scholarly journals The effect of Sorghum Tempeh (Sorghum bicolor L. Moench) on low-density lipoprotein (LDL) and malondialdehyde (MDA) levels in atherogenic diet-induced rats

10.5219/1589 ◽  
2021 ◽  
Vol 15 ◽  
pp. 662-671
Author(s):  
Ana Khoirun Nisa ◽  
Diana Nur Afifah ◽  
Kis Djamiatun ◽  
Ahmad Syauqy
Keyword(s):  
Total Cholesterol ◽  
Normal Control ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Atherogenic Diet ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Sorghum Flour ◽  
Before And After

An atherogenic diet induces oxidative stress leading to hypercholesterolemia. This condition causes atherosclerosis followed by increased LDL and MDA. Sorghum tempeh contains fiber and antioxidants that can protectively improve LDL and MDA levels. Therefore, this research aims to determine the effect of sorghum tempeh on LDL and MDA levels in atherogenic diet-induced rats compared to sorghum flour. It used a randomized pre-post test with a control group design. The test subjects were 30 male Sprague Dawley rats, consisting of 6 normal conditioned rats (C1), and 24 that were induced by an atherogenic diet (C2, T1, T2, T3) for 2 weeks. Sorghum flour was administered at a dose of 4.095 g (T1) and the sorghum tempeh at 3.041 g (T2) and 6.081 g (T3) for 4 weeks. Furthermore, C2 was constantly induced through an atherogenic diet. Total cholesterol and LDL levels were then analyzed using the CHOD-PAP method, and MDA levels, using the ELISA method. Meanwhile, statistical analysis for these variables was carried out using IBM SPSS Statistics 21 software. The results showed that the administration of sorghum flour and tempeh significantly reduced total cholesterol, LDL, MDA levels in each group (p = 0.001). Furthermore, it showed that there was a significantly strong correlation between LDL and MDA levels before and after treatment (r = 0.610, r = 0.805, and p = 0.001). The administration of sorghum tempeh at a dose of 6.081 g caused the greatest reduction (∆) in LDL levels at -44.19 ±2.58 mg.dL-1, although, it was not the same as normal control. Meanwhile, sorghum flour at a dose of 4.095 g was the most influential in reducing MDA levels to the same as normal control with delta (∆) at -7.67 ±0.37 ng.mL-1. In conclusion, sorghum tempeh and flour were the most effective at reducing LDL and MDA levels, respectively.

scholarly journals The Correlation of Use of Depo Medroxy Progesterone Acetate (DMPA) In-jections with Lipid Profile Levels in Rats

2021 ◽  
Vol 4 (1) ◽  
pp. 27-31
Author(s):  
Suratiah Suratiah ◽  
Dewa Ayu Surinati ◽  
I Dewa Gede Putu Putra Yasa
Keyword(s):  
Lipid Profile ◽  
Total Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Control Group ◽  
Control Group Design ◽  
Cholesterol Levels ◽  
Significant Difference ◽  
Before And After ◽  
Long Time

Introduction: Family Planning is a national strategy of Indonesia government to manage the population growth. Depo Medroxyprogesterone Acetate (DMPA) is one of injectable contraceptives most widely used because it is simple and easy to obtain. However, it has various side effects causing imbalance of hormone estrogen, in turns to result in a decrease in HDL (High Density Lipoprotein) and an increase in LDL (Low Density Lipoprotein) which will result in an increase in total cholesterol. It will also affect changes in fat metabolism in human body due to hormonal influences. This results in dyslipidemia and atherosclerosis. Method: The method in this study is an experimental study with a pretest-posttest control group design. Results: The purpose of this study was to determine the relationship between duration of use of DMPA injections with lipid profile levels in mice. The data were analyzed by using the Paired t-test parametric test to compare between treatment groups. This study found that there were significant differences in HDL levels and total cholesterol levels between before and after administration of DMPA injections on the 14th and 35th days. There is a significant relationship between the duration of administration of DMPA injections with HDL levels and total cholesterol levels in mice. However, there was no difference in LDL levels and triglyceride levels between before and after administration of DMPA injection on day of 14 and day of 35, while, there was a significant difference between before and after the 35th day. There is no relationship between duration of administration of DMPA injections with LDL levels, while there is a relationship among mice triglycerides. Conclusions: Administration of DMPA injections for a long time lowers HDL.

scholarly journals PENGARUH PEMBERIAN EKSTRAK BUNGA PEPAYA (Carica papaya L) TERHADAP KADAR HIGH DENSITY LIPOPROTEIN (HDL) DAN LOW DENSITY LIPOPROTEIN (LDL) DARAH TIKUS (Rattus norvegicus) GALUR Sprague dawley JANTAN YANG DIBERI DIET TINGGI LEMAK

2019 ◽  
Vol 6 (4) ◽  
pp. 247-256
Author(s):  
Dita Fitriani ◽  
Hetti Rusmini ◽  
Yuliana Wildani Marek
Keyword(s):  
Rattus Norvegicus ◽  
Carica Papaya ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Control Group ◽  
Low Density ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Hiperlipidemia merupakan suatu keadaan patologis akibat kelainan metabolisme lipid yang ditandai dengan peningkatan fraksi lipid di dalam darah. Bunga pepaya mengandung senyawa yang mampu mempertahankan kadar kolesterol dalam batas normal karena mengandung senyawa flavanoid dan tanin yang berperan sebagai antioksidan. Pada penelitian ini digunakan tikus sebagai hewan coba untuk mengetahui pengaruh pemberian ekstrak bunga pepaya (Carica papaya L) terhadap kadar HDL dan LDL darah tikus yang diberi diet tinggi lemak. Penelitian ini menggunakan 25 ekor tikus Sprague dawley jantan berusia 12-16 minggu dengan berat 200-250 g, dengan pre and post test with control group design. Tikus dibagi menjadi 5 kelompok yaitu; kelompok tikus yang hanya diberi pakan standar (K), kelompok tikus yang diberi diet tinggi lemak (KN), kelompok tikus yang diberi diet tinggi lemak dan ekstrak bunga pepaya 31 mg/kgBB (P1), kelompok tikus yang diberi diet tinggi lemak dan ekstrak bunga pepaya 62 mg/kgBB (P2), dan kelompok tikus yang diberi diet tinggi lemak dan ekstrak bunga pepaya 125 mg/kgBB (P3). Hasil penelitian menunjukkan bahwa pemberian ekstrak bunga pepaya dengan dosis 31 mg/kgBB, 62 mg/kgBB dan 125 mg/kgBB mampu meningkatkan rerata kadar HDL dan menurunkan rerata kadar LDL darah tikus secara signifikan (p <0,05). Namun semakin besar dosis tidak berpengaruh terhadap kadar HDL dan LDL.

scholarly journals PERBEDAAN PERUBAHAN KADAR KOLESTEROL LDL SETELAH PEMBERIAN EKSTRAK DAN REBUSAN DAUN SALAM (EUGENIA POLYANTHA) PADA TIKUS SPRAGUE DAWLEY YANG DIBERI PAKAN TINGGI LEMAK

2014 ◽  
Vol 3 (1) ◽  
pp. 90-97
Author(s):  
Astri Praba Shinta ◽  
Martha Irene Kartasurya
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Randomized Control ◽  
Post Hoc ◽  
Paired T Test

LatarBelakang : Dislipidemia merupakan gangguan metabolisme lipid berupa peningkatan kadar kolesterol total, kolesterol Low Density Lipoprotein (LDL), trigliserida, dan penurunan kolesterol High Density Lipoprotein (HDL). Quercetin yang terkandung dalam flavonoid daun salam dapat menurunkan kadar kolesterol LDL.Metode : Jenis penelitian ini adalah eksperimental laboratorik dengan pre-post test randomized control group design. Subjek penelitian adalah 24 tikus Sprague Dawley jantan berumur 8 minggu yang dibagi menjadi 4 kelompok, yang terdiri dari 6 ekor tikus. Keempat kelompok diberi pakan standar dan diet tinggi lemak. Kelompok pertama merupakan kelompok kontrol positif dan kelompok kedua diberi 0,18 g/kg BB simvastatin. Kelompok ketiga diberi 0,034 g/kg BB ekstrak daun salam dan kelompok keempat diberi 0,72 g/kg BB rebusan daun salam. Kadar kolesterol LDL ditentukan dengan metode CHOD-PAP. Normalitas distribusi data diuji dengan Shapiro-Wilks. Data dianalisis dengan uji paired t-test, Kruskall Wallis, dan One Way Anova.Hasil : Hasil penelitian menunjukkan adanya peningkatan kadar kolesterol LDL yaitu 79,7 mg/dL pada kelompok kontrol positif; 38,3 mg/dL pada kelompok rebusan; 26,2 mg/dL pada kelompok ekstrak; serta 2,9 mg/dL pada kelompok simvastatin. Kelompok kontrol positif mengalami peningkatan kadar kolesterol LDL paling tinggi dibandingkan dengan kelompok lainnya. Peningkatan kadar kolesterol LDL pada kelompok ekstrak (26,2 mg/dL) lebih rendah dibandingkan dengan kelompok rebusan (38,3 mg/dL). Hasil uji Post Hoc menunjukkan bahwa terdapat perbedaan perubahan kadar kolesterol LDL antara kelompok simvastatin dan kontrol positif; simvastatin dan ekstrak; simvastatin dan rebusan; ekstrak dan kontrol positif; ekstrak dan rebusan; serta rebusan dan kontrol positif. Simpulan : Pemberian rebusan daun salam tidak dapat menurunkan kadar kolesterol LDL pada tikus Sprague Dawley yang diberi pakan tinggi lemak namun peningkatan kadar kolesterol LDL pada kelompok rebusan lebih rendah dibandingkan dengan kelompok kontrol positif serta lebih tinggi peningkatannya dibandingkan dengan kelompok ekstrak dan simvastatin.

scholarly journals PENGARUH PEMBERIAN JUS BIJI PEPAYA (CARICA PAPAYA L.) TERHADAP RASIO KOLESTEROL LDL:HDL TIKUS SPRAGUE DAWLEY DISLIPIDEMIA

2013 ◽  
Vol 2 (3) ◽  
pp. 302-311
Author(s):  
Dyah Agustina ◽  
Hesti Murwani R
Keyword(s):  
Carica Papaya ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Randomized Control ◽  
One Way Anova

Latar Belakang   : Dislipidemia sebagai faktor risiko utama penyakit kardiovaskular merupakan suatu keadaan yang ditandai dengan peningkatan konsentrasi kolesterol total, kolesterol LDL (Low Density Lipoprotein), dan trigliserida serta penurunan konsentrasi kolesterol HDL (High Density Lipoprotein) dari batas normal. Parameter paling akurat dalam memprediksi penyakit tersebut adalah perbandingan nilai kolesterol LDL dan HDL yang disebut rasio kolesterol LDL:HDL. Pengendalian dislipidemia dapat dilakukan melalui modifikasi diet, salah satunya dengan meningkatkan konsumsi makanan sumber efek hipolipidemik. Biji pepaya merupakan biji yang memiliki efek hipolipidemia dan antioksidan melalui zat fitokimia yang dikandungnya meliputi flavonoid, saponin dan tannin. Tujuan : Menganalisis pengaruh pemberian jus biji pepaya terhadap rasio kolesterol LDL:HDL tikus Sprague Dawley dislipidemia.Hasil : Pemberian jus biji pepaya yang mengandung 646,1 mg flavonoid, 69,3 mg saponin, dan 140,9 mg tanin tiap 100 gram pada dosis 400 mg dan 800 mg mampu menurunkan rasio kolesterol LDL:HDL dari 2,64±0,70 menjadi 2,25±0,54 dan 2,94±1,01 menjadi 2,11±0,65 pada tikus Sprague Dawley dislipidemia dengan diet kolesterol 42,7834 mg/hari, namun tidak bermakna (p>0,05). Tidak terdapat perbedaan bermakna (p>0,05) antar kelompok.Metode : Jenis penelitian ini adalah true-experimental laboratorik dengan pre and post test with randomized control group design. Subjek penelitian yaitu 28 ekor tikus Sprague Dawley jantan berusia 8 minggu dengan berat 150-180 gram yang diinduksi dislipidemia, diberi jus biji pepaya dengan dosis 400 mg dan 800 mg per hari selama 30 hari. Rasio kolesterol LDL:HDL didapat dari perbandingan kadar kolesterol LDL dengan kolesterol HDL dimana kolesterol LDL dan kolesterol HDL ditentukan menggunakan reagen diasis dan metode CHOD-PAP. Normalitas data diuji dengan Shapiro Wilks. Data dianalisis menggunakan uji Paired t-test, One Way Anova dan LSD.Simpulan : Jus biji pepaya pada dosis 400 mg/ekor/hari dan 800 mg/ekor/hari selama 30 hari mampu menurunkan rasio kolesterol LDL:HDL tikus Sprague Dawley dislipidemia, namun tidak bermakna. Tidak terdapat perbedaan efektivitas antara dosis 400 mg dan 800 mg.

Impaired postprandial clearance of triacylglycerol-rich lipoproteins in adipose tissue in obese subjects

1995 ◽  
Vol 268 (4) ◽  
pp. E588-E594 ◽  
Author(s):  
J. L. Potts ◽  
S. W. Coppack ◽  
R. M. Fisher ◽  
S. M. Humphreys ◽  
G. F. Gibbons ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Low Density Lipoprotein ◽  
Postprandial Lipemia ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Control Group ◽  
Postprandial Period ◽  
Before And After ◽  
Obese Subjects ◽  
Subcutaneous Adipose

Adipose tissue is an important site of clearance of circulating triacylglycerol (TAG), especially in the postprandial period. Postprandial lipemia is usually increased in obesity. We studied the extraction of TAG from plasma and TAG-rich lipoproteins (TRLs) in subcutaneous adipose tissue in 11 control and 8 obese subjects before and after a mixed meal. Clearance of plasma TAG and very low-density lipoprotein (VLDL)-TAG was decreased in the obese subjects after an overnight fast. After the meal, chylomicron-TAG extraction increased in the control group whereas VLDL-TAG clearance decreased; these changes were not seen in the obese group, in whom the VLDL particles appeared to be better able to compete with the chylomicrons for clearance by lipoprotein lipase. In the control subjects, removal of TAG from the TRL in the postprandial period was accompanied by a shift toward addition of cholesterol to the high-density lipoprotein (HDL) fraction; this was not observed in the obese subjects. We conclude that disturbed TRL-TAG clearance in adipose tissue is related both to the elevated plasma TAG concentrations and the depressed HDL-cholesterol concentrations typical of obesity.

scholarly journals Efficacy of Myrmecodia Platytyrae (MyP) Water Extract in Reducing Cholesterol Level in Hypercholesterolemia Induced Sprague Dawley Rat

2020 ◽  
Vol 7 (1) ◽  
pp. 27-38
Author(s):  
M. K. Nik Hasan ◽  
I. Abdul Wahab ◽  
H. H. Mizaton ◽  
M. A. Rasadah
Keyword(s):  
Low Density Lipoprotein ◽  
Water Extract ◽  
Density Lipoprotein ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Medicinal Value ◽  
Ant Nest ◽  
Trees And Shrubs

Myrmecodia plant or ant-nest plant is from Rubiaceae family. Rubiaceae are mainly tropical woody plants, consist mostly of trees and shrubs and can be found in temperate regions. Myrmecodia platytyrea (MyP) are believed to have medicinal value.  This study was designed in order to investigate the effect of MyP extract as anti hypercholesterolemic agent. The results showed that treatment of MyP can significantly reduce (p<0.05) low density lipoprotein (LDL) compared to negative control group. The extract was significantly increase (p<0.05) high density lipoprotein (HDL) concentration compared to negative control group. Besides that, MyP increased fecal cholesterol and fecal bile compared to normal control group. It was also found that lipid profile was significantly decreased (p<0.05) in MyP treatment group. All biochemistry data showed that MyP water extract was not toxic at all.

scholarly journals Efficacy of Myrmecodia Platytyrae (MyP) Water Extract in Reducing Cholesterol Level in Hypercholesterolemia Induced Sprague Dawley Rat

2020 ◽  
Vol 7 (2) ◽  
pp. 69-80
Author(s):  
M. K. Nik Hasan ◽  
I. Abdul Wahab ◽  
H. H. Mizaton ◽  
M. A. Rasadah
Keyword(s):  
Low Density Lipoprotein ◽  
Water Extract ◽  
Density Lipoprotein ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Medicinal Value ◽  
Ant Nest ◽  
Trees And Shrubs

Myrmecodia plant or ant-nest plant is from Rubiaceae family. Rubiaceae are mainly tropical woody plants, consist mostly of trees and shrubs and can be found in temperate regions. Myrmecodia platytyrea (MyP) are believed to have medicinal value.  This study was designed in order to investigate the effect of MyP extract as anti hypercholesterolemic agent. The results showed that treatment of MyP can significantly reduce (p<0.05) low density lipoprotein (LDL) compared to negative control group. The extract was significantly increase (p<0.05) high density lipoprotein (HDL) concentration compared to negative control group. Besides that, MyP increased fecal cholesterol and fecal bile compared to normal control group. It was also found that lipid profile was significantly decreased (p<0.05) in MyP treatment group. All biochemistry data showed that MyP water extract was not toxic at all.

scholarly journals RED ROSELLA TEA AND AVOCADO AS SIMVASTATIN THERAPY SUPPORT REDUCE TOTAL CHOLESTEROL

Jurnal NERS ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 113
Author(s):  
Budi Artini ◽  
Elyana Asnar ◽  
Ika Yuni Widyawati
Keyword(s):  
Total Cholesterol ◽  
Health Workers ◽  
Total Cholesterol Level ◽  
Control Group ◽  
Control Group Design ◽  
Simvastatin Therapy ◽  
Cholesterol Levels ◽  
Significant Difference ◽  
Before And After ◽  
Quasi Experimental

Introduction: Hypercholesterolemia is a condition characterized by high levels of total cholesterol in the blood. Many studies have proven that steeping tea rosella and flesh of an avocado can reduce total cholesterol levels. This study was conducted to determine the effectiveness of therapy companion rosella tea and  avocado in lowering total cholesterol levels in hypercholesterolemia clients. Method: This type of research is a quasi-experimental study with pre-post test control group design. The population study was a client with hypercholesterolemia in the working area of menganti health centers. First sample group consisted of nine respondents received the drug Simvastatin 10 mg and rosella tea consumed as much as 2 g 1x / day. The second group consisted of nine respondents received the drug Simvastatin 10 mg and avocado meat weighing 330 grams were consumed 1x / day. The control group consisted of 11 respondents have a drug Simvastatin 10 mg oral 1x daily at night before bed. All groups examined total cholesterol levels before treatment and after treatment on day 15. Result: The results of one-way ANOVA test showed a significant difference between before and after treatment in the first group (p=0,001) and second group (p= 0,005), and there is no significant difference before and after treatment in the control group (p= 0,248). The difference between the three groups showed p= 0.025. Conclusion: The conclusion of this study is giving rosella tea and avocado has the same effectiveness in lowering total cholesterol levels so that health workers can suggest the use of rosella tea and avocado as a companion therapy to reduce total cholesterol level.

scholarly journals Relationship between Smoking and Concentrations of Some Biochemical Parameters in Blood of Young Smokers

2019 ◽  
pp. 65-70
Author(s):  
Khalid Shaalan Sahab
Keyword(s):  
Cigarette Smoking ◽  
Total Cholesterol ◽  
Biochemical Parameters ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Total Serum ◽  
Control Group ◽  
Low Density ◽  
Young Smokers

Cigarette smoking is main cause of lung cancer and one of the major causes for cardiovascular problems. Smoking causes changes to some blood contents. The study aimed to identifying the influence of cigarette smoking on concentrations of glucose, total cholesterol, triglycerides, high density lipoprotein (HDL-C), glucose, low density lipoprotein (LDL-C) and bilirubin in blood of young smokers. The study conducted among 30 subjects of young smokers and compared with 20 non-smoker volunteers as control group. The biochemical parameters of blood among all subjects have been determined by using available commercial kits. The results have been showed that the mean of the total serum bilirubin levels were lower in smokers when compared with controls but with significant (P > 0.05). Serum fasting blood glucose (FBG) and total cholesterol (TC) levels were found to be higher in smokers as compared to controls (P ≤ 0.05). The comparison between the estimated levels of triglycerides (TG) and low density lipoprotein-c (LDL-C) in smokers and healthy controls subjects showed no significant increased (P > 0.05). Also there are no significant decrease in levels of HDL-C in smoking subjects as compared to controls Conclusion: The conclusion from results, that smoke of cigarette can influence on biochemical parameters such as glucose, total cholesterol and bilirubin in early stages of smoking and produce a problems to cardiovascular system in early ages.

scholarly journals PENGARUH PEMBERIAN KEFIR SUSU SAPI TERHADAP KADAR KOLESTEROL TOTAL TIKUS JANTAN SPRAGUE DAWLEY

2012 ◽  
Vol 1 (1) ◽  
pp. 224-228
Author(s):  
Finta Wahdania ◽  
Adriyan Pramono
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Serum Cholesterol ◽  
Serum Cholesterol Level ◽  
Total Cholesterol Level ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Cholesterol Lowering

Background : A high serum cholesterol level might become one of contributing risk factor for development of cardiovascular disease (CVD). Serum cholesterol level could decreased by acid lactic bacteria (ALB) content. Kefir, one of probiotic product have been claimed as a cholesterol-lowering agent. The purpose of this research was to determine effect of cwo milk kefir to serum total cholesterol level in animal model. Method : true-experimental by using pre post test with randomized control group design. Twenty eight male Sprague dawley rat were divided into four group (K,P1, P2, P3) and induced by high cholesterol diet. Intervention started from 1,5 ml; 2 ml and 3 ml of kefir respectively to P1, P2 and P3 for fifteen days. Cholesterol level was examined by CHOD-PAP method. Data was examined by using one way ANOVA. Result : Total cholesterol level decreased significantly in all group but there is no defference between group. The highest reduction of total cholesterol was 31,45%, found in group P3 followed by 31,40% for K; 30,90% for P2 and 27,04 % for P1. Conclusion : kefir administration did not contribute in lowered total cholesterol level, result of this study did not support administration of kefir as a cholesterol-lowering agent

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