scholarly journals P1BS.8 - Enzyme Biosensor Based on Tobacco Mosaic Virus-Modified Field-Effect Structures

2018 ◽  
Author(s):  
M. Jablonski ◽  
A. Poghossian ◽  
D. Molinnus ◽  
M. J. Schöning ◽  
C. Koch ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Field Effect ◽  
Enzyme Biosensor

(IMCS Second Place Best Paper Award) High-Density Immobilization of Tobacco Mosaic Virus Nanotubes As Enzyme Nanocarriers Onto Field-Effect Biosensor Structures

2021 ◽  
Vol MA2021-01 (63) ◽  
pp. 1695-1695
Author(s):  
Melanie Jablonski ◽  
Robin Severins ◽  
Arshak Poghossian ◽  
Christina Wege ◽  
Michael Keusgen ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Field Effect ◽  
High Density ◽  
Paper Award

scholarly journals Microwave assisted synthesis and characterisation of a zinc oxide/tobacco mosaic virus hybrid material. An active hybrid semiconductor in a field-effect transistor device

2015 ◽  
Vol 6 ◽  
pp. 785-791 ◽  
Author(s):  
Shawn Sanctis ◽  
Rudolf C Hoffmann ◽  
Sabine Eiben ◽  
Jörg J Schneider
Keyword(s):  
Zinc Oxide ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Hybrid Material ◽  
Zno Nanoparticles ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Processing Technique ◽  
Microwave Assisted Synthesis ◽  
Effect Transistor

Tobacco mosaic virus (TMV) has been employed as a robust functional template for the fabrication of a TMV/zinc oxide field effect transistor (FET). A microwave based approach, under mild conditions was employed to synthesize stable zinc oxide (ZnO) nanoparticles, employing a molecular precursor. Insightful studies of the decomposition of the precursor were done using NMR spectroscopy and material characterization of the hybrid material derived from the decomposition was achieved using dynamic light scattering (DLS), transmission electron microscopy (TEM), grazing incidence X-ray diffractometry (GI-XRD) and atomic force microscopy (AFM). TEM and DLS data confirm the formation of crystalline ZnO nanoparticles tethered on top of the virus template. GI-XRD investigations exhibit an orientated nature of the deposited ZnO film along the c-axis. FET devices fabricated using the zinc oxide mineralized virus template material demonstrates an operational transistor performance which was achieved without any high-temperature post-processing steps. Moreover, a further improvement in FET performance was observed by adjusting an optimal layer thickness of the deposited ZnO on top of the TMV. Such a bio-inorganic nanocomposite semiconductor material accessible using a mild and straightforward microwave processing technique could open up new future avenues within the field of bio-electronics.

scholarly journals Towards Multi-Analyte Detection with Field-Effect Capacitors Modified with Tobacco Mosaic Virus Bioparticles as Enzyme Nanocarriers

Biosensors ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 43
Author(s):  
Melanie Welden ◽  
Arshak Poghossian ◽  
Farnoosh Vahidpour ◽  
Tim Wendlandt ◽  
Michael Keusgen ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Field Effect ◽  
Layer Structure ◽  
Logic Gate ◽  
High Surface ◽  
Volume Ratio ◽  
Surface Binding ◽  
Immobilization Of Enzymes ◽  
Analyte Detection

Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO2-Ta2O5 layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1–3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta2O5-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate.

scholarly journals Capacitive Field-Effect Biosensor Studying Adsorption of Tobacco Mosaic Virus Particles

Micromachines ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 57
Author(s):  
Melanie Jablonski ◽  
Arshak Poghossian ◽  
Robin Severins ◽  
Michael Keusgen ◽  
Christina Wege ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Field Effect ◽  
Label Free ◽  
Sensor Surface ◽  
Virus Particles ◽  
Biochemical Sensing ◽  
Label Free Detection ◽  
Sensitive Parameters ◽  
The Impact

Plant virus-like particles, and in particular, tobacco mosaic virus (TMV) particles, are increasingly being used in nano- and biotechnology as well as for biochemical sensing purposes as nanoscaffolds for the high-density immobilization of receptor molecules. The sensitive parameters of TMV-assisted biosensors depend, among others, on the density of adsorbed TMV particles on the sensor surface, which is affected by both the adsorption conditions and surface properties of the sensor. In this work, Ta2O5-gate field-effect capacitive sensors have been applied for the label-free electrical detection of TMV adsorption. The impact of the TMV concentration on both the sensor signal and the density of TMV particles adsorbed onto the Ta2O5-gate surface has been studied systematically by means of field-effect and scanning electron microscopy methods. In addition, the surface density of TMV particles loaded under different incubation times has been investigated. Finally, the field-effect sensor also demonstrates the label-free detection of penicillinase immobilization as model bioreceptor on TMV particles.

High-Density Immobilization of Tobacco Mosaic Virus Nanotubes As Enzyme Nanocarriers Onto Field-Effect Biosensor Structures

2020 ◽  
Vol MA2020-01 (35) ◽  
pp. 2484-2484
Author(s):  
Melanie Jablonski ◽  
Robin Severins ◽  
Arshak Poghossian ◽  
Christina Wege ◽  
Michael Keusgen ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Field Effect ◽  
High Density

scholarly journals Field-Effect Biosensors Modified with Tobacco Mosaic Virus Nanotubes as Enzyme Nanocarrier

Proceedings ◽  
2017 ◽  
Vol 1 (4) ◽  
pp. 505 ◽  
Author(s):  
Melanie Jablonski ◽  
Claudia Koch ◽  
Thomas S. Bronder ◽  
Arshak Poghossian ◽  
Christina Wege ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Field Effect

Quantitative Measurements on the Ion Etching of TMV

1973 ◽  
Vol 31 ◽  
pp. 336-337
Author(s):  
Irwin Bendet ◽  
Nabil Rizk
Keyword(s):  
Electron Microscope ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ion Current ◽  
Beam Diameter ◽  
Ion Etching ◽  
Preliminary Results ◽  
Quantitative Measurements ◽  
Monitoring Devices

Preliminary results reported last year on the ion etching of tobacco mosaic virus indicated that the diameter of the virus decreased more rapidly at 10KV than at 5KV, perhaps reaching a constant value before disappearing completely.In order to follow the effects of ion etching on TMV more quantitatively we have designed and built a second apparatus (Fig. 1), which incorporates monitoring devices for measuring ion current and vacuum as well as accelerating voltage. In addition, the beam diameter has been increased to approximately 1 cm., so that ten electron microscope grids can be exposed to the beam simultaneously.

Tobacco Mosaic Virus-Infected Tissue

1985 ◽  
Vol 43 ◽  
pp. 510-511
Author(s):  
Egbert W. Henry
Keyword(s):  
Nicotiana Tabacum ◽  
Tobacco Mosaic Virus ◽  
Chlorogenic Acid ◽  
Mosaic Virus ◽  
Host Resistance ◽  
Oxidative Metabolism ◽  
Leaf Tissue ◽  
Vein Clearing ◽  
Basal Septum ◽  
Concentration Levels

Tobacco mosaic virus (TMV) infection has been studied in several investigations of Nicotiana tabacum leaf tissue. Earlier studies have suggested that TMV infection does not have precise infective selectivity vs. specific types of tissues. Also, such tissue conditions as vein banding, vein clearing, liquification and suberization may result from causes other than direct TMV infection. At the present time, it is thought that the plasmodesmata, ectodesmata and perhaps the plasmodesmata of the basal septum may represent the actual or more precise sites of TMV infection.TMV infection has been implicated in elevated levels of oxidative metabolism; also, TMV infection may have a major role in host resistance vs. concentration levels of phenolic-type enzymes. Therefore, enzymes such as polyphenol oxidase, peroxidase and phenylalamine ammonia-lyase may show an increase in activity in response to TMV infection. It has been reported that TMV infection may cause a decrease in o-dihydric phenols (chlorogenic acid) in some tissues.

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