scholarly journals A Molecularly Imprinted Polymer Based Biosensor for Electrochemical Impedance Spectroscopic Analysis

2018 ◽  
Vol 18 (1) ◽  
pp. 39-44
Author(s):  
Feride Sermin Utku ◽  
◽  
Ozan Enver Ozdemir ◽  
Melahat Sevgul Bakay ◽  
◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Spectroscopic Analysis ◽  
Electrochemical Impedance ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Electrochemical Impedance Spectroscopic

scholarly journals Electrochemical Sensor Based on Molecularly Imprinted Polymer for the Detection of Cefalexin

Biosensors ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 31 ◽  
Author(s):  
Bogdan Feier ◽  
Adrian Blidar ◽  
Alexandra Pusta ◽  
Paula Carciuc ◽  
Cecilia Cristea
Keyword(s):  
Electrochemical Sensor ◽  
Molecularly Imprinted Polymer ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Imprinted Polymer ◽  
Boron Doped Diamond ◽  
Molecularly Imprinted ◽  
Diamond Electrode ◽  
The Difference ◽  
Mip Sensor

In this study, a new electrochemical sensor was developed for the detection of cefalexin (CFX), based on the use of a molecularly imprinted polymer (MIP) obtained by electro‒polymerization in an aqueous medium of indole-3-acetic acid (I3AA) on a glassy carbon electrode (GCE) and on boron-doped diamond electrode (BDDE). The two different electrodes were used in order to assess how their structural differences and the difference in the potential applied during electrogeneration of the MIP translate to the performances of the MIP sensor. The quantification of CFX was performed by using the electrochemical signal of a redox probe before and after the rebinding of the template. The modified electrode was characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The influence of different parameters on the fabrication of the sensor was tested, and the optimized method presented high selectivity and sensitivity. The MIP-based electrode presented a linear response for CFX concentration range of 10 to 1000 nM, and a limit of detection of 3.2 nM and 4.9 nM was obtained for the BDDE and the GCE, respectively. The activity of the sensor was successfully tested in the presence of some other cephalosporins and of other pharmaceutical compounds. The developed method was successfully applied to the detection of cefalexin from real environmental and pharmaceutical samples.

An electrochemical sensor for the determination of phoxim based on a graphene modified electrode and molecularly imprinted polymer

2015 ◽  
Vol 7 (11) ◽  
pp. 4786-4792 ◽  
Author(s):  
Xuecai Tan ◽  
Jiawen Wu ◽  
Qi Hu ◽  
Xiaoyu Li ◽  
Pengfei Li ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Modified Electrode ◽  
Molecularly Imprinted Polymer ◽  
Electrochemical Impedance ◽  
Impedance Spectra ◽  
Imprinted Polymer ◽  
Electrochemical Impedance Spectra ◽  
Molecularly Imprinted

The electrochemical impedance spectra of Fe(CN)63−/4− at NIP/graphene/GCE (a), MIP/GCE (b), the bare GCE (c), and MIP/graphene/GCE (d).

scholarly journals Novel Electrochemical Molecularly Imprinted Polymer-Based Biosensor for Tau Protein Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 238
Author(s):  
Amira Ben Hassine ◽  
Noureddine Raouafi ◽  
Felismina T. C. Moreira
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Tau Protein ◽  
Electrochemical Impedance ◽  
Protein A ◽  
Protein Detection ◽  
Tau Proteins ◽  
Proteinase K ◽  
Imprinted Polymer ◽  
Serum Samples ◽  
Molecularly Imprinted

A novel electrochemical biosensor based on a molecularly imprinted polymer (MIP) was developed for the impedimetric determination of Tau protein, a biomarker of Alzheimer’s disease (AD). Indeed, a recent correlation between AD symptoms and the presence of Tau proteins in their aggregated form made hyperphosphorylated Tau protein (Tangles) a promising biomarker for Alzheimer’s diagnosis. The MIP was directly assembled on a screen-printed carbon electrode (C-SPE) and prepared by electropolymerization of 3-aminophenol (AMP) in the presence of the protein template (p-Tau-441) using cyclic voltammetry. The p-Tau-441 protein bound to the polymeric backbone was digested by the action of the proteolytic activity of proteinase K in urea and then washed away to create vacant sites. The performances of the corresponding imprinted and non-imprinted electrodes were evaluated by electrochemical impedance spectroscopy. The detection limit of the MIP-based sensors was 0.02 pM in PBS buffer pH 5.6. Good selectivity and good results in serum samples were obtained with the developed platform. The biosensor described in this work is a potential tool for screening Tau protein on-site and an attractive complement to clinically established methodologies methods as it is easy to fabricate, has a short response time and is inexpensive.

Liquid Chromatography Analysis of Clozapine in Rat Brain Tissue, Using its Molecularly Imprinted Polymer after Administration of Toxic Dose of Drug and Lipid Emulsion Therapy

2019 ◽  
Vol 15 (3) ◽  
pp. 251-257
Author(s):  
Bahareh Sadat Yousefsani ◽  
Seyed Ahmad Mohajeri ◽  
Mohammad Moshiri ◽  
Hossein Hosseinzadeh
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Molecularly Imprinted Polymer ◽  
Lipid Emulsion ◽  
Limit Of Detection ◽  
Imprinted Polymer ◽  
Toxic Dose ◽  
Molecularly Imprinted ◽  
The Brain ◽  
Rat Brain Tissue

Background:Molecularly imprinted polymers (MIPs) are synthetic polymers that have a selective site for a given analyte, or a group of structurally related compounds, that make them ideal polymers to be used in separation processes.Objective:An optimized molecularly imprinted polymer was selected and applied for selective extraction and analysis of clozapine in rat brain tissue.Methods:A molecularly imprinted solid-phase extraction (MISPE) method was developed for preconcentration and cleanup of clozapine in rat brain samples before HPLC-UV analysis. The extraction and analytical process was calibrated in the range of 0.025-100 ppm. Clozapine recovery in this MISPE process was calculated between 99.40 and 102.96%. The limit of detection (LOD) and the limit of quantification (LOQ) of the assay were 0.003 and 0.025 ppm, respectively. Intra-day precision values for clozapine concentrations of 0.125 and 0.025 ppm were 5.30 and 3.55%, whereas inter-day precision values of these concentrations were 9.23 and 6.15%, respectively. In this study, the effect of lipid emulsion infusion in reducing the brain concentration of drug was also evaluated.Results:The data indicated that calibrated method was successfully applied for the analysis of clozapine in the real rat brain samples after administration of a toxic dose to animal. Finally, the efficacy of lipid emulsion therapy in reducing the brain tissue concentration of clozapine after toxic administration of drug was determined.Conclusion:The proposed MISPE method could be applied in the extraction and preconcentration before HPLC-UV analysis of clozapine in rat brain tissue.

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