scholarly journals A controlled in vitro study of the antimicrobial effectiveness of Colibacillinum against E. coli

2018 ◽  
Author(s):  
◽  
Nkululeko Xaba
Keyword(s):  
In Vitro Study ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Disc Diffusion ◽  
Antimicrobial Effects ◽  
E Coli ◽  
Enteropathogenic Strain ◽  
Antimicrobial Effectiveness

Background Due to the rise of antibiotic resistant bacterial infections, alternative methods of treatment need to be explored. Homoeopathic medicine is based on the principle of ‘like cures like’ (O’Reilly, 1996) i.e. the symptoms the substance can cause, it can cure which is the underlying rationale for this study. Colibacillinum is a remedy prepared from an enteropathogenic strain of E. coli, this remedy is already used in clinical practice against chronic cystitis (Leupen, 2010) caused by E. coli, however, an extensive literature search has revealed there to be no empirical investigation into its antibiotic capacity. This study aimed to show whether or not this remedy demonstrates antimicrobial capacity against E. coli in an in vitro setting. Parallell homoeopathic potencies of this remedy and one prepared from a uropathogenic strain of E. coli were tested for antimicrobial effects against enteropathogenic and uropathogenic strains of E. coli in vitro using the disc diffusion method. Aim of the study The aim of this controlled in vitro study is to determine the antimicrobial effectiveness of parallel potencies of the homoeopathic remedy Colibacillinum (manufactured from a uropathogenic strain and enteropathogenic strains respectively) against uropathogenic and enteropathogenic cultures of E. coli in vitro by means of the disc diffusion assay method. Methodology Measurements were by means of the disc diffusion essay. For this experiment thirty Mueller-Hinton plates were prepared and inoculated with each test bacteria in turn. Fifteen plates were inoculated with Uropathogenic strain of E. coli and the remaining 15 plates were inoculated with Uropathogenic strain of E. coli. A sterile 5mm Whatman® filter paper number 4 discs were individually inoculated with test substances 3CH,9CH,30CH and 200CH potencies and the controls, negative (43% ethanol) and positive control (Ciprofloxacin) using a micropipette, before being allowed to dry in the incubator. A Ciprofloxacin antibiotic (positive control) was included in the experiment with sole purpose of accounting for plate-plate variations in the pharmacological sensitivity of the same specie of bacteria. The plates were incubated at 37°C, and the zones of inhibition measured with a pair of Vernier calipers at 24 hours. Data entry was done using the SPSS statistical package. ANOVA was used to compare the differences between the test and control groups, Mauchly’s Test of Sphericity for Uropathogenic prepared strain, Mauchly’s Test of Sphericity for Enteropathogenic prepared strain, Normality test. Results The results obtained from this study showed that the Homoeopathic remedy Colibacillinum prepared from both Uropathogenic and Enteropathogenic strains displayed inhibitory effects against Enteropathogenic and Uropathogenic strains of E. coli, and exhibited statistically significance. The control group (ciprofloxacin) had the highest inhibitory effect (42.3±0.58mm) against Enteropathogenic and Uropathogenic E. coli, while the negative control (43% ethanol) had the lowest inhibitory effect (0.67±1.15mm). Colibacillinun 200CH prepared from a Uropathogenic strain of E-coli (Coli-b_U 200CH) displayed statistically significant antimicrobial effects against uropathogenic E.coli; such antimicrobial effects were significantly greater than 43% ethanol (negative control); the antimicrobial effect was however inferior to Ciprofloxacin (positive control). Colibacillinum 9CH prepared from Enteropathogenic strain of E-coli (Coli-b_E 9CH) also displayed statistically significant antimicrobial effects against enteropathogenic E.coli which were significantly greater than 43% ethanol (negative control) but inferior to Ciprofloxacin. Conclusion This study concluded that Colibacillinum prepared from Uropathogenic and Enteropathogenic strains of E. coli, are effective in inhibiting the in vitro growth of E.coli when evaluated by means of disc diffusion. The study further confirmed that the biological (anti-microbial) activity of an ultra-high homoeopathic dilution (Coli-b_U 200CH) (1:10400) and in the case of Coli-b_U the findings support existing literature which suggests that the anti-microbial properties of homeopathic nosodes increase with potency; all hypotheses for this remedy were thus accepted. This trend was not noted for Coli-b_E in which the potency with the greatest anti-microbial effect was the 9CH, thus Colibacillinum prepared from Enteropathogenic strain (Coli-b_E) did not conform with hypothesies one, two and four that were proposed in chapter one. Despite this the confirmation of significant antimicrobial effects of a substance at this level of deconcentration (1:1018) is noteworthy.

scholarly journals Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

2016 ◽  
Vol 45 (4) ◽  
pp. 234-239 ◽  
Author(s):  
Priscilla Barbosa Ferreira SOARES ◽  
Aletheia Moraes ROCHA ◽  
Manuella Verdinelli de Paula REIS ◽  
Camilla Christian Gomes MOURA ◽  
Carlos José SOARES
Keyword(s):  
Cell Viability ◽  
Tap Water ◽  
Human Fibroblasts ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Coconut Water ◽  
Ph Adjustment ◽  
Adjustment Method

Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.

Comparative Evaluation of the Antimicrobial Effects of Different Mouthrinses against Streptococcus Mutans: An in Vitro Study

2019 ◽  
Vol 43 (6) ◽  
pp. 398-407
Author(s):  
Eman Abu-obaid ◽  
Fouad Salama ◽  
Ala’a Abu-obaid ◽  
Fars Alanazi ◽  
Mounir Salem ◽  
...  
Keyword(s):  
Reference Strain ◽  
In Vitro Study ◽  
Negative Control ◽  
Minimal Bactericidal Concentration ◽  
Distilled Water ◽  
Chlorhexidine Digluconate ◽  
Zone Of Inhibition ◽  
Antimicrobial Effects ◽  
Zones Of Inhibition

Purpose: To assess the antimicrobial effects of different natural and semi-natural mouthrinses on isolates of S. mutans obtained from the saliva of Saudi children and reference strains of S mutans (ATCC 25175). Study design: Saliva samples were collected from 20 children. Natural and semi-natural mouthrinses included were herbal mix mouthrinse, cranberry mouthrinse, chlorhexidine digluconate mouthrinse, cranberry extract mixed with chlorhexidine digluconate mouthrinse, chlorhexidine digluconate mouthrinse with alcohol (positive control), and distilled water (negative control). The microbiological examination tests were minimal inhibitory concentration, minimal bactericidal concentration, and zone of inhibition for the saliva isolates of S. mutans while zone of inhibition test only for reference strain of S. mutans. Results: For reference strain in a comparison with the distilled water, the herbal mix, cranberry, cranberry mixed with chlorhexidine, chlorhexidine, and chlorhexidine with alcohol showed significantly increased zones of inhibition by 36.38, 36.25, 26.13, 17.75, and 12.38, respectively. For saliva isolates in a comparison with the distilled water, the herbal mix, cranberry, cranberry mixed with chlorhexidine, chlorhexidine, and chlorhexidine with alcohol showed significantly increased zones of inhibition by 38.00, 34.25, 22.94, 16.50, and 16.44, respectively. Chlorhexidine with alcohol showed significantly lower minimum inhibitory and bactericidal concentration than the other groups. Conclusions: Herbal mix and cranberry mouthrinses could be effective natural alternative to chlorhexidine mouthrinse with or without alcohol in affecting tested parameters.

Evaluating Dentin Surface Treatments for Resin-Modified Glass Ionomer Restorative Materials

2013 ◽  
Vol 38 (4) ◽  
pp. 429-438 ◽  
Author(s):  
TA Imbery ◽  
A Namboodiri ◽  
A Duncan ◽  
R Amos ◽  
AM Best ◽  
...  
Keyword(s):  
Testing Machine ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Surface Treatments ◽  
Band Matrices ◽  
Dentin Surface ◽  
Occlusal Surfaces ◽  
Significant Difference

SUMMARY This in vitro study evaluated the effect of six surface treatments on the shear bond strength of three resin-modified glass ionomers (RMGIs) to dentin. Occlusal surfaces of caries-free third molars were reduced to expose only dentin. Surface treatments were smear layer intact (negative control), Cavity Conditioner, EDTA, Ketac Primer, Self Conditioner, and etching with 35% phosphoric acid followed by the application of Optibond Solo Plus. Filtek Z250 composite resin bonded with Optibond Solo Plus served as a positive control. Conditioning agents were used according to the manufacturers' instructions. After surface treatments, Fuji II LC, Riva LC, Ketac Nano, and Filtek Z250 were placed in copper-band matrices 5 mm in diameter and 2 mm in height and were light-cured for 20 seconds. Specimens were stored in 100% humidity for 24 hours, after which they were placed in deionized water for 24 hours at 37°C. They were then tested under shear forces in an Instron Universal Testing Machine at a crosshead speed of 0.5 mm/min. A two-way analysis of variance and Tukey honestly significant difference statistical analyses (p<0.05) indicated significant interaction between RMGIs and conditioning agents. Acid etching followed by Optibond Solo Plus provided highest bond strengths for all three RMGIs, which were not statistically different from the positive control.

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

2017 ◽  
Vol 40 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Faika Y. Abdelmegid ◽  
Fouad S. Salama ◽  
Waleed M. Al-Mutairi ◽  
Saud K. Al-Mutairi ◽  
Sultan O. Baghazal
Keyword(s):  
Primary Teeth ◽  
In Vitro Study ◽  
Class Ii ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

scholarly journals Microbiological Sealing Analysis of a Tapered Connection and External Hexagon System

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Gardel Nepomuceno Costa ◽  
Elizabeth Ferreira Martinez ◽  
Aluísio Martins de Oliveira Ruellas ◽  
Daiane Cristina Peruzzo ◽  
Júlio Cesar Joly ◽  
...  
Keyword(s):  
Descriptive Analysis ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Microbiological Analysis ◽  
Significance Level ◽  
Implant Abutment ◽  
Bacterial Leakage ◽  
Tapered Connection

Considering the variety of implant connection systems available in the market and the contrasting literature regarding tapered connection systems in terms of bacterial leakage, the aim of this in vitro study was to compare the effectiveness of the bacterial seal at the implant/abutment interface between an external hexagon and a tapered connection system. Twelve sets of indexed tapered connection components and twelve sets of external hexagon connection components were used for microbiological analysis. In addition, for each model, an implant with its respective prosthetic abutment was used as a negative control and another as a positive control of microbial contamination. Failure of the abutment/implant interface seal was observed via turbidity or presence of deposits in the culture. Descriptive analysis of the data and relative frequency (percentage) as well as Fisher’s exact test were used at a significance level of 5%. Two of ten (20%) external hexagon specimens showed contamination against 0/10 (0%) tapered connection implants. In conclusion, both implant/abutment connections were able to prevent bacterial leakage in vitro.

scholarly journals In vitro Evaluationof the Antimi- crobial Activity of a Topical Skin Preparation Containing 0.1% Polyhexanide vs a Topical Skin Prepa-ration Containing 1% Silver Sulfadiazine

2021 ◽  
Vol 6 (2) ◽  
pp. 1-7
Author(s):  
Barbara Maglione ◽  
Keyword(s):  
Antibacterial Activity ◽  
Positive Control ◽  
Negative Control ◽  
Silver Sulfadiazine ◽  
Bacterial Strains ◽  
Skin Preparation ◽  
E Coli ◽  
Topical Cream ◽  
Significant Difference

Aim: The effective in vitro antibacterial activity on Staphylococcus aureus (S.aureus), Pseudomonas aeruginosa (P.aeruginosa), Klebsiella pneumoniae (K.pneumoniae),Escherichia coli (E.Coli) and the combination of S.aureus and K. pneumonia of a topical cream based on 0.1% polyhexanidewas compared to a topical cream based on 1% silver sulfadiazine.A topical cream containing 0,1% gentamicin was used as a positive control and a white blank topical cream was used as negative control. Materials and Methods: The in vitro antibacterial activities were determined by agar well-diffusion assay. Two-way Analysis of Variance (ANOVA) was used to test, by calculation of P-values, for significant antiseptic activity in bacteria treated with 0.1% polyhexanide topical cream compared to 1% silver sulfadiazine and to the negative and positive controls. Results: Among the derivatives tested, all the active topical creams analyzed were able to reduce microbial strains. The topical cream based on 0.1% polyhexanide showed a significantly higher antibacterial efficacy in comparison to the topical cream based on 1% silver sulfadiazine on S. aureus and K. pneumonia and on the combination of S. aureus and K. pneumoniae,while no significant difference was detected between the antibacterial activity of the two topical creams against P. aeruginosa and E. coli. Conclusion: These results provide a further insight into the antibacterial activity of polyhexanide and its non-inferiority compared to silver sulfadiazine towards certain bacterial strains (P. aeruginosa and E. coli) and superiority towards other (S. aureus and K. pneumoniae)and support the use of 0.1% Polyhexanide topical preparation for the treatment of wounds that are infected or at risk of infection.

scholarly journals In vitro antimicrobial evaluation of toothpastes with natural compounds

2015 ◽  
Vol 09 (04) ◽  
pp. 580-586 ◽  
Author(s):  
Priscila de Camargo Smolarek ◽  
Luis Antonio Esmerino ◽  
Ana Cláudia Chibinski ◽  
Marcelo Carlos Bortoluzzi ◽  
Elizabete Brasil dos Santos ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
In Vitro Study ◽  
Study Groups ◽  
Natural Compounds ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method

ABSTRACT Objectives: This in vitro study evaluated the antimicrobial effects of commercial toothpastes containing natural compounds. Materials and Methods: The study groups were divided based on the natural compound present in the toothpaste composition: Sorbitol (I), tocopherol (II), mint (III), cinnamon/mint (IV), propolis/melaleuca (V), mint/açai (VI), mint/guarana (VII), propolis (VIII), negative control (IX), and the positive control (X). The antimicrobial properties of the toothpastes were tested using the disk diffusion method against oral pathogens: Streptococcus mutans, Pseudomonas aeruginosa, and Enterococcus faecalis. The resulting inhibition halos were measured in millimeters. Results: The data indicated that the bacteria responded differently to the toothpastes (P < 0.0001). The diameters of the inhibition halos against S. mutans were in decreasing order of efficacy: Propolis/melaleuca > mint/guarana > mint/açai > sorbitol > tocopherol > cinnamon/mint > propolis > mint (P < 0.001 vs. negative control). E. faecalis showed variable responses to the dentifrices in the following order of decreasing efficacy: Mint/guarana > propolis > sorbitol > mint/açai > tocopherol > cinnamon/mint > mint = propolis/melaleuca = negative control. The product with the highest antimicrobial activity was mint/guarana, which was significantly different than propolis/melaleuca, mint, cinnamon/mint, and tocopherol and negative control (P < 0.001). The statistical analysis indicated that propolis, sorbitol, and mint/açai did not show any differences compared to mint/guarana (P > 0.05) and positive control (P > 0.05). P. aeruginosa was resistant to all dental gels tested including positive control. Conclusion: The toothpastes with natural compounds have therapeutic potential and need more detailed searches for the correct clinic therapeutic application. The results from this study revealed differences in the antimicrobial activities of commercial toothpastes with natural compounds.

In Vitro Study on Protoscolicidal Effect of Methanolic Extract of Allium hirtifolium on Protoscoleces of Cystic Echinococcosis

2019 ◽  
Vol 19 (3) ◽  
pp. 264-268
Author(s):  
Z. Shahamir Tabatabaei ◽  
S. Dehshahri ◽  
M.M. Taghi ◽  
F. Esfandiari ◽  
F.S. Sadjjadi ◽  
...  
Keyword(s):  
Cystic Echinococcosis ◽  
Allium Sativum ◽  
Methanolic Extract ◽  
In Vitro Study ◽  
Cyst Formation ◽  
Positive Control ◽  
Negative Control ◽  
Cyst Rupture ◽  
Allium Hirtifolium

Background: Cystic echinococcosis/ hydatidosis is one of the most important parasitic zoonoses worldwide. Usually, surgery is used for the treatment of active cysts. Due to the risk of cyst rupture during surgery, a protoscolicidal agent is usually injected into the cyst before surgery to prevent secondary cyst formation in case of cyst rupture. Due to the side effects of chemical protoscolicidal agents attempts have been focused on herbal medicine. In this regard, Allium sativum has already been used; however, the protoscolicidal effect of Allium hirtifolium from Allium family has not been investigated, so far. Objective: The present study was conducted in order to assess the protoscolicidal effect of methanolic extract of Allium hirtifolium in different concentrations, temperature and exposure times. Cetrimide as a positive control and DMSO as a negative control were also used in this study. Methods: The viability of protoscoleces was determined by 0.1% eosin staining method. Results: The protoscolicidal effect of Allium hirtifolium on protoscoleces was as follows: The mean percent and standard deviation (SD) of protoscolicidal activity of 50 mg/ml concentration of Allium hirtifolium extract in room temperature at 10, 20, 30, 40 and 50 minutes of exposure was 5.88±2.39, 8.59±4.54, 11.82±5.81, 12.31±6.16and 16.96±8.42 respectively; while at 37°C it was 9.10±5.01, 11.52±7.36, 18.64±4.36, 27.07±6.57 and 38.15±12.80, respectively. Conclusion: In conclusion, although Allium hirtifolium from Allium family is similar to Allium sativum; the protoscolicidal effect of its methanolic extract is not similar and less than A. sativum extract.

scholarly journals SKRINING FITOKIMIA DAN AKTIVITAS ANTIBAKTERI in vitro EKSTRAK HEKSANA-PETROLEUM ETER Artemisia cina Berg. ex Poljakov

Agric ◽  
2016 ◽  
Vol 27 (1) ◽  
pp. 30
Author(s):  
Elizabeth B.E Kristiani ◽  
Sri Kasmiyati ◽  
Maria Marina Herawati
Keyword(s):  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
E Coli ◽  
Randomized Design ◽  
Antibactrial Activity

<p>The objectives of this reserch were to determine the antibacterial activity of hexane-petroleum ether (1 : 1 v/v) extract of Artemisia cinaBerg. ex Poljakov on Escherichia coli and Staphylococcus aureus and its compounds. The design of the research was using completely Randomized Design in five concentration of extract that were 0, 50, 75, 100, 150, dan 200 mg/ml with four repliceted. Ethanol 60% was used as negative control and tetracycline as positive control. There were three steps of research. First step was extracted the plant using soxhlet method with hexane-petroleum ether (1:1 v/v). Second step was determined the antibacterial activity of hexane-petroleum ether (1:1 v/v) extract in various concentration of that extracts on E. Coli and S. aureus using agar diffusion method. Analysis of Variance (ANOVA) and was used to determined the significan different of diameter of inhibition between the treatments. Thirdsteps was phytochemical analysis of extract. The highest antibactrial activity on E. Coli was at 100 mg/ml whereas on S. Aureus at 150 mg/ml. That extract was contained flavonoid, alkaloid, essential oils, saponin, sterol, tritepene, hydrolized tannin, and coumarin.</p>

scholarly journals In vitro evaluation of the antimicrobial activity of a topical skin preparation containing 0.1% polyhexanide vs a topical skin preparation containing 1% silver sulfadiazine

2020 ◽  
Author(s):  
G. Schiavo ◽  
D. Falciglia ◽  
S. Maurelli ◽  
S. Riccio ◽  
Barbara Maglione
Keyword(s):  
Antibacterial Activity ◽  
Positive Control ◽  
Negative Control ◽  
Silver Sulfadiazine ◽  
Bacterial Strains ◽  
Skin Preparation ◽  
E Coli ◽  
Topical Cream ◽  
Significant Difference

Abstract 1.1 Aim: The effective in vitro antibacterial activity on Staphylococcus aureus (S.aureus), Pseudomonas aeruginosa (P.aeruginosa), Klebsiella pneumoniae (K.pneumoniae), Escherichia coli (E.Coli) and the combination of S.aureus and K. pneumoniae of a topical cream based on 0.1% polyhexanide was compared to a topical cream based on 1% silver sulfadiazine. A topical cream containing 0,1% gentamicin was used as a positive control and a white blank topical cream was used as negative control.1.2 Materials and Methods: The in vitro antibacterial activities were determined by agar well-diffusion assay. Two-way analysis of variance (ANOVA) was used to test, by calculation of P-values, for significant antiseptic activity in bacteria treated with 0,1% polyhexanide topical cream compared to 1% silver sulfadiazine and to the negative and positive controls. 1.3 Results: Among the derivatives tested, all the active topical creams analyzed were able to reduce microbial strains. The topical cream based on 0.1% polyhexanide showed a significantly higher antibacterial efficacy in comparison to the topical cream based on 1% silver sulfadiazine on S. aureus and K. pneumoniae and on the combination of S. aureus and K. pneumoniae, while no significant difference was detected between the antibacterial activity of the two topical creams against P. aeruginosa and E. coli. 1.4 Conclusion: These results provide a further insight into the antibacterial activity of polyhexanide and its non-inferiority compared to silver sulfadiazine towards certain bacterial strains (P. aeruginosa and E. coli) and superiority towards other (S. aureus and K. pneumoniae) and support the use of 0.1% Polyhexanide topical preparation for the treatment of wounds that are infected or at risk of infection.

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