Comparison of secretory acid proteinase, phospholipase activity, and slime production in Candida parapsilosis complex strains isolated from blood cultures and other clinical samples

2017 ◽  
Author(s):  
Nejla Cebeci Güler ◽  
İlknur Tosun
Keyword(s):  
Candida Parapsilosis ◽  
Blood Cultures ◽  
Clinical Samples ◽  
Acid Proteinase ◽  
Phospholipase Activity ◽  
Slime Production ◽  
Candida Parapsilosis Complex

scholarly journals Molecular identification and antifungal susceptibility profiles of Candida parapsilosis complex species isolated from culture collection of clinical samples

2015 ◽  
Vol 48 (4) ◽  
pp. 454-459 ◽  
Author(s):  
Fábio Silvestre Ataides ◽  
Carolina Rodrigues Costa ◽  
Lúcia Kioko Hasimoto e Souza ◽  
Orionalda deFátima Lisboa Fernandes ◽  
Rosália Santos Amorim Jesuino ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Culture Collection ◽  
Clinical Samples ◽  
Complex Species ◽  
Candida Parapsilosis Complex ◽  
Susceptibility Profiles

Exposure of Candida parapsilosis complex to agricultural azoles: An overview of the role of environmental determinants for the development of resistance

2019 ◽  
Vol 650 ◽  
pp. 1231-1238 ◽  
Author(s):  
Raimunda Sâmia Nogueira Brilhante ◽  
Lucas Pereira de Alencar ◽  
Silviane Praciano Bandeira ◽  
Jamille Alencar Sales ◽  
Antônio José de Jesus Evangelista ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Environmental Determinants ◽  
Development Of Resistance ◽  
Candida Parapsilosis Complex

Genotypic variation and slime production among blood and catheter isolates of Candida parapsilosis.

1994 ◽  
Vol 32 (2) ◽  
pp. 452-456 ◽  
Author(s):  
M L Branchini ◽  
M A Pfaller ◽  
J Rhine-Chalberg ◽  
T Frempong ◽  
H D Isenberg
Keyword(s):  
Candida Parapsilosis ◽  
Genotypic Variation ◽  
Slime Production

scholarly journals Rapid Detection of Echinocandins Resistance by MALDI-TOF MS in Candida parapsilosis Complex

2020 ◽  
Vol 8 (1) ◽  
pp. 109
Author(s):  
Ana Emília M. Roberto ◽  
Danilo E. Xavier ◽  
Esteban E. Vidal ◽  
Cláudia Fernanda de L. Vidal ◽  
Rejane P. Neves ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Incubation Time ◽  
Candida Parapsilosis ◽  
Clinical Laboratory ◽  
Antifungal Susceptibility ◽  
Reference Method ◽  
Sensu Stricto ◽  
Maldi Tof ◽  
Separation Parameter ◽  
Candida Parapsilosis Complex

Mass spectrometry by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) was used to identify and differentiate the pattern of susceptibility of clinical isolates of Candida parapsilosis complex. 17 C. parapsilosis sensu stricto, 2 C. orthopsilosis, and 1 C. metapsilosis strains were obtained from blood cultures, and three different inocula (103, 105, and 107 CFU/mL) were evaluated against three echinocandins at concentrations ranging from 0.03 to 16 µg/mL after incubation of 1 h, 2 h, and 3 h. Drug-free control was used. The spectra obtained at these concentrations were applied to generate composite correlation index (CCI) matrices for each yeast individually. After cross correlations and autocorrelations of each spectra with null (zero) and maximal (16) concentrations, the CCI was used as separation parameter among spectra. Incubation time and inoculum were critical factors to reach higher precision and reliability of this trial. With an incubation time of 3 h and inoculum of 107 CFU/mL, it was possible to determine the breakpoint of the clinical yeasts by MALDI-TOF that presented high agreement with the clinical laboratory standard institute (CLSI) reference method. Herein, we show that mass spectrometry using the MALDI-TOF technique is powerful when it exploits antifungal susceptibility testing assays.

scholarly journals Capability of Fluorescent Capillary Electrophoresis To Distinguish Species of the Candida parapsilosis Complex

2019 ◽  
Vol 57 (5) ◽  
Author(s):  
Iva Kotásková ◽  
Hana Obručova ◽  
Veronika Lýčková ◽  
Filip Růžička ◽  
Tomáš Freiberger
Keyword(s):  
Capillary Electrophoresis ◽  
Candida Parapsilosis ◽  
Candida Parapsilosis Complex

scholarly journals Virulence Factors Contributing to Pathogenicity ofCandida tropicalisand Its Antifungal Susceptibility Profile

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Sachin C. Deorukhkar ◽  
Santosh Saini ◽  
Stephen Mathew
Keyword(s):  
Virulence Factors ◽  
Biofilm Formation ◽  
Antifungal Susceptibility ◽  
Common Species ◽  
Blood Cultures ◽  
Haemolytic Activity ◽  
Phospholipase Activity ◽  
Fluconazole Resistance ◽  
Activity Maximum ◽  
The Past

The incidence of invasive candidiasis has increased over the past few decades. AlthoughCandida albicansremains by far the most common species encountered, in recent years shift towards non-albicans Candidaspecies likeCandida tropicalisis noted. Here in this study we determined the virulence factors and antifungal susceptibility profile of 125C. tropicalisisolated from various clinical specimens. Biofilm formation was seen in 53 (42.4%) isolates. Coagulase production was noted in 18 (14.4%) isolates. Phospholipase enzyme was the major virulent factor produced byC. tropicalisisolates. A total of 39 biofilm forming isolates showed phospholipase activity. Proteinase activity was demonstrated by 65 (52%) isolates. A total of 38 (30.4%) isolates showed haemolytic activity. Maximum isolates demonstrated resistance to fluconazole. Fluconazole resistance was more common inC. tropicalisisolated from blood cultures. Antifungal resistance was more in isolates possessing the ability to produce phospholipase and biofilm.C. tropicalisexhibit a great degree of variation not only in their pathogenicity but also in their antifungal susceptibility profile. The identification of virulence attributes specific for each species and their correlation with each other will aid in the understanding of the pathogenesis of infection.

scholarly journals Identification and typing of the Candida parapsilosis complex: MALDI-TOF MS vs. AFLP

2014 ◽  
Vol 52 (2) ◽  
pp. 123-130 ◽  
Author(s):  
E. D. Carolis ◽  
L. A. M. Hensgens ◽  
A. Vella ◽  
B. Posteraro ◽  
M. Sanguinetti ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Candida Parapsilosis Complex ◽  
Tof Ms
Sign in / Sign up

Export Citation Format

Share Document