scholarly journals Correction: Clinical Evaluation of QMAC-dRAST for Direct and Rapid Antimicrobial Susceptibility Test with Gram-Positive Cocci from Positive Blood Culture Bottles

2018 ◽  
Vol 21 (2) ◽  
pp. 45
Author(s):  
Hyunjung Kim ◽  
Hyun Yong Jeong ◽  
Sangkwon Han ◽  
Shinhun Han ◽  
Jungil Choi ◽  
...  
Keyword(s):  
Blood Culture ◽  
Clinical Evaluation ◽  
Positive Blood Culture ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Test ◽  
Antimicrobial Susceptibility Test ◽  
Gram Positive ◽  
Gram Positive Cocci

scholarly journals A Rapid ATP Bioluminescence-based Test for Detecting Levofloxacin Resistance Starting from Positive Blood Culture Bottles

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Atsushi Matsui ◽  
Hideki Niimi ◽  
Yuichi Uchiho ◽  
Shunsuke Kawabe ◽  
Hideyuki Noda ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Antimicrobial Susceptibility ◽  
Bacterial Suspension ◽  
Antimicrobial Susceptibility Test ◽  
Additional Information ◽  
Atp Bioluminescence ◽  
Antimicrobial Susceptibility Tests ◽  
Turbidity Method ◽  
Susceptibility Tests

Abstract Administering appropriate antimicrobial therapy as early as possible is important for rescuing bacteremic patients. Therefore, rapid antimicrobial susceptibility tests in positive blood culture specimens have been diligently sought. Adenosine triphosphate (ATP) bioluminescence-based methods have been used for rapid antimicrobial susceptibility tests. However, blood culture specimens have not been examined in many studies, possibly due to abundant intracellular ATP in blood corpuscles resulting in false-susceptible results. In this study, we developed a rapid ATP bioluminescence-based method for detecting antibiotic resistance starting from positive blood culture. To minimize background ATP originating from blood corpuscles, specimens were centrifuged and the supernatant diluted with broth, and an ATP-eliminating reagent was then added to the bacterial suspension at the beginning of incubation. This newly devised procedure reduced the background ATP by more than five orders of magnitude. In a pilot study using levofloxacin, no false-susceptible results were observed in 15 clinical specimens. Furthermore, the results indicated that the rapid method provided additional information about bacterial activities with high resolution, in contrast to the less-thorough findings with the conventional turbidity method. Therefore, our approach will contribute to the treatment of infectious diseases as a rapid antimicrobial susceptibility test.

scholarly journals An Evaluation of the Rapid Antimicrobial Susceptibility Test by VITEK MS and VITEK 2 Systems in Blood Culture

2017 ◽  
Vol 49 (3) ◽  
pp. 279-284
Author(s):  
Kang-Gyun Park ◽  
Young-Bin Yu ◽  
Keundol Yook ◽  
Sang-Ha Kim ◽  
Sunghyun Kim ◽  
...  
Keyword(s):  
Blood Culture ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Test ◽  
Antimicrobial Susceptibility Test ◽  
Vitek 2 ◽  
Vitek Ms
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