scholarly journals Comparison of Liver Extract Medium With Novy-MacNeal-Nicolle Medium and the Molecular Method for the Diagnosis of Leishmaniasis

2020 ◽  
Vol 33 (2) ◽  
pp. 137-141
Author(s):  
Ahmet Ozbilgin ◽  
◽  
Ozlem Tunger ◽  
Isil Inanir ◽  
Ibrahim Cavus ◽  
...  
Keyword(s):  
Liver Extract ◽  
Molecular Method ◽  
Extract Medium

scholarly journals DEVELOPMENT AND PATHOGENICITY OF INFECTIVE JUVENILES ORIGINATING VIA ENDOTOKIA MATRICIDA IN AXENIC STEINERNEMATID NEMATODES

2008 ◽  
Vol 14 (1) ◽  
pp. 7-13
Author(s):  
Yuliantoro Baliadi
Keyword(s):  
Insecticidal Activity ◽  
Galleria Mellonella ◽  
Liver Extract ◽  
Axenic Culture ◽  
Chicken Liver ◽  
Monoxenic Culture ◽  
Medium Supplement ◽  
Steinernema Glaseri ◽  
Extract Medium ◽  
Uterine Development

Development and insecticidal activity of axenic infective juveniles (IJs) originating from endotokia matricida in maternal bodies of Steinernema glaseri and S. carpocapsae were investigated with a comparison to IJs developed in monoxenic culture. In comparison with the monoxenic steinernematids, the axenic ones grew slower and produced fewer IJs when they were cultured in a sterile chicken liver extract medium supplement with an autoclaved nematode infected Galleria mellonella larva. The phenomena of endotokia matricida, an intra-uterine development of hatched juvenile, occurred in an axenic culture as did the monoxenic ones. Although it occurred faster in monoxenic culture, the ratio of females bearing endotokia matricida was more numerous in axenic ones. These axenic females also produced IJs through the endotokia matricida phenomenon. Compared to the normal IJs develop in monoxenic culture, the IJs originated via endotokia matricida of axenic nematodes showed lower insecticidal activity and it could not reproduce in G. mellonella cadaver.

Identification Pseudomonas aeruginosa by OprD Gene for Differentiation from Other Pseudomonas Species that Isolated from Clinical Samples

2019 ◽  
Vol 9 (01) ◽  
pp. 46-50
Author(s):  
Ashwak B Al-Hashimy ◽  
Huda S Alagely ◽  
Akeel K Albuaji ◽  
Khalid R Majeed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
General Hospital ◽  
Culture Media ◽  
Final Diagnosis ◽  
Clinical Samples ◽  
Bacterial Isolates ◽  
Molecular Method ◽  
Biochemical Tests ◽  
Pseudomonas Species ◽  
Specific Sequences

The present study included the collection of 100 samples from various clinical sources for investigating the presence of P. aeruginosa in those sources, the samples have been collected from some hospitals in Baghdad and Hillah city (Al-qassim General Hospital, ,Al-hillah teaching hospital,and Al-hashimya General hospital ) which included wounds, burns, ear and sputum infections. The study was carried out through October 2017 till the end of March 2018. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing or number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to P.aeruginosa bacteria during previous tests based on the OprD gene which has specific sequences for P.aeruginosa bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance . The results of the final diagnosis showed that 38 isolates belong to target bacteria were distributed as 18 of burns, 11 isolates of wounds, 6 isolates of ear infection and 3 isolates of sputum, The examination of the sensitivity of all bacterial isolates was done for elected 38 isolation towards the 9 antibiotic by a Bauer - Kirby and the isolates were resistant for a number of antibiotics used such as Ciprofloxacin 65.7%, Norflaxacin 71%, Imipenem 63.1% Meropenem 68.4%, Gentamicin 65.7%, Amikacin 26.3%, Cefepime 68.4%, Ceftazidime 65.7% and Piperacillin 57.8%.Molecular method , All isolates (38) of P. aeruginosa positive for the diagnostic special gene (OprD) genes (100%).

scholarly journals Seewis hantavirus in common shrew (Sorex araneus) in Sweden

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Olivia Wesula Lwande ◽  
Nahla Mohamed ◽  
Göran Bucht ◽  
Clas Ahlm ◽  
Gert Olsson ◽  
...  
Keyword(s):  
Common Shrew ◽  
Hemorrhagic Fever ◽  
Sorex Araneus ◽  
Genome Segment ◽  
Cytochrome C Oxidase I ◽  
Emerging Viruses ◽  
Molecular Method ◽  
Typing Method ◽  
Common Shrew Sorex Araneus ◽  
Genetic Assay

Abstract Background Rodent borne hantaviruses are emerging viruses infecting humans through inhalation. They cause hemorrhagic fever with renal syndrome and hemorrhagic cardiopulmonary syndrome. Recently, hantaviruses have been detected in other small mammals such as Soricomorpha (shrews, moles) and Chiroptera (bats), suggested as reservoirs for potential pandemic viruses and to play a role in the evolution of hantaviruses. It is important to study the global virome in different reservoirs, therefore our aim was to investigate whether shrews in Sweden carried any hantaviruses. Moreover, to accurately determine the host species, we developed a molecular method for identification of shrews. Method Shrews (n = 198), caught during 1998 in Sweden, were screened with a pan-hantavirus PCR using primers from a conserved region of the large genome segment. In addition to morphological typing of shrews, we developed a molecular based typing method using sequencing of the mitochondrial cytochrome C oxidase I (COI) and cytochrome B (CytB) genes. PCR amplified hantavirus and shrew fragments were sequenced and phylogenetically analysed. Results Hantavirus RNA was detected in three shrews. Sequencing identified the virus as Seewis hantavirus (SWSV), most closely related to previous isolates from Finland and Russia. All three SWSV sequences were retrieved from common shrews (Sorex araneus) sampled in Västerbotten County, Sweden. The genetic assay for shrew identification was able to identify native Swedish shrew species, and the genetic typing of the Swedish common shrews revealed that they were most similar to common shrews from Russia. Conclusion We detected SWSV RNA in Swedish common shrew samples and developed a genetic assay for shrew identification based on the COI and CytB genes. This was the first report of presence of hantavirus in Swedish shrews.

Isolation and identification of N2-fixing Pseudomonas associated with wetland rice

1983 ◽  
Vol 29 (8) ◽  
pp. 867-873 ◽  
Author(s):  
W. L. Barraquio ◽  
J. K. Ladha ◽  
I. Watanabe
Keyword(s):  
New Species ◽  
Yeast Extract ◽  
Heterotrophic Bacteria ◽  
Fluorescent Antibody ◽  
Wetland Rice ◽  
Isolation And Identification ◽  
Biochemical Characteristics ◽  
Yeast Extract Medium ◽  
Extract Medium ◽  
A New Species

Semisolid yeast extract medium amended with glucose and tryptic soy agar were used to isolate aerobically N2-fixing (C2H2-reducing) heterotrophic bacteria from the root of wetland rice. The isolates were identified as Pseudomonas by gel immunodiffusion and fluorescent antibody techniques in combination with their morphological, cultural, and biochemical characteristics. The N2-fixing H2-utilizing Pseudomonas described in this paper is a new species.

scholarly journals Enzymic degradation of keratosulphates

1970 ◽  
Vol 119 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Michiko Nishida-Fukuda ◽  
Fujio Egami
Keyword(s):  
Column Chromatography ◽  
Liver Extract ◽  
Concerted Action ◽  
Sephadex Column ◽  
Marine Gastropod ◽  
Multienzyme System ◽  
Shark Cartilage

1. A multienzyme system capable of degrading keratosulphates to yield galactose, N-acetylglucosamine and sulphate was found in the liver extract of a marine gastropod, Charonia lampas. 2. During the degradation, neither oligosaccharides nor sulphated sugars were produced. 3. It is suggested that the degradation could be attributed to the concerted action of β-galactosidase, β-N-acetylglucosaminidase and a sulphatase (sulphohydrolase), tentatively designated keratosulphatase. 4. Two forms of keratosulphatase (I and II) were separated by DEAE-Sephadex column chromatography. Both forms could release all the sulphate from keratosulphates and neither appeared to be identical with glycosulphatase or chondrosulphatase, both of which are also present in Charonia lampas. 5. β-Galactosidase and β-N-acetylglucosaminidase could degrade keratopolysulphate to a greater extent in the presence of keratosulphatase than in its absence. 6. It is suggested that keratosulphate was first desulphated by the action of keratosulphatase, and the desulphated polymer was then degraded to galactose and N-acetylglucosamine by the action of β-galactosidase and β-N-acetylglucosaminidase. 7. β-Galactosidase alone released a small amount of galactose from shark cartilage keratopolysulphate, but β-N-acetylglucosaminidase alone did not release N-acetylglucosamine. This indicates that unsulphated galactose residues occupy all the non-reducing terminal positions in keratopolysulphate chains.

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