scholarly journals Antibiotic Resistance Mechanisms of Acinetobacter baumannii

ANKEM Dergisi ◽  
2011 ◽  
Vol 25 (3) ◽  
pp. 196-207
Author(s):  
Ihsan Hakki Ciftci ◽  
Gulsah Asik
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Resistance Mechanisms

Antibiotic Resistance Mechanisms and Their Transmission in Acinetobacter baumannii

2021 ◽  
pp. 135-153
Author(s):  
Ronan R. McCarthy ◽  
Gerald J. Larrouy-Maumus ◽  
Mei Gei C. Meiqi Tan ◽  
David W. Wareham
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Resistance Mechanisms

scholarly journals In vivo protein interaction network analysis reveals porin-localized antibiotic inactivation in Acinetobacter baumannii strain AB5075

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Xia Wu ◽  
Juan D. Chavez ◽  
Devin K. Schweppe ◽  
Chunxiang Zheng ◽  
Chad R. Weisbrod ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Outer Membrane ◽  
Protein Interaction ◽  
Protein Interaction Network ◽  
Outer Membrane Proteins ◽  
Interaction Network ◽  
Resistance Mechanisms ◽  
Clinical Strain

Abstract The nosocomial pathogen Acinetobacter baumannii is a frequent cause of hospital-acquired infections worldwide and is a challenge for treatment due to its evolved resistance to antibiotics, including carbapenems. Here, to gain insight on A. baumannii antibiotic resistance mechanisms, we analyse the protein interaction network of a multidrug-resistant A. baumannii clinical strain (AB5075). Using in vivo chemical cross-linking and mass spectrometry, we identify 2,068 non-redundant cross-linked peptide pairs containing 245 intra- and 398 inter-molecular interactions. Outer membrane proteins OmpA and YiaD, and carbapenemase Oxa-23 are hubs of the identified interaction network. Eighteen novel interactors of Oxa-23 are identified. Interactions of Oxa-23 with outer membrane porins OmpA and CarO are verified with co-immunoprecipitation analysis. Furthermore, transposon mutagenesis of oxa-23 or interactors of Oxa-23 demonstrates changes in meropenem or imipenem sensitivity in strain AB5075. These results provide a view of porin-localized antibiotic inactivation and increase understanding of bacterial antibiotic resistance mechanisms.

scholarly journals Antibiotic Resistance Profiles, Molecular Mechanisms and Innovative Treatment Strategies of Acinetobacter baumannii

2020 ◽  
Vol 8 (6) ◽  
pp. 935 ◽  
Author(s):  
Corneliu Ovidiu Vrancianu ◽  
Irina Gheorghe ◽  
Ilda Barbu Czobor ◽  
Mariana Carmen Chifiriuc
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Molecular Mechanisms ◽  
Treatment Strategies ◽  
Multidrug Resistant ◽  
Resistance Mechanisms ◽  
Negative Bacterium ◽  
Innovative Strategies ◽  
Industry Environment ◽  
Underlying Mechanisms

Antibiotic resistance is one of the biggest challenges for the clinical sector and industry, environment and societal development. One of the most important pathogens responsible for severe nosocomial infections is Acinetobacter baumannii, a Gram-negative bacterium from the Moraxellaceae family, due to its various resistance mechanisms, such as the β-lactamases production, efflux pumps, decreased membrane permeability and altered target site of the antibiotic. The enormous adaptive capacity of A. baumannii and the acquisition and transfer of antibiotic resistance determinants contribute to the ineffectiveness of most current therapeutic strategies, including last-line or combined antibiotic therapy. In this review, we will present an update of the antibiotic resistance profiles and underlying mechanisms in A. baumannii and the current progress in developing innovative strategies for combating multidrug-resistant A. baumannii (MDRAB) infections.

scholarly journals Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii From Khartoum State, Sudan

2021 ◽  
Vol 12 ◽  
Author(s):  
Leena Al-Hassan ◽  
Hana Elbadawi ◽  
Einas Osman ◽  
Sara Ali ◽  
Kamal Elhag ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Molecular Epidemiology ◽  
Acinetobacter Baumannii ◽  
Core Genome ◽  
Treatment Options ◽  
Resistance Mechanisms ◽  
Multiple Antibiotic Resistance ◽  
Resistance Determinants ◽  
Carbapenem Resistant ◽  
Allelic Differences

Carbapenem resistant Acinetobacter baumannii (CRAb) is an important global pathogen contributing to increased morbidity and mortality in hospitalized patients, due to limited alternative treatment options. Nine international clonal (IC) lineages have been identified in many countries worldwide, however, data still lacks from some parts of the world, particularly in Africa. We hereby present the molecular epidemiology of MDR A. baumannii from four hospitals in Khartoum, Sudan, collected from 2017 to 2018. Forty-two isolates were whole-genome sequenced, and subsequent molecular epidemiology was determined by core genome MLST (cgMLST), and their resistomes identified. All isolates had an array of diverse antibiotic resistance mechanisms conferring resistance to multiple classes of antibiotics. We found a predominance (88%) of IC2 (with the intrinsic OXA-66 and acquired OXA-23), and some with NDM-1. IC2 isolates were sub-divided into 4 STs separated by 5 to 431 allelic differences, and with evidence of seven transmission clusters. Isolates belonging to IC1, IC5, and IC9 were also identified. These data illustrate that MDR IC2 A. baumannii are widely distributed in Khartoum hospitals and are in possession of multiple antibiotic resistance determinants.

scholarly journals Microbiological and Molecular Genetic Aspects of Antibiotic Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii

2020 ◽  
Vol 18 (6) ◽  
pp. 34-38
Author(s):  
Yu. E. Skurikhina ◽  
V. B. Turkutyukov
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Molecular Genetic ◽  
Resistance Mechanisms ◽  
Steady Increase ◽  
Molecular Genetic Study ◽  
Antibiotic Resistant ◽  
Resistant Strains ◽  
High Level

Relevance. The increase in the frequency of infections caused by Pseudomonas aeruginosa and Acinetobacter baumannii, which have a high level of resistance to many groups of antibiotics, requires a comprehensive study, including modern research methods.Aims. The study of regional features of the dynamics of the formation and circulation of antibiotic-resistant strains A. baumannii and P. aeruginosa.Materials and methods. During 2009-2018 we analyzed the data of microbiological laboratories of multidisciplinary hospitals and carried out a molecular genetic study of the determinants of antibiotic resistance by PCR of A. baumannii and P. aeruginosa strains isolated from clinical material in order to determine the level of variability of resistance.Results. The study revealed a tendency to increase in the proportion of strains A. baumannii and P. aeruginosa in the etiological structure of healh-care associated infections and purulent-septic infections; high incidence of strains resistant to cephalosporins, carbapenems, beta-lactams and multi-resistant strains. The appearance and distribution of the determinants of antibiotic resistance NDM-1 and MCR-1 in these bacteria were also detected.Conclusions. Over the past decade, a steady increase in the proportion of A. baumannii and P. Aeruginosa resistant to many antibiotics in patients in intensive care unit and surgery departments in hospitals of Vladivostok (Primorsky reg., Russia), and the emergence of new antimicrobial resistance mechanisms in these microorganisms.

scholarly journals Escaping from ESKAPE. Clinical Significance and Antibiotic Resistance Mechanisms in Acinetobacter baumannii: a Review

2020 ◽  
Vol 11 (1) ◽  
pp. 8190-8203
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Nosocomial Infections ◽  
Antimicrobial Agents ◽  
Genetic Resistance ◽  
Resistance Mechanisms ◽  
Genomic Islands ◽  
Resistant Bacteria ◽  
Clinical Settings

Antibiotic resistance represents a critical threat in clinical settings nowadays, with an essential ecological dimension. Due to the involvement of the resistance genes, this phenomenon has gained an unprecedented expansion. Their accumulation and dissemination are facilitated by mobile genetic elements (MGEs) (plasmids, transposons, integrons, genomic islands) that can increase intracellular DNA mobility. In clinical settings, one of the critical resistant bacteria associated with nosocomial infections is Acinetobacter baumannii. This Gram-negative bacterium exhibits variate resistance mechanisms that enable it to survive in extreme environmental conditions and to evade antimicrobial agents. The enormous adaptive capacity and the essential role in the emergence of severe nosocomial infections lead to the need to study more deeply the mechanisms involved in antibiotic resistance in A. baumannii strains. In this review, we will initially present the role of A. baumannii in human and veterinary infectious pathology. We will subsequently discuss the main genetic resistance mechanisms (both intrinsic and acquired) encountered in A. baumannii strains.

MICROORGANISMS ANTIBIOTIC RESISTANCE MECHANISMS – PRESENT VIEW

2019 ◽  
Vol 21 (10) ◽  
pp. 125-130
Author(s):  
Пушилина А.Д. ◽  
◽  
Коменкова Т.С. ◽  
Зайцева Е.А. ◽  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Mechanisms ◽  
Present View

scholarly journals Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determines the Outcome?

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 833
Author(s):  
Camila Pimentel ◽  
Casin Le ◽  
Marisel R. Tuttobene ◽  
Tomas Subils ◽  
Krisztina M. Papp-Wallace ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Human Serum Albumin ◽  
Quorum Sensing ◽  
Serum Albumin ◽  
Human Serum ◽  
Acinetobacter Baumannii ◽  
Biofilm Formation ◽  
Model Systems ◽  
Expression Levels ◽  
Multiple Drugs

Acinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of pbp1 and pbp3 tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A. baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains.

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