Evaluation of Anthelmintic properties of Sesbania grandiflora Pers. (Kathurumurunga) against larvae of Toxocara canis and Haemonchus contortus – In Vitro Study

The Healer ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 42-47
Author(s):  
ERHSS Ediriweera
Keyword(s):  
Sri Lanka ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
In Vitro Study ◽  
Toxocara Canis ◽  
Positive Control ◽  
Migration Inhibition ◽  
Larval Migration ◽  
Sesbania Grandiflora

Sesbania grandiflora is a slender tree. It is a common garden plant in Sri Lanka, which grows well in mid and low country. In Sri Lanka, Sesbania grandiflora is used as a home remedy, in treatment of worm infections in humans. Toxocara canis is a helminth parasite infecting dogs and other canids. It also causes toxocariasis in humans. Haemonchus contortus is a nematode that infects goats and causes anaemia, marked reduction in growth and reproduction, and even death. Using this information, in vitro larvae migratory inhibition assay was carried out on Toxocara canis and Haemonchus contortus larve. This study revealed 98.1% and 94.3% larvae migration inhibition with larvae of Toxocara canis and Haemonchus contortus respectively. Least number of migrated larvae was observed in the positive control Levamisole and all the larvae were dead after migration. In decoction of S.grandiflora, all the migrated Toxocara larvae were dead and Haemonchus larvae were dead or in Grade1 (inactive but occasional movement can be observed) condition. Inhibition of Toxocara larval migration and Haemonchus larval migration with decoction of Sesbania grandiflora and Levamisole are statically significant (p < 0.05). Since mean of LMI (larval migration inhibition) of Levamisole is greater than mean of LMI of Sesbania grandiflora with both larvae, Levamisole is more effective than Sesbania grandiflora. Based on these findings, the aqueous extract of leaves of Sesbania grandiflora is shows a statistically significant anthelmintic activity in in-vitro model.

scholarly journals The effects of flubendazole and its metabolites on the larval development of Haemonchus contortus (Nematoda: Trichostrongylidae): an in vitro study

2010 ◽  
Vol 47 (4) ◽  
pp. 269-272 ◽  
Author(s):  
H. Bártíková ◽  
L. Skálová ◽  
J. Lamka ◽  
B. Szotáková ◽  
M. Várady
Keyword(s):  
Larval Development ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
In Vitro Study ◽  
Lethal Concentration ◽  
Resistant Isolate ◽  
Susceptible Isolate ◽  
Marked Resistance ◽  
Larval Development Test

AbstractThe anthelmintic effects of flubendazole (FLU), its two main metabolites reduced flubendazole (FLU-R) and hydrolyzed flubendazole (FLU-H), and thiabendazole (TBZ) were compared using an in vitro larval development test in two isolates of Haemonchus contortus, a fully susceptible isolate (HCS) and a multi-resistant isolate (HCR). Results were quantified as 50 % lethal concentration (LC50), 99 % lethal concentration (LC99), efficacy factor (EF), and resistance factor (RF). For HCS, both LC50 and LC99 of FLU were lower than those of the reference TBZ. The anthelmintic activity of FLU-R in HCS and HCR was 13 and 6 times lower than the activity of FLU, respectively. The anthelmintic activity of FLU-H was negligible (approximately 363–853 times lower) compared to that of FLU. Although a marked resistance of the HCR isolate to TBZ was confirmed, only a low tolerance to FLU-R and slightly higher tolerance to FLU were found.

scholarly journals In vitro anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark waste against Haemonchus contortus obtained from a local slaughterhouse in Indonesia

2020 ◽  
Vol 13 (8) ◽  
pp. 1549-1554
Author(s):  
Zein Ahmad Baihaqi ◽  
Irkham Widiyono ◽  
Wisnu Nurcahyo
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Positive Control ◽  
Sem Analysis ◽  
The Body ◽  
Paraserianthes Falcataria ◽  
Petri Dishes ◽  
Ethanol Extracts

Aim: This study was conducted to determine the anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark against Haemonchus contortus. Materials and Methods: Ethanol extract of bark (E.E.B.) waste and aqueous extract of bark (A.E.B.) waste of P. falcataria (at concentrations 0, 0.2, 0.4, 0.8, 1, 2.5, and 5%) and albendazole (2 mg/ml) as the positive control were placed in separate Petri dishes (50 mm). Twenty H. contortus worms were placed in Petri dishes and incubated at 37°C for 0.5, 1, 2, 3, 4, 5, 6, and 12 h. Mortality of each worm was ensured by pressing the body of the worm with a pair of tweezers and keeping it in lukewarm water for 5 min before declaring it dead. Mortality is defined as amount of death individuals and time of mortality of each worm was recorded. The parasites were then observed using scanning electron microscopy (SEM) at an accelerating voltage of 15 Kv. Statistical analysis was performed using SPSS 21.0 software, two-way ANOVA followed by Tukey's test to detect significant differences (p<0.05). The result was expressed as the mean ± standard deviation. Results: The E.E.B. and A.E.B. of P. falcataria contained active compounds, such as tannin, alkaloid, flavonoid, saponin, steroid, and triterpenoid. E.E.B. had a higher content of phenol, while A.E.B. had a higher content of flavonoid. In this study, P. falcataria showed a significant effect (p=0.00) on H. contortus in vitro. E.E.B. (0.8%) was able to exterminate H. contortus completely after 6 h, more effective than A.E.B. (5%) while the positive control requires (2 mg/ml) after 2 h. SEM analysis of the worm treated with E.E.B. and A.E.B. showed damaged cuticle structure. Conclusion: The aqueous and ethanol extracts of P. falcataria bark waste demonstrated anthelmintic activity against H. contortus.

In vitro larvicidal and in vivo anthelmintic effects of Oxalis tetraphylla (Oxalidaceae) hydroalcoholic extract against Haemonchus contortus in lambs

2017 ◽  
Vol 92 (3) ◽  
pp. 309-316 ◽  
Author(s):  
B.J. González-Cruz ◽  
M. Rodríguez-Labastida ◽  
M. González-Cortázar ◽  
A. Zamilpa ◽  
M.E. López-Arellano ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Larval Mortality ◽  
Anthelmintic Activity ◽  
Positive Control ◽  
Plate Method ◽  
Hydroalcoholic Extract ◽  
Stage 1 ◽  
Two Stages

AbstractThe in vitro larvicidal and in vivo anthelmintic effects of Oxalis tetraphylla hydroalcoholic extract (HE), against Haemonchus contortus in experimentally infected lambs, were assessed. We used a microtitration plate method, comprising the following two stages. Stage 1: 20 μl of water containing 200 sheathed H. contortus infective larvae (ShHcl) were deposited in every well of three series; then, the series 2 and 3 wells were treated with 80 μl 1% ivermectin and O. tetraphylla HE at 20 mg/ml, respectively. Stage 2: the same procedure was performed replacing the ShHcl with exsheathed larvae (ExShHcl). Evaluations were performed after 24 and 48 h. The total numbers of dead and live larvae were counted. A second experiment evaluated the reduction in nematode egg populations in the faeces of lambs treated orally with the O. tetraphylla HE. The 27 lambs used were divided into Groups 1, 2 and 3 (n = 9), which were administered water (positive control), levamisole 1 m (7.5 mg/kg body weight (BW), as a unique dose) and O. tetraphylla HE (20 mg/kg BW), respectively. The plant HE was administered daily for 8 days. The in vitro assay showed 80.9% and 86.5% larval mortality of ShHcl after 24 and 48 h, respectively, while the corresponding mortality values for ExShHcl were 97 and 99%, respectively. The in vivo assay showed variability in the eggs/gram of faeces (epg) values; however, at the end of the trial, the average reduction in the epg values of the O. tetraphylla HE group was 45.6% (P < 0.05). Oxalis tetraphylla HE contains compounds that belong to the flavonol group with anthelmintic activity.

scholarly journals Effets De La Digestion Gastrique Sur Les Propriétés Anthelminthiques De Zanthoxylum Zanthoxyloides (Lam.) Zepernick & Timlerto Et De Newbouldia Laevis (P.Beauv.) Sur Haemonchus Contortus

2017 ◽  
Vol 13 (24) ◽  
pp. 204
Author(s):  
Irvine Yèinou Minaflinou Sacca Sidi ◽  
Géorcelin Goué Alowanou ◽  
Esaïe Tchétan ◽  
Maliki Youssouf Aminou ◽  
Sylvie Mawulé Hounzangbé-Adoté ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Gastrointestinal Nematodes ◽  
The Other ◽  
Migration Inhibition ◽  
Traditional Use ◽  
Larval Migration ◽  
Methanolic Extracts ◽  
Zanthoxylum Zanthoxyloides ◽  
In Sacco

Several recent studies have shown that medicinal plants Zanthoxylum zanthoxyloides (Fagara) and Newbouldia laevis possess anthelmintic activities in vitro on different stages of development of gastrointestinal nematodes. The objective of this study was to evaluate the in vitro anthelmintic properties of residues from digestion in the rumen of leaf powders of both plants on the migration of the 3rd-stage larvae L3s of Haemonchus contortus. Residues obtained after incubation at 0 h, 24 h and 96 h kinetic points of the leaf powders of both plants in the rumen of sheep with fistulae were used for the assay. The larval migration inhibition test evaluated the anthelmintic properties of the methanolic extracts of residues of the two plants. The effect of plant extracts on larval migration was notdose- dependent (p> 0.05) but was a function of plant incubation time (p 0.05). N. Laevis seems to have retained his anthelmintic property after incubation in sacco in the rumen. On the other hand, Fagara seems to lose its effectiveness as it stays in the rumen. Findings obtained on these plants confirm their traditional use in veterinary medicine especially in the control of helminthiasis.

Anthelmintic evaluation of Cucurbita pepo againstgastrointestinal nematodes of sheep

2017 ◽  
Author(s):  
Ambalathaduvar Meenakshisundaram ◽  
Tirunelveli Jayagopal Harikrishnan ◽  
Thavasi Anna
Keyword(s):  
Larval Development ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Migration Inhibition ◽  
Egg Hatch ◽  
Larval Migration ◽  
Faecal Egg Count ◽  
Ethanolic Extracts

The study was aimed at to investigate the anthelmintic efficacy of aqueous and ethanolic extracts of Cucurbita pepo against gastrointestinal nematodes of sheep by scientifically validated in vitro and in vivo tests. In vitro anthelmintic activity was determined by egg hatch assay, larval migration inhibition assay and larval development assay against eggs and larvae of mixed gastrointestinal nematodes of sheep. The extracts which were found to be effective in in vitro assays were further evaluated by in vivo faecal egg count reduction test against mixed species of sheep gastrointestinal nematodes with 3 different doses (125, 250 and 500 mg kg-1). Haematological and serum parameters were estimated before and after treatment to determine the impact of plant extracts on health of the animal. Aqueous extract of C. pepo demonstrated significant inhibition of egg hatch at 40 and 80 mg/ml. The ED50 value of egg hatch inhibition was 3.51mg/ml. In larval migration inhibition assay, both aqueous and ethanolic extracts of C. pepo demonstrated inhibition of larval migration and the LM50 was 1.75 and 0.32 mg/ml respectively. However, in larval development assay, both aqueous and ethanolic extracts showed only marginal inhibition of larval development at all concentrations tested. In faecal egg count reduction test (FECRT), aqueous extracts of C. pepo at 500 mg/kg caused significant reduction in eggs per gram (96.61 per cent) the reduction being higher than the result obtained with albendazole, the positive control (93.25 per cent). There was a significant difference in EPG reduction between sheep treated with albendazole and C. pepo. Haematological and serum parameters were not affected in both the treated and untreated sheep indicating that the extracts did not cause toxic effects. Based on the results obtained in this study, the aqueous extract of C. pepo possesses anthelmintic activity and could offer an alternative source for the control of gastrointestinal nematodes of sheep.

scholarly journals In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
X. De Jesús-Martínez ◽  
A. Olmedo-Juárez ◽  
J. Olivares-Pérez ◽  
A. Zamilpa ◽  
P. Mendoza de Gives ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Methanolic Extract ◽  
Anthelmintic Activity ◽  
Lethal Effect ◽  
Positive Control ◽  
Probit Analysis ◽  
Egg Hatching ◽  
Infective Larvae

The aim of this study was to evaluate the in vitro lethal effect of a methanolic extract (ME) from Caesalpinia coriaria fruits against Haemonchus contortus eggs and infective larvae. The anthelmintic activity was assessed using the egg hatching inhibition assay (EHI) and the mortality test. The ME was assessed using five concentrations as follows: 6.15, 3.12, 1.56, and 0.78 mg/mL to eggs and 150, 100, 75, and 50 mg/mL to larvae, respectively. Ivermectin (5 mg/mL) was used as positive control and 4% methanol and distilled water were used as negative controls. The data of ovicidal and larvicidal effect were analyzed with a completely randomized design through ANOVA analysis using the general linear model (GLM) and lethal concentrations (LC50 and LC90) were estimated through a Probit analysis using the SAS program. A clear ME increased concentration dependence effect was observed in the EHI and mortality tests. The highest activity of the methanolic extract was observed at the highest concentration (P < 0.05) to obtain a similar effect to the positive control (ivermectin), with LC50 = 78.38 and 0.00064 mg/mL and LC90 =235.63 and 0.024 mg/mL, respectively, for larvae and eggs. The results indicate that the C. coriaria fruit ME possesses in vitro ovicidal and larvicidal properties (gallotannins: methyl gallate) against H. contortus that needs to be investigated more in vivo for the control of gastroenteric nematodes in ruminants.

Effect of molecular weight and concentration of legume condensed tannins on in vitro larval migration inhibition of Haemonchus contortus

2014 ◽  
Vol 199 (1-2) ◽  
pp. 93-98 ◽  
Author(s):  
H.D. Naumann ◽  
S.A. Armstrong ◽  
B.D. Lambert ◽  
J.P. Muir ◽  
L.O. Tedeschi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Haemonchus Contortus ◽  
Condensed Tannins ◽  
Migration Inhibition ◽  
Larval Migration

Chemical characterization and in vitro anthelmintic activity of Citrus bergamia Risso and Citrus X paradisii Macfad essential oil against Haemonchus contortus Kirby isolate

Acta Tropica ◽  
2021 ◽  
Vol 217 ◽  
pp. 105869
Author(s):  
Vivien Patricia Garbin ◽  
Beatriz Munguía ◽  
Jenny Carolina Saldaña ◽  
Cícero Deschamps ◽  
Roger Raupp Cipriano ◽  
...  
Keyword(s):  
Essential Oil ◽  
Haemonchus Contortus ◽  
Chemical Characterization ◽  
Anthelmintic Activity ◽  
Citrus Bergamia

In vitro anthelmintic activity of Dennettia tripetala G. Baker (Annonaceae) Fruits against Haemonchus contortus

2021 ◽  
Author(s):  
Rachel A. Nwosu ◽  
Mohammed M. Suleiman ◽  
Hussaina J. Makun ◽  
Matthew P. Ameh ◽  
Manji A. Shetshak ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Dennettia Tripetala

In Vitro Nematocidal Effect and Anthelmintic Activity of Artemisia cina Against Haemonchus contortus in Gerbils and Relative Expression of Hc29 Gene in Transitional Larvae (L3–L4)

2021 ◽  
Author(s):  
Rosa Isabel Higuera-Piedrahita ◽  
Mariana Dolores-Hernández ◽  
Luis Gerardo Jiménez-Pérez ◽  
Brígida C. Camacho-Enríquez ◽  
Alejandro Zamilpa ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Relative Expression
Sign in / Sign up

Export Citation Format

Share Document