scholarly journals Cloning DNA Vaccine Candidate SAG1 Gene of Toxoplasma gondii

2016 ◽  
Vol 39 (4) ◽  
pp. 255-259
Author(s):  
Husniye Lalek ◽  
Esra Gurbuz ◽  
Serkan Karaca ◽  
Suleyman Yazar ◽  
Salih Kuk
Keyword(s):  
Toxoplasma Gondii ◽  
Dna Vaccine ◽  
Vaccine Candidate ◽  
Sag1 Gene

scholarly journals Immunogenicity and Protective Efficiency in Mice of a Smallpox DNA Vaccine Candidate

2017 ◽  
Vol 08 (01) ◽  
Author(s):  
Na Young Kim ◽  
Dong Suk Chang ◽  
Gyeung Haeng Hur ◽  
Taek Yeol Lee ◽  
Jai Myung Yang ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Vaccine Candidate ◽  
Protective Efficiency

scholarly journals In Silico Prediction of T and B Cell Epitopes of SAG1-Related Sequence 3 (SRS3) Gene for Developing Toxoplasma gondii Vaccine

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Abolfazl Mirzadeh ◽  
Geita Saadatnia ◽  
Majid Golkar ◽  
Jalal Babaie ◽  
Samira Amiri ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Dna Vaccine ◽  
In Silico ◽  
Effective Vaccine ◽  
T Cell Epitopes ◽  
In Silico Prediction ◽  
Vaccine Strategy ◽  
Related Sequence ◽  
Ideal Tool ◽  
Major Surface

: Toxoplasmosis is a worldwide infection that can lead to serious problems in immune-compromised individuals and fetuses. A DNA vaccine strategy would be an ideal tool against Toxoplasma gondii. One of the necessary measures to provide an effective vaccine is the selection of proteins with high antigenicity. The SAG1-related sequence 3 (SRS3) protein is a major surface antigen in T. gondii that can be used as a vaccine candidate. In the present study, bioinformatics and computational methods were utilized to predict protein characteristics, as well as secondary and tertiary structures. The in silico approach is highly suited to analyze, design, and evaluate DNA vaccine strategies. Hence, in silico prediction was used to identify B and T cell epitopes and compare the antigenicity of SRS3 and other candidate genes of Toxoplasma previously applied in the production of vaccines. The results of the analysis theoretically showed that SRS3 has multiple epitopes with high antigenicity, proposing that SRS3 is a promising immunogenic candidate for the development of DNA vaccines against toxoplasmosis.

scholarly journals One or two dose regimen of the SARS-CoV-2 synthetic DNA vaccine INO-4800 protects against respiratory tract disease burden in nonhuman primate challenge model

2021 ◽  
Author(s):  
Karen Gooch ◽  
Trevor Smith ◽  
Francisco Salguero ◽  
Susan Fotheringham ◽  
Robert Watson ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Dose Regimen ◽  
Neutralizing Antibodies ◽  
Vaccine Candidate ◽  
Histopathological Examination ◽  
Macaque Model ◽  
Synthetic Dna ◽  
Intradermal Delivery ◽  
Viral Dose ◽  
Tract Disease

Abstract Safe and effective vaccines will provide essential medical countermeasures to tackle the COVID-19 pandemic. Here, we evaluate the safety, immunogenicity and efficacy of the intradermal delivery of INO-4800, a synthetic DNA vaccine candidate encoding the SARS-CoV-2 spike protein in the rhesus macaque model. Single and 2 dose vaccination regimens were evaluated. Vaccination induces both binding and neutralizing antibodies, along with IFN-γ-producing T cells against SARS-CoV-2. Upon administration of a high viral dose (5 x 106 pfu) via the intranasal and intratracheal routes we observe significantly reduced virus load in the lung and throat, in the vaccinated animals compared to controls. 2 doses of INO-4800 is associated with more robust vaccine-induced immune responses and improved viral protection. Importantly, histopathological examination of lung tissue provides no indication of vaccine-enhanced disease following SARS-CoV-2 challenge in INO-4800 immunized animals. This vaccine candidate is currently under clinical evaluation as a 2 dose regimen.

scholarly journals Molecular docking of immunogenic peptide of Toxoplasma gondii and encapsulation with polymer as vaccine candidate

2018 ◽  
Vol 46 (sup2) ◽  
pp. 744-754 ◽  
Author(s):  
Rabia Cakir-Koc ◽  
Yasemin Budama-Kilinc ◽  
Yagmur Kokcu ◽  
Serda Kecel-Gunduz
Keyword(s):  
Molecular Docking ◽  
Toxoplasma Gondii ◽  
Vaccine Candidate ◽  
Immunogenic Peptide

scholarly journals Immunogenicity and Protective Effect of a Virus-Like Particle Containing the SAG1 Antigen of Toxoplasma gondii as a Potential Vaccine Candidate for Toxoplasmosis

Biomedicines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 91
Author(s):  
Won Hyung Choi ◽  
Ji Sun Park
Keyword(s):  
Toxoplasma Gondii ◽  
Vaccine Candidate ◽  
Survival Rates ◽  
Pcr Analysis ◽  
Antigen Delivery ◽  
Strong Activity ◽  
Western Blot Method ◽  
Cytokine Analysis ◽  
Virus Like Particle ◽  
Potential Vaccine

This study was carried out to evaluate the vaccination effect of a virus-like particle (VLP) including the surface antigen 1 (SAG1) of Toxoplasma gondii as a potential vaccine for toxoplasmosis. The SAG1 virus-like particles (SAG1-VLPs) were expressed by Sf9 cells, and their expression was confirmed through cloning, RT-PCR analysis, and western blot method. The immunogenicity and vaccine efficacy of SAG1-VLPs were assessed by the antibody response, cytokine analysis, neutralization activity, splenocyte assay, and survival rates through a mouse model. In particular, IgG, IgG1, IgG2a, and IgA were markedly increased after immunization, and the survival rates of T. gondii were strongly inhibited by the immunized sera. Furthermore, the immunization of SAG1-VLPs effectively decreased the production of specific cytokines, such as IL-1β, IL-6, TNF-α, and IFN-γ, after parasite infection. In particular, the immunized group showed strong activity and viability compared with the non-immunized infection group, and their survival rate was 75%. These results demonstrate that SAG1-VLP not only has the immunogenicity to block T. gondii infection by effectively inducing the generation of specific antibodies against T. gondii, but is also an effective antigen delivery system for preventing toxoplasmosis. This study indicates that SAG1-VLP can be effectively utilized as a promising vaccine candidate for preventing or inhibiting T. gondii infection.

scholarly journals A selector of transcription initiation in the protozoan parasite Toxoplasma gondii.

1995 ◽  
Vol 15 (1) ◽  
pp. 87-93 ◽  
Author(s):  
D Soldati ◽  
J C Boothroyd
Keyword(s):  
Toxoplasma Gondii ◽  
Gene Transcription ◽  
Transcription Initiation ◽  
Protozoan Parasite ◽  
Intracellular Parasite ◽  
Transcription Start ◽  
Transcription Start Sites ◽  
Obligate Intracellular ◽  
Obligate Intracellular Parasite ◽  
Sag1 Gene

The recent development of an efficient transfection system for the apicomplexan Toxoplasma gondii allows a comprehensive dissection of the elements involved in gene transcription in this obligate intracellular parasite. We demonstrate here that for the SAG1 gene, a stretch of six repeated sequences in the region 35 to 190 bp upstream of the first of two transcription start sites is essential for efficient and accurate transcription initiation. This repeat element shows characteristics of a selector in determining the position of the transcription start sites.

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