scholarly journals Phytoremediation Enhancement of Kenaf Plant (Hibiscus cannabinus L.) Grown on Spent Oil Polluted Soil

2020 ◽  
Vol 11 (1) ◽  
pp. 26
Author(s):  
Olajumoke Oke Fayinminnu

Oil pollution especially Spent Lubricating Oil is a wide environmental problem in Nigeria. It constitutes potential toxic effects on soil, flora, fauna and humans and also making the environment unsightly. This study assessed the potential of kenaf plant as a phytoremediator grown in spent oil polluted soil for a period of 84 days (12 weeks). Pot experiment was conducted in the Nursery site of the Moist Forest Research Station, Forestry Research Institute of Nigeria (FRIN), Ibadan, Oyo State, Nigeria. The treatments: amended (with organic manure) and unamended (without organic manure) soils and three pollution levels (0, 3 and 6%) of spent engine oil, each filled in eight-liter plastic pots with 6 kg soil. Growth parameters: plant height, stem diameter and number of leaves were collected and Total Petroleum Hydrocarbon (TPH) in soil was determined. Data were analyzed using descriptive statistics and ANOVA. Results at 12 weeks (84 days) showed amended (control 0%) treatment having highest plant height (112.17 cm), stem diameter (8.92 cm) and number of leaves (178.43), while 6% unamended level of pollution had the lowest plant height (26.78 cm), stem height (1.62 cm) and number of leaves (8.06). Amended soil had the highest total percentage reduction of TPH (75.90, 90.05 and 90.30%), when compared with unamended soil with lowest values (76.88, 85.18 and 82.36%) at 0, 3 and 6 pollution levels, respectively. Reduction of TPH in the phytoremediation process was in this order of pollution levels 6%> 3%> 0%. This study revealed effectiveness of kenaf with organic amendment in remediating Total Petroleum Hydrocarbon in oil polluted soil, hence a good potential phytoremediator.

Author(s):  
Canan Nilay Duran ◽  
Gizem Demirkaplan ◽  
Sevinç Şener

Passionflower is a member of Passifloraceae family, it can be used as medicinal and ornamental plants in addition to its consumption as fruit in the world. Guava (Psidium guajava L.) fruits, which has a rich nutrient content, are consumed both fresh and processed. Passionfruit and guava plants, which can only be cultivated in temperate southern coasts in our country, have gained commercial importance because of the fruits' export potential, high medical importance and nutrient. This study was carried out to determine the effects of some bioactivator applications on the criteria of sapling growth and development of passionflower and guava plants. The experiment was conducted between 2018-2019 under greenhouse conditions. Saplings obtained from seed germination in Akdeniz University Faculty of Agriculture Research and Experiment Area were used as plant material. 3 different commercial preparations called Messenger, Crop-set and ISR-2000 were used as bioactivators. Plant height (cm), stem diameter (mm) and number of leaves (number / plant) were measured weekly, in order to determine the effect of the applications on the growth and development of saplings. At the end of the study, it was determined that the highest average plant height (10.17 cm), stem diameter (13.53 mm) were obtained in ISR-2000 application. The highest average plant height (11.93 cm), stem diameter (16.44 mm) and number of leaves (9.07 units / plant) were obtained from Messenger application in guava plant. When the results obtained are evaluated, it is recommended that ISR-2000 bioactivator can be applied in passionflower sapling cultivation and Messenger bioactivator can be applied for guava sapling cultivation.


Author(s):  
J. O. Dasetima-Altraide ◽  
D. N. Ogbonna ◽  
T. K. S. Abam ◽  
A. E. Gobo

Aim: To assess the Physicochemical indices of Phytoremediated Crude Oil polluted amended soil using grass plant Cyperus esculentus (Cyp) and Phyllanthus amarus (Phy). Study Design: The study employs experimental design, statistical analysis of the data and interpretation. Place and Duration of Study: Rivers State University demonstration farmland in Nkpolu- Oroworukwo, Mile 3 Diobu area of Port Harcourt, was used for this study. The piece of land is situated at Longitude 4°48’18.50” N and Latitude 6ᵒ58’39.12” E measuring 5.4864 m x 5.1816 m with a total area of 28.4283 square meter. Phytoremediation process monitoring lasted for 240 days; analyses were carried out monthly at 30 days’ interval. Methodology: The study was carried out on Crude Oil Polluted soil (PS) amended with bio-nutrient supplements (Spent Mushroom Substrate (SMS) and selected fungi (Aspergillus niger(AN) andMucor racemosus (MR)) used to stimulate and augment the indigenous microbial population present in a crude oil polluted soil thereby enhancing hydrocarbon reduction in pari per sue with phytoremediation (uptake of Crude oil by test plants) over a period of 240 days. Ten (10) experimental plots (two Control (Unpolluted and polluted soil without amendment) and eight polluted amended/treated plots) employing Randomized Block Design (each having dimensions: 100 x 50 x 30 cm LxBxH); formed and mapped out on agricultural soil and left fallow for 6 days before contamination on the seventh day; after which it was allowed for 21 days for proper contamination and exposure to natural environmental factors (to mimic soil crude oil spill site); thereafter nutrients/organics (biostimulating agents) and bioaugmenting organisms were applied. Baseline studies were carried out on soil profile before and after contamination, major parameters monitored and assayed were Total Petroleum Hydrocarbon (TPH) uptake by plant roots and stem, Polycyclic Aromatic Hydrocarbons (PAHs) and TPH reduction in soil. Other physicochemical properties analyzed in the soil from different plots were pH, Electrical Conductivity, Moisture Content, Total Nitrogen, Available Phosphorus, Potassium, Total Organic Carbon, Plant Height, Iron, Lead and Zinc at regular intervals; days 1, 60, 90, 120, 150, 180, 210 & 240. The rate of phytoremediation was estimated from percentage (%) uptake of Total petroleum hydrocarbon (TPH) in plant roots and stem from day 1 -240; while percentage (%) reduction of TPH and PAHs in soil was estimated from day 1 to the residual at day 240. Results: The test plants decreased significant amount of crude oil as revealed in TPH uptake in their roots and Stem. Mean amount and percentage Total Petroleum Hydrocarbon (TPH) uptake by Cyperus esculentus roots and stem were; 152.33±50.34mg/kg, 12.57±4.16% and 201.13±8.80mg/kg, 13.27±0.58% respectively; while that of Phyllanthus amarusroots and stem were 141.50±35.62mg/kg, 11.68±2.94% and 174.44±19.98mg/kg, 11.51±1.32% respectively; revealing higher Uptake of TPH in plant stem than roots. From the initial TPH contamination value of 5503.00mg/kg, it was observed that plots planted with Cyperus esculentus (TPH 5492.75±76.36mg/kg) showed higher reduction of TPH from soil than those planted with Phyllanthus amarus(TPH 5449.72±18.27mg/kg); while PAHs degradation/reduction showed a reverse trend with plots planted with Phyllanthus amarus (PAHs 28.72±2.74mg/kg; 60.46±5.77%) higher than plots planted with Cyperus esculentus s (PAHs 25.77±2.12mg/kg, 54.24±4.47%). Conclusion: Plots planted with Cyperus esculentus showed higher reduction of TPH from soil than those planted with Phyllanthus amarus while PAHs degradation/reduction in plots planted with Phyllanthus amarus was higher than plots planted with Cyperus esculentus. TPH uptake was higher in plant stems than roots; more so, plots amended with nutrient supplements showed significant higher percentage reduction in hydrocarbon in the polluted soil than unamended polluted soil. It is therefore recommended that Cyperus esculentus is a suitable plant species for phytoremediation of crude oil contaminated soil with high TPH value while Phyllanthus amarusis the best option in phytoremediation of polluted soil with high PAHs value, both in combination with bio-nutrient supplement.


2018 ◽  
Vol 7 (1) ◽  
pp. 6
Author(s):  
Hairin Juanda ◽  
Tutik Nugrahini ◽  
Mahdalena Mahdalena

Effect of NASA Liquid Organic Fertilizer and Compost Fertilizer on Kenaf Plant Growth (Hibiscus cannabinus L). This study aims to determine the effect of NASA POC, compost fertilizer and the interaction of NASA POC and compost fertilizer on kenaf plants. This research was carried out at East Borneo Samarinda Indonesia. with a time of ± 4 months, starting from June to September 2016. The design of the study uses factorial completely randomized design (CRD) consisting of 2 treatments and 4 replications. The treatment consists of 2 factors. The first factor is the administration of NASA POC consisting of 4 levels, namely: j0: Control (without treatment), j1: POC with a concentration of 10 ml / liter of water, j2: POC with a concentration of 15 ml / liter of water, j3: POC with a concentration of 20 ml / liter of water. The second factor is Compost fertilizer consisting of 4 levels, namely: k0: Without the provision of Compost Fertilizer, k1: Composting 500 gram / polybag, k2: Composting Fertilizer 700 grams / polybag, k3: Composting 800 grams / polybag. From the analysis of variance showed that the effect of giving NASA POC (J), compost (K) and the interaction of NASA POC and compost (JK) did not affect the parameters of plant height 30 DAP, 60 DAP and 90 DAP, stem diameter 30 DAP, 60 DAP and 90 DAP, number of leaves 30 DAP, 60 DAP and 90 DAP, leaf area 30 DAP, 60 DAP and 90 DAP. 


2020 ◽  
pp. 28-36
Author(s):  
M. M. Olorukooba ◽  
R. Suleiman ◽  
O. Olukotun ◽  
R. Mohammed ◽  
E. Apene ◽  
...  

Field trials were carried out during 2018 and during 2019 dry season periods at Federal College of Forestry and Mechanization Afaka Kaduna, located between latitude 10° 35”N and longitude 007° 21”E at altitude 644 m above sea level, to determine the effects of organic manure source and weeding frequencies on growth parameters of jute mallow (Corchorus olitorious). The experimental treatments consists of ten combinations of poultry manure + weed free, cow dung + weed free, goat dropping + weed free, poultry manure + weeding at 2 WAT, cow dung + weeding at 2 WAT, goat dropping + weeding at 2 WAT, poultry manure + weeding at 4 WAT, cow dung + weeding at 4 WAT, goat dropping + weeding at 4 WAT and  no organic manure application + no weeding which were laid out in a randomize complete block design (RCBD) and replicated 3 times The growth parameters observed  and measured were crop vigour score, plant height, shoot diameter, number of leaves and number of  primary branches. Result of the study showed that at harvest (8 WAT) all the treatments significantly affected the vigour of jute mallow crop and gave comparable score than the least observed with no nutrient + no weeding as control. The result revealed that plant growth at harvest exhibited significantly taller plant with application of poultry manure + weeding at 2 WAT. In conclusion the result obtained from the study showed that poultry manure at 4 tons /ha + weeding at 4 WAT resulted in taller plant height, wider shoot diameter, increased number of leaves, number of branches than all other treatment due to lower weed infestation, it is therefore recommended as the best treatment combination to obtained the best growth rate of jute mallow.


2019 ◽  
Vol 17 (1) ◽  
pp. 175-187
Author(s):  
R Rai ◽  
J Shrestha ◽  
JH Kim

An experiment was carried out using eight double cross F1s (DCF1s) as lines and two testers to obtain sixteen Line ×Tester hybrids for the evaluation of major growth and flowering traits thereby to understand the breeding potentiality of the parental lines. The performance of growth and flowering traits of all twenty-six genotypes were evaluated in a randomized complete block design (RCBD) with three replications. The analysis of variance (ANOVA) showed all the genotypes were significantly different for all agro-morphological traits under study. The estimated general combining ability (GCA) effects showed that parent 5(P5), were superior for plant height, stem diameter, number of leaves, leaf length, days to flowering and attitude of floral axis followed by parent 2(P2) for leaf length, leaf width, days to flowering and attitude of floral axis and parent7 (P7) for number of flower, flower diameter, bud length and attitude for floral axis. Likewise; tester 1 found to be best combiner with significant GCA effect for almost all growth and flowering traits (except number of leaves). The plant height, leaf width, number of flower and attitude of floral axis showed prevailing additive gene action while stem diameter, number of leaves, leaf length, days to flowering, flower diameter and bud length indicated predominance of dominance gene action. We can conclude that parent P5, parent P2 and parent P7 can be used as good mother lines and the tester 1, as good donor for the seed production. SAARC J. Agri., 17(1): 175-187 (2019)


2019 ◽  
Vol 1 (2) ◽  
pp. 143-152
Author(s):  
Redho Anggara Nubriama ◽  
Erwin Pane ◽  
Sumihar Hutapea

Cacao is one of the predominant plantation in Indonesia so that the production should be improved constantly. The purpose of this researce was to obtain data on the growth of cacao seedlings (Theobroma cacao L.) by applying rabbit cage liquid organic fertilizer and composting baglog waste with different doses. This research was carried out at the Medan Area faculty of agriculture experiment area located on the street PBSI No.1 Medan Estate. The research was conducted  from  July to  November  2018.  Using  Factorial  Randomized  Group Desing (RGD) with two replications. The first factor tested was rabbit cage liquid organic fertilizer namely U0= without POC, U1= consentration 5% (50 ml POC/liter), U2= consentration 10% (100 ml POC/liter), U3= consentration 15% (150 ml POC/liter). The second   factor is baglog waste compost which is L0= without compost baglog waste, L1= 20% baglog compost waste (600g) + 80% top soil (2.400g), L2= 40% baglog compost waste (1.200g) + 60% top soil (1.800g), L3= 60% baglog compost waste (1.800g) + 40% top soil (1.200g). The results showed that administration of baglog waste compost can increase growth ( plant height, number of leaves, stem diameter and canopy wet weight) of cocoa seedlings


Author(s):  
Patrícia Dos Santos Nascimento ◽  
Lucylia Suzart Alves ◽  
Vital Pedro da Silva Paz

This work evaluated the development of colored cotton submitted to irrigation water of different salinity levels and organic matter doses from tanned manure. The experimental design was completely randomized in a 4 x 4 factorial scheme with 3 replications, totaling 48 experimental plots. The factors studied were 4 doses of organic matter (1, 4, 7 and 10%) and four levels of irrigation water salinity (0.26; 1, 2 and 4 dS m-1). The variables analyzed were plant height, stem diameter, number of leaves, number of fruits and fresh shoot mass. Significant effect of organic matter was observed on all studied variables, with a positive response on the increase of all variables as a function of increasing doses of organic matter. The isolated effect of salinity was significant for all parameters evaluated, except for the number of leaves, with significant reductions of 6.03; 3.27; 5.23; 6.94% in the parameters: plant height, stem diameter, number of fruits and shoot fresh weight respectively, for each unit increase of irrigation water salinity. The interaction between the variation sources studied had a significant effect only for fresh shoot mass, where the highest average for this parameter was observed at 10% organic matter dosage at irrigation water salinity level of 1.0 dS m-1.


Author(s):  
D. N. Ogbonna ◽  
S. A. Ngah ◽  
R. N. Okparanma ◽  
O. Ule ◽  
R. R. Nrior

Aim: The aim of the study was to assess Percentage Bioremediation of Spent Mushroom Substrate (SMS) and Mucor racemosus in hydrocarbon contaminated soil Place and Duration of Study: A portion of Rivers State University demonstration farmland in Nkpolu-Oroworukwo, Mile 3 Diobu area of Port Harcourt, Rivers State was used for this study. The piece of land is situated at Longitude 4°48’18.50’’N and Latitude 6o58’39.12’’E measuring 5.4864 m x 5.1816 m with a total area of 28.4283 m2. Bioremediation monitoring lasted for 56 days, analysis carried out weekly (per 7 days’ interval). Methodology: Five (5) experimental plots employing the Randomized Block Design were used each having dimensions of 100 x 50 x 30 cm (Length x Breadth x Height) = 150,000cm3. Baseline study of the uncontaminated and the deliberately contaminated agricultural soil was investigated for its microbiota and physico-chemical properties. Two of these plots were designated as pristine (Unpolluted soil) (CTRL 1) and crude oil contaminated soil without nutrient organics and bioaugmenting microbes (CTRL 2); these two serve as controls. Each of the experimental plots, except the control (CTRL 1), was contaminated with 2500 cm3 (2122.25 g) of crude oil giving initial Total Petroleum Hydrocarbon (TPH) value of 8729.00 mg/kg. The crude oil polluted soil in Plot 3 was further treated with 750 ml of Mucor racemosus broth (CS+Muc), Plot 4 was treated with 3000 g of Spent Mushroom Substrate (CS+SMS) while plot 5 was treated with the combination of both (CS+Muc+SMS). The plots were left for 7 days to ensure even distribution and soil-oil bonding. Sampling was done at seven-day interval (Day 1, 7, 14, 21, 28, 35, 42, 49, 56).  Physicochemical parameters monitored were pH, Temperature, Nitrogen, Phosphorus, Potassium, and Total Petroleum Hydrocarbon (TPH) throughout the experimental period. Microbial parameters monitored were Total Heterotrophic Bacteria (THB), Total Heterotrophic Fungi (THF), Hydrocarbon Utilizing Bacteria (HUB) and Hydrocarbon Utilizing Fungi (HUF). Percentage (%) Bioremediation was estimated from percentage (%) reduction of Total Petroleum Hydrocarbon (TPH) from day 1 to day 56 in relation to control plots.  Net % Bioremediation were also assessed to ascertain the actual potential of treatment agents singly or combined. Results: Total Heterotrophic Bacteria (THB) (CFU/g) recorded on day 7 and day 56 of the bioremediation were; day 7; CTRL 1 – US (1.07 x109), CTRL- CS (5.4 x108), CS+Muc (3.0 x108), CS+SMS (4.6 x108) and CS+Muc+SMS (5.0 x108). On day 56, data obtained were CTRL 1 –US (9.4 x108), CTRL 2 –CS (7.2 x109), CS+Muc (3.7 x108), CS+SMS (8.1x108) and CS+Muc+SMS (6.8 x108). The increase in number in the treated plots is a depiction of an increase in activity of the organism and the stimulating effect of bio-organics SMS while the untreated plot CTRL 1-US showed decrease in population at day 56. Similar trend showed for Total Heterotrophic Fungi. Generally, it was observed that the highest growth/ count was recorded at the 7th and 8th week (day 42 or day 49), at the 9th week there was an observable decrease; probably due to depletion of nutrients and other factors such as rainfall and seepage. The Net Percentage Hydrocarbon Utilizing Bacteria and Fungi (Net %HUB and Net %HUF) were highest in Crude Oil contaminated plot treated with Spent Mushroom Substrate (SMS) singly; that is (CS+SMS) (11.02% and 12.07%) and lowest in the uncontaminated soil – Control (CTRL 1 –US) (5.41% and 9.26%) respectively. The trend in decreasing order of Net % Hydrocarbon Utilizing Bacteria were as follows: CS+SMS (11.02%) > CS+Muc+SMS (10.14%) > CS+Muc (9.43%) > CTRL 2 –CS (8.1%) > CTRL 1 –US (5.41%) while Net % Hydrocarbon Utilizing Fungi followed similar trend and were: CS+SMS (12.07%) > CS+Muc+SMS (11.76%) = CS+Muc (11.76%) > CTRL 2 –CS (11.05%) > CTRL 1 –US (9.26%). Evaluation of Amount of Crude Oil or Hydrocarbon remediated and Net %Bioremediation revealed Crude Oil contaminated plot augmented with Mucor racemosus broth singly (CS+Muc) as having the highest bioremediation potential while the least is the untreated soil. The trend is as follows:  CS+Muc (8599.19 mg/kg; 33.93%) > CS+Muc+SMS (8298.95 mg/kg; 32.74%) > CS+SMS (8197.03 mg/kg; 32.34%) > CTRL 2 –CS (166.54 mg/kg; 0.66%) > CTRL 1 –US (85.48 mg/kg; 0.34%) Conclusion: This shows that a single nutrient substrate or augmenting microorganism applied appropriately may have a more positive result, that is; higher bioremediation potential than combined or multiple mixed treatments. It was further observed that microbial counts decreased with time in treatments with augmenting organisms alone but increased considerably in treatments supplement with organics having its peak on the 49th day.   It is therefore recommended that bioremediation of crude oil-polluted soil using bio-augmenting microorganism should be applied appropriately noting the volume: area ratio and be supplemented with efficient nutrient organics after every 49-day interval.


HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 428d-428
Author(s):  
Darbie M. Granberry ◽  
William Terry Kelly

During summer in southern Georgia, greenhouses often reach or exceed 49C. Subsequently, transplants grown in these houses tend to exceed optimum height. To determine effects of N and P fertility levels on pepper (Capsicum annuum) transplant growth, applications of nutrient solutions with 58, 12, and 0 ppm N and 114, 57, and 0 ppm P205 were applied to recently emerged Capistrano bell pepper beginning 12 Aug. 1994. Plants were grown in polystyrene flats using a commercial noncharged artificial soil mix. Nutrient solutions were applied as needed to maintain adequate moisture until 31 Aug., except for two treatments that received only water after 22 Aug. Data were taken on 22 and 31 Aug. With 58 ppm N, 0 P decreased plant height, leaf count, leaf area, and plant weight. Higher N increased plant height, ratings of intact root plugs and washed roots, stem diameter, leaf count, leaf area, and plant weight. At 12 ppm N, P had no significant effect on transplant growth. Withholding fertilizer the final week reduced plant height, ratings of intact root plugs, stem diameter, number of leaves, leaf area, and plant weight.


2017 ◽  
Vol 8 (1) ◽  
pp. 29 ◽  
Author(s):  
Imanuel Jhon Hansen

The aim of research to determine the effect of compost main pod husks and dolomite as well as their interaction on the growth of cocoa seedlings in media ultisol. Research conducted at the Experimental Farm, Faculty of Agriculture, University of Riau from November 2015 until February 2016. Research in the form of factorial experiment using completely randomized design (CRD). The first factor pod husks compost comprises four levels (0, 75, 100 and 125 g / polybag) and the second factor dolomite consists of three levels (0, 10, and 20 g / polybag), each combination treatment was repeated 3 times. Parameters measured were plant height, leaf number, stem diameter, and leaf area. Each parameter the observed data were statistically analyzed using analysis of variance and test of Duncan's New Multiple Range Test (DNMRT) at 5% level. The results showed the effect of compost pod husks dose of 125 g / polybag significantly increased plant height, stem diameter, number of leaves and leaf area while dolomite dose of 20 g / polybag no real effect except leaf area compared with no treatment. Interaction pod husks compost dose of 100 g / polybag and dolomite dose of 20 g / polybag increase stem diameter, number of leaves and leaf area significantly compared without compost pod husks and dolomite but less tangible than other combinations.


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