scholarly journals In-vivo and In-Vitro Examination of the Effect of Lucilia Sericata Larvae and Secretions on the Bacteria in Open Wounds

2020 ◽  
Vol 5 (2) ◽  
pp. 113-116
Author(s):  
Erdal Polat ◽  
◽  
Dilek Bolaban ◽  
Serhat Sirekbasan ◽  
◽  
...  
Keyword(s):  
Lucilia Sericata ◽  
In Vitro Examination

In vivo and in vitro Examination of the Tendinous Inscription of the Human Semitendinosus Muscle

2012 ◽  
Vol 195 (4) ◽  
pp. 365-376 ◽  
Author(s):  
Eleftherios Kellis ◽  
Nikiforos Galanis ◽  
Konstantinos Natsis ◽  
George Kapetanos
Keyword(s):  
Semitendinosus Muscle ◽  
In Vitro Examination

Diameter and Pulsatile Oscillations of the Femoral Artery Recorded by Ultrasound

1984 ◽  
Vol 25 (4) ◽  
pp. 313-316 ◽  
Author(s):  
T. Christensen ◽  
J. Jørgensen ◽  
B. Neubauer
Keyword(s):  
Arterial Wall ◽  
External Iliac Artery ◽  
Non Invasive ◽  
Time Motion ◽  
Invasive Method ◽  
Inner Diameter ◽  
In Vitro Examination

A new non-invasive method for in vivo investigations of the inner diameter and pulsatile oscillations of large muscular arteries by means of an ultrasound time-motion technique is presented. Each histologic layer of the arterial wall is identified at in vitro examination of the external iliac artery. The method has been used for in vivo investigations of 16 long term diabetics and of 16 non-diabetics. A reduction of the lumen and decrement in pulse deflections were found in the diabetics when compared with those of the normal group.

Cardiovascular collapse induced by Echis ocellatus venom: an in vivo and in vitro examination

Toxicon ◽  
2019 ◽  
Vol 159 ◽  
pp. S14-S15
Author(s):  
Rahini Ragavan ◽  
Anjana Silva ◽  
Barbara Kemp-Harper ◽  
Sanjaya Kuruppu ◽  
Geoffrey K. Isbister ◽  
...  
Keyword(s):  
Cardiovascular Collapse ◽  
In Vitro Examination

In vitro analyses of cuticular differentiation at the chromosomal level in Lucilia sericata

Genome ◽  
1988 ◽  
Vol 30 (4) ◽  
pp. 603-607 ◽  
Author(s):  
Manfred G. Schmiemann ◽  
Michael M. Bentley1
Keyword(s):  
Culture Medium ◽  
Polytene Chromosomes ◽  
Culture Conditions ◽  
Lucilia Sericata ◽  
In Vitro Cultivation ◽  
Pupal Development ◽  
Chromosome Conformation ◽  
Tormogen Cell

The large bristles of flies sclerotize and melanize during the pupal phase well before the rest of the cuticle. Each bristle is the product of two cells, the trichogen cell and the tormogen cell. Both their nuclei exhibit analyzable polytene chromosomes. The pupal metathoracic integument of Lucilia sericata has been excised and cultivated in Schneider's Drosophila medium for extended periods of time. Among the differentiations taking place in vitro, the most obvious is the stiffening and coloring of the bristles. The in vitro differentiation very much resembles the in vivo differentiation in its timing and appearance. Explants excised early in pupal development cannot differentiate in vitro, while those excised and cultured at later stages can. Actinomycin D was administered to the culture medium and the incorporation of [3H]uridine was analyzed after long incubations to determine if this process was a reaction of dead cuticle or controlled by living cells. The analysis of chromosome structure and puffing patterns in the five polytene chromosomes of the trichogen cell after in vitro cultivation shows that transcriptional activity is not dependent on the existence of normal chromosome conformation. The degree of pycnotic reaction and puff induction of the nuclei, however, varied under different culture conditions. The capacity of this system for differentiation can be used to study in vitro chromosomal activity during the course of development.Key words: in vitro, polytene chromosomes, sclerotization, cuticle.

Detection of anti-leishmanial effect of the Lucilia sericata larval secretions in vitro and in vivo on Leishmania tropica: First work

2012 ◽  
Vol 132 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Erdal Polat ◽  
Huseyin Cakan ◽  
Mustafa Aslan ◽  
Serhat Sirekbasan ◽  
Zekayi Kutlubay ◽  
...  
Keyword(s):  
Lucilia Sericata ◽  
Leishmania Tropica

scholarly journals The therapeutic effects of larval excretion/secretion of Lucilia sericata on Leishmania major under in vitro and in vivo conditions

2021 ◽  
Author(s):  
Jila Sherafati ◽  
Mohammad Saaid Dayer ◽  
Fatemeh Ghaffarifar
Keyword(s):  
Leishmania Major ◽  
Skin Lesions ◽  
Lesion Size ◽  
Negative Control ◽  
Lucilia Sericata ◽  
Definitive Treatment ◽  
Therapeutic Effects ◽  
Model Animal

Abstract Background Leishmaniasis is a neglected infectious disease caused by a kinetoplastid protozoan. The disease generally manifests as characteristic skin lesions. Due to the lack of definitive treatment and drugs without side effects, many studies have focused on natural compounds as promising drugs for its treatment. This study aimed to evaluate the effects of larval excretion/secretion products (ES) of Lucilia sericata in crude and fractionated forms on Leishmania parasites under both in vitro and in vivo conditions. Methods In vitro experiments involved evaluation of ES products on both promastigotes and amastigotes inside infected macrophages, whereas in vivo experiments included comparative treatments of Leishmanial lesions of mice using Eucerin-formulated ES products and glucantime. Results The IC50 values were 38.7 µg/ml, 47.6 µg/ml, 63.3 µg/ml, and 29.1 µg/ml for crude ES, over 10 kDa ES-fraction, under 10 kDa ES-fraction, and glucantime respectively. Significant differences were observed between viability percentages of promastigotes treated with crude ES and its fractions compared to negative control (p < 0.0001). Crude ES was more effective on amastigote than other two ES fractions at 300 µg/ml concentration. Macroscopic measurement of lesion sizes revealed that the reduction of lesion size in mice treated with crude ES followed quicker cascades of healing than in those treated with glucantime and fractionated ES. Conclusion The present study showed that larval ES of Lucilia sericata in both crude and fractionated forms are effective on both intracellular and extracellular forms of L. major. It also provided evidence that the larval ES exerts both topical and systemic therapeutic effects on leishmanial lesions of the model animal.

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