scholarly journals Investigation of surface topography of different root-end filling materials: An in vitro study

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Mine Koruyucu ◽  
Hazal Özcan ◽  
Merve Bayram ◽  
Abdullah Burak Cankaya ◽  
Nurullah Keklikoglu ◽  
...  
Keyword(s):  
Surface Topography ◽  
Normal Saline ◽  
Phosphate Buffer ◽  
Saline Solution ◽  
In Vitro Study ◽  
Phosphate Buffer Saline ◽  
Filling Materials ◽  
Retrograde Filling ◽  
Group B

Aim: Although there are many materials that can be used for retrograde filling in surgical endodontics, none of them can be regarded as an ideal material yet. The purpose of this study was to compare the surface topography of three different root-end filling materials.Methods: 36 extracted single rooted human incisor teeth were cleaned and decoronated to standardized 10 mm root lengths. The root segments were prepared and 2 mm apical resection were performed. The samples were randomly separeted to three groups (Group A: Ca(OH)2, Group B: MTA Angelus, Group C: ProRoot MTA), each comprised of 12 roots. Materials were placed as 2 mm apical barriers and obturated with guttapercha and AH-Plus sealer. Each group dimidiated two subgroups (A1,A2,B1,B2,C1,C2). Groups A1,B1,C1 were stored in normal saline (NS), groups A2,B2,C2 were stored in neutral phosphate buffer saline (NPBS) solution and samples were incubated at 370C for 2 weeks. Stereomicroscope (32X) was used to photograph the root-end filling.Results: All specimens demonstrated white crystals formation and sediment over the root-end filling materials and on the superficial border of the root-end cavities’ wall as a white plague. A2,B2,C2 samples have more crystal sediment on root-end fillings than samples A1,B1,C1. Dissolution and corrosion were observed in groups A1, A2.Conclusions: The results of this study revealed that calcium hydroxide is more resorbable than MTA Angelus and ProRoot MTA. The crystals formation and precipitation were observed in neutral phosphate buffer saline solution was more than normal saline solution for all groups as a hydroxiapatite crystals.  

scholarly journals Coagulation effects of mannitol in combination with 0.9% normal saline or hydroxyethyl starch in patients undergoing supratentorial craniotomy: A preliminary report

2014 ◽  
Vol 01 (01) ◽  
pp. 056-059 ◽  
Author(s):  
Hemanshu Prabhakar ◽  
Gyaninder Singh ◽  
Parmod Bithal ◽  
Mani Kalaivani
Keyword(s):  
General Anaesthesia ◽  
Hydroxyethyl Starch ◽  
Normal Saline ◽  
In Vitro Study ◽  
Coagulation Parameters ◽  
Neurosurgical Patients ◽  
Group A ◽  
Clinically Significant ◽  
Group B

Abstract Background: Neurosurgical patients often require administration of both, mannitol and hydroxyethyl starch (HES). A recent in vitro study demonstrated that HES in combination with mannitol could disturb coagulation parameters and should be avoided in neurosurgical practice. The aim of our study was to evaluate coagulation abnormalities due to mannitol when administered alone and in combination with HES in patients undergoing craniotomy for various intracranial brain tumours. Materials and Methods: We enrolled 30 adult patients undergoing craniotomy. Patients were randomised into two groups using a computer generated randomisation chart. Interventions: Group A: Patients received 10 ml/kg 0.9% normal saline and 1 g/kg mannitol and Group B: Patients received 10 ml/kg, HES 130/0.4, and 1 gm/kg mannitol; immediately after induction of general anaesthesia. Rotational thromboelastography was done immediately after induction of general anaesthesia and 5 min after administration of mannitol. Measured parameters of blood coagulation were clotting time (CT) and clot formation time (CFT) with EXTEM and maximum clot firmness (MCF) with EXTEM and (FIBTEM). Results: Fourteen patients in each group completed the study. Insignificant change was noted in CT; CFT altered significantly from the baseline in both the groups (P < 0.05). MCF with FIBTEM did not change significantly from baseline (P > 0.05), but significantly differed between groups (P = 0.001). However, all values were in normal range. Conclusion: Mannitol 1 g/kg and HES 10 ml/kg can be safely administered in patients undergoing craniotomy for supratentorial tumours, without clinically significant changes in coagulation parameters.

scholarly journals Comparative evaluation of microleakage of various retrograde filling materials: An in vitro study

2013 ◽  
Vol 4 (2) ◽  
pp. 403 ◽  
Author(s):  
Virat Galhotra ◽  
Anjali Sofat ◽  
InderK Pandit ◽  
RamandeepSingh Gambhir ◽  
Nikhil Srivastava ◽  
...  
Keyword(s):  
Comparative Evaluation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Filling Materials ◽  
Retrograde Filling

scholarly journals Comparison of Root End Sealing Ability of Three Retrograde Filling Materials in Teeth with Root Apices Resected at 90° using Dye Penetration Method under Fluorescent Microscope: An In-vitro Study

2021 ◽  
pp. 319-325
Author(s):  
Payal Chaudhari ◽  
Manoj Chandak ◽  
Arvind Ramdas ◽  
Patil Bhagat
Keyword(s):  
Bone Cement ◽  
In Vitro Study ◽  
Filling Material ◽  
Dye Penetration ◽  
Fluorescent Microscope ◽  
Filling Materials ◽  
Anterior Teeth ◽  
Sealing Ability ◽  
Retrograde Filling

Background: Inadequate seal at the apex is the substantial cause for surgical endodontic dissatisfaction. The retrograde filling material which is used should prevent the egress of potential contaminants into periradicular tissue. Objectives: To evaluate the ability of MTA Angelus, Zirconomer and Bioactive bone cement to seal the root end as retrograde filling material; and to compare root end sealing ability of these three different retrograde filling materials with apices resected at 900 angles using dye penetration method under fluorescent microscope. Methodology: Thirty six extracted upper anterior teeth are to be cut horizontally at the CEJ. After following the standard protocols of, “cleaning, shaping and obturation” with gutta percha and “AH Plus sealer”, the samples will be resected 3mm at the apical end at 90 degree angle along the long axis of the tooth with the help of diamond disc. A root end cavity of depth 3mm will be made with Diamond coated ultrasonic surgical tip S12 90 ND. The teeth will be randomly categorized in 3 groups: Group 1: MTA angelus; Group 2: Zirconomer; Group 3: Bioactive Bone Cement. Following which the roots to be coated with nail varnish except the tip. Each material will be compressed in the root end cavity with the help of small pluggers. All the samples which are retrofilled will be kept in acrydine orange for a duration of 24 hours, following which cleaning & bucco-lingual sectioning(vertical) will be done. Fluorescent microscope will be used for observation of sectioned root samples. Expected Results: Bioactive bone cement is expected to have better sealing ability of the retrograde cavity preparation with minimal or no microleakage followed by MTA Angelus and then Zirconomer. Conclusion: If this study proves correct, this would be helpful for the clinicians to choose better and the most efficient retrograde filling material with best sealing ability and minimal microleakage in a retrograde preparation which will aid in success of the root canal treatment further resolving the infection.

scholarly journals Degradation behavior of theophylline/chitosan/?-cyclodextrin microspheres for pulmonary drug delivery

2016 ◽  
Vol 11 ◽  
pp. S116-S122 ◽  
Author(s):  
Li Wang ◽  
Tingting Yang ◽  
Weiping Ju ◽  
Ming Ren ◽  
Zhanqin Feng ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Phosphate Buffer ◽  
In Vitro Study ◽  
Pulmonary Drug Delivery ◽  
Degradation Behavior ◽  
Sprague Dawley ◽  
Phosphate Buffer Saline ◽  
Drug Delivery Carrier

To evaluate the degradation behavior of theophylline/chitosan/?-cyclo-dextrin microspheres, we performed both in vitro study by putting the microspheres in phosphate buffered saline or in phosphate buffer saline with enzyme and in vivo study by implanting the microspheres into the back of male Sprague-Dawley rats. The results showed that microspheres were degraded in enzymatic hydrolysis and phosphate buffer saline, which were degraded faster in 0.2 mg/mL lysozyme than in phosphate buffer saline. The morphology of microspheres in phosphate buffer saline and enzyme solution developed rough surfaces, and showed irregular shape and pores after 8 weeks. The microspheres were degraded in vivo within 8 weeks with irregular, sheet, porous morphology, and the diameters were smaller than 5 ?m. These results indicated that the theophylline/chitosan/?-cyclodextrin microspheres had a good degradation both in vitro and in vivo which can be used as a pulmonary drug delivery carrier. 

scholarly journals Sealing Efficiency of MTA, Accelerated MTA, Biodentine and RMGIC as Retrograde Filling Materials

2021 ◽  
Vol 25 (3) ◽  
pp. 159-165
Author(s):  
Merve Mutluay ◽  
Abidin Talha Mutluay
Keyword(s):  
Paired Comparison ◽  
In Vitro Study ◽  
Blue Dye ◽  
Dye Penetration ◽  
Filling Materials ◽  
Nail Polish ◽  
Retrograde Filling ◽  
Significant Difference ◽  
Group 3

Summary Background/Aim: This in-vitro study evaluated the apical-sealing ability of MTA, MTA+10% CaCl2, Biodentine™ and RMGIC when used as retrograde material. Material and Methods: A total of 80 single-rooted bovine incisors were decoronated, instrumented, and divided into 4 groups according to retrograde material, as follows: Group 1: MTA (ProRoot MTA, Dentsply); Group 2: MTA (ProRoot MTA, Dentsply) + 10% CaCl2; Group 3: Biodentine (Biodentine®, Septodont); Group 4: RMGIC (Nova Glass -LC, Imicryl). Root surfaces were isolated with nail polish, and teeth were immersed in 2% methylene blue dye at 37°C for 48 h. The extent of dye penetration (mm) was measured under a stereomicroscope. Results: RMGIC had the highest mean-rank dye penetration score (MP=49.05), followed by MTA (MP=43.65), Biodentine (35.95) and MTA+CaCl2 (MP=33.35). The results of paired comparison tests found the mean microleakage value of MTA+10% CaCl2 and Biodentine (Group 3) to be significantly lower than that of RMGIC (respectively; p=0,020, p=0,019). No significant difference was found in the other group comparisons (p> 0.05). Conclusions: These results suggest that the addition of an accelerator to MTA may reduce microleakage following endodontic surgery. Biodentine can be used as an alternative to MTA on retrograde obturation.

In vitro study of the sealing ability of four retrograde filling materials

1988 ◽  
Vol 4 (2) ◽  
pp. 82-84 ◽  
Author(s):  
Panajiotis Beltes ◽  
Panos Zervas ◽  
Theodor Lambrianidis ◽  
Loannis Molyvdas
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Filling Materials ◽  
Sealing Ability ◽  
Retrograde Filling
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