polymorphs is masked by their mobilization set up by cortical steroids through some unknown mechanism. Lymphocytes are less sensitive than eosinophils to the lytic action of cortical steroids.


1989 ◽  
Vol 37 (6) ◽  
pp. 627 ◽  
Author(s):  
C Kemper ◽  
DJ Kitchener ◽  
WF Humphreys ◽  
RA How ◽  
LH Schmitt ◽  
...  

Breeding, population dynamics and seasonal changes in physical and physiological parameters were examined in Isoodon macrourus at the Mitchell Plateau between September 1981 and November 1982. Females gave birth to litters of 2.5 � 1.0 (16) young between September and April. They produced up to three litters in a breeding season with an average interval between successive litters of 89.5 (51-108) days. Adult testosterone levels and scrota1 size began to increase in July and peaked in September 1982. The overall ratio of adult males to adult females was 1:0.54; it was 1:0.80 for pouch young. Females were more frequently trapped on sequential trips than were males. Density on the grids increased towards the end of the dry season (July and September 1982)-mostly as a result of increased numbers of females. Adult males and females moving onto the grids during the dry season tended to select different habitats. Adult males moved significantly more in the wet season (September 1981, January and April) than they did in the dry season (July and September 1982); they also moved significantly more than adult females in the wet season. Seasonal variations were recorded for most physical and physiological parameters. During the wet period from September 1981 to January 1982, body weight, haemoglobin, haematocrit and total plasma albumin declined, while total white blood cells and proportion of lymphocytes to granular leucocytes rose. This indicated that this period was one of change in condition for this species. Compared to adult females, adult males were larger and heavier, had higher values of haematocrit and lymphocytes, and lower values of granular leucocytes, free steroids, CBGBd and albumin bound corticosteroid. There was considerable between-year variability in condition of individuals and both sexes differed significantly in September 1981 and 1982 in their weight, haematocrit, total white blood cells, granular leucocytes, MCBC and testosterone. Individuals at disparate localities at the Mitchell Plateau showed similar seasonal physiological and physical responses, except for one grid where they were heavier.


2019 ◽  
Vol 02 ◽  
pp. 88-96
Author(s):  
Tuan V. Vo

Effect of water pH on susceptibility of whiteleg shrimp Litopenaeus vannamei to acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus was carried out in laboratory condition. White leg shrimp (2 - 3 g) were challenged by immersion for 2 h with tryptic soy broth (TSB)-grown Vibrio parahaemolyticus at 10 times lower dose of LD50. The results showed that the cumulative mortality of V. parahaemolyticus-immersed shrimp after 240 h was increased from low to high pH water levels (23.3 ± 5.8% in pH 6.3; 30.0 ± 20.0% in pH 7.3; 86.7 ± 15.3 in pH 9.3, respectively). The cumulative mortality of shrimp that held in pH = 8.3 was the lowest (20.0 ± 0.0%). In another experiment, immune parameters such as total haemocytes count and respiratory burst of Litopenaeus vannamei held at different pH levels were examined at 0, 24, 48, 72 and 96 h. The results indicated that no significant difference of total haemocytes count was observed at different pH water levels (pH 6.3, 7.3, 8.3, 9.3) at 0 - 72 hpc (hour post challenge). At 96 hpc, total haemocytes count at high pH water level (9.3) was increased and significant difference in comparison with the total haemocytes count recorded in low pH water levels (6.3, 7.3, 8.3). Respiratory burst was also not different at different pH water levels at 0 hpc. However, respiratory burst of shrimp that held at low pH water levels (pH 6.3 and 7.3) was rapidly reduced and significant difference in compared with the shrimp that held in high pH water levels (pH 8.3 and 9.3). It was therefore concluded that low and high pH stress decrease the resistance of Litopenaeus vannamei against V. parahaemolyticus and decrease several parameters of the immune response.


2019 ◽  
Vol 8 (4) ◽  
pp. 54-60
Author(s):  
A. N. Torgashova ◽  
M. A. Barabanova ◽  
V. A. Porkhanov ◽  
L. V. Timchenko ◽  
O. N. Zhadan ◽  
...  

Christian–Weber disease refers to the group of panniculitis, which present an inflammation of the subcutaneous adipose tissue with the involvement of neutrophils, white blood cells and histiocytics, which leads to fibrosis in adipose tissue and sometimes to granulomatous changes. Clinically this pathology manifests from the formation of nodes in the subcutaneous adipose tissue, which appear suddenly, sporadically in some areas or multiple, sometimes forming large aggregations (plaques) or strands, shaped like a rosary. Consistency of nodes initially soft, then compacted, can form adhesions with the skin, the skin over them is not changed initially, followed by a color change from bright pink to brown-purple. Ends the process of atrophy of the affected area, sometimes with a depression in the center. Localization sites in the body are different; they are placed often on the buttocks, arms and legs. The duration of the process of the skin varies from a few days to a few months, after involution of old nodes may appear new ones, it are the cause of increasing the duration of the disease, having a relapsing course. It can be rather difficult to do differential diagnosis, which requires a great deal of experience of experts and a team approach to the study and treatment of this pathology. To confirm the diagnosis requires a biopsy of the skin. Prognosis depends on the nature of the disease and timely diagnosis.


Author(s):  
Sura Safi Obayes Khafaji

The aim of present research to study the effects of crushed seeds of Coriander (Coriandrum sativum) on some physiological parameters in rabbits. Thirty mature male rabbits, (aged 87-100 days),were divided randomly into three equal groups. First group (control) fed the basal diet along the experimental period,2nd and 3rd groups (T1 and T2) fed the basal diet mixed with 3% and 6%,respectively. At the end of experiment,blood samples were collected from ear vein for counting the red blood cells (RBCs) and white blood cells (WBCs),estimating Hemoglobin concentration (Hb) and packed cell volume (PVC). Total protein,albumin,globulin,blood glucose,GOT and GPT concentrations were estimated in blood serum. The results showed significant increase (P<0.05) of RBCs,WBCs,Hb and PCV levels in T1 and T2 groups when compared with control group. As well as,The results of total protein and globulin increased significantly in two treated groups in comparison with control groups. While,the results of glucose,GOT and GPT levels showed a significant decrement (P<0.05) in T1 and T2 groups compared with control group. Therefore,it was concluded that the use of Coriander seeds powder can affected positively on some physiological traits in rabbits.


2020 ◽  
pp. 129-136
Author(s):  
Asiata Adekilekun Kolawole ◽  
Reuben Ataitiya ◽  
Quazim Olawale Junaid ◽  
Abdur-Rahman Kolawole Mustapha

Esta investigación determina el efecto toxicológico de efluentes domésticos en los parámetros sanguíneos de Clarias gariepinus. Se realizaron cuatro tratamientos: Control, 30, 50 y 70 mlL-1. Cada tratamiento fue por triplicado. Se produjeron cambios de comportamiento en los peces expuestos a 50 y 70 mlL-1 del efluente, y no se observó ninguno en los peces Control y los tratados con 30 mlL-1. Hubo diferencias significativas en la ganancia media de peso y longitud entre los peces expuestos al tratamiento y el control. Hubo una reducción significativa (p < 0.05) de los glóbulos rojos. Los glóbulos blancos, el hematocrito, el volumen corpuscular medio y la hemoglobina de los peces expuestos fueron significativamente más altos que en el control (p < 0,05). Los resultados de este estudio sugieren que los efluentes domésticos pueden afectar. This research determines the toxicological effect of domestic effluents on blood parameters of Clarias gariepinus. Four treatment were set up: Control, 30, 50 and 70mlL-1 . Each treatment was done in triplicate. There were behavioral changes in fishes exposed to 50 and 70 mlL-1 of the effluent while none was observed in Control fishes and fishes undergone 30 mlL-1. There were significant differences in the Mean Weight and Mean Length Gain between the fishes exposed to treatment and Control fishes. There was significant reduction in the red blood cells (p < 0.05). White blood cells, hematocrit, mean corpuscular volume and haemoglobin in the fishes exposed were significantly (p < 0.05) higher than Control. Results from this study suggests that domestic effluents can negatively affect the growth and haematology of fishes.


2019 ◽  
Vol 18 (01) ◽  
pp. 89-97
Author(s):  
Tuan V. Vo

Effects of pH on blood physiological parameters and growth performance of Asian bumblebee catfish (Pseudomystus siamensis) were carried out in laboratory conditions. The experiment was set up in 8 weeks at different pH water levels. The results showed that the cumulative mortality of Asian bumblebee catfish (4 - 6 g/fish) in 24 hpi was 100% at pH = 11, 70.83% at pH = 10 and 62.5% at pH = 3. No mortality of fish was observed at pH = 4, 5, 6, 7, 8, 9 at 24 hpi. The high content of glucose was recorded in fish cultured in water with low (4.02 mmol/L at pH = 3) and high (3.22 mmol/L at pH = 10) pH levels. After 8 weeks of culture, the highest content of glucose was observed at pH = 8 (1.10 mmol/L). The daily weight gain and specific growth rate in weight of fish were 0.02 - 0.08 g/day and 0.35 - 0.99%/day, respectively. The daily length gain specific growth rate in length were 0.02 - 0.04 cm/day and 0.22 - 0.53%/day. The highest survival rate of fish was recorded at pH = 6 (95.96%) and the lowest survival rate of fish was recorded at pH = 8 (60%).


Author(s):  
Delma P. Thomas ◽  
Dianne E. Godar

Ultraviolet radiation (UVR) from all three waveband regions of the UV spectrum, UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm), can be emitted by some medical devices and consumer products. Sunlamps can expose the blood to a considerable amount of UVR, particularly UVA and/or UVB. The percent transmission of each waveband through the epidermis to the dermis, which contains blood, increases in the order of increasing wavelength: UVC (10%) < UVB (20%) < UVA (30%). To investigate the effects of UVR on white blood cells, we chose transmission electron microscopy to examine the ultrastructure changes in L5178Y-R murine lymphoma cells.


1990 ◽  
Vol 63 (01) ◽  
pp. 112-121 ◽  
Author(s):  
David N Bell ◽  
Samira Spain ◽  
Harry L Goldsmith

SummaryThe effect of red blood cells, rbc, and shear rate on the ADPinduced aggregation of platelets in whole blood, WB, flowing through polyethylene tubing was studied using a previously described technique (1). Effluent WB was collected into 0.5% glutaraldehyde and the red blood cells removed by centrifugation through Percoll. At 23°C the rate of single platelet aggregtion was upt to 9× greater in WB than previously found in platelet-rich plasma (2) at mean tube shear rates Ḡ = 41.9,335, and 1,920 s−1, and at both 0.2 and 1.0 µM ADP. At 0.2 pM ADP, the rate of aggregation was greatest at Ḡ = 41.9 s−1 over the first 1.7 s mean transit time through the flow tube, t, but decreased steadily with time. At Ḡ ≥335 s−1 the rate of aggregation increased between t = 1.7 and 8.6 s; however, aggregate size decreased with increasing shear rate. At 1.0 µM ADP, the initial rate of single platelet aggregation was still highest at Ḡ = 41.9 s1 where large aggregates up to several millimeters in diameter containing rbc formed by t = 43 s. At this ADP concentration, aggregate size was still limited at Ḡ ≥335 s−1 but the rate of single platelet aggregation was markedly greater than at 0.2 pM ADP. By t = 43 s, no single platelets remained and rbc were not incorporated into aggregates. Although aggregate size increased slowly, large aggregates eventually formed. White blood cells were not significantly incorporated into aggregates at any shear rate or ADP concentration. Since the present technique did not induce platelet thromboxane A2 formation or cause cell lysis, these experiments provide evidence for a purely mechanical effect of rbc in augmenting platelet aggregation in WB.


2013 ◽  
Author(s):  
Olga Papalou ◽  
Sarantis Livadas ◽  
Athanasios Karachalios ◽  
Nektarios Benetatos ◽  
George Boutzios ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document