scholarly journals The study correlation between physicochemical properties, botanical origin and microbial contamination of honey from the south of Ukraine

10.5219/1179 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 863-869
Author(s):  
Oleksandra Berhilevych ◽  
Victoria Kasianchuk ◽  
Mykola Kukhtyn ◽  
Lubov Dimitrijevich ◽  
Tatyana Marenkova
Keyword(s):  
Physicochemical Properties ◽  
Bacillus Cereus ◽  
Sugar Content ◽  
First Grade ◽  
Dilution Method ◽  
Botanical Origin ◽  
Microbial Composition ◽  
Honey Production ◽  
Fodder Crops ◽  
The Relationship

The honey production is one of the most important agriculture branches of Ukraine. Since the beginning of 2000 year, honey becomes one of the first products of agriculture production that was allowed to export from Ukraine to the EU countries. The present study aims to evaluate microbial composition of honey of higher and first grades from south of Ukraine, which differ in water, sugar content and acidity. The correlation beetween physicochemical properties, botanical origin and microbial composition of honey were investigated. Total aerobic count (TAC), total count of fungi (TFC) and yeast (TYC), number of sporeforming anaerobes (SFA), Bacillus cereus and Clostridium botulinus were determined. Plate dilution method with specific cultural mediums and incubation conditions was used for indication and identification of microorganisms as well for quantitative CFU count determination in 1g of honey. The results indicated that the value of TAC was from 1.4 ±0.1 x 102 to 3.7 ±0.5 x 102 CFU.g-1 in higher grade honey and from 2.1 ±0.3 x 102 to 4.6 ±0.4 x 102 CFU.g-1 in first grade honey. Average amounts of fungi and yest in the first grade honey were more than in higher grade honey and amounted 15.±0.9 CFU.g-1, 6.48 ±0.7 CFU.g-1 and 17.98 ±1.2 CFU.g-1, 8.04 ±0.8 CFU.g-1 respectively. Also it was found that, 25.8% and 37.1% of higher and first grade honey samples had sporeforming anaerobes. The more of samples with Bacillus cereus and Clostridium botulinum were among samples of first-grade honey samples. In addition, we have determined that, samples of buckwheat honey and honey from fodder crops of both grades were more contaminated with all microorganisms than samples of sunflower honey and honey from fruit and non-fruit trees.Results of this work has revealed the relationship between the number of different groups and species of microorganisms in higher and first grade honey from south of Ukraine and its physicochemical properties and botanical origin. Our research could help to substantiate the sources of contamination of honey and confirm the existing findings of scientists about the direct proportional dependence of the content of microorganisms in honey, depending on its moisture content, acidity and sugars.

scholarly journals Correlating Capsaicinoid Levels and Physicochemical Proper-ties of Kimchi and Its Perceived Spiciness

Foods ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 86
Author(s):  
So-Ra Yoon ◽  
Yun-Mi Dang ◽  
Su-Yeon Kim ◽  
Su-Yeon You ◽  
Mina K. Kim ◽  
...  
Keyword(s):  
Correlation Analysis ◽  
Physicochemical Properties ◽  
Correlation Coefficient ◽  
Partial Correlation ◽  
Sugar Content ◽  
Amino Acid Content ◽  
Titratable Acidity ◽  
Free Sugar ◽  
Partial Correlation Analysis ◽  
The Relationship

Capsaicinoid content, among other factors, affects the perception of spiciness of commercial kimchi. Here, we investigated whether the physicochemical properties of kimchi affect the spicy taste of capsaicinoids perceived by the tasting. High-performance liquid chromatography (HPLC) was used to evaluate the capsaicinoid content (mg/kg) of thirteen types of commercial kimchi. The physicochemical properties such as pH, titratable acidity, salinity, free sugar content, and free amino acid content were evaluated, and the spicy strength grade was determined by selected panel to analyze the correlation between these properties. Panels were trained for 48 h prior to actual evaluation by panel leaders trained for over 1000 h according to the SpectrumTM method. Partial correlation analysis was performed to examine other candidate parameters that interfere with the sensory evaluation of spiciness and capsaicinoid content. To express the specific variance after eliminating the effects of other variables, partial correlations were used to estimate the relationships between two variables. We observed a strong correlation between spiciness intensity ratings and capsaicinoid content, with a Pearson’s correlation coefficient of 0.78 at p ≤ 0.001. However, other specific variables may have influenced the relationship between spiciness intensity and total capsaicinoid content. Partial correlation analysis indicated that the free sugar content most strongly affected the relationship between spiciness intensity and capsaicinoid content, showing the largest first-order partial correlation coefficient (rxy/z: 0.091, p ≤ 0.01).

scholarly journals Assessment of the Botanical Origin of Polish Honeys Based on Physicochemical Properties and Bioactive Components with Chemometric Analysis

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4801
Author(s):  
Maria Tarapatskyy ◽  
Patrycja Sowa ◽  
Grzegorz Zaguła ◽  
Małgorzata Dżugan ◽  
Czesław Puchalski
Keyword(s):  
Comparative Analysis ◽  
Chemical Composition ◽  
Physicochemical Properties ◽  
Phenolic Acids ◽  
Pollen Analysis ◽  
Sugar Content ◽  
Proline Content ◽  
Botanical Origin ◽  
Bioactive Components ◽  
Bioactive Substances

Is it possible to characterize the types of honey based on their chemical composition, their content of bioactive substances, and their physicochemical properties? The objective of this study was a comparative analysis of four types of honey from the Carpathian Foothills area, located in south-east Poland, based on the content of the main phenolic acids and proline, the mineral composition, and selected physicochemical properties. Most analyses, such as those of phenolic acids, sugars, and proline content, in honey samples were performed using chromatographic methods. These experiments demonstrated that honeydew honeys were the richest in phenolic acids, minerals, as well as oligosaccharides, compared to other honeys. Dark-colored honeys were characterized by the highest proline content. The dominant elements in all types of honey were potassium and calcium. The results of the present study show that analyses of specific phenolic acids, minerals, proline, and sugar content, in combination with chemometrics analysis, may successfully differentiate between the biological origins of honey samples and allow the preliminary verification of the samples before performing time-consuming pollen analysis.

Unpacking the Relationship Between Preschool Social Skills and First Grade Academic Competence

2008 ◽  
Author(s):  
Brittany L. Rhoades ◽  
Heather Kiernan Warren ◽  
Mark T. Greenberg ◽  
Celene E. Domitrovich
Keyword(s):  
Social Skills ◽  
First Grade ◽  
Academic Competence ◽  
The Relationship

Biologically Important Physicochemical Properties of Some Cephalosporins

1992 ◽  
Vol 57 (8) ◽  
pp. 1739-1746
Author(s):  
Katarína Škvareninová ◽  
Štefan Baláž ◽  
Ernest Šturdík ◽  
Miroslav Veverka ◽  
Jana Adamcová ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Partition Coefficients ◽  
Dissociation Constants ◽  
Transport Rate ◽  
Hydrolysis Rate ◽  
Antibacterial Effects ◽  
Oh Groups ◽  
Non Linear Equation ◽  
Cell Components ◽  
The Relationship

In the series of cephalosporin derivatives, consisting of eight 7-(R1-CH2-CO-NH)cephalosporanic acids and of seven analogical compounds with 3-acetoxymethyl replaced by 3-CH3, physicochemical properties, which are expected to play a role in their antibacterial effects (the transport rate parameters and partition coefficients in the systems 1-octanol-water and 1-octanol-buffer, dissociation constants of the 4-carboxyl group, reactivity towards L-glutathione imitating the nucleophilic groups of the cell components and hydrolysis rate parameters), were determined. Linear dependences were observed between the partition coefficients and the π-constants of the varying substituents as well as between reactivity towards SH-groups of L-glutathione and OH-groups. The relationship between the transport rate parameters and partition coefficients, both measured in buffered as well as non-buffered system, was described by a common non-linear equation.

scholarly journals An exploration of the relationship between recruitment communication and foraging in stingless bees

2021 ◽  
Author(s):  
Robbie I’Anson Price ◽  
Francisca Segers ◽  
Amelia Berger ◽  
Fabio S Nascimento ◽  
Christoph Grüter
Keyword(s):  
Stingless Bees ◽  
Sugar Content ◽  
Social Information ◽  
Food Resources ◽  
Food Sources ◽  
Foraging Strategies ◽  
High Quality ◽  
Recruitment Communication ◽  
Eusocial Bees ◽  
The Relationship

Abstract Social information is widely used in the animal kingdom and can be highly adaptive. In social insects, foragers can use social information to find food, avoid danger or choose a new nest site. Copying others allows individuals to obtain information without having to sample the environment. When foragers communicate information they will often only advertise high quality food sources, thereby filtering out less adaptive information. Stingless bees, a large pantropical group of highly eusocial bees, face intense inter- and intra-specific competition for limited resources, yet display disparate foraging strategies. Within the same environment there are species that communicate the location of food resources to nest-mates and species that do not. Our current understanding of why some species communicate foraging sites while others do not is limited. Studying freely foraging colonies of several co-existing stingless bee species in Brazil, we investigated if recruitment to specific food locations is linked to (1) the sugar content of forage, (2) the duration of foraging trips and (3) the variation in activity of a colony from one day to another and the variation in activity in a species over a day. We found that, contrary to our expectations, species with recruitment communication did not return with higher quality forage than species that do not recruit nestmates. Furthermore, foragers from recruiting species did not have shorter foraging trip durations than those from weakly-recruiting species. Given the intense inter- and intraspecific competition for resources in these environments, it may be that recruiting species favour food resources that can be monopolised by the colony rather than food sources that offer high-quality rewards.

scholarly journals Relationships Linking the Colour and Elemental Concentrations of Blossom Honeys with Their Antioxidant Activity: A Chemometric Approach

Agriculture ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 702
Author(s):  
Monika Kędzierska-Matysek ◽  
Anna Teter ◽  
Małgorzata Stryjecka ◽  
Piotr Skałecki ◽  
Piotr Domaradzki ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Physicochemical Properties ◽  
Black Locust ◽  
Antioxidant Properties ◽  
Principal Component ◽  
Botanical Origin ◽  
Antioxidant Parameters ◽  
Colour Parameters ◽  
Hue Angle ◽  
Chemometric Approach

The antioxidant activity of honey depends on the botanical origin, which also determines their physicochemical properties. In this study, a multivariate analysis was used to confirm potential relationships between the antioxidant properties and colour parameters, as well as the content of seven elements in five types of artisanal honey (rapeseed, buckwheat, linden, black locust, and multifloral). The type of honey was found to significantly influence most of its physicochemical properties, colour parameters, and the content of potassium, manganese and copper. Antioxidant parameters were shown to be significantly positively correlated with redness and concentrations of copper and manganese, but negatively correlated with the hue angle and lightness. The principal component analysis confirmed that the darkest buckwheat honey had the highest antioxidant activity in combination with its specific colour parameters and content of antioxidant minerals (manganese, copper and zinc). The level of these parameters can be potentially used for the identification of buckwheat honey.

scholarly journals Dissecting the Microbial Community Structure of Internal Organs During the Early Postmortem Period in a Murine Corpse Model

2021 ◽  
Author(s):  
Ruina Liu ◽  
Kai Zhang ◽  
Huan Li ◽  
Qinru Sun ◽  
Xin Wei ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
The Body ◽  
Time Since Death ◽  
Limited Information ◽  
Microbial Composition ◽  
Internal Organs ◽  
Organ Specific ◽  
Forensic Microbiology ◽  
The Relationship ◽  
Early Postmortem

Abstract Background Microorganisms inhabit and proliferate throughout the body both externally and internally, which are the primary mediators of putrefaction after death. However, limited information is available about the changes in the postmortem microbiota of extraintestinal body sites in the early decomposition stage of mammalian corpses. Results This study applied 16S rRNA barcoding to investigate microbial composition variations among different organs and the relationship between microbial communities and time since death over 1 day of decomposition. During 1 day of decomposition, Agrobacterium, Prevotella, Bacillus, and Turicibacter were regarded as time-relevant genera in internal organs at different timepoints. Pathways associated with lipid, amino acid, carbohydrate and terpenoid and polyketide metabolism were significantly enriched at 8 hours than that at 0.5 or 4 hours. The microbiome compositions and postmortem metabolic pathways differed by time since death, and more importantly, these alterations were organ specific. Conclusion The dominant microbes differed by organ, while they tended toward similarity as decomposition progressed. The observed thanatomicrobiome variation by body site provides new knowledge into decomposition ecology and forensic microbiology. Additionally, the microbes detected at 0.5 hours in internal organs may inform a new direction for organ transplantation.

scholarly journals Pengaruh Lokasi Geografis dan Vegetasi terhadap Sifat Fisikokimia Madu dari Ciwidey dan Bogor

2020 ◽  
Vol 1 (2) ◽  
pp. 71
Author(s):  
Emanuela Rae Alodia ◽  
Anas Bunyamin ◽  
Efri Mardawati
Keyword(s):  
Physicochemical Properties ◽  
Water Content ◽  
Sugar Content ◽  
Geographical Location ◽  
B Value ◽  
Reducing Sugar ◽  
National Standard ◽  
Color Analysis ◽  
A Value ◽  
C Value

<p><em>Physicochemical properties are one of the parameters of honey quality that impacts the sales of honey as well as the economy condition of the society in Ciwidey and Bogor. The physicochemical properties of honey are significantly influenced by the origin of the honey, including its geographical location and vegetation. This study aims to determine the physicochemical properties between multiflora honey from Ciwidey and Bogor with different source of nectar where Ciwidey honey is dominated by calliandra nectar while Bogor honey is dominated by kapok nectar. Data were analyzed with descriptive and quantitative method using independent t test for data with normal distribution (water content and reducing sugar content). Data that were not normally distributed (color analysis and acidity value) were tested non-parametrically with the Mann Whitney-U. The results showed that Bogor honey has the better physicochemical properties and meets Indonesian National Standard (Standar Nasional Indonesia, SNI) 8664:2018, with a water content of 15.15% w/w, L* values of 35.26, a* value of 28.66, b* value of 59.46, c* value of 66.01, h* value of 64.27°, reducing sugar content of 78.78% and acidity value of 35.56 mL NaOH kg-1. Phytochemical screening showed positive results on alkaloid, flavonoid, phenol and tannin, and the results of GC-MS analysis showed three significant compounds which are hydroxymethylfurfural of 48.26%, levoglucosan of 13.58% and ammonium carbamate of 9.18%.</em></p>

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