scholarly journals First detection of Salmonella spp. in backyard production farms from central Chile

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Raul Alegria-Moran ◽  
Andres Lazo ◽  
Dacil Rivera ◽  
Viviana Toledo ◽  
Andrea Moreno-Switt ◽  
...  
Keyword(s):  
Public Health ◽  
Random Sampling ◽  
Production Systems ◽  
Regional Level ◽  
Salmonella Spp ◽  
Central Chile ◽  
Peptone Water ◽  
Random Sampling Approach ◽  
Sampling Approach

ObjectiveThe purpose of this study was to detect the presence of circulatingSalmonellaspp. on backyard production systems (BPS) with poultryor swine breeding in central ChileIntroductionCharacteristics and conditions of backyard production systems(BPS) transform them into potential maintainers of priority zoonoticagents, likeSalmonellaspp., highly important agent because of itsimpact in animal and public health (1).MethodsA stratified and proportional random sampling approach wasperformed (2), based on 15 provinces from the study area (regions ofValparaiso, Metropolitana and LGB O’Higgins). 329 BPS sampled(equivalent to 1,744 samples). Stool content inoculated in test tubeswith peptone water (APT, Difco®) supplemented with Novobiocin(Sigma®), incubated for 18 to 24 hours at 37° C. Subcultured onmodify semisolid Rappaport Vassiliadis (MSRV, Oxoid®) agarsupplemented with Novobiocin, incubated for 24 to 48 hours at 41.5° C.Samples compatible with growth and/or diffusion were sub-culturedby exhaustion on Xylose Lysine Deoxychocolate (XLD, Difco®) agarand then incubated for 24 hours at 37° C (3). Confirmation made byconventional PCR forinvAgenes (4). Serotypes were predicted usinga combination of PCR and sequencing, aimed directly at genes codingfor O, H1 and H2 antigens (5).Results1,744 samples were collected belonging to the 329 BPS. 15 positiveBPS (4.6%) detected. Serotypes detected correspond toSalmonellaTyphimurium (21.7%), followed bySalmonellaEnteritidis (13.0%)andSalmonellaInfantis (13.0%),SalmonellaHadar or Istanbul(8.7%),Salmonella[z42] or Tenessee (4.4%),SalmonellaKentucky(4.4) and unknown (34.8%) (Table 1).ConclusionsThis is the first evidence of serotypes ofSalmonellaspp. circulatingat a regional level in BPS from central Chile. A relevant pathogen forpublic health.

scholarly journals Nursing Practices in Dementia Management and Care in Nepal

2019 ◽  
Vol 3 (3) ◽  
pp. 83-90
Author(s):  
Krishna Prasad Pathak
Keyword(s):  
Registered Nurses ◽  
Random Sampling ◽  
Early Stage ◽  
Advice Giving ◽  
Dementia Patients ◽  
Problems And Solutions ◽  
Care Guidelines ◽  
Random Sampling Approach ◽  
Sampling Approach

Nurses fell barriers in management and care of dementia patients. A purposive random sampling approach identified 44 Registered Nurses (RN). The Knowledge of RNs’ with regard to the diagnosis, management and care of dementia was unsatisfactory on the aspects of  practices and management barriers are presented with regard to the following issues: negative views of dementia, difficulty in diagnosing early stage dementia, acceptability of specialists and responsibility for extra issues, knowledge of dementias and ageing, less awareness of declining abilities and diminished resources to handle care,  guidelines,  awareness of epidemiology and advice giving, and communicating the diagnosis.The following paper outlines the problems and solutions that Nepalese nurses’ in the community need to adopt in order to deal effectively with its diagnosis, care and management.

scholarly journals Pendugaan Stok Ikan Kuniran (Upeneus sulphureus, Cuvier 1829) di Perairan Selat Sunda, Banten

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Hikmatul Azizah ◽  
Mennofatria Boer ◽  
Nurlisa A. Butet
Keyword(s):  
Growth Pattern ◽  
Random Sampling ◽  
Demersal Fish ◽  
Fishing Gear ◽  
Stratified Random Sampling ◽  
Gender Ratio ◽  
Stock Status ◽  
Fishing Gears ◽  
Random Sampling Approach ◽  
Sampling Approach

Sulphur goatfish (Upeneus sulphureus) is one of a demersal fish as the main commodity in Sunda Strait. This research aimed to assess the stocks of sulfur goatfish (Upeneus sulphureus) in Sunda Strait, Banten as a basic management for optimum and sustainable used. Collecting samples were conducted with Stratified Random Sampling approach in May-October 2018 at PPP Labuan, Banten. The research showed the gender ratio of sulfur goatfish was 0.92:1. The growth pattern of the males fish was negative allometric and the female was isometric. Pukat cincin was the fishing gear become the standard for other fishing gears. Stock status of sulfur goatfish (Upeneus sulphureus) fisheries in Sunda Strait, Banten had indicated overfishing.  

scholarly journals PENYAKIT ANTRAKNOSA CABAI OLEH Colletotrichum sp. DI LAHAN RAWA KALIMANTAN SELATAN

2021 ◽  
Vol 23 (1) ◽  
pp. 30-36
Author(s):  
Mariana Mariana ◽  
Elly Liestiany ◽  
Fahmi Rizali Cholis ◽  
Nazwan Syahbani Hasbi
Keyword(s):  
Random Sampling ◽  
Yield Loss ◽  
Disease Incidence ◽  
Integrated Control ◽  
Disease Identification ◽  
High Incidence ◽  
Economic Constraints ◽  
Random Sampling Approach ◽  
Sampling Approach

[CHILLI ANTHRACNOSE DISEASE BY Colletotrichum sp. AT SWAMP LAND OF SOUTH KALIMANTAN PROVINCE].  Anthracnose is one of the major economic constraints for chili production worldwide. This disease is caused by the fungus Colletotrichum sp. The yield loss reaches 50% - 100% in the rainy season. In swamps, the incidence of this disease begins to increase. As the basis for the integrated control of anthracnose, it is necessary to record disease incidence and identify pathogens that attack conditions in swampsThe disease intensity survey was conducted in Hiyung chilli cultivation of Hiyung village lowland and the tidal swamplands of Marabahan district. The method is using Purposive Random Sampling approach. The Koch’s Postulates is used to determined the cause of the disease. Identification of pathogens that cause anthracnose is carried out in laboratories and greenhouses. Determination of obtained pathogen’s type is using literature references. The results of this study indicate that Hiyung chilli in the Hiyung village lowland shows a fairly high incidence of disease – i.e 43.78%, moreover, the result of Marabahan district tidal swamplands is 29.29%. Three Colletotrichum species were found, namely Colletotricum truncatum, C. gleosporiedes and C. acutatum.

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