scholarly journals Mycotoxin-producing Penicillium spp. and other fungi isolated from grapes for wine production in Small Carpathians wine region

10.5219/1044 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 194-199
Author(s):  
Soňa Felšöciová ◽  
Miroslava Kačániová
Keyword(s):  
Relative Density ◽  
Penicillium Expansum ◽  
Penicillium Species ◽  
Chromatography Method ◽  
Wine Production ◽  
Identification Of Fungi ◽  
Wine Region ◽  
Layer Chromatography ◽  
Penicillium Spp ◽  
Exogenous Source

The diversity of mycobiota associated with grapevine in Vrbove, Slovakia at the harvest time in 2018 was evaluated. Fourteen samples of grapes were analyzed by plating methods and by plating methods with surface disinfection. The identification of fungi was performed using the morphological and microscopical characteristics. From the 1001 strains detected and identified from exogenous mycobiota, the most frequent genera were Alternaria, Rhizopus and Sordaria. Their relative density was low, except Alternaria. The most frequently encountered moulds and with the highest relative density from endogenous mycobiota were Alternaria, Cladosporium and Penicillium. Most of all genera had relative density less than 1%. Penicillium contributed small proportion in both sources. Penicillium citrinum was the most dominant species in exogenous and endogenous mycobiota. Penicillium expansum and P. glabrum were recorded in exogenous source and P. hordei, P. chrysogenum and P. griseofulvum in endogenous. Potentially toxigenic Penicillium species were tested for their toxigenic ability by thin layer chromatography method. Out of 15 tested isolates representing five potentially toxigenic species 11 produced at least one mycotoxin. Positive toxinogenity was detected in all tested strains of Penicillim citrinum (9/9).

scholarly journals Survey of mycobiota on Slovakian wine grapes from Small Carpathians wine region

10.5219/1322 ◽  
2020 ◽  
Vol 14 ◽  
pp. 721-728
Author(s):  
Soňa Felšöciová ◽  
Kačániová Miroslava ◽  
Vrábel František
Keyword(s):  
Cyclopiazonic Acid ◽  
Fungal Species ◽  
Grape Berry ◽  
Penicillium Expansum ◽  
Chromatography Method ◽  
Carpathian Region ◽  
Main Components ◽  
Wine Region ◽  
Layer Chromatography

A total of 13 samples of grapes (bunches) without apparent fungal contamination were analyzed. The samples were collected during the 2019 harvest from Vrbové village in the Small Carpathian region of Slovakia. For the isolation of fungi were used the direct plating technique on DRBC plates. The plates were incubated aerobically at 25 ±1 °C for one week in the dark. The data obtained from the cultivation of the grape berry samples revealed a high diversity of fungal species (a total of 1044 isolates were obtained). Alternaria and Rhizopus were the main components of the wine grape mycobiota of the Vrbovský subregion at harvest time (92%, each), followed by Cladosporium (85%), Penicillium (77%), Botrytis and Epicoccum (54%, each). The most abundant genera found by descending order were Penicillium (25%), Alternaria (24%), Cladosporium (20%), and Rhizopus (12%) and only in minor percentage by Aspergillus (3%) among others. The main fungal species isolated from genera Penicillium and Aspergillus were Penicillium expansum (57% RD) and A. section Nigri (97% RD). Of 17 analyzed Penicillium strains, 65% were able to produce at least one of the six mycotoxins analyzed in in vitro conditions by means of thin-layer chromatography method: citrinin, griseofulvin, patulin, cyclopiazonic acid, penitrem A, and roquefortin C.

scholarly journals DETERMINATION OF 10-GINGEROL IN INDIAN GINGER BY VALIDATED HPTLC METHOD OF SAMPLES COLLECTED ACROSS SUBCONTINENT OF INDIA

2016 ◽  
Vol 8 (12) ◽  
pp. 190
Author(s):  
Kamran Ashraf ◽  
Syed Adnan Ali Shah ◽  
Mohd Mujeeb
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

<p><strong>Objective: </strong>A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as perICH guidelines.</p><p><strong>Methods: </strong>The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F<sub>254</sub> using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm.</p><p><strong>Results: </strong>This system was found to have a compact spot of 10-gingerol at <em>R</em><sub>F</sub> value of 0.57±0.03. For the proposed procedure, linearity (<em>r</em><sup>2</sup> = 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory.</p><p><strong>Conclusion: </strong>Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India.</p>

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