scholarly journals Identification of an antimicrobial peptide from large freshwater snail (Lymnaea stagnalis): activity against antibiotics resistant Staphylococcus epidermidis

2016 ◽  
Vol 2 ◽  
pp. 5-9
Author(s):  
Samiran Sona Gauri ◽  
◽  
Chandan Kumar Bera ◽  
Rabindranath Bhattacharyya ◽  
Santi Mohan Mandal ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Staphylococcus Epidermidis ◽  
Lymnaea Stagnalis ◽  
Public Health Problem ◽  
Freshwater Snail ◽  
Reversed Phase ◽  
Bacterial Membranes ◽  
Wide Range ◽  
Phase Liquid ◽  
Conventional Antibiotics

Nowadays, antibiotic resistance in bacteria is a great public health problem of increasing magnitude due to quick evolution through mutation that has generated the urgency to find the effective solutions to address this problem. Aside the conventional antibiotics, antimicrobial peptides are a new class of antimicrobials is known to have the activity against a wide range of bacteria. An antimicrobial peptide was isolated and purified from the Lymnaea stagnalis, a fresh water large snail, using ultrafiltration and reversed phase liquid chromatography. The molecular mass of the peptide 2345 Da was determined using MALDI TOF mass spectrometry. This peptide is efficiently prevented the growth of Staphylococcus epidermidis that resistant to ampicillin and chloramphenicol antibiotics. The MIC value was 16 μg/mL and specifically damage to bacterial membranes. Hence, this reported peptide revealed an alternative candidate to controlling the Staphylococcal infections.

Measurement of urinary leukotrienes by reversed-phase liquid chromatography and radioimmunoassay.

1989 ◽  
Vol 35 (3) ◽  
pp. 388-391 ◽  
Author(s):  
P Tagari ◽  
D Ethier ◽  
M Carry ◽  
V Korley ◽  
S Charleson ◽  
...  
Keyword(s):  
Extraction Method ◽  
Reversed Phase ◽  
Cardiac Ischemia ◽  
Reversed Phase Hplc ◽  
Reversed Phase Liquid Chromatography ◽  
Sample Processing ◽  
Wide Range ◽  
Phase Liquid ◽  
Manual Extraction ◽  
Healthy Persons

Abstract Leukotriene (LT) E4, an important LT metabolite appearing in urine, can be rapidly separated from normal and pathological urines by automated reversed-phase HPLC after a simple sample-processing. The recoveries of LTE4 afforded by this system (86.4 +/- 6.5%, mean +/- SEM for 60 ng/L, 85.4 +/- 0.3% for 200 ng/L) are superior to those obtained by a manual extraction method. Consistency of results is similar. Highly reproducible retention times combined with a radioimmunoassay allow one to identify (based on co-elution) and quantify as little as 8 ng/L LTE4 in a 10-mL urine sample. LTE4 concentrations in urine from healthy persons approach this value (17 +/- 5 ng/L), whereas samples from patients with cardiac ischemia show a wider range of concentrations (8 to 388 ng/L), up to 50 times the detection limit. Thus this method is applicable to the noninvasive investigation of leukotriene involvement in a wide range of ischemic, inflammatory, and hypersensitive conditions.

Triply-periodic nanostructures in surfactant, block copolymer, and biomembrane systems, and simulation of TEMs

1993 ◽  
Vol 51 ◽  
pp. 884-885
Author(s):  
David M. Anderson ◽  
Tomas Landh
Keyword(s):  
Liquid Crystalline ◽  
Diblock Copolymers ◽  
Cubic Phase ◽  
List Type ◽  
Interpenetrating Networks ◽  
Triply Periodic ◽  
Wide Range ◽  
Bicontinuous Cubic ◽  
Phase Liquid ◽  
Dividing Surface

First discovered in surfactant-water liquid crystalline systems, so-called ‘bicontinuous cubic phases’ have the property that hydropnilic and lipophilic microdomains form interpenetrating networks conforming to cubic lattices on the scale of nanometers. Later these same structures were found in star diblock copolymers, where the simultaneous continuity of elastomeric and glassy domains gives rise to unique physical properties. Today it is well-established that the symmetry and topology of such a morphology are accurately described by one of several triply-periodic minimal surfaces, and that the interface between hydrophilic and hydrophobic, or immiscible polymer, domains is described by a triply-periodic surface of constant, nonzero mean curvature. One example of such a dividing surface is shown in figure 5.The study of these structures has become of increasing importance in the past five years for two reasons:1)Bicontinuous cubic phase liquid crystals are now being polymerized to create microporous materials with monodispersed pores and readily functionalizable porewalls; figure 3 shows a TEM from a polymerized surfactant / methylmethacrylate / water cubic phase; and2)Compelling evidence has been found that these same morphologies describe biomembrane systems in a wide range of cells.

Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography

2013 ◽  
Vol 41 (2) ◽  
pp. 181
Author(s):  
Ling DING ◽  
Jun DONG ◽  
Yuan-Sheng XIAO ◽  
Xiu-Li ZHANG ◽  
Xing-Ya XUE ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Retention Equation ◽  
Phase Liquid
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