Comparison of ultrasonically activated scalpel and traditional technique in radial artery harvesting; an electron microscopic evaluation

2011 ◽  
Author(s):  
Mert Dumantepe ◽  
Tamer Kehlibar ◽  
A. Umit Gullu ◽  
Yucesin Arslan ◽  
Mehmet Yilmaz ◽  
...  
Keyword(s):  
Radial Artery ◽  
Electron Microscopic ◽  
Ultrasonically Activated Scalpel ◽  
Traditional Technique ◽  
Microscopic Evaluation

Harvesting Techniques Affect the Integrity of the Radial Artery: An Electron Microscopic Evaluation

2004 ◽  
Vol 78 (4) ◽  
pp. 1319-1325 ◽  
Author(s):  
Mustafa Emir ◽  
M.Kamil Gol ◽  
Kanat Ozisik ◽  
Vedat Bakuy ◽  
Mustafa F. Sargon ◽  
...  
Keyword(s):  
Radial Artery ◽  
Electron Microscopic ◽  
Microscopic Evaluation ◽  
Harvesting Techniques

TEM comparison of osmium vs osmium with potassium ferricyanide secondary fixatives and the impact of secondary fixative temperature on tissue preservation/contrast quality

1996 ◽  
Vol 54 ◽  
pp. 910-911
Author(s):  
J. W. Horn ◽  
B. J. Dovey-Hartman ◽  
V. P. Meador
Keyword(s):  
Osmium Tetroxide ◽  
Potassium Ferricyanide ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Microscopic Evaluation ◽  
Cacodylate Buffer ◽  
Transmission Electron Microscopic ◽  
Glutaraldehyde Solution ◽  
Temperature Impact ◽  
The Impact

Osmium tetroxide (OsO4) is a universally used secondary fixative for routine transmission electron microscopic evaluation of biological specimens. Use of OsO4 results in good ultrastructural preservation and electron density but several factors, such as concentration, length of exposure, and temperature, impact overall results. Potassium ferricyanide, an additive used primarily in combination with OsO4, has mainly been used to enhance the contrast of lipids, glycogen, cell membranes, and membranous organelles. The purpose of this project was to compare the secondary fixative solutions, OsO4 vs. OsO4 with potassium ferricyanide, and secondary fixative temperature for determining which combination gives optimal ultrastructural fixation and enhanced organelle staining/contrast.Fresh rat liver samples were diced to ∼1 mm3 blocks, placed into porous processing capsules/baskets, preserved in buffered 2% formaldehyde/2.5% glutaraldehyde solution, and rinsed with 0.12 M cacodylate buffer (pH 7.2). Tissue processing capsules were separated (3 capsules/secondary fixative.solution) and secondarily fixed (table) for 90 minutes. Tissues were buffer rinsed, dehydrated with ascending concentrations of ethanol solutions, infiltrated, and embedded in epoxy resin.

Evaluation of platelet dense granules for determining storage pool deficiencies by HVEM image and quantitative analysis

1996 ◽  
Vol 54 ◽  
pp. 770-771
Author(s):  
W.T. Gunning ◽  
J.N. Turner ◽  
K. Buttle ◽  
E.P. Calomeni ◽  
N.A. Lachant ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Platelet Function ◽  
Electron Microscopic ◽  
Von Willebrand's Disease ◽  
Dense Granules ◽  
Storage Pool ◽  
Prolonged Bleeding ◽  
Microscopic Evaluation ◽  
Storage Pools ◽  
Electron Lucent

There are a variety of conditions which have been associated with prolonged bleeding times. If other etiologies including von Willebrand's disease have been ruled out, a platelet function disorder must be considered. The best, if not only, technique to make this diagnosis is the electron microscopic evaluation of whole air dried platelets. Bull first described the presence of dense granules in whole platelets in 1968 and the technique has been utilized extensively The electron dense or delta granules are easily distinguished from the larger more numerous alpha granules which are electron lucent. The significance of the dense granules is that they are known to be “storage pools” of serotonin, calcium, adenosine di- and triphosphate, and pyrophosphate. Prolonged bleeding times may be directly related to an insufficiency of these substances. The diagnosis of a storage pool deficiency is made when either the storage content of the dense granules is abnormal or their number is diminished. We observe normal platelets to have 4-6 dense granules, which agrees with the literature.

Electron microscopic evaluation of bacterial adherence to polyvinyl chloride endotracheal tubes used in neonates

1989 ◽  
Vol 17 (12) ◽  
pp. 1335-1340 ◽  
Author(s):  
JAVIER DIAZ-BLANCO ◽  
ROBERT C. CLAWSON ◽  
SHIRLEY M. ROBERSON ◽  
CAROL B. SANDERS ◽  
ARUN K. PRAMANIK ◽  
...  
Keyword(s):  
Polyvinyl Chloride ◽  
Bacterial Adherence ◽  
Electron Microscopic ◽  
Endotracheal Tubes ◽  
Microscopic Evaluation
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