scholarly journals Research progress of platelet-rich plasma in the treatment of androgenetic alopecia

2019 ◽  
Vol 3 (1) ◽  
pp. 9-14
Author(s):  
Ying Zhao ◽  
Lei Chen ◽  
Dongyu Yang ◽  
Yu Zheng
Keyword(s):  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Androgenetic Alopecia ◽  
Research Progress ◽  
Future Application ◽  
High Concentration ◽  
Treatment Progress ◽  
Promote Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Platelet-rich plasma (PRP) is a high-concentration platelet plasma derived from autologous blood, rich in a variety of growth factors. It can promote cell proliferation and differentiation. PRP has been widely used in oral cavity, plastic surgery, orthopedics, neurosurgery and other fields this year. This article reviews the preparation of platelet-rich plasma, the principle of treatment of androgenetic alopecia, and its clinical treatment progress, current problems and future application prospects.

scholarly journals Research Progress in the Mechanism of Effect of PRP in Bone Deficiency Healing

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Ning Zhang ◽  
Yong-Ping Wu ◽  
Sheng-Jun Qian ◽  
Chong Teng ◽  
Shuai Chen ◽  
...  
Keyword(s):  
Bone Repair ◽  
Molecular Mechanisms ◽  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Research Progress ◽  
Current Evidence ◽  
Clinical Settings ◽  
Bone Deficiency ◽  
Defect Repair

Platelet-rich plasma (PRP) therapy is a recently developed technique that uses a concentrated portion of autologous blood to try to improve and accelerate the healing of various tissues. There is a considerable interest in using these PRP products for the treatment used in bone deficiency healing. Because PRP products are safe and easy to prepare and administer, there has been increased attention toward using PRP in numerous clinical settings. The benefits of PRP therapy appear to be promising, and many investigators are exploring the ways in which this therapy can be used in the clinical setting. At present, the molecular mechanisms of bone defect repair studies have focused on three aspects of the inflammatory cytokines, growth factors and angiogenic factors. The role of PRP works mainly through these three aspects of bone repair. The purpose of this paper is to review the current evidence on the mechanism of the effect of PRP in bone deficiency healing.

Changes of alkaline phosphatase activity in response to different stressors in planarian Dugesia japonica

Biologia ◽  
2013 ◽  
Vol 68 (2) ◽  
Author(s):  
Guang-Wen Chen ◽  
Ke-Xue Ma ◽  
De-Zeng Liu
Keyword(s):  
Alkaline Phosphatase ◽  
Heat Shock ◽  
Early Stage ◽  
High Concentration ◽  
Experimental Conditions ◽  
Alp Activity ◽  
Planarian Regeneration ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Dugesia Japonica

AbstractThe aims of this work are to provide some properties of alkaline phosphatase (ALP) in the planarian Dugesia japonica and detect its activity in response to different stressors, as well as to introduce renatured SDS-PAGE to study enzyme activity in planarians. Our results indicate that ALPs in planarians are mainly membrane-bound form, identified as three mainly enzyme-bands (approximately MW 260 kD, 180 kD, 160 kD, respectively). Under our experimental conditions, ALPs activity had no apparent changes in response to low concentration of Hg2+ (25 μg L−1) and Pb2+ (125 μg L−1, 250 μg L−1) exposure, but were severely inhibited in response to high concentration of Hg2+ (50 μg L−1, 150 μg L−1, 300 μg L−1) and Pb2+ (500 μg L−1, 1000 μg L−1) exposure. Mild heat shock (25°C for 2 days) elevated ALP activity, but severely heat shock (25°C for 2 days, followed by 30°C for 2 days and 32°C for 2 days) inactivated ALP activity. Interestingly, ALP and other cytosolic phosphatases (MW from ∼45 kD to ∼60 kD) activity increased noticeably during the early stage of planarians regeneration, which may be involved in cell proliferation and differentiation. Contrary to regeneration, prolonged starvation suppressed ALP activity. The above findings provide valuable information about the role of ALP in planarian regeneration and for its use as an indicator in ecotoxicology.

scholarly journals Leucocyte-Rich Platelet-Rich Plasma Enhances Fibroblast and Extracellular Matrix Activity: Implications in Wound Healing

2020 ◽  
Vol 21 (18) ◽  
pp. 6519
Author(s):  
Jeannie Devereaux ◽  
Narges Dargahi ◽  
Sarah Fraser ◽  
Kulmira Nurgali ◽  
Dimitrios Kiatos ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Proliferation ◽  
Wound Healing ◽  
Blood Cells ◽  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Human Fibroblasts ◽  
White Blood Cells ◽  
High Concentration ◽  
Gene Expressions

Background: Platelet-rich plasma (PRP) is an autologous blood product that contains a high concentration of platelets and leucocytes, which are fundamental fibroblast proliferation agents. Literature has emerged that offers contradictory findings about leucocytes within PRP. Herein, we elucidated the effects of highly concentrated leucocytes and platelets on human fibroblasts. Methods: Leucocyte-rich, PRP (LR-PRP) and leucocyte-poor, platelet-poor plasma (LP-PPP) were compared to identify their effects on human fibroblasts, including cell proliferation, wound healing and extracellular matrix and adhesion molecule gene expressions. Results: The LR-PRP exhibited 1422.00 ± 317.21 × 103 platelets/µL and 16.36 ± 2.08 × 103 white blood cells/µL whilst the LP-PPP demonstrated lower concentrations of 55.33 ± 10.13 × 103 platelets/µL and 0.8 ± 0.02 × 103 white blood cells/µL. LR-PRP enhanced fibroblast cell proliferation and cell migration, and demonstrated either upregulation or down-regulation gene expression profile of the extracellular matrix and adhesion molecules. Conclusion: LR-PRP has a continuous stimulatory anabolic and ergogenic effect on human fibroblast cells.

scholarly journals Membrane potential (Vmem) measurements during mesenchymal stem cell (MSC) proliferation and osteogenic differentiation

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6341 ◽  
Author(s):  
Mit Balvantray Bhavsar ◽  
Gloria Cato ◽  
Alexander Hauschild ◽  
Liudmila Leppik ◽  
Karla Mychellyne Costa Oliveira ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Membrane Potential ◽  
Osteogenic Differentiation ◽  
Cell Function ◽  
Critical Role ◽  
Promote Cell Proliferation ◽  
Cell Behaviors ◽  
Cell Functions ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Background Electrochemical signals play an important role in cell communication and behavior. Electrically charged ions transported across cell membranes maintain an electrochemical imbalance that gives rise to bioelectric signaling, called membrane potential or Vmem. Vmem plays a key role in numerous inter- and intracellular functions that regulate cell behaviors like proliferation, differentiation and migration, all playing a critical role in embryonic development, healing, and regeneration. Methods With the goal of analyzing the changes in Vmem during cell proliferation and differentiation, here we used direct current electrical stimulation (EStim) to promote cell proliferation and differentiation and simultaneously tracked the corresponding changes in Vmem in adipose derived mesenchymal stem cells (AT-MSC). Results We found that EStim caused increased AT-MSC proliferation that corresponded to Vmem depolarization and increased osteogenic differentiation that corresponded to Vmem hyperpolarization. Taken together, this shows that Vmem changes associated with EStim induced cell proliferation and differentiation can be accurately tracked during these important cell functions. Using this tool to monitor Vmem changes associated with these important cell behaviors we hope to learn more about how these electrochemical cues regulate cell function with the ultimate goal of developing new EStim based treatments capable of controlling healing and regeneration.

scholarly journals Quantitatively Controlled Fabrication of Uniaxially Aligned Nanofibrous Scaffold for Cell Adhesion

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Suk Hee Park ◽  
Jung Woo Hong ◽  
Jennifer Hyunjong Shin ◽  
Dong-Yol Yang
Keyword(s):  
Electrospinning Process ◽  
Dermal Fibroblasts ◽  
Fiber Formation ◽  
Actin Stress Fiber ◽  
Promote Cell Proliferation ◽  
Nanofiber Scaffold ◽  
Cell Proliferation And Differentiation ◽  
Fiber Spacing ◽  
Proliferation And Differentiation ◽  
A Cell

In light of tissue engineering, development of a functional and controllable scaffold which can promote cell proliferation and differentiation is crucial. In this study, we introduce a controllable collection method of the electrospinning process for regularly-distributed and uniaxially oriented nanofiber scaffold and evaluate the effects of aligned nanofiber density on adhesion of dermal fibroblasts. The suggested spinning collector features an inclined void gap, which allows easy transfer of uniformly aligned fibers onto other surfaces. By undergoing multiple transfers, the density of the nanofibers can be quantitatively controlled. The resultant polycaprolactone (PCL) nanofibers had well-defined nanotopography in a 400–600 nm range. Human dermal fibroblasts were seeded on aligned nanofiber scaffolds of different densities achieved by varying the number of transfers. Cell morphology and actin stress fiber formation was accessed after seven days. The experimental results indicate that the contact guidance of the cells along the fiber alignment can be more activated with more than one guidance feature on a cell; that is, the high density of fiber is attained in so much that fiber spacing gets below the cell size.

scholarly journals Insulin increases the density of potassium channels in white adipocytes: possible role in adipogenesis

2002 ◽  
Vol 174 (2) ◽  
pp. 299-307 ◽  
Author(s):  
MP Ramirez-Ponce ◽  
JC Mateos ◽  
JA Bellido
Keyword(s):  
Potassium Channels ◽  
Normal Growth ◽  
Potassium Currents ◽  
High Concentration ◽  
Differentiated Cells ◽  
White Adipocytes ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Inhibition Of Differentiation

We studied the potassium currents in white adipocytes obtained by culturing preadipocytes from rat epididymal tissue, both with insulin (WA(i)) and without insulin (WA(o)), in order to test the role of insulin in the development of voltage-gated potassium channels (K(v)) during adipogenesis. Occasionally, very small potassium currents (I(K,V)) were present in preadipocytes; however these currents were measured in all differentiated cells (adipocytes). WA(i) exhibited greater macroscopic potassium currents than WA(o) with no apparent differences in kinetics or voltage dependence. The current density (pA/ micro m(2)) calculated in WA(i) was higher than in WA(o). Currents were blocked by millimolar concentrations of tetrethylamonium (TEA). The effect of insulin on adipogenesis, both with and without TEA, was analysed. Four days without insulin and three days with insulin were necessary to increase the total number of cells in culture by 2.5-fold. Insulin increased the number of differentiated cells by 73.5%. Cell proliferation and differentiation were inhibited by TEA. Proliferation was affected only by high concentration of TEA. Inhibition of differentiation was dose dependent, with the concentration necessary for half-block similar to the IC(50) values to block potassium channels. These results suggest that insulin increases the density of K(v) and that these channels may be necessary for the normal growth of white adipocytes in culture.

scholarly journals Ultrasonography-guided Platelet-rich Plasma Injection in Chronic Plantar Fasciitis

2017 ◽  
Vol 4 (1) ◽  
pp. 1-4
Author(s):  
Tribhuwan NS Gaur ◽  
Harish Rao ◽  
Garjesh S Rai
Keyword(s):  
Plantar Fasciitis ◽  
Platelet Rich Plasma ◽  
Therapeutic Option ◽  
Autologous Blood ◽  
Heel Pain ◽  
Asia Pacific ◽  
Research Centre ◽  
High Concentration ◽  
Plasma Injection ◽  
Tissue Healing

ABSTRACT Introduction Heel pain is a very common problem. The most common cause of heel pain is plantar fasciitis, and this often refractory to conservative treatment leads to disability. Platelet-rich plasma (PRP) derived from autologous blood containing high concentration of growth factors helps in tissue healing, so it is postulated to promote native tissue regeneration. The purpose of this work was to study the effectiveness of PRP in the treatment of chronic plantar fasciitis. Materials and methods The study was conducted in Orthopedic Department in collaboration with Radiodiagnosis Department at People's College of Medical Sciences and Research Centre, Bhopal, Madhya Pradesh, India. In this study, there were 44 patients, with 48 feet affected by plantar fasciitis. Results All patients were evaluated preinjection and postinjection by visual analog score. There was significant improvement after injection and no adverse reaction was seen. Conclusion Platelet-rich plasma is considered as a safe therapeutic option. It also effectively decreases heel pain in chronic plantar fasciitis. How to cite this article Gaur TNS, Rao H, Rai GS. Ultrasonography-guided Platelet-rich Plasma Injection in Chronic Plantar Fasciitis. J Foot Ankle Surg (Asia-Pacific) 2017;4(1):1-4.

Platelet-rich plasma enhances human osteoblast-like cell proliferation and differentiation

2005 ◽  
Vol 63 (3) ◽  
pp. 362-369 ◽  
Author(s):  
Takahiro Kanno ◽  
Tetsu Takahashi ◽  
Toshiyuki Tsujisawa ◽  
Wataru Ariyoshi ◽  
Tatsuji Nishihara
Keyword(s):  
Cell Proliferation ◽  
Platelet Rich Plasma ◽  
Human Osteoblast ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

scholarly journals Platelet Rich Plasma for Treatment of Rheumatoid Arthritis: Case Series and Review of Literature

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Humeira Badsha ◽  
Ghita Harifi ◽  
William D. Murrell
Keyword(s):  
Rheumatoid Arthritis ◽  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Treatment Options ◽  
Case Series ◽  
Synovial Cell ◽  
Joint Inflammation ◽  
Inadequate Response ◽  
Muscle Injuries ◽  
Cell Proliferation And Differentiation

Platelet-rich plasma (PRP) is an autologous blood product with platelets above circulating levels and releases several growth factors after activation. PRP may help to decrease joint inflammation by modulating synovial cell proliferation and differentiation and inhibition of catabolic pathways in various articular conditions. Though PRP has shown good efficacy in osteoarthritis and other musculoskeletal conditions such as synovitis, epicondylitis, skeletal muscle injuries, and tendinopathy, there is limited experience for the use of PRP in patients with rheumatoid arthritis. Precise mechanisms of action of PRP are not known. We present clinical experience for treatment with PRP (2–4 ml) in four patients with rheumatoid arthritis who had inadequate response and persistent pain and inflammation with intra-articular steroids. Irrespective of past and ongoing treatments and duration of disease, all patients showed improvement in the visual analog scale and disease activity score of 28 joints at 4 and 8 weeks after injection. There was an improvement in joint inflammation on ultrasound imaging in some patients. These effects were sustained for up to 1 year. No adverse effects were reported in any patient. PRP may be a safe and useful therapy in patients with rheumatoid arthritis who fail to respond to one or more established treatment options.

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