scholarly journals Predictive Role of Ki-67 and Proliferative-Cell Nuclear Antigen (PCNA) in Recurrent Cholesteatoma

2019 ◽  
Vol 15 (1) ◽  
pp. 38-42 ◽  
Author(s):  
Ela Araz Server ◽  
◽  
Cigdem Kalaycik Ertugay ◽  
Sevim Baykal Koca ◽  
Ecem Sevim Longur ◽  
...  
Keyword(s):  
Nuclear Antigen ◽  
Proliferative Cell Nuclear Antigen ◽  
Ki 67 ◽  
Predictive Role ◽  
Proliferative Cell

scholarly journals Cholangiocarcinoma in a Ring-Necked Pheasant (Phasianus colchicus): a case report

2012 ◽  
Vol 57 (No. 6) ◽  
pp. 320-323 ◽  
Author(s):  
O. Ozmen
Keyword(s):  
Case Report ◽  
Connective Tissue ◽  
Liver Parenchyma ◽  
Tumour Cells ◽  
Nuclear Antigen ◽  
Phasianus Colchicus ◽  
Proliferative Cell Nuclear Antigen ◽  
Ki 67 ◽  
First Report ◽  
Proliferative Cell

A case of cholangiocarcinoma in a two-year-old Ring-Necked Pheasant (Phasianus colchicus) is reported. The liver was enlarged and numerous white foci were seen in the liver parenchyma. Histopathologically, channels with or without lumens and separated from one another by thin connective tissue septa were observed. Tumour cells were strongly positive for cytokeratin, Ki-67 and proliferative cell nuclear antigen. This is the first report of colangiocarcinoma in pheasants.  

Expression of Ki-67 antigen and proliferative cell nuclear antigen in benign and malignant epithelial lesions of the larynx

1996 ◽  
Vol 110 (5) ◽  
pp. 440-445 ◽  
Author(s):  
Nina Zidar ◽  
Nina Gale ◽  
Andrej Cör ◽  
Vinko Kambič
Keyword(s):  
Basal Layer ◽  
Histologic Grade ◽  
Nuclear Antigen ◽  
Severe Dysplasia ◽  
Epithelial Hyperplasia ◽  
Proliferative Cell Nuclear Antigen ◽  
Ki 67 ◽  
Histological Techniques ◽  
Epithelial Lesions ◽  
Proliferative Cell

AbstractIn an attempt to analyse the proliferative activity in benign and malignant laryngeal epithelial lesions, and to determine the relationship to their histologic grade, we studied the expression of proliferative cell nuclear antigen (PCNA) and Ki-67 antigen on 20 squamous carcinomas, and on 30 biopsies of epithelial hyperplasia categorized according to the Kambiˇ-Lenart classification into simple, abnormal, and atypical hyperplasias. In simple hyperplasia, both antibodies stained the nuclei of the occasional cells in the basal layer. In abnormal hyperplasia (mild dysplasia), positive cells occupied up to a third, and in atypical hyperplasia (moderate and severe dysplasia) they occupied from two-thirds to the entire epithelial thickness. In squamous carcinoma, we have found a statistically significant correlation between its grade and the percentage of Ki-67-(p<0.01) and PCNA-(p<0.00001) positive cells. Our results suggest that the proliferative fraction progressively increases with the degree of epithelial hyperplasia and the grade of carcinoma. We conclude that the patterns of immunoreactivity to PCNA and Ki-67 antigen correspond to the histologic grade of both benign and malignant epithelial lesions of the larynx. This method should be regarded as a useful adjunct to traditional histological techniques allowing more objective grading of benign and malignant epithelial lesions.

scholarly journals Urocortin-dependent effects on adrenal morphology, growth, and expression of steroidogenic enzymes in vivo

2012 ◽  
Vol 48 (2) ◽  
pp. 159-167 ◽  
Author(s):  
Anna Riester ◽  
Ariadni Spyroglou ◽  
Adi Neufeld-Cohen ◽  
Alon Chen ◽  
Felix Beuschlein
Keyword(s):  
Adrenal Gland ◽  
Stress Responses ◽  
Indirect Evidence ◽  
Nuclear Antigen ◽  
Proliferative Cell Nuclear Antigen ◽  
Organ Systems ◽  
And Function ◽  
Proliferative Cell

Urocortin (UCN) 1, 2, and 3 are members of the corticotropin-releasing factor (CRF) family that display varying affinities to the CRF receptor 1 (CRFR1 (CRHR1)) and 2 (CRFR2 (CRHR2)). UCNs represent important modulators of stress responses and are involved in the control of anxiety and related disorders. In addition to the CNS, UCNs and CRFRs are highly expressed in several tissues including the adrenal gland, indicating the presence of UCN-dependent regulatory mechanisms in these peripheral organ systems. Using knockout (KO) mouse models lacking single or multipleUcngenes, we examined the potential role of the three differentUcns on morphology and function of the adrenal gland. Adrenal morphology was investigated, organ size, cell size, and number were quantified, and growth kinetics were studied by proliferative cell nuclear antigen staining andCcnd1expression analysis. Furthermore, mRNA expression of enzymes involved in steroidogenesis and catecholamine synthesis was quantified by real-time PCR. Following this approach,Ucn2,Ucn1/Ucn2dKO andUcn1/Ucn2/Ucn3tKO animals showed a significant cellular hypotrophy of the adrenal cortex and an increase inCcnd1expression, whereas in all other genotypes, no changes were observable in comparison to age-matched controls. For steroidogenesis,Ucn2/Ucn3dKO animals displayed the most pronounced changes, with significant increases in all investigated enzymes, providing indirect evidence for increased stress behavior. Taken together, these data suggest that mainlyUcn2andUcn3could be involved in adrenal stress response regulation whileUcn2additionally appears to play a role in morphology and growth of the adrenal gland.

scholarly journals Phloretin Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by Regulating the Inflammatory Response, Oxidative Stress and Apoptosis

Life ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 743
Author(s):  
Chao Yu Hsu ◽  
Yi Sheng Lin ◽  
Wei Chun Weng ◽  
Lauren Panny ◽  
Hsiang Lai Chen ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Weight Ratio ◽  
Nuclear Antigen ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Body Weight Ratio ◽  
Proliferative Cell Nuclear Antigen ◽  
Prostate Weight ◽  
Prostatic Enlargement ◽  
Inflammatory Proteins ◽  
Proliferative Cell

The inflammatory process is proposed to be one of the factors to benign prostatic enlargement (BPH), and this is the first study examining the anti-inflammatory ability of phloretin in treating rats with testosterone-induced BPH. BPH would be induced by testosterone (10 mg/kg/day testosterone subcutaneously for 28 days), and the other groups of rats were treated with phloretin 50 mg/kg/day or 100 mg/kg/day orally (phr50 or phr100 group) after induction. Prostate weight and prostate weight to body weight ratio were significantly reduced in the Phr100 group. Reduced dihydrotestosterone without interfering with 5α-reductase was observed in the phr100 group. In inflammatory proteins, reduced IL-6, IL-8, IL-17, NF-κB, and COX-2 were seen in the phr100 group. In reactive oxygen species, malondialdehyde was reduced, and superoxide dismutase and glutathione peroxidase were elevated in the phr100 group. In apoptotic assessment, elevated cleaved caspase-3 was observed in rats of the phr100 group. Enhanced pro-apoptotic Bax and reduced anti-apoptotic Bc1-2 could be seen in the phr100 group. In histological stains, markedly decreased glandular hyperplasia and proliferative cell nuclear antigen were observed with reduced expression in the phr100 group. Meanwhile, positive cells of terminal deoxynucleotidyl transferase dUTP nick end labeling were increased in the phr100 group. In conclusion, the treatment of phloretin 100 mg/kg/day could ameliorate testosterone-induced BPH.

scholarly journals PCNA Interacts with Indian Mung Bean Yellow Mosaic Virus Rep and Downregulates Rep Activity

2004 ◽  
Vol 78 (21) ◽  
pp. 11890-11903 ◽  
Author(s):  
Basavaraj Bagewadi ◽  
Shoajiang Chen ◽  
Sunil K. Lal ◽  
Nirupam Roy Choudhury ◽  
Sunil K. Mukherjee
Keyword(s):  
Mosaic Virus ◽  
Mung Bean ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Nuclear Antigen ◽  
Resting Cells ◽  
Rolling Circle Replication ◽  
Proliferative Cell Nuclear Antigen ◽  
Bipartite Genome

ABSTRACT Proliferative cell nuclear antigen (PCNA), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. The biochemical role of PCNA in rolling circle replication (RCR) of geminivirus DNA has not been explored in detail. The initiation of RCR of the bipartite genome of a geminivirus, Indian mung bean yellow mosaic virus (IMYMV), is mainly controlled by viral protein Rep (or AL1 or AC1). The role of host PCNA in RCR of IMYMV was revealed by studying the physical and functional interactions between recombinant PCNA and recombinant IMYMV Rep. Pea nuclear PCNA as well as recombinant pea PCNA showed binding to recombinant Rep in experiments involving both affinity chromatography and yeast two-hybrid approaches. The contacting amino acid residues of PCNA seemed to be present throughout a wide region of the trimeric protein, while those of Rep appeared to be localized only in the middle part of the protein. The site-specific nicking-closing activity and the ATPase function of IMYMV Rep were impaired by PCNA. These observations lead to interesting speculations about the control of viral RCR and dynamic profiles of protein-protein interactions at the RCR origin of the geminiviruses.

scholarly journals Cyanidin-3-O-Glucoside-Rich Haskap Berry Administration Suppresses Carcinogen-Induced Lung Tumorigenesis in A/JCr Mice

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3823 ◽  
Author(s):  
Madumani Amararathna ◽  
David W. Hoskin ◽  
H. P. Vasantha Rupasinghe
Keyword(s):  
Lung Tumor ◽  
Immunohistochemical Analysis ◽  
Lung Tumors ◽  
Lung Epithelial Cells ◽  
Nuclear Antigen ◽  
Normal Lung ◽  
Lung Tumorigenesis ◽  
Proliferative Cell Nuclear Antigen ◽  
Ki 67

In our previous study, we demonstrated that cyanidin-3-O-glucoside (C3G)-rich haskap (Lonicera caerulea L.) berry extracts can attenuate the carcinogen-induced DNA damage in normal lung epithelial cells in vitro. Here, the efficacy of lyophilized powder of whole haskap berry (C3G-HB) in lowering tobacco-specific nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, (NNK)-induced lung tumorigenesis in A/JCr mice was investigated. Three weeks after daily oral administration of C3G-HB (6 mg of C3G in 0.2 g of C3G-HB/mouse/day), lung tumors were initiated by a single intraperitoneal injection of NNK. Dietary C3G-HB supplementation was continued, and 22 weeks later, mice were euthanized. Lung tumors were visualized through positron emission tomography (PET) and magnetic resonance imaging (MRI) 19 weeks after NNK injection. Dietary supplementation of C3G-HB significantly reduced the NNK-induced lung tumor multiplicity and tumor area but did not affect tumor incidence. Immunohistochemical analysis showed reduced expression of proliferative cell nuclear antigen (PCNA) and Ki-67 in lung tissues. Therefore, C3G-HB has the potential to reduce the lung tumorigenesis, and to be used as a source for developing dietary supplements or nutraceuticals for reducing the risk of lung cancer among high-risk populations.

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