Evaluation of Antibacterial Potential of New Acid Dyes Based on Substituted Aryl Amines and Amino Hydroxy Sulfonic Acid

2020 ◽  
Vol 42 (5) ◽  
pp. 783-783
Author(s):  
Zeeshan Akhtar Zeeshan Akhtar ◽  
Syed Imran Ali Syed Imran Ali ◽  
Naseem Abbas Naseem Abbas ◽  
Muhammad Ali Muhammad Ali ◽  
Muhammad Yasir Khan Muhammad Yasir Khan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Sulfonic Acid ◽  
Diffusion Method ◽  
Dye Molecules ◽  
Bacterial Strains ◽  
Acid Dyes ◽  
Aryl Amines ◽  
Coupling Components ◽  
Antibacterial Potential

Six newly synthesized acid dye derivatives were evaluated for their in vitro antimicrobial activity against four (04) Gram positive (Staphylococcus aureus, Staphylococcus lugdunensis, Streptococcus pneumoniae and Bacillus subtilis) and six (06) Gram negative (Pseudomonas aeruginosa, Acinetobacter baumannii, Citrobacter freundii, Klebsiella pneumoniae,Pseudomonas fluorescens and Shigella sonnei) bacterial strains. The dyes were synthesized using substituted aryl amines and substituted amino hydroxy sulfonic acid as diazo and coupling components, respectively. The antimicrobial activity was explored by Agar-Well Diffusion method using 100 mg/ml dye concentration. The results demonstrated that the dyes possess varying degrees of activities against the bacterial strains tested and the structure of the dye molecules was found to have considerable influence on their antimicrobial activity.

scholarly journals Phytochemical investigation and in vitro antimicrobial activity of Richardia scabra

2016 ◽  
Vol 11 (2) ◽  
pp. 248 ◽  
Author(s):  
Kathirvel Poonkodi ◽  
Subban Ravi
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Weed Species ◽  
Antibacterial And Antifungal Activities ◽  
Agar Plug ◽  
Phytochemical Investigation ◽  
Antibacterial And Antifungal ◽  
Simple Sugar

<p class="Abstract">The present study was aimed to evaluate the phytochemical screening and antimicrobial activity of the petroleum ether and methanol extracts from the mature leaves of <em>Richardia scabra</em> from India. Disc diffusion method was used to determine the zone inhibition of the tested samples for antibacterial and agar plug method was used to determine the antifungal activity, while the microtube-dilution technique was used to determine the minimum inhibitory concentration. Both extracts showed significant antibacterial and antifungal activities when tested against 10 bacterial and four fungal strains. The minimum inhibitory concentrations of the methanol extract of<em> R. scabra</em> ranged between 12.5–100 μg/mL for bacterial strains. Alkaloids, steroids, flavonoids, fatty acids, terpenoids and simple sugar were detected as phytoconstituents of extracts. To the best of our knowledge, this is the first report against antimicrobial activity of common weed species <em>R. scabra</em> found in India.</p><p> </p>

scholarly journals A Comparative Study of Antimicrobial Activity of Ethanolic Extract of Tecoma stans and Abutilon indicum Leaves: An In vitro Study

2021 ◽  
pp. 609-615
Author(s):  
M. Binigha ◽  
R. Gayatri Devi ◽  
J. Selavaraj ◽  
A. Jothi Priya
Keyword(s):  
Antimicrobial Activity ◽  
Agar Plate ◽  
Ethanolic Extract ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
The Family ◽  
Abutilon Indicum

Tecoma stans is a flowering plant belonging to the family Bignoniaceae. It is the floral emblem of the Bahamas. Abutilon indicum belongs to the family Malvaceae. It is also called Indian Mallow. Tecoma stans and Abutilon indicumhave antimicrobial activity and can be used in producing antibiotics. There is an urgent need to produce new antibiotics as there is an increase in the development of pathogen resistant drugs. The aim of this study was to compare the antimicrobial activity of ethanolic extract of Tecoma stansand Abutilonindicum leaves. E.faecalis bacteria were maintained in nutrient agar slopes. The powder of the two herbs, were subjected to extraction by Soxhlet extractor and was concentrated to dry residue by Agar well diffusion method. The nutrient broth was inoculated with bacterial strains. The culture was adjusted to 0.5McFarland turbidity standard. Lawn culture was done in Muller-Hinton agar plate and then the plates were dried. A 6 mm diameter well is bored for different concentrations. The extracts were introduced into the well and allowed to stand for 24 hrs. The antibacterial activity was determined by measuring the zone of inhibition. The present finding on the antimicrobial potential of Abutilon indicum and Tecoma stands showed that the two plants have growth inhibitory activity against oral pathogen E. faecalis. Abutilon indicum has a zone of inhibition greater than Tecoma stans. Abutilon indicum exhibited better activity compared to Tecoma stans.

Heteroaromatization with 4-Phenyldiazenyl-1-naphthol. Part IV: Synthesis of Some New Heterocyclic Compounds with Potential Biological Activity

2019 ◽  
Vol 16 (6) ◽  
pp. 931-938
Author(s):  
Hany M. Mohamed ◽  
Ashraf H.F. Abd El-Wahab
Keyword(s):  
Antimicrobial Activity ◽  
Agar Medium ◽  
Potassium Hydroxide Solution ◽  
Antimicrobial Activities ◽  
Azo Compounds ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Pharmacological Agents ◽  
Tetrazole Derivatives

Background: Synthetic azo compounds and their derivatives have been studied extensively due to their biological and pharmacological activities. Pyranopyridines, pyranopyrimidines and tetrazoles derivatives have emerged as a promising and attractive scaffold in the development of potent biological and pharmacological agents. Objectives: To design a series of new benzochromeno(pyridine/pyrimidine/tetrazole) derivatives and evaluate their antimicrobial activity against some bacterial strains (Gram-positive and Gram-negative) and some fungal strains. Materials and Methods: The (E)-7-(4-chlorophenyl)-5-(phenyldiazenyl)-10-thioxo-7,9,10,11-tetrahydro-8Hnaptho[ 1,2-b]pyrano[2,3-d]pyrimidin-8-one (4) was synthesized by the reaction of 4H-naphtho[1,2-b]pyran-3- carbonitrile (3) with carbon disulfide in alcoholic potassium hydroxide solution. Reaction of 3 with sodium azide in DMF and in presence of ammonium chloride afforded 6-(phenyldiazenyl)-3-(1H-tetrazol-5-yl)-4Hbenzo[ h]chromen-2-amine (7) while with malononitrile, thiourea or urea gave chromeno[2,3-b]pyridine-9- carbonitrile (8), chromeno[2,3-d]pyrimidine-10-thione (9) and chromeno[2,3-d]pyrimidin-10-one (10), respectively. The assignment structures were established on the basis of spectral data. Results: In this study, the antimicrobial activity of the synthesized compounds 3-12 was examined for their in vitro antimicrobial activity by using agar diffusion method such as Mueller-Hinton agar medium for bacteria and Sabouraud’s agar medium for fungi. Ampicillin and mycostatine were included in the experiments as reference drugs. Conclusion: A series of new benzochromeno(pyridine/pyrimidine/tetrazole) derivatives were synthesized in this work. All compounds were evaluated in antimicrobial activities.

scholarly journals Evaluation of Antimicrobial Activity of Extracts of Terminalia chebula and Piper nigrum against Streptococcus mutans: An In Vitro Study

2021 ◽  
pp. 218-224
Author(s):  
Pavani Bellamkonda ◽  
Ramesh Kumar Koothati ◽  
Aamina Bee ◽  
Abhishree Desai ◽  
G. K. Aarthi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibiotic Resistance ◽  
In Vitro Study ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Piper Nigrum ◽  
Terminalia Chebula ◽  
Bacterial Strains ◽  
Antimicrobial Potency

Objective: Dental caries is an infectious disease in which S. mutans plays a key role. Haphazard and irrational use of antibiotics leads to antibiotic resistance and fatal diarrhoeal diseases in children. Antimicrobial potency of Terminalia chebula and Piper nigrum extracts against several bacterial strains have been documented. The aims of this study were to assess and compare the antimicrobial activities of T. chebula and P. nigrum extracts against S. mutans with Ciprofloxacin as the positive control. Materials and Methods: For this purpose, S. mutans was isolated from plaque samples of people with active caries lesions.  Antimicrobial potency of both T. chebula and P.nigrum were tested using agar well diffusion method. Results: All the tested extracts showed antibacterial activity against S. mutans bacteria. Regarding the two tested herbs extracts, a higher antimicrobial activity was shown by the methanol extract of T. chebula with a mean diameter of inhibition zone was 26.75mm and a minimum inhibitory concentration (MIC) at 25mg /ml concentration followed by acetonic extract. Conclusion: These findings confirm the Antimicrobial potency of T. chebula which can be used as an alternative antibiotic and/or in combination with allopathic antibiotics to prevent the antibiotic resistance.

In vitro Evaluation of the Antimicrobial activity of Methanolic and Aqueous extract of Mucuna pruriens seed

2021 ◽  
pp. 4212-4214
Author(s):  
Amita Shobha Rao ◽  
Shobha K L ◽  
Preethi Lavina Concessao ◽  
Gowrish Rao S ◽  
Sreedhara R Pai K
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Mucuna Pruriens ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Clinical Strains

The present investigation was to find out efficacy of Mucuna pruriens (M. pruriens) seeds. The antimicrobial activity of methanol and aqueous extract of M. pruriens seeds was determined by well diffusion method with ATCC strains of gram positive and gram negative bacteria clinical strains of multidrug resistant (MDR) Klebsiella pneumoniae, and clinical strains of Candida albicans. It was observed that both the aqueous and the methanol extract of M. pruriens seeds had antibacterial activity against all the ATCC bacterial strains. The MDR strain of Klebsiella pneumoniae was resistant to both aqueous and methanol extract of M. pruriens seed. Antifungal activity against Candida albicans was exhibited by both aqueous and methanol extracts of M. pruriens seed.

scholarly journals In vitro study of antimicrobial activity of some plant seeds against bacterial strains causing food poisoning diseases

2022 ◽  
Vol 82 ◽  
Author(s):  
A. A. Abu-Zaid ◽  
A. Al-Barty ◽  
K. Morsy ◽  
H. Hamdi
Keyword(s):  
Antimicrobial Activity ◽  
Nigella Sativa ◽  
Food Poisoning ◽  
In Vitro Study ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Plant Seeds

Abstract In this research, some plant seeds powder was evaluated to find their potential effect to rule diseases of food poisoning. Antimicrobial effect of five plant seeds was examined contra Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella. pneumonia and Candida albicans by using well diffusion method. Antimicrobial activity studies revealed high potential activity of plant seeds powder of Nigella sativa L., cucurbita pepo, Sesamum radiatum, Trigonella foenum-graecum, Linum usitatissimum with variable efficiency contra tested microbial strains with concentration of 100 mg/ml, except Sesamum radiatum scored no effect. The T. foenum and N. sativa seed powder showed the largest inhibition zone (24-20 mm) contra K. pneumonia, followed by S. aureus (20-18 mm) and C. albicans (15mm) respectively. The five plant seeds powder exhibited bacteriostatic and bactericidal effects with MIC’s 20 and MBC 40 mg/ml against K. pneumonia, and MIC’s 40 and MBC 60 mg/ml against S. aureus. The results of this study indicated that plants seeds powder have promising antimicrobial activities and their potential applications in food process. It could be utilized as a natural medicinal alternative instead of chemical substance.

scholarly journals Novel Triazine Centred Manganese Based Complex: A Photophysical and Biological Studies

2019 ◽  
Vol 32 (1) ◽  
pp. 91-94
Author(s):  
L. Alphonse ◽  
P. Tharmaraj ◽  
B. Avila Josephine ◽  
V. Mary Teresita
Keyword(s):  
Antimicrobial Activity ◽  
Metal Complex ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Fluorescent Properties ◽  
E Coli ◽  
Physico Chemical ◽  
Biological Studies ◽  
Ft Ir

In this work, Mn(II) complex of triazine based ligand has been synthesized and characterized using various physico-chemical methods including C,H,N elemental analysis, FT-IR, 1H NMR and EI-mass analysis. The synthesized compounds serve as potential photoactive material as indicated from its characteristic fluorescent properties. The ligand and its metal complex were assayed for in vitro antimicrobial activity on four bacterial strains (S. aureus, B. subtilis, E. coli and K. pneumoniae) using well-diffusion method and it was observed that metal complex showed enhanced antimicrobial activity against all tested strains as compared to ligand.

scholarly journals Antibacterial Activity of Bees Gut Lactobacilli against Paenibacillus Larvae In Vitro

2018 ◽  
Vol 2 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Miroslava Kačániová ◽  
Jaroslav Gasper ◽  
Margarita Terentjeva ◽  
Simona Kunová ◽  
Maciej Kluz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Activities ◽  
Paenibacillus Larvae ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
De Man ◽  
Lactobacillus Spp

Abstract The aim of this study was to evaluate antimicrobial activity of bees gastrointestinal Lactobacillus spp. of against Paenibacillus larvae. Content of the intestinal tract was cultured for isolation of Lactobacillus spp. Gut homogenates were plated on de Man, Rogosa and Sharpe agar (MRS, Oxoid) plates and incubated for 48-72h at 30°C anaerobically. Then, the identification of isolates with MALDI-TOF MS Biotyper was done. The bacterial strains Lactobacillus gasseri, L. amylovorus, L. kunkeei, L. fructivorans, Paenibacillus larvae were isolated from gut content of bees. The disc diffusion method was used for the determination of antimicrobial activities of the Lactobacillus supernatant against two strains of Paenibacillus larvae. The best antimicrobial activity of Lactobacillus against Paenibacillus larvae from gut was found in L. gasseri supernatant. Lesser degree of antimicrobial activity against P. larvae was found in L. kunkeei supernatant. The strongest antibacterial activity against P. larvae CCM 4438 was found in L. gasseri and L. amylovorus and the least antibacterial activity was found in L. fructivorans.

Preliminary in Vitro Investigations on The Inhibitory Activity of The Original Dietary Supplement Oxidal® on Pathogenic Bacterial Strains

2020 ◽  
Vol 11 ◽  
pp. 37-43
Author(s):  
Prof. Teodora P. Popova ◽  
Toshka Petrova ◽  
Ignat Ignatov ◽  
Stoil Karadzhov
Keyword(s):  
Dietary Supplement ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Clinical Effectiveness ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Microbial Strains

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

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