Addition of a thermostabilized pectin methylesterase significantly enhances the rate of saccharification of sugar beet pulp preparation.

2009 ◽  
Author(s):  
Robert Kozak ◽  
Craig S. Laufer
Keyword(s):  
Sugar Beet ◽  
Pectin Methylesterase ◽  
Sugar Beet Pulp ◽  
Beet Pulp

scholarly journals Thermal Stabilization of Erwinia chrysanthemi Pectin Methylesterase A for Application in a Sugar Beet Pulp Biorefinery

2009 ◽  
Vol 75 (23) ◽  
pp. 7343-7349 ◽  
Author(s):  
Chacko Chakiath ◽  
Margaret J. Lyons ◽  
Robert E. Kozak ◽  
Craig S. Laufer
Keyword(s):  
Sugar Beet ◽  
Specific Activity ◽  
Pectin Methylesterase ◽  
Significant Loss ◽  
Sugar Beet Pulp ◽  
Erwinia Chrysanthemi ◽  
Kinetic Properties ◽  
Wild Type ◽  
Wild Type Enzyme ◽  
Beet Pulp

ABSTRACT Directed evolution approaches were used to construct a thermally stabilized variant of Erwinia chrysanthemi pectin methylesterase A. The final evolved enzyme has four amino acid substitutions that together confer a Tm value that is approximately 11°C greater than that of the wild-type enzyme, while maintaining near-wild-type kinetic properties. The specific activity, with saturating substrate, of the thermally stabilized enzyme is greater than that of the wild-type enzyme when both are operating at their respective optimal temperatures, 60°C and 50°C. The engineered enzyme may be useful for saccharification of biomass, such as sugar beet pulp, with relatively high pectin content. In particular, the engineered enzyme is able to function in biomass up to temperatures of 65°C without significant loss of activity. Specifically, the thermally stabilized enzyme facilitates the saccharification of sugar beet pulp by the commercial pectinase preparation Pectinex Ultra SPL. Added pectin methylesterase increases the initial rate of sugar production by approximately 50%.

Obtaining of sorbing material based on sugar beet pulp

2019 ◽  
pp. 10-16
Author(s):  
S.V. Meshcheryakov ◽  
◽  
I.S. Eremin ◽  
D.O. Sidorenko ◽  
M.S. Kotelev ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Sugar Beet Pulp ◽  
Beet Pulp

Kinetic and thermodynamic study of the adsorption of calcium onto sugar beet pulp

2016 ◽  
pp. 565-570
Author(s):  
Huang Qin ◽  
Zhu Si-ming ◽  
Zeng Di ◽  
Yu Shu-juan
Keyword(s):  
Sugar Beet ◽  
Adsorption Equilibrium ◽  
Ph Value ◽  
Hard Water ◽  
Sugar Beet Pulp ◽  
Order Kinetic ◽  
Factors Affecting ◽  
Isotherm Equation ◽  
Equilibrium Data ◽  
Beet Pulp

Sugar beet pulp (SBP) was used as low value adsorbent for the removal of calcium from hard water. Batch experiments were conducted to determine the factors affecting adsorption of the process such as pH value and Ca concentration. The adsorption equilibrium of Ca2+ by the SBP is reached after 100min and a pseudo second-order kinetic model can describe the adsorption process. The initial concentrations of Ca varied from 927 to 1127mgCa2+/L. A dose of 30g/L sugar beet pulp was sufficient for the optimum removal of calcium. The overall uptake of Ca ions by sugar beet pulp has its maximum at pH=8. The adsorption equilibrium data fitted well with the Langmuir adsorption isotherm equation.

Possibilities of joint treatment of spent sugar beet pulp and waste water from a sugar factory

2012 ◽  
pp. 756-761 ◽  
Author(s):  
Miroslav Hutnan ◽  
Štefan Tóth ◽  
Igor Bodík ◽  
Nina Kolesárová ◽  
Michal Lazor ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Sugar Beet ◽  
Pilot Plant ◽  
Biogas Production ◽  
Sugar Beet Pulp ◽  
Spare Capacity ◽  
Anaerobic Reactor ◽  
Sugar Factory ◽  
Beet Pulp ◽  
Joint Treatment

The possibility of joint treatment of spent sugar beet pulp and wastewater from a sugar factory was studied in this work. Works focused on processing of spent sugar beet pulp separately or together with other substrates can be found in the literature. In the case of some sugar factories, which have spare capacity in the anaerobic reactor on an anaerobic-aerobic wastewater treatment plant, joint processing of spent sugar beet pulp and wastewater from the sugar factory might be an interesting option. The results of the operation of a pilot plant of an anaerobic reactor with a capacity of 3.5 m3 are discussed. Operation of the pilot plant confirmed the possibility of cofermentation of these materials. The organic loading rate achieved in the anaerobic reactor was higher than 6 kg/(m3·d) (COD), while more than half of the load was provided by spent sugar beet pulp. The addition of sugar beet pulp decreased the concentration of ammonia nitrogen in the anaerobic reactor and it was even necessary to add nitrogen. However, the nitrogen content in sludge water depends on the C:N ratio in the processed sugar beet pulp, therefore this knowledge cannot be generalized. About 1.5 to 2-fold biogas production can be expected from the cofermentation of wastewater with sugar beet pulp in an anaerobic reactor, compared with the biogas production from just wastewater treatment.

Pomelo peel and sugar beet pulp as novel biosorbents in purification of biodiesel

Biofuels ◽  
2021 ◽  
pp. 1-8
Author(s):  
Saida Ibragić ◽  
Narcisa Smječanin ◽  
Ranko Milušić ◽  
Mirza Nuhanović
Keyword(s):  
Sugar Beet ◽  
Sugar Beet Pulp ◽  
Pomelo Peel ◽  
Beet Pulp

scholarly journals The Influence of Extruded Sugar Beet Pulp on Cookies' Nutritional, Physical and Sensory Characteristics

2021 ◽  
Vol 13 (9) ◽  
pp. 5317
Author(s):  
Sonja Simić ◽  
Jovana Petrović ◽  
Dušan Rakić ◽  
Biljana Pajin ◽  
Ivana Lončarević ◽  
...  
Keyword(s):  
Particle Size ◽  
Sugar Beet ◽  
Dietary Fiber ◽  
Wheat Flour ◽  
Nutritional Quality ◽  
Sugar Beet Pulp ◽  
Sensory Characteristics ◽  
Total Dietary Fiber ◽  
Beet Pulp

Sugar beet pulp (SBP) is a by-product of the sugar industry in which the dietary fiber content ranges from 73% to 80%. Compared to cereal fibers mainly used in biscuit production, sugar beet fibers are gluten free and have a perfect ratio of 2/3 insoluble fiber. In this work, sugar beet pulp was extruded with corn grits (ratios of corn grits to sugar beet pulp in extrudates were 85:15, 70:30, and 55:45), and the obtained sugar beet pulp extrudates (SBPEs) were used for improving the nutritional quality of cookies. The wheat flour in cookies was replaced with SBPEs in the amount of 5, 10, and 15%. The influence of three factors (the percentage of sugar beet pulp in the SBPEs, the size of the SBPE particles, and the percentage of wheat flour substituted with SBPEs) and their interactions on the nutritional quality of cookies, as well as their physical and sensory characteristics are examined using the Box–Behnken experimental design. The addition of extruded sugar beet pulp (SBPEs) significantly increased the amount of total dietary fiber and mineral matter of cookies. On the whole, the addition of SBPEs increased cookie hardness, but the hardness decreased with an increase in extrudate particle size. Sensory characteristics (except for the taste) were the most influenced by extrudate particle size.

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