scholarly journals Hygienic quality and composition of raw sheep's bulk milk samples on selected Slovak farms during year 2018

10.5219/1058 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 359-362
Author(s):  
Martina Vršková ◽  
Vladimír Tančin ◽  
Michal Uhrinčať ◽  
Lucia Mačuhová ◽  
Kristína Tvarožková
Keyword(s):  
Slovak Republic ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
Important Species ◽  
Psychrotrophic Bacteria ◽  
Average Value ◽  
Milk Samples ◽  
Bulk Milk ◽  
Microbiological Criterion ◽  
Ewe's Milk

At the control of raw ewe's milk (REM) quality is a major microbiological criterion to the total bacterial count (TBC). The aim of our work was to determine the incidence of technologically important species of microorganisms in REM in Slovak Republic. At the monitored 28 ewe´s farms, we took bulk milk samples from evening or morning milking in spring, summer and autumn during year 2018. We analyzed nutrients (fat, protein, lactose and urea) and somatic cell count (SCC). We established technologically important microorganisms (MO) of psychotrophic MO, coliform MO, thermoresistant MO, spore-forming anaerobic MO. We have found a gradual increase in milk components, except for lactose, which is apparently related to the increasing cont of somatic cells during the milking period. We found that the TBC in raw sheep's milk complied an average of 132 x 103 CFU.mL-1 per spring (min 34 x 103 CFU.mL-1, max 501 x 103 CFU.mL-1), 300 x 103 CFU.mL-1 in summer (min. 31 x 103 CFU.mL-1, max 640 x 103 CFU.mL-1) and in autumn with an average value of 147 x 103 CFU.mL-1 (min 52 x 103 CFU.mL-1, max 276 x 103 CFU.mL-1). The enormous occurrence of psychrotrophic bacteria was found in one farm in northern Slovakia during spring and summer, in the summer we increased our number to 3 farms, in the autumn of 2 farms. At the other farms we evaluated the average value of 12 x 103 CFU.mL-1 per spring and 28 x 103 CFU.mL-1 in summer, 130.5 x 103 CFU.mL-1 in the autumn. The count of thermoresistent MO achieved 57 CFU.mL-1 per spring, 15 CFU.mL-1 in summer and 33 CFU.mL-1 in the autumn. The presence of spore-forming anaerobic MO in raw ewe´s milk was found during spring at six farms out of 15, but in the summer at just one in 9, in the autumn two farms.

scholarly journals Evaluating the effect of storage conditions on milk microbiological quality and composition

2018 ◽  
Vol 57 (1) ◽  
pp. 52-62 ◽  
Author(s):  
L.F. Paludetti ◽  
K. Jordan ◽  
A.L. Kelly ◽  
D. Gleeson
Keyword(s):  
Enzyme Activity ◽  
Storage Time ◽  
Storage Temperature ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Storage Conditions ◽  
Total Bacterial Count ◽  
Bacterial Counts ◽  
Milk Samples ◽  
Bulk Milk

Abstract In this study, the effect of storage temperature (2 or 4°C) on the composition of milk and microbiological load was investigated over 96 h. Milk samples were collected from farm bulk milk tanks after one complete milking and stored at 2 or 4°C over 96 h. Total bacterial count (TBC), psychrotrophic bacterial count (PBC) and proteolytic bacterial count (PROT) were affected by storage time and temperature and varied significantly between farms (P < 0.05). The levels of TBC, PBC and PROT bacterial count increased from 4.37 to 6.15 log cfu/mL, 4.34 to 6.44 log cfu/mL and 3.72 to 4.81 log cfu/mL, respectively, when the milk was stored for 96 h at 2°C. The milk samples stored at 4°C had higher increases in these bacterial counts after 72 h in comparison to milk samples stored at 2°C. The casein fraction content was lower in milk samples stored at 4°C, which could be due to high levels of PROT bacteria or enzyme activity in these samples. Milk stored for 96 h at 2°C has less impact on composition or processability parameters compared to milk stored at 4°C.

Rapid Detection of Psychrotrophic Bacteria In Manufacturing Grade Raw Milks

1991 ◽  
Vol 54 (11) ◽  
pp. 861-867 ◽  
Author(s):  
S. R. TATINI ◽  
P. MEKALA ◽  
A. EL-HABAZ ◽  
M. W. GRIFFITHS
Keyword(s):  
Rapid Detection ◽  
Bacterial Count ◽  
Raw Milk ◽  
Plate Count ◽  
Psychrotrophic Bacteria ◽  
Milk Samples ◽  
Microscopic Count ◽  
Direct Epifluorescent Filter Technique ◽  
Native Microflora

Methods to rapidly assess the bacteriological quality of raw milk were investigated. Whereas direct microscopic count, modified psychrotrophic plate count, and direct epifluorescent filter technique (DEFT) did not correlate well with initial psychrotrophic bacterial count of raw milk, improvements were obtained after preincubation of the milk samples. The best preincubation conditions were identified as 30°C for 6 h, 21°C for 10 h, 13°C for 15 h, 13°C for 20 h, or 7°C for 37 h. The “square root” equation was applied to the data, and a model was produced for predicting growth of the native microflora of raw milk. Using this equation, a DEFT count after preincubation of the milk at 21°C for 10 h could accurately predict the initial psychrotroph count and the count after storage of the milk at 6°C for 48 h.

scholarly journals The Effect of Using Different Detergents in Cleaning Cows' Udders on The Microbial Content of Produced Milk

2018 ◽  
Vol 10 (3) ◽  
pp. 47
Author(s):  
Nizar Issa Alrabadi ◽  
Kamel I. Sultan
Keyword(s):  
Public Health ◽  
Bacterial Count ◽  
Total Coliform ◽  
Total Bacterial Count ◽  
Traditional Food ◽  
Gastrointestinal Infections ◽  
Microbial Counts ◽  
Milk Samples ◽  
Microbial Analysis ◽  
Northern City

This study investigated the effect of different detergents used to clean cows' udders on the microbial content of the produced milk using twenty cows in Ajloun, a northern city in Jordan. The milking process was repeated from same cows on three successive days. On day 1, we milked the cows after cleaning their udders using water only. This was repeated on the two successive days. Thereafter, the cows were milked after cleaning their udders by a different detergent each day. The process was also repeated for three successive days for each detergent. Microbial Analysis was carried out on the collected milk samples. The results indicated that cleaning cows' udders before milking has improved the hygiene conditions and reduced the total bacterial count, total coliform, staphylococci and enterococci spp counts and the values of yeast and molds. Different detergents had different effects on the microbial counts. Finally, the effectiveness of the detergent differed according to its brand. Our findings are important to public health because milk has been a traditional food and ironically a very potent carrier of gastrointestinal infections, if contaminated.

scholarly journals Assessment of Microbial Load in Water and Sediments of Rivers Otamiri and Nworie in Owerri, South Eastern Nigeria

2021 ◽  
pp. 27-39
Author(s):  
Okere J. Kelechi ◽  
Azorji J. Nnawuike ◽  
Iheagwam S. Kelechi ◽  
Emeka J. Emmanuel ◽  
Nzenwa P. Odinaka
Keyword(s):  
Drinking Water ◽  
Rainy Season ◽  
Bacterial Count ◽  
Total Coliform ◽  
World Health ◽  
Total Bacterial Count ◽  
E Coli ◽  
Average Value ◽  
Waste Dumps ◽  
Health Organization

Water pollution is a global problem. The study was carried out to assess the microbial constituents in water and sediment of Rivers Otamiri and Nworie during dry and rainy seasons (March and September, 2020) with comparison to WHO benchmark for drinking water .Samples were randomly collected at six (6) sampling points and analyzed using routine microbiological protocols.  The results revealed detectable amount of microbial activates in surface water of both rivers during the dry and rainy season. During the rainy season, a THB bioload average of 2.02x104 and 5.1x104CFU/mL for Nworie and Otamiri river respectively were measured. For the TCC, the average was 9.8x103 and 2.5x104 CFU/mL, while in the dry period there was corresponding reduction in the bioload value for both rivers. THB average value for Nworie river was 1.34x104 CFU/mL, with corresponding 3.5x104 values for Otamiri river, while in same inclination, TCC measured 6.1x103 and 1.8x104 CFU/mL. There were noteworthy variations in the values for the two rivers as well as in the two seasons. TBC value for Otamiri measured about 1.5 folds than the value for Nworie. In same trend, TCC value for Nworie measured about 39%. All values measured were above WHO permissible limit for drinking water. The biochemical and cultural features of the isolated microorganism in water showed the presence of E. coli identified in the entire stations (100%), Salmonella and faecal coliform occurred 83.3% each, while Vibrio and Shigella were detected in 4 of the 6 stations. The mean total bacterial count, total coliform count and total E. coli, were not in conformity with World Health Organization (WHO) Standard for drinking water and thus constitute a threat to the River; these were attributed to indiscriminate waste dumps around the rivers. The study underscores the need for adequate waste management system to forestall outbreak of pathogenic diseases in the area.

Proteolysis detection in milk: I. Interpretation of tyrosine value data for raw milk supplies in relation to natural variation, bacterial counts and other factors

1973 ◽  
Vol 40 (3) ◽  
pp. 371-381 ◽  
Author(s):  
H. S. Juffs
Keyword(s):  
Amino Acids ◽  
Trichloroacetic Acid ◽  
Natural Variation ◽  
Bacterial Count ◽  
Raw Milk ◽  
Atmospheric Temperature ◽  
Total Bacterial Count ◽  
Positive Relationships ◽  
Bacterial Counts ◽  
Bulk Milk

SummaryA method for detection of proteolysis in milk was evaluated. Amino acids and peptides soluble in trichloroacetic acid were estimated by the Lowry–Folin procedure with expression of results in terms of colour yield equivalent to that of tyrosine (the ‘tyrosine value’ (TV)). Some variables and alternatives in the method of estimation were investigated.TVs for milk freshly drawn from individual cows were extremely varied (0·31–0·92 mg/ml). TVs for samples of refrigerated milk from daily supply farms were in the range 0·40–0·58 mg/ml; no relationships between TV and bacterial counts were evident with these samples. With bulk refrigerated raw milk supplied to Brisbane for liquid consumption from country depots and local farms equipped with bulk vats, significant positive relationships were found between TV and total bacterial count (TBC) for supplies from some sources, but in no instance was a significant relationship found between TV and psychrotroph or proteolytic psychrotroph count. Significant positive relationships were found between TV of bulk milk supplies from some sources and atmospheric temperature, and between TBC of the supplies and atmospheric temperature. The significance of these various relationships for bulk milks is discussed.Natural variation in TV imposed limitations on reliability of the method to provide an index of proteolysis; in general the results indicated that its application was restricted to bulk milk cold-stored for at least 3 days.

scholarly journals Udder preparation and its effect on udder cleanliness and milk quality

2013 ◽  
Vol 12 (1) ◽  
pp. 128 ◽  
Author(s):  
A. K. AL- Hubaety
Keyword(s):  
Potassium Permanganate ◽  
Bacterial Count ◽  
Milk Quality ◽  
Hot Water ◽  
Total Bacterial Count ◽  
Milk Samples ◽  
Paper Towel ◽  
Before And After ◽  
Proportional Reduction

Total bacterial count was performed in eighty milk samples and eighty udder swabs collected from 20 cows, to compare the effect of treating udders with wet towel washing (55-60C0), followed by paper towel drying; three concentrations of Iodophre and Hibitane followed by paper towel drying and two concentrations of potassium permanganate followed by paper towel drying with non-treated udders .Results showed that Log10 of CFU/ swab of untreated udders was significantly (p≤ 0.05) differ from those swab samples when udder was treated with wet towel washing (55-60C0), followed by paper towel drying. By using iodophor, there was in asignificant (p≤ 0.05) reduction in the Log10 of CFU/ swab after udder treatment with all used concentrations of iodophor, and there was also proportional reduction in the Log10 CFU/ swab with each increase in the Iodophor concentration (0.1:100, 0.5:100 and 1:100) respectively. The same picture was gained by using Hibitane at concentrations of 0.25%, 0.5% and 1% as Iodophre. Potassium permanganate treatments with its two concentrations showed significant differences in Log10 /swab of udder before and after treatments and between its two concentrations 0.5:1000 and 1:1000.Total bacterial count of milk samples were a mirror of swab samples. From results it is evident that chemical disinfectants or hot water were effective in reducing udder contamination before milking.

Microbial Aspects of the Inuvik Sewage Lagoon

1986 ◽  
Vol 18 (2) ◽  
pp. 117-128 ◽  
Author(s):  
J. G. Henry ◽  
D. Prasad
Keyword(s):  
Bacterial Count ◽  
Total Alkalinity ◽  
Total Bacterial Count ◽  
Bacterial Number ◽  
Psychrotrophic Bacteria ◽  
Volatile Solids ◽  
Significant Difference ◽  
Order Of Magnitude ◽  
Plate Counts

A facultative sewage lagoon in Inuvik, N.W.T. was studied in August (ice free) and November (ice covered) 1984. Emphasis was placed on the microbiological aspects and their relation to lagoon performance. Analyses performed included, pH, total alkalinity, total solids, suspended solids, volatile solids, COD and BOD5 determinations. The microbiological tests included total plate counts (20°C) and psychrotrophic counts (1°C). Results indicated that the Inuvik sewage lagoon system achieved removal efficiencies, ranging from 90% BOD5 removal in summer to about 80% in winter. The total bacterial count was found to decrease in the liquid of the primary cells in the winter, but the bacterial count in the secondary cell was similar under both winter and summer conditions. No significant difference existed in bacterial counts in the sludge samples between primary and secondary cells. The total bacterial number decreased by one order of magnitude in the winter in sludges from all cells. A shift from mesophilic to psychrotrophic bacteria occurred in the winter. The percentage of psychrotrophs increased from less than 1% to over 80% with the change in season. The role of psychrotrophs in lagoon performance is discussed.

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