The Role of Toll-Like Cell Receptor 2 (Arg753gln) Polymorphism in Allergen Sensitization and Disease Severity in Atopic Dermatitis Patients

2021 ◽  
Vol 30 (1) ◽  
pp. 29-37
Author(s):  
Nissreen E. ELBadawy ◽  
Nahed E Mostafa ◽  
Khaled Gharib
Keyword(s):  
Atopic Dermatitis ◽  
Disease Severity ◽  
Cell Receptor ◽  
Specific Ige ◽  
House Dust Mites ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Allergen Sensitization ◽  
Tlr2 Gene ◽  
Inhalant Allergens

Background: Variations in Toll-like receptor 2 (TLR2) encoding gene have been associated with atopic conditions. Objective: The present work aims to analyze single nucleotide polymorphism (SNP) of TLR2 gene Arg753Gln in atopic dermatitis (AD) and its association with allergen sensitization and disease severity. Methodology: 110 AD patients and 75 healthy controls were enrolled and subjected to genotyping of TLR2 gene Arg753Gln by restriction enzyme analysis and allergy investigations. Results: TLR2 Arg753Gln SNP was significantly more frequent in the patients (48%) in comparison to the healthy group (32%) (0R= 1.7). Individuals with the G/A genotype were at a higher risk for AD development by two times. Inhalant allergens specific IgE were distinguished in 80 % of patients with TLR2 gene polymorphism. Conclusion: GA genotype of TLR2 gene is more dominant in severe cases of atopic dermatitis and associated with sensitization to certain inhalant allergens as house dust mites and pollens.

scholarly journals Relations between vitamin D3, total and specific IgE for house dust mites in atopic dermatitis patients

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hassan M. Ibrahim ◽  
Moustafa A. El-Taieb ◽  
Mohammed H. Hassan ◽  
Abd Allah E. Mohamed ◽  
Ebtihal A. Kotop ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Negative Correlation ◽  
Vitamin D3 ◽  
House Dust ◽  
Specific Ige ◽  
Blood Analysis ◽  
House Dust Mites ◽  
Dust Mites ◽  
Cross Sectional ◽  
Inhalant Allergens

AbstractAtopic dermatitis (AD) is a chronic recurrent inflammatory skin disease. There are a lot of evidences on the importance of vitamin D and house dust mite (HDM) allergens in the etiology and course of AD. The objectives of this study are to evaluate the relation between vitamin D3 level and house dust mites (HDM) Dermatophagoidspecies sensitization in pathogenesis of atopic dermatitis. Cross-sectional design study was conducted on 50 atopic dermatitis patients. Blood analysis were done to determine level of vitamin D3, total IgE by fluorescent immunoassay & specific IgE for HDM (d1, d2) & other inhalant allergens by ELISA test. There was significant high negative correlation with the specific IgE for HDM (r =  −0. 62, p < 0.001) and vitamin D3. & there was non-significant minimal negative correlation with the specific IgE and other inhalant allergens (r = − 0.10, p > 0.05). There was a statistically significant relation between level of vitamin D3 and atopic dermatitis severity and sensitization to HDM and other allergens.

The sweat of patients with atopic dermatitis contains specific IgE antibodies to inhalant allergens

1996 ◽  
Vol 21 (5) ◽  
pp. 347-350 ◽  
Author(s):  
K. JUNG ◽  
G. SCHLENVOIGT ◽  
K. LADWIG ◽  
D. HERRMANN ◽  
C. MOTHS ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Specific Ige ◽  
Ige Antibodies ◽  
Specific Ige Antibodies ◽  
Inhalant Allergens

scholarly journals Atopic Dermatitis: Identification and Management of Complicating Factors

2020 ◽  
Vol 21 (8) ◽  
pp. 2671 ◽  
Author(s):  
Risa Tamagawa-Mineoka ◽  
Norito Katoh
Keyword(s):  
Atopic Dermatitis ◽  
Skin Barrier ◽  
Specific Ige ◽  
House Dust Mites ◽  
Patient Specific ◽  
Skin Barrier Function ◽  
Predictive Values ◽  
Prick Test ◽  
Complicating Factors ◽  
Microbial Organisms

Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease, associated with impaired skin barrier function and an atopic background. Various complicating factors, such as irritants, aeroallergens, food, microbial organisms, contact allergens, sweat, and scratching can induce the development of AD symptoms. Irritants, including soap/shampoo and clothes, can cause itching and eczematous lesions. In addition, young children with AD tend to become sensitized to eggs, milk, or peanuts, while older children and adults more often become sensitized to environmental allergens, such as house dust mites (HDM), animal dander, or pollen. Serum-specific IgE levels and skin prick test reactions to food tend to show high negative predictive values and low specificity and positive predictive values for diagnosing food allergy. On the other hand, AD adult patients tend to have severe skin symptoms and exhibit high HDM-specific IgE levels. Microbial organisms, e.g., Staphylococcus aureus and Malassezia furfur, might contribute to the pathogenetic mechanisms of AD. While sweat plays a major role in maintaining skin homeostasis, it can become an aggravating factor in patients with AD. Furthermore, scratching often exacerbates eczematous lesions. Several patient-specific complicating factors are seen in most cases. The identification and management of complicating factors are important for controlling AD.

scholarly journals Clinic al and immunologic al characteristic ofinfants with atopic dermatitis

2011 ◽  
Vol 8 (5) ◽  
pp. 31-36
Author(s):  
Natal'ya Aleksandrovna Ivanova ◽  
G A Kuz'mina ◽  
L T Kochish ◽  
N N Begaeva ◽  
N M Afanas'eva ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
House Dust ◽  
Clinical Manifestations ◽  
Milk Proteins ◽  
Goat Milk ◽  
Specific Ige ◽  
Cow Milk ◽  
Enzyme Analysis ◽  
Group I ◽  
Chicken Eggs

Background. The research is focused on the particularities of formation and course of atopic dermatitis in infancy depending on the range of sensitization and class of revealed antibodies. Methods. The article presents the results of clinical and immunological observation of 116 children aged from 1 month to 3 years with the clinical-anamnestic signs of atopic dermatitis. The immunological observation consisted of the following stages: determination of concentration of the general IgE, quantitative testing of concentrations of the serum IgE and IgG4 antibodies to allergens of cow and goat milk, chicken eggs and wheat, house dust and through the immune-enzyme analysis. Results and discussion. According to the results of the immunological observation the patients were divided into 3 groups: I - IgE+IgG4 - positive, II - IgG4 - positive, III - absence of above-mentioned classes of antibodies. The patients of group I are characterized by the early sensitization to cow milk proteins, the manifestation of the disease up to 3 months appears on pure breast feeding. The detection of the specific IgE antibodies to allergens of chicken eggs is a sign of danger of the development of sensitization towards inhalation house dust allergens and of the formation of respiratory allergy. For the children of group II the later age of manifestation of skin process (in more than half of children after introduction of milk food) and easier course of disease are significant. Anamnesis of the disease and the clinical manifestations of one third of patients of group III in the absence of the evidence of IgE-sensitization coincides with that of the patients of group I (age of manifestation, severity of disease). In accordance with received results, we can suggest that in this group there are patients with the unidentified immunopathological mechanism of formation of the disease. Conclusion. The immunological observation of infants by the detection of the specific IgE and IgG4 antibodies to widespread food allergens allows to reveal the peculiarities of immune response which in their turn define the clinical-anamnestic signs of atopic dermatitis.

Diagnostic specificity of the Fcε-receptor based allergy screening test

2009 ◽  
Vol 37 (04) ◽  
pp. 239-243
Author(s):  
R. Neiger ◽  
M. Linek ◽  
N. Thom
Keyword(s):  
Atopic Dermatitis ◽  
House Dust ◽  
Screening Test ◽  
Skin Diseases ◽  
Specific Ige ◽  
House Dust Mites ◽  
Dust Mites ◽  
Diagnostic Specificity ◽  
Allergen Specific Immunotherapy ◽  
Allergy Testing

Summary Objective: To evaluate the diagnostic specificity of a Fcε-receptor allergy screening test using serum from clinically non-atopic dogs. Material and methods: In this prospective study allergen-specific IgE levels were measured using the Fcε-receptor method (Allercept® Heska) in the serum from 15 clinically non-atopic dogs, without any history of pruritic skin diseases or recurrent ear diseases. Five crossbreeds and 10 pure breed dogs were included with approximately equal sex distribution. Their age ranged between 2 and 14 years, with a median age of 4 years. A commercially available allergy screening test was performed evaluating three allergen groups: mites (house dust mites and forage mites), moulds and pollens, as well as flea-saliva. Results: All except one dog showed positive results for at least one of the allergen groups. Fourteen dogs were positive for mites (house dust mites and forage mites), five dogs each were additionally positive for flea-saliva antigen and for pollens, respectively; only one dog was positive for moulds. Overall diagnostic specificity of the allergy screening test was 6.7% for mites, 66.6% for flea-saliva and pollens and 93.3% for moulds. Conclusions: The Fcε-receptor based allergy screening test detects a high incidence of allergen-specific IgE among clinically non-atopic dogs. The low diagnostic specificity in this study confirms results of earlier studies with serological monoclonal and polyclonal allergy testing. Clinical relevance: This Fcε-receptor based allergy screening test should not be used to establish the diagnosis of canine atopic dermatitis. Diagnosis of atopic dermatitis in dogs is based on clinical criteria and exclusion of other causes of pruritus. Positive intradermal or serological allergy testing is only a minor diagnostic criterion. Any kind of allergy testing is performed for identification of the relevant allergens in atopic dogs as a precondition for allergen specific immunotherapy.

The sweat of patients with atopic dermatitis contains specific IgE antibodies to inhalant allergens

1996 ◽  
Vol 21 (5) ◽  
pp. 347-350 ◽  
Author(s):  
K. JUNG ◽  
G. SCHLENVOIGT ◽  
K. LADWIG ◽  
D. HERRMANN ◽  
C. MOTHS ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Specific Ige ◽  
Ige Antibodies ◽  
Specific Ige Antibodies ◽  
Inhalant Allergens

scholarly journals Atopic dermatitis in children and specific IgE to Stahpylococcus aureus superantigens: a compare with disease severity

2012 ◽  
Vol 9 (4) ◽  
pp. 39-41
Author(s):  
M S Treneva ◽  
A N Pampura ◽  
T S Okuneva
Keyword(s):  
Atopic Dermatitis ◽  
Disease Severity ◽  
Specific Ige

Background. Staphylococus aureus (S. aureus ) function in atopic dermatitis (AD) children skin is double-handed: an infectious trigger and an allergen. The study was aimed to evaluate a frequency of S. aureus enterotoxins IgE work out in children with infected AD skin and to compare IgE levels with infected AD severity. Methods. Specific IgE to S. aureus enterotoxins A and B (ImmunoCap, Phadia AB, Sweden) detected in serum of 90 children with infected AD. IgE levels were compared to severity of infected AD (Mann—Whitney Utest). Results. Specific IgE>0,35 kUA/l to S. aureus enterotoxin A frequency was of 0,29; to enteritoxin B 0,36; to one of them 0,43. IgE to S. aureus enterotoxins were elevated in 16 children with therapy resistant AD in compare to other cases of AD (р=0,00183). Conclusion. In children with infected AD S. aureus enterotoxins A or/and B are revealed in 25—50% of cases. In children with therapy resistant AD specific IgE level to enterotoxins is elevated in comparison to severe or average AD children.

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