In vitro Explant Growth and Shoot Regeneration from Petioles of Sugar Beet (Beta vulgaris L.) Lines at Different Ploidy Levels

2013 ◽  
Vol 50 (1) ◽  
pp. 22-36
Author(s):  
Mustafa Yildiz ◽  
Behrouz Alizadeh ◽  
Ramazan Beyaz
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Shoot Regeneration ◽  
Ploidy Levels

Callus Induction and Shoot Organogenesis in Two Sugar Beet (Beta vulgaris L.) Breeding Lines in vitro Cultured

2013 ◽  
Vol 12 (4) ◽  
pp. 168-178 ◽  
Author(s):  
Farhad Taghipour ◽  
Narges Janalizade ◽  
Maryam Eshrati ◽  
Taraneh Hassanzade ◽  
Fahrul Huyop
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Callus Induction ◽  
Shoot Organogenesis ◽  
Breeding Lines

scholarly journals Methods of creating homozygous lines as breeding genotypes in sugar beet (Beta vulgaris) with apozygotic method of seed reproduction

Bioenergy ◽  
2021 ◽  
Author(s):  
M. V. Roik ◽  
N. S. Kovalchuk ◽  
O. A. Zinchenko ◽  
L. H. Fedoroshchak ◽  
V. I. Vlasiuk ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Sugar Beets ◽  
Alloplasmic Lines ◽  
Total Percentage ◽  
A Value ◽  
Open Ground ◽  
Genome Ploidy ◽  
Selection Of

Purpose. Investigation of cytogenetic aspects of embryological processes in the culture of immature apomictic embryos, breeding genotypes of sugar beet with cytoplasmic sterility for differentiation and selection by gametophyte reduced parthenogenesis. Methods. Cytological, biotechnological, fluorescent cytophotometry, field, laboratory. Results. The cytogenetic features of genesis of immature apomictic embryos cells induced in vitro on the 12th, 20th and 22th days of development have been investigated on the basis of CMS apozygotic lines of Beta vulgaris and alloplasmic lines of wild species Beta maritime and Beta patula. Indicators of efficiency of haploid reduced parthenogenesis in vitro in alloplasmic lines significantly exceeded the best technologies in pollen-sterile lines of sugar beet from 3.79% to 6.25% and had a value of 62.2%, 24.8%, and 16.7%, respectively. Stabilization of genome ploidy to diploid was carried out in selected breeding numbers without colchicine, based on evaluation and selection of genome ploidy using software of ploidy analyzer (AP) Partec. Conclusions. The efficiency of haploid reduced parthenogenesis induction in vitro in apozygotic CMS breeding genotypes of sugar beet as affected by genetic potential of cytoplasm and taking into account the total percentage of haploids (50 units; 100 units) and myxoploids (50 units; 100 units) has been investigated. Homozygous lines were created by stabilizing the genome ploidy of haploid and myxoploid micro sprouts during III–IV passages without the use of colchicine. Technologies of rooting in the open ground for use in the breeding process of sugar beets have been improved.

Cryopreservation of in vitro sugar beet ( Beta vulgaris L.) shoot tips by a vitrification technique

2000 ◽  
Vol 19 (11) ◽  
pp. 1064-1068 ◽  
Author(s):  
B. Vandenbussche ◽  
G. Weyens ◽  
M. De Proft
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Shoot Tips
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