scholarly journals Using Quick Test, California Mastitis Test, and Somatic Cell Count for Diagnosis of Subclinical Mastitis Related with Human Health Risk

2019 ◽  
Vol 3 (3) ◽  
pp. 154-158
Author(s):  
Isfendiyar Darbaz ◽  
◽  
Osman Ergene ◽  
Keyword(s):  
Health Risk ◽  
Somatic Cell ◽  
Cell Count ◽  
Human Health ◽  
Somatic Cell Count ◽  
Human Health Risk ◽  
Subclinical Mastitis ◽  
Quick Test ◽  
California Mastitis Test

A COMPARISON OF MASTITIS DETECTION METHODS IN DAIRY CATTLE

1984 ◽  
Vol 64 (2) ◽  
pp. 305-312 ◽  
Author(s):  
T. R. BATRA ◽  
A. J. McALLISTER
Keyword(s):  
Dairy Cattle ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Good Accuracy ◽  
Subclinical Mastitis ◽  
Detection Methods ◽  
Considerable Proportion ◽  
Milk Samples ◽  
California Mastitis Test

The potential value of electrical conductivity, somatic cell count, and California Mastitis Test (CMT) Score in composite milk samples as a screening test for subclinical mastitis was evaluated. The effect of genetic group was significant (P < 0.05) for conductivity and somatic cell count while the effects of parity and month of lactation were significant (P < 0.01) for all the three traits studied. Somatic cell count and CMT score increased from first to fourth parity while there was general trend for increasing conductivity from second to fourth parity. All the three methods identified healthy cows with a good accuracy but failed to recognize a considerable proportion of infected cows. The error rate for misclassification was lowest for CMT score (9.1%), followed by somatic cell count (13.9%) and conductivity (29.4%). Composite strict foremilk samples from cows infected with primary pathogens had significantly (P < 0.01) higher conductivity than those from uninfected cows. Staphylococcus aureus were isolated in 60% of the milk samples and appeared to be in the major organism causing mastitis in this herd. Key words: Dairy cattle, mastitis detection methods

Evaluation of the udder health status in subclinical mastitis affected dairy cows through bacteriological culture, somatic cell count and thermographic imaging

2015 ◽  
Vol 18 (4) ◽  
pp. 799-805 ◽  
Author(s):  
A. Bortolami ◽  
E. Fiore ◽  
M. Gianesella ◽  
M. Corrò ◽  
S. Catania ◽  
...  
Keyword(s):  
Health Status ◽  
Dairy Cows ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Subclinical Mastitis ◽  
Bacteriological Culture ◽  
Udder Health ◽  
Streptococcus Uberis ◽  
Thermographic Imaging

Abstract Subclinical mastitis in dairy cows is a big economic loss for farmers. The monitoring of subclinical mastitis is usually performed through Somatic Cell Count (SCC) in farm but there is the need of new diagnostic systems able to quickly identify cows affected by subclinical infections of the udder. The aim of this study was to evaluate the potential application of thermographic imaging compared to SCC and bacteriological culture for infection detection in cow affected by subclinical mastitis and possibly to discriminate between different pathogens. In this study we evaluated the udder health status of 98 Holstein Friesian dairy cows with high SCC in 4 farms. From each cow a sample of milk was collected from all the functional quarters and submitted to bacteriological culture, SCC and Mycoplasma spp. culture. A thermographic image was taken from each functional udder quarter and nipple. Pearson’s correlations and Analysis of Variance were performed in order to evaluate the different diagnostic techniques. The most frequent pathogen isolated was Staphylococcus aureus followed by Coagulase Negative Staphylococci (CNS), Streptococcus uberis, Streptococcus agalactiae and others. The Somatic Cell Score (SCS) was able to discriminate (p<0.05) cows positive for a pathogen from cows negative at the bacteriological culture except for cows with infection caused by CNS. Infrared thermography was correlated to SCS (p<0.05) but was not able to discriminate between positive and negative cows. Thermographic imaging seems to be promising in evaluating the inflammation status of cows affected by subclinical mastitis but seems to have a poor diagnostic value.

scholarly journals Bulk tank somatic cell count and associated microbial quality of milk from selected dairy cattle herds in Oyo State, Nigeria

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Olufemi Olatoye ◽  
Adesola Amosun ◽  
Uzo Ogbu ◽  
Yemi Okunlade
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Health Management ◽  
Development Program ◽  
Raw Milk ◽  
Human Consumption ◽  
Quality And Safety ◽  
Milk Samples ◽  
California Mastitis Test

Improvement of traditional and nomadic milk production through dairy development program in Nigeria requires routine quality and safety monitoring of milk both at herd level and milk collection centers. A total of 411 bulk raw milk samples aseptically obtained from Ibarapa, Oyo and Oke-Ogun industrial milk collection centers were subjected to California Mastitis Test (CMT), Bulk Somatic Cell Count (BSCC) and bacteriological analysis for assessment of quality and safety of milk from the herds. One hundred and seven (26.0%) of the samples were CMT positive, while 74.0% were negative to CMT. The overall mean BSCC, TAC and TCC were 1.27×103 ± cells/mL, 1.12×103± 34 cfu/mL, 97.8±9.8 cfu/mL in the CMT negative milk samples while for the strong positive samples the mean BSCC, TAC and TCC were 4.33×106 ± cells/mL, 2.35×106 ± 453 cfu/mL, 189.3±41.1 cfu/mL respectively; these were higher than the Pasteurized Milk Ordinance acceptable limits. Positive correlation was found between CMT scores and bacterial contamination and between CMT scores and SCC was recorded. About 26.0% of the samples with positive CMT could be considered unsafe due to strong correlation with microbial contamination that could result in milk borne zoonoses and public health hazards. However, a greater proportion (76.9%) of the milk with negative CMT scores could be safe for human consumption after post-harvest pasteurization. Consequently, there is need to improve handling, environmental and milking hygiene; as well as proper herd and udder health management to improve quality and safety of Nigeria dairy products.

scholarly journals Threshold somatic cell count for delineation of subclinical mastitis cases

2018 ◽  
Vol 11 (6) ◽  
pp. 789-793 ◽  
Author(s):  
P. V. Jadhav ◽  
D. N. Das ◽  
K. P. Suresh ◽  
B. R. Shome
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Subclinical Mastitis

scholarly journals The presence of pathogens in milk of ewes in relation to the somatic cell count and subpopulations of leukocytes

2021 ◽  
Author(s):  
Tvarožková ◽  
Vašíček ◽  
Uhrinčať ◽  
Mačuhová ◽  
Hleba ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Dairy Farm ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Polymorphonuclear Cells ◽  
Coagulase Negative Staphylococci ◽  
Major Health Problem ◽  
Milk Samples

Mastitis is a major health problem of the udder in dairy sheep breeds. For diagnosis of subclinical mastitis, somatic cell count (SCC) is commonly used. The presence of pathogens in the udder causes the increase of leukocytes and thus SCC in milk. Therefore, the aim of this study was to evaluate the presence of pathogens in the milk of ewes and the possible relationship with SCC. The changes of leukocytes subpopulation in milk samples with high SCC were evaluated as well. The experiment was carried out on a dairy farm with the Lacaune breed. This study was conducted on 45 ewes (98 milk samples) without signs of clinical mastitis. Based on somatic cell count, samples were divided to five SCC groups: SCC1 &lt; 200 000 cells/ml (45 milk samples); 200 000 ≤ SCC2 &lt; 400 000 cells/ml (10 milk samples); 400 000 ≤ SCC3 &lt; 600 000 cells/ml (six milk samples); 600 000 ≤ SCC4 &lt; 1 000 000 cells/ml (six milk samples); SCC5 ≥ 1 000 000 cells/ml (31 milk samples). No pathogens were observed in the majority of milk samples (60.20%). Coagulase-negative staphylococci (CNS) were the most commonly isolated pathogens from the milk of ewes (86.11%). Staphylococcus epidermidis had the highest incidence from CNS (35.48%). In the SCC5 group, up to 79.31% of bacteriological samples were positive. The percentage of leukocytes significantly increased (P &lt; 0.001) in the samples with higher SCC (≥ 200 × 10<sup>3</sup> cells/ml) in comparison to the group SCC1. Also, the percentage of polymorphonuclear cells (PMNs) was significantly higher with increasing SCC (P &lt; 0.001). In conclusion, the presented results showed that the high SCC was caused by the presence of the pathogen in milk. Thus SCC &lt; 200 000 cells/ml and leukocyte subpopulation, especially PMNs, could be considered as important tools in udder health programs applied in dairy ewes.

scholarly journals Somatic cell count in the diagnosis of subclinical mastitis in sheep of different breeds

2018 ◽  
Vol 39 (4) ◽  
pp. 1555
Author(s):  
Luiz Francisco Zafalon ◽  
Raul Costa Mascarenhas Santana ◽  
Sérgio Novita Esteves ◽  
Guilherme Aparecido Fim Júnior
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Subclinical Mastitis ◽  
Control Measures ◽  
Coagulase Negative Staphylococci ◽  
Stage Of Lactation ◽  
Santa Inês ◽  
Etiological Agents

The aims of this study were to determine the occurrence of subclinical mastitis in sheep of different breeds and the values for somatic cell count (SCC) in milk for the diagnosis of the disease at lactation and weaning, a fundamental prerequisite for identifying animals in need of control measures. Milk samples were obtained from 1,457 mammary halves of Santa Inês, Texel, Ile de France, and Dorper sheep at two different periods, during the second week of lactation and at weaning. After teats antisepsis, the samples were collected, and identification of the infectious etiology of mastitis and determination of SCC were performed. Microorganisms were identified in 117/762 (15.3%) mammary halves in the second week of lactation and in 86/694 (12.4%) at weaning. Coagulase-negative staphylococci (CoNS) were the etiological agents with the highest incidence alone and in association with other microorganisms, with percentages of 58.1% and 60.6%, respectively. The Santa Inês presented a higher incidence of subclinical mastitis when compared to the other breeds. The cut-off values of SCC for subclinical mastitis were determined at both sampling periods and varied according to stage of lactation, as well breed. These results illustrate the lack of a universal value that can be used for the diagnosis of mastitis and suggests the need for permanent follow-up in herds in order to control the disease.

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