A Bacteriological study on mineral trioxide aggregate and calcium hydroxide as materials against Streptococcus mutans

2021 ◽  
Vol 30 (1) ◽  
pp. 1-7
Author(s):  
Kareman A. Eshra ◽  
Rasha Adel Elkholy ◽  
Arafa Mohamed Khatab ◽  
Radwa Abd Elmotaleb Eissa

Background: Streptococcus mutans play an important role in occurrence of dental caries objective: to compare the clinical and microbiological antibacterial outcomes of Mineral trioxide aggregate (MTA) and Calcium hydroxide cement Ca(OH)2. Methodology: 20 primary molars in 10 children with year’s 5-9 y .For each child one tooth was treated with Ca(OH)2 and the other with MTA. Finally, all the cavities were restored using compomer restorative material, and then microbiological parameters were recorded. Results: MTA treated teeth did not show any clinical sign or symptoms of failure. While three teeth treated with Ca(OH)2 were excluded because of necrosis. Changes in color and consistency of dentin were nearly the same for both groups. Microbiological evaluation showed a decrease in the count of Str. mutans with calcium hydroxide and complete killing of bacteria with MTA. Conclusion: The treatment showed satisfactory results of MTA as it was more potent inhibitor of bacterial-growth than Ca(OH)2

2021 ◽  
Vol 9 (4) ◽  
pp. 764-768
Author(s):  
Vigneshwari S.K ◽  
◽  
Manje Gowda ◽  
Jayalakshmi K.B ◽  
Dhanavel. C ◽  
...  

Aim: To evaluate and compare the antibacterial efficacy of Cention-N in two different curing methods. Methods: Twenty blocks of Cention-N were prepared according to standard manufacturers instructions and were divided into two groups-self cured and light cured (ten blocks in each group). Streptococcus mutans were extracted from dental caries by a series of biochemical treatments and strains of S.mutans were treated with both groups of Cention-N blocks. Results: Self cured Cention-N blocks exhibited statistically significant reduction in bacterial colonies compared to Light-cured blocks. Conclusion: Additional light curing inhibits the antibacterial efficacy of Cention-N cement.


2020 ◽  
pp. 01-07
Author(s):  
KITAJIMA Kayoko ◽  
ARAI Kyoko ◽  
YOKOSUKA Takashi ◽  
SATOH Tomonori ◽  
KITANO Yoshie ◽  
...  

Root-end restoration using Mineral Trioxide Aggregate (MTA) was performed on two patients who received root canal treatment but had poor progress. In one case, a large amount of Calcium hydroxide(Ca (OH)2), a intracanal medicament, escaped from a wide-open apex, stagnated around the tooth root, and the apical lesion did not disappear. The other case is a case where the root canal at the apical part has a complicated anatomical form, the root canal cannot be tightly packed, and the apical lesion has not disappeared. Root-end restoration using MTA was performed on these two cases, and improvement of the lesion was confirmed. From these results, it was shown that Root-end restoration using MTA is effective for patients with poor prognosis of root canal treatment for which the apical portion cannot be tightly packed for various reasons. Keywords: Mineral Trioxide Aggregate; MTA; Root-end restoration


2014 ◽  
Vol 3 (1) ◽  
pp. 24-31
Author(s):  
Md Farid Uddin ◽  
Md Shamsul Alam ◽  
Ali Asgor Moral ◽  
Md Mujibur Rahman Howlader ◽  
Md Ismail Hossain

Background: The greatest threats to developing teeth are dental caries and traumatic injury. If pulpal exposure occurs, then a pulpotomy procedure aims to preserve pulp vitality to allow for normal root development. Historically, calcium hydroxide has been the material of choice for pulpotomy procedures. Recently, an alternative material called mineral trioxide aggregate (MTA) has demonstrated the ability to induce hard-tissue formation in pulpal tissue. Objectives: This prospective study was conducted to observe the clinical and radiological findings of pulpotomies done with Mineral Trioxide Aggregate (MTA) &Calcium Hydroxide{Ca(OH)2} and to evaluate success rate of MTA in maintaining pulpal health in teeth with carious lesion. Materials and Methods: In this clinical trial study, 40 primary molars were treated by a conventional pulpotomy technique. They all fulfill the inclusion criteria and gave consent regarding this study. All selected teeth were evenly divided into 2 test groups. In group 1, the MTA pulpotomy (experimental) was performed, whereas in group 2, the conventional Ca(OH)2 pulpotomy (control) was done. The teeth of both groups were finally restored by Glass Ionomer Cement (GIC). The children were recalled for clinical and radiographic evaluations after 3, 6, and 12 months. Result: The follow-up evaluations revealed failure due to pain and swelling detected at 6 and 12 months postoperative evaluations in only 3 teeth treated with Ca(OH)2 and one treated with MTA. The remaining 36 teeth appeared to be clinically and radiographically successful 12 months postoperatively. Calcific metamorphosis was a radiographic finding in 4 teeth treated with MTA and 2 teeth treated with Ca(OH)2. Conclusion: Based on this clinical and radiographical evaluation study of 3, 6, and 12 months follow-up, MTA could be used as a safe material for pulpotomy in cariously and mechanically exposed primary molars and seems to be a suitable alternative to calcium hydroxide. Further research, however, is required to clarify this conclusion. DOI: http://dx.doi.org/10.3329/updcj.v3i1.17981 Update Dent. Coll. j: 2013; 3 (1): 24-31


2014 ◽  
Vol 08 (02) ◽  
pp. 234-240 ◽  
Author(s):  
Esma Yildiz ◽  
Gul Tosun

ABSTRACT Objective: The aim of this study is to evaluate four different pulpotomy medicaments in primary molars. Materials and Methods: A total of 147 primary molars with deep caries were treated with four different pulpotomy medicaments (FC: formocresol, FS: ferric sulfate, CH: calcium hydroxide, and MTA: mineral trioxide aggregate) in this study. The criteria for tooth selection for inclusion were no clinical and radiographic evidence of pulp pathology. During 30 months of follow-up at 6-month intervals, clinical and radiographic success and failures were recorded. The differences between the groups were statistically analyzed using the Chi-square test and Kaplan-Meier analysis. Results: At 30 months, clinical success rates were 100%, 95.2%, 96.4%, and 85% in the FC, FS, MTA, and CH groups, respectively. In radiographic analysis, the MTA group had the highest (96.4%), and the CH group had the lowest success rate (85%). There were no clinical and radiographic differences between materials (P > 0.05). Conclusions: Although there were no differences between materials, only in the CH group did three teeth require extraction due to further clinical symptoms of radiographic failures during the 30-month follow-up period. None of the failed teeth in the other groups required extraction during the 30-month follow-up period.


Author(s):  
M. J. Kramer ◽  
Alan L. Coykendall

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.


2019 ◽  
Vol 14 (1) ◽  
pp. 92
Author(s):  
Dr. Maha Abdul- Kareem Mahmood ◽  
Dr. Huda Elias Ali ◽  
Dr. Haraa Khairi Abdul-Kadher

Microbes are considered as the primary etiologic agents in endodontic diseases.Disinfection of the root canal is obtained by the combined effect of biomechanicalpreparation, irrigation and intra canal medicament. The aim of the present study wasto assess the antimicrobial activity of intracanal medicaments (formocresol andEndosepton) against two micro organisms (Streptococcus mutans and staphylococcusaureus) isolated from 15 necrotic pulps of primary molars indicated for pulpectomyprocedure. The samples were cultured, and purified using microbiological evaluation.Broth dilution test was performed in our study by preparing test tubes containing10 ml of BHI broth (pH. 7) which then inoculated with strains of the tested bacteriaand incubated at 37 C° for 24 h. After over night incubaction, ten fold dilution weremade in test tubes containing 9 ml of normal saline by adding 1 ml of the inoculum tothe first tube . Then from dilution 10-1 , 0.1 ml of cell suspension was added to 9.9 mlof formocresol and endosepton, then 0.1 ml was taken and spread on duplicates ofBHI agar plates at different intervals and incubated aerobically for 24 h. at 37 C°.Colonies on the plates were counted after incubation and CFU/mL (colony formingunit) was calculated. Our results indicating that there were no significant differencesbetween the intracanal medicaments, but there were high significant differencesbetween the intervals time of the study. We concluded that both materials had greatantibacterial effect against the pathogens commonly isolated from necrotic pulpaltissue of primary teeth.


2017 ◽  
Vol 68 (2) ◽  
pp. 323-327
Author(s):  
Cristian Levente Giuroiu ◽  
Maria Vataman ◽  
Gabriel Melian ◽  
Dragos Bularda ◽  
Ludmila Lozneanu ◽  
...  

The study aimed to assess the number, localization and distribution of interleukin 6 (IL-6) positive cells in healthy pulp, acute and chronic pulpitis. The study group included 48 patients aged between 18-72, treated in University of Medicine and Pharmacy Grigore T. Popa Iasi, Romania. The pulpectomy was performed on 42 patients diagnosed with acute and chronic pulpitis. The other 6 patients, without signs of dental caries or periodontal disease, were submitted to extractions of teeth for orthodontic purposes, with pulpectomy performed before extraction. The pulp samples were examined with optic microscope. The detection and assessment of IL-6 were performed using immunohistochemical technique. Data were statistically analysed using non-parametric tests. According to morphopathological criteria, 42.85% were classified as acute pulpitis and 57.14% as chronic pulpitis. The pulp samples in control group were not associated with IL-6 positive cells. The analysis of all samples with acute and chronic pulpitis identified 73.80% samples with IL-6 and 26.20% associated with the absence of IL-6. The highest frequency of IL-6 positive cells was recorded in rich-cell zone of crown dental pulp. The systemic distribution of IL-6 positive cells was mostly diffused without well-defined orientation. IL-6 release in acute and chronic pulpitis is significantly higher comparing with healthy pulp tissue.


Sign in / Sign up

Export Citation Format

Share Document