Extraction, Purification of Tyrosinase and Tyrosinase Inhibitory Activity of Four Medicinal Plants from Nanded District (MS), India

2021 ◽  
Vol 1 (4) ◽  
pp. 320-324
Author(s):  
Mahesh P Jadhav ◽  
Swati G Swami
Keyword(s):  
Medicinal Plants ◽  
Inhibitory Activity ◽  
Spectroscopic Method ◽  
Precipitation Method ◽  
Phytochemical Screening ◽  
Annona Squamosa ◽  
Tyrosinase Inhibitory Activity ◽  
Ammonium Sulphate Precipitation ◽  
Precipitation Procedure ◽  
China Rose

Tyrosinase has an important role in melanin formation, is responsible for the production of colour pigments of skin, hair, and eye. In the presents study, tyrosinase was isolated from Mushrooms, isolation of enzyme was done by acetone precipitation procedure and precipitation of enzyme was done with ammonium sulphate precipitation method. Plants selected for extraction were Azadirachta indica (Neem), Manikara zapota (Chiku), Annona squamosa (Sitaphal), and Hibiscus Rosa-sinesis (China rose). For phytochemical screening Alkaloids-Mayer’s Test, Flavonoids (Shinoda Test, Alkaline Reagent Test), sugar (Benedict’s reagent Test), Glycosides (Borntrager's Test), Phenolic compounds Test (Ferric chloride Test, Gelatin Test, Lead Acetate Test). Mushroom tyrosinase inhibitory assay was determined by the spectroscopic method. The study shows the tyrosinase inhibitory activity of selected medicinal plants.

scholarly journals STUDY OF TYROSINASE INHIBITORY ACTIVITY AND PHYTOCHEMICAL SCREENING OF CASSIA FISTULA L. LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 384
Author(s):  
Caroline Wijaya ◽  
Berna Elya ◽  
Arry Yanuar
Keyword(s):  
Inhibitory Activity ◽  
Leaf Extract ◽  
Test Results ◽  
Phytochemical Screening ◽  
Cassia Fistula ◽  
Kinetic Assay ◽  
Tyrosinase Inhibitory Activity ◽  
Water Fraction ◽  
Ic50 Value ◽  
Phytochemical Constituents

Objective: This study was carried out to evaluate the phytochemical constituents and tyrosinase inhibitory activity of Cassia fistula leaves.Methods: A tyrosinase inhibitory activity assay was performed by measuring the decrease in the intensity of color suggestive of the inhibition ofdopachrome formation resulting from the L-DOPA-tyrosinase reaction.Results: The test results showed that the tyrosinase inhibitory activity of the water fraction of C. fistula leaf extract had the highest IC50 value(152.031 μg/mL) among other fractions (n-hexane, ethyl acetate, and n-butanol). An enzyme kinetic assay showed that the water fraction of C. fistulaleaf extract inhibited tyrosinase with mixed-type inhibition. Phytochemical screening showed that the water fraction of C. fistula leaf extract containedalkaloids, flavonoids, glycosides, phenols, and tannins.Conclusion: The current study indicated that C. fistula leaves possess significant tyrosinase inhibitory activity.

Tyrosinase inhibitory activity of selected medicinal plants

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
F Namjooyan ◽  
A Jahangiri ◽  
E Arkian ◽  
M Azemi
Keyword(s):  
Medicinal Plants ◽  
Inhibitory Activity ◽  
Tyrosinase Inhibitory Activity

scholarly journals Phytochemical screening and arginase inhibitory activity of extracts from several Tunisian medicinal plants

2019 ◽  
Vol 120 ◽  
pp. 313-318 ◽  
Author(s):  
R. Attia ◽  
C. Messaoud ◽  
K. Arraki ◽  
A. Zedet ◽  
C. Demougeot ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Inhibitory Activity ◽  
Phytochemical Screening

Tyrosinase Inhibitory Activity of Bangladeshi Indigenous Medicinal Plants

2000 ◽  
Vol 64 (9) ◽  
pp. 1967-1969 ◽  
Author(s):  
Firoza KHANOM ◽  
Hiroshi KAYAHARA ◽  
Koji TADASA
Keyword(s):  
Medicinal Plants ◽  
Inhibitory Activity ◽  
Tyrosinase Inhibitory Activity

scholarly journals Antidiabetic Activity of Extract Combination of Orthosiphon aristatus and Oryza sativa L. var glutinosa

2021 ◽  
Vol 4 (3) ◽  
pp. 202-209
Author(s):  
Eris Septiana ◽  
Nurul Maulida Rizka ◽  
Yadi Yadi ◽  
Partomuan Simanjuntak
Keyword(s):  
Biological Activity ◽  
Oryza Sativa ◽  
Medicinal Plants ◽  
Inhibitory Activity ◽  
Oryza Sativa L ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Screening ◽  
Ic50 Value

Traditionally and scientifically, research has shown that Orthosiphon aristatus and Oryza sativa L. var. glutinosa have antidiabetic activity. The combination of two medicinal plants can increase their biological activity. This study aimed to determine the antidiabetic activity of O. aristatus and O. sativa L. var. glutinosa on single and combined extracts. Phytochemical screening of the single extract was done qualitatively. The α-glucosidase inhibitory method was used as an antidiabetic activity. The results showed that every extract contained alkaloids, steroids/triterpenoids, flavonoids, tannins, quinones, and coumarins. A single extract of O. sativa L. var glutinosa, O. aristatus, and their combinations (1:1, 1:2, and 2:1) had an α-glucosidase enzyme inhibitory activity with an IC50 value of 67.82, 80.93, 73.81, 88.72, and 61.51 µg/ml, respectively. The combination shows that the ratio of 1:1 was nearly additive, 1:2 was slight to moderate antagonism, and 2:1 was moderate to slight synergism. The combination of 96% ethanol extract of O. sativa L. var. glutinosa and O. aristatus in a ratio of 2:1 was the most effective in increasing its inhibitory activity.

Review of Medicinal Plants and their Compounds for Aldose Reductase Inhibitory Activity

2018 ◽  
Vol 15 (7) ◽  
pp. 796-812
Author(s):  
Saliha Bouknana ◽  
Mohamed Bouhrim ◽  
Hayat Ouassou ◽  
Mohamed Bnouham
Keyword(s):  
Medicinal Plants ◽  
Aldose Reductase ◽  
Inhibitory Activity

Screening of Actinomycetes For α-amylase Inhibitors Production

2019 ◽  
Vol 15 (1) ◽  
pp. 41-45
Author(s):  
Shivabai Chandwad ◽  
Sudhakar Gutte
Keyword(s):  
Inhibitory Activity ◽  
Enzyme Inhibitors ◽  
Spectroscopic Method ◽  
Solid Material ◽  
Soil Samples ◽  
Assay Method ◽  
Novel Drugs ◽  
Pre Treatment ◽  
Diabetic Treatment ◽  
Amylase Inhibitors

Background:Diabetes mellitus is the most common and fastest growing disease in the world. One of the therapies to treat diabetes is the inhibition of α-amylase activity by inhibitors from microbial and plant source. Actinomycetes are potential sources of enzyme inhibitors, drugs, amino acids, vitamins etc.Objective:Our work mainly highlights the isolation of actinomycetes from soil samples of different habitats and screening of α -amylase inhibitors.Methods:Actinomycetes were isolated from soil samples of different habitats by different methods; these include a variety of pre-treatment of soil samples in combination with an appropriate supplement medium with selective antibacterial agents. Isolated actinomycetes grown in fermentation condition and metabolites were extracted with Isopropyl alcohol and concentrated to obtain solid material. The extract of each isolate was tested for α -amylase inhibition using starch Iodine plate method and DNS- spectroscopic method.Results:Total 110 actinomycetes strains were isolated from various sources. Among 110 extracts of actinomycetes, eight extracts have shown positive results for α-amylase inhibition in starch Iodine plate assay method. Extracts selected from primary results were used for the confirmation of inhibitory activity using DNS- spectroscopic method. Out of eight extracts, six extracts showed Porcine pancreatic α -amylase inhibitory activity ranging from 40-86%. The actinomycetes strains that produce α -amylase inhibitory activity are A-24, A-29, B-5, B-18, C-15 and D-24.Conclusion:These results show that actinomycetes are a potential source for α -amylase inhibitors, which may lead to valuable novel drugs for diabetic treatment.

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