scholarly journals Biochemical composition of Microcystis aeruginosa related to specific growth rate: insight into the effects of abiotic factors

Inland Waters ◽  
2014 ◽  
Vol 4 (4) ◽  
pp. 357-362 ◽  
Author(s):  
Ming Li ◽  
Philip Nkrumah ◽  
Man Xiao
2001 ◽  
Vol 67 (1) ◽  
pp. 278-283 ◽  
Author(s):  
Benedict M. Long ◽  
Gary J. Jones ◽  
Philip T. Orr

ABSTRACT Cell quotas of microcystin (Q MCYST; femtomoles of MCYST per cell), protein, and chlorophyll a(Chl a), cell dry weight, and cell volume were measured over a range of growth rates in N-limited chemostat cultures of the toxic cyanobacterium Microcystis aeruginosa MASH 01-A19. There was a positive linear relationship betweenQ MCYST and specific growth rate (μ), from which we propose a generalized model that enablesQ MCYST at any nutrient-limited growth rate to be predicted based on a single batch culture experiment. The model predicts Q MCYST from μ, μmax(maximum specific growth rate), Q MCYSTmax(maximum cell quota), and Q MCYSTmin (minimum cell quota). Under the conditions examined in this study, we predict aQ MCYSTmax of 0.129 fmol cell−1 at μmax and a Q MCYSTmin of 0.050 fmol cell−1 at μ = 0. Net MCYST production rate (R MCYST) asymptotes to zero at μ = 0 and reaches a maximum of 0.155 fmol cell−1 day−1at μmax. MCYST/dry weight ratio (milligrams per gram [dry weight]) increased linearly with μ, whereas the MCYST/protein ratio reached a maximum at intermediate μ. In contrast, the MCYST/Chla ratio remained constant. Cell volume correlated negatively with μ, leading to an increase in intracellular MCYST concentration at high μ. Taken together, our results show that fast-growing cells of N-limited M. aeruginosa are smaller, are of lower mass, and have a higher intracellular MCYST quota and concentration than slow-growing cells. The data also highlight the importance of determining cell MCYST quotas, as potentially confusing interpretations can arise from determining MCYST content as a ratio to other cell components.


2016 ◽  
Vol 15 (2) ◽  
pp. 171 ◽  
Author(s):  
Yuni Puji Hastuti ◽  
Horas Nadeak ◽  
Ridwan Affandi ◽  
Kurnia Faturrohman

<p class="NoParagraphStyle"><strong>ABSTRACT</strong><strong></strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">One of the abiotic factors that affects the growth and the survival of crabs is pH. The optimum pH media will give maximum impact on mangrove crabs because it is related with the osmoregulation process. This study aimed to examine the effect of pH on the survival rate (SR) and specific growth rate (SGR) of mangrove crab <em>Scylla serrata</em> through the reaction of physiological condition. This study consisted of the treatments with the pH medium 5 (A), pH medium 6 (B), pH medium 7 (C), and pH medium 8 (D).  The crab’s maintenance in different pH gave a significant effect (P&lt;0.05) on the survival rate of the crabs. The pH treatments also gave a significant effect (P&lt;0.05) on the SGR of the crabs. The low level of crab stress at pH 7 was described by  the total value of high hemocyte and the high osmotic load so that the pH 7 was the optimum condition for the crabs.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: pH, survival, specific growth rate, mangrove crabs</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Salah satu faktor abiotik yang mempengaruhi pertumbuhan dan kelangsungan hidup kepiting adalah pH. Media pH optimum akan memberikan dampak maksimum pada kepiting bakau karena terkait dengan proses osmoregulasi. Penelitian ini bertujuan untuk menguji pengaruh pH pada tingkat kelangsungan hidup (SR) dan laju pertumbuhan spesifik (SGR) kepiting mangrove <em>Scylla serrata</em> melalui reaksi kondisi fisiologis. Penelitian ini terdiri atas perlakuan dengan media pH 5 (A), sedang pH 6 (B), sedang pH 7 (C), dan menengah pH 8 (D). Pemeliharaan kepiting di pH yang berbeda memberikan pengaruh yang signifikan (P&lt;0,05) pada tingkat kelangsungan hidup kepiting. Perlakuan pH juga memberikan efek yang signifikan (P&lt;0,05) pada SGR dari kepiting. Rendahnya tingkat stres kepiting pada pH 7 digambarkan oleh nilai total hemosit tinggi dan beban osmotik tinggi sehingga pH 7 adalah kondisi optimum untuk kepiting.</p><p class="NoParagraphStyle"> </p><p>Kata kunci: pH, kelangsungan hidup, laju pertumbuhan spesifik, kepiting mangrove</p>


2014 ◽  
Vol 535 ◽  
pp. 577-580
Author(s):  
Qing Man Cui ◽  
Hai Wen Wang ◽  
Ping Liu ◽  
Chun Ying Yuan

The effect of salinity (10,20,30,40,50) on the growth and biochemical composition of Sargassum thunbergii and Gracilaria verrucosa were investigated under the laboratory conditions, the results showed that: specific growth rate and chlorophyll a of Sargassum thunbergii and Gracilaria verrucosa were first increased and then decreased with the increase of salinity, the carotenoids content of Sargassum thunbergii in 10 salinity group and Gracilaria verrucosa in 40 salinity group were the maximum respectively. After comprehensive analysis, in the 10-50 salinity range, The optimal salinity for the growth of G.verrucosa and S. thunbergii were 20 and 30 respectively.


2015 ◽  
Vol 14 (1) ◽  
pp. 50 ◽  
Author(s):  
Yuni Puji Hastuti ◽  
Ridwan Affandi ◽  
Mafatih Devi Safrina ◽  
Kurnia Faturrohman ◽  
Wildan Nurussalam

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"> </p><p class="NoParagraphStyle">One of the abiotic factors that affects the growth and the survival of crabs is salinity. The optimum salinity media will give maximum impact on mangrove crab <em>Scylla serrata</em> due to the osmoregulation process. This study aimed to examine the effect of salinity on the survival rate (SR) and spesific growth rate (SGR) of mangrove crab through the reaction of physiological condition. The treatments were rearing mangrove crab at the salinity medium of 15 ppt (A), 20 ppt (B), 25 ppt (C), and 30 ppt (D). Result showed that different salinity performed a significant effect (P&lt;0.05) on the survival rate and specific growth rate of the crabs. The low level of stress, shown by the high value of total hemocyte and the low osmotic pressure, has made salinity of 25 ppt was the optimum condition for the mangrove crab rearing.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: salinity, survival, specific growth rate, mangrove crab</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Salah satu faktor abiotik yang memengaruhi pertumbuhan dan kelangsungan hidup kepiting adalah salinitas. Salinitas media optimum akan memberikan efek yang maksimal pada kepiting bakau <em>Scylla serrata</em> sehubungan dengan proses osmoregulasi tubuhnya. Penelitian ini bertujuan untuk menguji pengaruh salinitas pada kelangsungan hidup dan laju pertumbuhan spesifik kepiting bakau melalui reaksi kondisi fisiologis. Penelitian ini terdiri atas perlakuan salinitas media 15 ppt (A), 20 ppt (B), 25 ppt (C), dan 30 ppt (D). Hasil penelitian menunjukkan bahwa perbedaan salinitas media pemeliharaan kepiting bakau memberikan perbedaan nyata (P&lt;0,05) pada kelangsungan hidup dan laju pertumbuhan spesifik kepiting bakau. Rendahnya tingkat stres pada salinitas 25 ppt dijelaskan dengan tingginya jumlah total hemosit dan rendahnya tekanan osmotik sehingga salinitas 25 ppt merupakan kondisi optimum bagi pemeliharaan kepiting bakau.</p><p class="NoParagraphStyle"> </p><p>Keywords: salinitas, kelangsungan hidup, laju pertumbuhan spesifik, kepiting bakau</p>


Aquaculture ◽  
2008 ◽  
Vol 274 (1) ◽  
pp. 87-95 ◽  
Author(s):  
S. Millot ◽  
M.-L. Bégout ◽  
J. Person-Le Ruyet ◽  
G. Breuil ◽  
C. Di-Poï ◽  
...  

2010 ◽  
Vol 45 (11) ◽  
pp. 1800-1807 ◽  
Author(s):  
S. Chenikher ◽  
J.S. Guez ◽  
F. Coutte ◽  
M. Pekpe ◽  
P. Jacques ◽  
...  

2012 ◽  
Vol 48 (2) ◽  
pp. 382-386 ◽  
Author(s):  
Juan Aguirre ◽  
Mª Rosa Rodríguez ◽  
Rodrigo González ◽  
Gonzalo García de Fernando

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