scholarly journals Authentication of Wistar rat fats with gas chromatography mass spectometry combined by chemometrics

10.5219/1229 ◽  
2020 ◽  
Vol 14 ◽  
pp. 52-57
Author(s):  
Any Guntarti ◽  
Ibnu Gholib Gandjar ◽  
Nadia Miftahul Jannah
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Methyl Ester ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Principal Component ◽  
Total Content ◽  
Wistar Rat ◽  
Animal Fats ◽  
Mass Spectometry

Indonesia is a country with the largest Muslim population in the world, which is very concerned about halal food. The most problem that’s very concerning nowadays was that food products were contaminated by unclean meat, such as rat meat. The purpose of this study was to authenticate rat fat using Gas Chromatography-Mass Spectrophotometry (GC-MS) combined with chemometrics. In this study, rat fat were heated in oven at 90 °C – 100 °C for approximately one hour until the oil came out. After that, the derivatization process was carried out to convert fat into methyl ester compounds using NaOCH3 and BF3. Methyl ester compound than injected into the GCMS instrument system. In addition to rat fat, other fat extraction were carried out, such as pigs, cows, chickens, wild boars, dogs, and goats. The combination of chemometrics Principal Component Analysis (PCA) was used to classify rat fat with other animal fat. Based on the results of the study showed that fatty acids in rats using GCMS produced 6 types of fatty acids, namely: myristat (0.15 ±0.09%), palmitoleate (0.73 ±0.54%), palmitate (19.08 ±3.54%), linoleate (30.14 ±16.90%), oleate (40.48 ±2.74%), and stearate (2.55 ±0.01%). Total content of rat fatty acids was 93.13%, with unsaturated fatty acids 71.35% and saturated fatty acids 21.78%. Chemometrics PCA from rat fat can be grouped with other animal fats

scholarly journals Chemometric Match to Evaluate Fatty Acids Degradation of Animal and Plant Fats after Heating Treatment by Principal Component Research (PCR)

2019 ◽  
Vol 8 (2S3) ◽  
pp. 325-330
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Principal Component ◽  
Heating Process ◽  
Heat Condition ◽  
Animal Fats ◽  
Edible Fats ◽  
Heating Treatment ◽  
Diet Intake

Fats have value demand of diet intake to supply energy and as cooking medium. Unsaturated fatty acids (UFAs) may prone to fatty acids (FAs) oxidation during heating process of fat by altering the double bonds between carbon atoms into trans fatty acids (TFAs) and saturated fatty acids (SFAs). TFAs and SFAs have been known as potential undesirable health effects for consumption. Thus monitoring the changes of oxidation UFAs at certain heat condition is essential to investigate the heat impact of various edible fats from animals and plants. The aim of the study was to evaluate FAs degradation various edible fats after heating treatments. The variety of fats such as chicken, beef, lard, mutton and plant fats were heated under controlled temperatures (120,180 and 240 ℃) and hours of heating (0.5, 1, 2 and 3 hrs). FAs were profiled by combination of gas chromatography-flame ionization detector (GC-FID) and chemometrics techniques. The major FAs were identified after heating treatment such as Palmitic (C16:0), Stearic (C18:0), Elaidic (C18:1n9t), Oleic (C18:1n9c) and Linolelaidic (C18:2n6c). Observation through sum heat at 120℃ /3hrs, 180℃/ 3hrs and 240℃/ 2 and 3hr contributed by SFAs and TFAs (C16:0, C18:0 and C18:1n9t) for all animal fats. The UFAs (C18:1n9c and C18:2n6c) contributed by sum heat of 120℃/ 0.5-2hrs, 180 ℃/ 0.5-2hrs and 240℃/0.5 in a group. Plant fats were groups together by themselves and segregated from animal fats. These findings suggested that the degradation of UFAs to the SFAs and TFAs are related to the heat condition regardless species of animal fats

scholarly journals Authentication of Dog Fat With Gas Chromatography-Mass Spectroscopy Combined With Chemometrics

2018 ◽  
Vol 10 (4) ◽  
pp. 124
Author(s):  
Any Guntarti
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Methyl Ester ◽  
Mass Spectroscopy ◽  
Saturated Fatty Acids ◽  
Total Content ◽  
Component Analysis ◽  
Muslim Population ◽  
Muslim Majority ◽  
Gas Chromatography Mass Spectroscopy

Indonesia is a country with a majority of Muslim population. As a Muslim majority country, providing halal products becomes a liability. The problem that has received a lot of attention right now is the concern that contamination of food products by meat, one of them is dog meat. The purpose of this study was to authenticate dog fat by using Gas Chromatography-Mass Spectrophotometry (GC-MS)combined with chemometrics. Dogs that used in the study were taken from Bantul, Yogyakarta. Dog snacks were heated in an oven at 90-100ᵒC for approximately one hour. Oils / fats obtained from derivatization process was carried out by using NaOCH3 and BF3. The methyl ester compound was injected into the GC-MS instrument system. The Results of this study was dog fat that analyzed by GC-MS contains 9 types of fatty acids, namely: lauric (1.19 ± 0.25)%, myristate (4.33 ± 0.30)%, pentadekanoate (0.12 ± 0.02)%, palmitoleate (4.60 ± 0.07)%, palmitate (12.80 ± 2.90)%, margarate (0.13 ± 0.09)%, oleate (44 , 33 ± 5.22)%, stearic (14.71 ± 0.32)%, and arachidonic (1.29 ± 0.11)%. Total content of fatty acids in dogs was 50.22% and saturated fatty acids were 33.03%. Chemometric grouping with the Principles Component Analysis (PCA) shows that dog fat is very close to lard. The fatty acids that contained in dog fat can be used to authenticate dog meat.

scholarly journals Impact of Drying Method on the Evaluation of Fatty Acids and Their Derived Volatile Compounds in ‘Thompson Seedless’ Raisins

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 608 ◽  
Author(s):  
Dong Wang ◽  
Hafiz Umer Javed ◽  
Ying Shi ◽  
Safina Naz ◽  
Sajid Ali ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Principal Component Analysis ◽  
Volatile Compounds ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Principal Component ◽  
Heptanoic Acid ◽  
Drying Method ◽  
Thompson Seedless ◽  
Air Drying

Air- and sun-dried raisins from Thompson Seedless (TS) grapes were analyzed under GC/MS to evaluate fatty acids (FAs) and their derived volatile compounds, coming from unsaturated fatty acids oxidation. A total of 16 FAs were identified in TS raisins, including 10 saturated fatty acids (SFAs) and 6 unsaturated fatty acids (USFAs). The contents of C18:0, C15:0, and C16:0 among SFAs and C18:3, C18:2 and C18:1 in USFAs were significantly higher. Furthermore, USFAs such as C16:1 and C20:1 were only identified in air-dried raisins. The principal component analysis showed the increased content of FAs and FA-derived compounds were in air-dried and sun-dried raisins, respectively. Among FA-derived compounds, 2-pentyl furan, 3-octen-2-one, 1-hexanol and heptanoic acid were more potent. This study shows that air-drying is more favorable for the production of fatty acids (SFAs and USFAs), whereas sun-drying is more advantageous in terms of fatty acid-derived volatiles.

Effect of animal age and gender on fatty acid and elemental composition in Austrian beef applicable for authentication purposes

2013 ◽  
Vol 67 (3) ◽  
Author(s):  
Adriána Bednárová ◽  
Ján Mocák ◽  
Walter Gössler ◽  
Margit Velik ◽  
Josef Kaufmann ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Elemental Composition ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Fatty Acid Content ◽  
Principal Component ◽  
Omega 3 ◽  
Age And Gender ◽  
And Gender

AbstractThe main aim of the present study was to find differences in the content of fatty acids and variations in elemental composition in beef samples of longissimus dorsi muscle related to cattle age and gender. A further goal was to describe interrelations among the selected variables (descriptors) characterising the samples. For this purpose, an extensive data table was compiled, which contains chemical descriptors specifying forty-six beef samples originating from four well-known Austrian grassland-based beef labels. The following descriptors were investigated: (a) concentrations of 33 fatty acids, (b) concentrations of 19 elements, (c) contents of dry-mass, protein, intramuscular fat, and ash, (d) total content of saturated fatty acids (SFA), mono-unsaturated fatty acids (MUFA), and poly-unsaturated fatty acids (PUFA), (e) total contents of omega-3 (n-3) and omega-6 (n-6) PUFA and their ratio. The correlation analysis provided a number of statistically significant correlations among the descriptors, which were concordant with the results of the principal component analysis and cluster analysis. Furthermore, the effect of age and gender of cattle (both acting as target factors) on the fatty acid content and elemental composition of beef was examined by analysis of variance (ANOVA) and appropriate non-parametric tests. Several important interrelations among the beef characteristics investigated were also discovered. Finally, the most relevant beef descriptors were utilised in linear discrimination analysis (LDA) for predicting the slaughter age of the cattle for beef authentication.

scholarly journals Profil Asam Lemak Gonad Lima Spesies Landak Laut (Echinoidea) Dari Pantai Selatan Kabupaten Gunung Kidul Daerah Istimewa Yogyakarta

2014 ◽  
Vol 19 (1) ◽  
Author(s):  
Sri Endang Purnami ◽  
Trijoko ◽  
Raras Toeti Pratiwi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Sea Urchin ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Economic Value ◽  
Acid Methyl Ester ◽  
Acid Methyl

AbstractSea urchin (Echinoidea) is an avertebrate animal whose habitat can be found from the intertidal to shallow subtidal areas. Sea urchin has a very important role in reef ecology, especially in intertidal and subtidal areas. Sea urchin gonad also can be consumed and has high economic value. The aim of this study was to determine the profile of fatty acid Sea urchin gonad in South Coral in Gunung Kidul (Daerah Istimewa Yogokarta) Gonad samples were taken from two sampling locations, those were four species from Sepanjang beach and one species from Wediombo coast. Fat was separated from the gonad using the method of Blight and Dyer (1959) and fatty acid methyl ester were prepared by direct transesterification reaction according to Morisson and Smith’s method (1964). Fatty acid methyl ester were separated and analysed by gas chromatography. The result showed that there are 10 types of fatty acid found in sea urchin gonad belonging to saturated and unsaturated fatty acid both MUFA (monounsaturated fatty acid) dan PUFA (polyunsaturated fatty acid). In all samples. The level of saturated fatty acids is higher than the unsaturated fatty acids, especially myristic (C14:0) and palmitic acid (C16:0).Keywords: Fatty acid, Sea Uechin, South Coral Gunung Kidul Daerah Istimewa YogyakartaAbstrakLandak laut (Echinodea) merupakan hewan avertebrata yang banyak dijumpai pada daerah pasang surut yang berbatu dan berpasir. Landak laut memiliki peranan yang sangat penting pada ekologi karang terutama di daerah pasang surut, selain itu gonadnya juga dapat dikonsumsi dan memiliki nilai ekonomi yang tinggi. Tujuan dari penelitian ini mengetahui keragaman spesies dan komposisi asam lemak gonad Landak laut yang ada di Pantai Selatan Kabupaten Gunung Kidul. Sample gonad diambil dari 2 lokasi sampling yaitu empat jenis dari Pantai Sepanjang dan satu jenis dari Pantai Wediombo. Lemak dipisahkan dari gonad menggunakan metode Blight and Dyer (1959) dan dimetilasi melalui metode Morison and Smith (1964). Kadar asam lemak gonad kemudian dianalisa dengan GC. Hasil analisa asam lemak sampel gonad ditemukan Asam lemak jenuh yang meliputi asam kaprilat (C8:0), asam miristat (C14:0), asam palmitat (C16:0), asam stearat (C18:0) dan asam arakhidat (C20:0). Asam lemak tidak jenuh antara lain asam oleat (C18:1n-9), asam palmitoleat (C16:1n-7), linoleat (C18:2n-8) dan asam eicosapentaenoat (C20: 5n-3). Hasil analisa menunjukkan bahwa kadar asam lemak jenuh lebih tinggi dibanding asam lemak tidak jenuh terutama asam miristat sebesar 27,20% dan palmitat 24,44% sedangkan asam lemak tak jenuh yang tinggi adalah asam Eicosapentaenoat sebesar 14,83%, keduanya ditemukan pada Colobocentrotus sp.2. Jenis Landak laut di Pantai Selatan Kabupaten Gunung Kidul sangat beragam sedangkan jenis asam lemak yang terkandung pada lima sampel gonadnya sama tetapi berbeda kadarnya.Kata kunci: Asam lemak, landak laut, karang Gunung Kidul Daerah Istimewa Yogyakarta

Characterization of Biodiesel from Vegetable Oil Using Comprehensive Two-Dimensional Gas Chromatography

2012 ◽  
Vol 260-261 ◽  
pp. 312-317 ◽  
Author(s):  
Ferdinando Corriere ◽  
Giorgia Peri ◽  
Vincenzo La Rocca
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Internal Combustion Engines ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Combustion Engines ◽  
Two Dimensional ◽  
Mass Percentage ◽  
Heating Value

Biodiesel from vegetable oils has been analyzed and its suitability for internal combustion engines has been assessed. The Standard biofuel’s characteristics have been investigated. Gas chromatography (GC) tests were carried out to characterize the fatty acid behavior. It was found that the mass percentage of saturated fatty acids did not change substantially with time, while unsaturated fatty acids increased with time for both B100 and B30. An increase in density for B100 and B30 with time was noted. Biodiesel density was within the standardization. The calorific values of biodiesel and its blend increased with time. Higher heating value for B100 increased from 41 to 43 MJ/kg while B30 increased from 44 to 45 MJ/kg.

scholarly journals ВМІСТ ЖИРНИХ КИСЛОТ В ЛІПІДАХ ФЕТАЛЬНИХ СТОВБУРОВИХ КЛІТИН КОТА

2016 ◽  
Vol 18 (3(70)) ◽  
pp. 136-141
Author(s):  
L.V. Kladnitskaya ◽  
A.J. Mazurkiewicz ◽  
V.V. Danchuk ◽  
S.V. Velichko ◽  
S.V. Midyk
Keyword(s):  
Fatty Acids ◽  
Stem Cells ◽  
Unsaturated Fatty Acids ◽  
Methyl Esters ◽  
Saturated Fatty Acids ◽  
Fatty Acid Content ◽  
Total Content ◽  
Primary Material ◽  
Fetal Stem Cells ◽  
Index Value

Defined content of fatty acids in lipids cat stem cells derived from fetal primary material. Fetal stem cells (FSCs) cat treated by culturing primary material in the CO2 incubator containing 5% CO2, at a temperature 37°С in DMEM medium with the addition of 15 – 20% fetal bulls serum and 1% antibiotic–antimycotic. When confluent monolayer reached 70 – 80%, the cells are removed from the culture dishes and held subcultivation to reduce the heterogeneity of culture. The resulting stem cells are tested for fatty acid content by gas–liquid chromatography.Determination of fatty acids in lipids fetal stem cells conducted under SOST ISO 5508–2001. Sample preparation was performed according to ISO 150 5509–2002 in our modification. A mixture of methyl esters of fatty acids were analyzed on the gas chromatograph Trace GC Ultra with flame ionization detector for capillary column SPTM –2560, 100 m x 0.25 mm ID, 0.20 μm film (Supelco). Identification of fatty acids was performed using a standard sample Supelco 37 Сomponent FAME Mix. Performed quantitative assessment by spectrum of crystal planes valuation peaks methylated derivatives LCD and determine their content as a percentage of the total content of all the LCD.Investigated that the lipids contained cat FSCs short–, medium– and long–chain fatty acids. In the lipid fetal stem cells found cat 18 Number fatty acids from saturated – most of palmitatе (34.53 ± 0.58%), with monounsaturated – оleate (20.20 ± 0.93%), with polyunsaturated – linoleic acid (6.27 ± 0.01%). Least composed of lipids of cells found cis–8.11.14–eykozatriyenovoyi acid (0.03 ± 0.01%).The total content of saturated fatty acids in the lipid cat FSCs is 67.75, unsaturated fatty acids – 32.25%. Saturation ratio is 2.10. Monoyenic fatty acids identified in the number of 23.19%, and polyenic – 9,06%. The index value n3 fatty acids to n6 in lipids cat FSCs is 0.35. 

scholarly journals The Study of Variation in Fatty Acids Composition for Animal Fats

1970 ◽  
Vol 66 (2) ◽  
Author(s):  
Flavia POP ◽  
Cornel LASLO
Keyword(s):  
Fatty Acids ◽  
Chemical Composition ◽  
Polyunsaturated Fatty Acids ◽  
Milk Fat ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Cow Milk ◽  
Animal Fats ◽  
Poultry Fat

In this article the chemical composition of 2 types of animal fats (cow milk fat and poultry fat) following the variation of saturated and unsaturated fatty acids proportion during freezing storage was studied. Determination of chemical composition of animal fats is important in establishing organoleptic and physico-chemical parameters, the variation of them in time, nature and proportion of fatty acids conferring specific characteristics to them. For milk fat was determined the following chemical composition: saturated fatty acids 68.35%, monounsaturated fatty acids 29.25%, polyunsaturated fatty acids 2.4%. After 4 months of storage under freezing there was a change in fatty acids proportion, saturated fatty acid content increased to 70.41%, monounsaturated fatty acids content decreased to 28.23%, and polyunsaturated fatty acids content decreased to 1.35% due to oxidation process when decreased the degree of unsaturation due to unsaturated fatty acids oxidation. In the case of poultry fat there was also an increase of saturated fatty acids (30.71%) and a decrease for monounsaturated (43.47%) and polyunsaturated (24.81%) fatty acids content.

scholarly journals Gas chromatography-mass spectrometry in the taxonomy of Miscanthus

2020 ◽  
Vol 23 (8) ◽  
pp. 1076-1081
Author(s):  
N. M. Slynko ◽  
N. V. Burmakina ◽  
O. M. Potseluyev ◽  
S. Yu. Kapustyanchik ◽  
G. Yu. Galitsin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Russian Far East ◽  
Far East ◽  
Principal Component ◽  
Total Content ◽  
Gas Chromatography Mass Spectrometry ◽  
Miscanthus Sinensis ◽  
Chromatography Mass Spectrometry

Chemotaxonomy as a system approach deals with intra- and interspecific polymorphism of a group of taxa in order to clarify their taxonomic positions or to select material for selection or introduction. In this study we performed chemotaxonomic analysis of specimens of Miscanthus sinensis and M. sacchariflorus collected in the Russian Far East and of hybrid plants of both natural and artificial origin. We found 153 substances and identified 143 of them in extracts of eleven Miscanthus plants by gas chromatography-mass spectrometry (GC-MS). These substances can be grouped into alkanes (20 compounds), fatty acids (34), phenols (13), sterols (18) toсopherols (8), norterpenoids (12), and phytols (13), as well as their derivatives. The main components of the extracts of miscanthus samples are fatty acids and their derivatives (total content 19.94–41.02 %), dominated by palmitic and linolenic acids, and sterols (mainly β-sitosterol, stigmasterol, and α-amyrin), which constitute 17.15–31.73 %. The values of the CPI “oddness index” for the alkane components of the extracts were within 1.55–7.18, with extracts from leaves of the Far Eastern samples characterized by the lower half of this range (1.55–2.74), while extracts from leaves of hybrids fell to the upper half (5.78–7.18). Principal component analysis of extraction profiles allowed us to separate three distinct clusters: M. sinensis, M. sachariflorus, and their hybrids, as well as to verify the origin of one of the natural hybrids. The results of chemotaxonomic analysis mostly matched those of DNA sequencing of a fragment of the plastid genome, which, moreover, allowed us to identify the species nature of the maternal plants used to obtain these hybrids. Chemotaxonomic analysis using GC-MS was found to be an efficient additional technique to delimit various morphological forms of M. sinensis, M. sachariflorus, and their hybrids.

scholarly journals Quantification of Seed Oil Content and Fatty Acid Profile of Jatropha cucas L. from Guizhou, China

2016 ◽  
Vol 8 (2) ◽  
pp. 92
Author(s):  
Hamidou SENOU ◽  
Cai X. ZHENG ◽  
Gabriel SAMAKE ◽  
Mamadou B. TRAORE ◽  
Fousseni FOLEGA ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Jatropha Curcas ◽  
Unsaturated Fatty Acids ◽  
Methyl Esters ◽  
Saturated Fatty Acids ◽  
Total Content ◽  
Biodiesel Production ◽  
Seed Oils ◽  
Fatty Acids Composition

<p class="1Body">The methyl esters of fatty acids composition of the oil from <em>jatropha curcas</em> seeds were analyzed by gas chromatography-mass spectrometer GC-MS. Fourteen components were found to be representative with 99.52% of the total content of seed oils. The main constituents were unsaturated fatty acids (71.93%) and saturated fatty acids (27.59%). For the saturated fatty acids composition such as palmitic and stearic acid, the rate was 15.80% and 10.79%, respectively. Linoleic acid (39.58%) and oleic acid (30.41%) were obtained in highest concentration among the unsaturated fatty acids identified in the seeds oil of <em>Jatropha curcas</em> from Guizhou. This value also justifies the fluidity of the oil at room temperature. A high percentage of polyunsaturated fatty acids (39.58%) and a slightly lower rate of monounsaturated fatty acids (32.35%) were also observed. The seed oils profile of Guizhou <em>Jatropha curcas</em> presents the desirable fatty acid C14 to C18 and interesting features for the biodiesel production.</p>

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