scholarly journals Antioxidant and antimicrobial activities of methanol leaf extract of Aegle Tamilnadensis Abdul Kader

2017 ◽  
Vol 9 (4) ◽  
pp. 648
Author(s):  
M Chella Kumar ◽  
S Abdul Kader ◽  
C Sivaraj ◽  
N Raaman
Keyword(s):  
Free Radical ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Diffusion Method ◽  
Antioxidant And Antimicrobial Activities ◽  
Radical Scavenging Assay

The present investigation was aimed to evaluate the phytochemical constituents, free radical scavenging activities, antioxidant properties and antimicrobial activities of the methanol leaf extract of <em>Aegle tamilnadensis </em>Abdul Kader. The phytochemicals present in the methanol leaf extract of <em>A. tamilnadensis</em> Abdul Kader<em> </em>were determined qualitatively and quantitatively using standard procedures. The antioxidant activities were carried out by DPPH free radical scavenging assay, OH<sup>•</sup> radical scavenging assay, NO<sup>• </sup>radical scavenging assay, Fe<sup>3+ </sup>reducing power assay, and phosphomolybdenum reduction assay methods. The antimicrobial activity was carried out by well diffusion method. The methanol leaf extract of <em>A. tamilnadensis</em> Abdul Kader<em> </em>showed good free radical scavenging as well as reducing power activities which were found to increase with the increasing concentration of the extract. The study revealed the presence of major phytochemicals such as phenols and flavonoids at the concentration of 211.0 mg/g at 100 µg and 52.91 mg/g at 100µg/mL respectively. The present study revealed that the methanol leaf extract of <em>A. tamilnadensis</em> Abdul Kader<em> </em>possess significant antioxidant and antimicrobial activities.

Heterogeneous Method for Grafting of Syringaldehyde onto Chitosan by Laccase Oxidation

2013 ◽  
Vol 477-478 ◽  
pp. 1340-1344
Author(s):  
Cheng Hua Yu ◽  
Ji Cheng Pei ◽  
Fang Dong Zhang
Keyword(s):  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Amino Groups

Heterogeneous method for grafting of syringaldehyde (SD) onto chitosan by laccase oxidation, yielding a product with improved antioxidant activities. The results showed that about 1.1% amino groups participated in this reaction. The FTIR showed that the absorption of amino groups were decreased, the absorption of benzene ring structures were disappeared and a new band appeared at 1637 cm-1 corresponding to C=N vibrations that were due to the Schiff base reaction between the chitosan and syringaldehyde. Additionally, the chitosan had insignificant reducing power and DPPH free radical scavenging activity, SD-chitosan had reducing power of A700=0.684, DPPH free radical scavenging activity of 53.2%. SD-chitosan presented improved antioxidant properties.

scholarly journals Mechanism of Action against Food Spoilage Yeasts and Bioactivity of Tasmannia lanceolata, Backhousia citriodora and Syzygium anisatum Plant Solvent Extracts

Foods ◽  
2018 ◽  
Vol 7 (11) ◽  
pp. 179 ◽  
Author(s):  
Fahad Alderees ◽  
Ram Mereddy ◽  
Dennis Webber ◽  
Nilesh Nirmal ◽  
Yasmina Sultanbawa
Keyword(s):  
Escherichia Coli ◽  
Bioactive Compounds ◽  
Mechanism Of Action ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Weak Acid ◽  
Diffusion Method ◽  
Agar Disc

Bioactive properties of solvent extracts of Tasmannia lanceolata, Backhousia citriodora and Syzygium anisatum investigated. The antimicrobial activities evaluated using agar disc diffusion method against two bacteria (Escherichia coli and Staphylococcus aureus) and six weak-acid resistant yeasts (Candida albicans, Candida krusei, Dekkera anomala, Rhodotorula mucilaginosa, Saccharomyces cerevisiae and Schizosaccharomyces pombe). The antioxidant activities determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging and reducing power assays. Quantification of major active compounds using ultra-high performance liquid chromatography. Extracts showed broad-spectrum antifungal activity against weak-acid resistant yeasts in comparison to the standard antifungal agents, fluconazole and amphotericin B. Dekkera anomala being the most sensitive and strongly inhibited by all extracts, while Escherichia coli the least sensitive. Polygodial, citral and anethole are the major bioactive compounds identified in Tasmannia lanceolata, Backhousia citriodora and Syzygium anisatum, respectively. Hexane extracts contain the highest amount of bioactive compounds and demonstrate the strongest antimicrobial activities. Methanol and ethanol extracts reveal the highest phenolic content and antioxidant properties. Fluorescence microscopic results indicate the mechanism of action of Backhousia citriodora against yeast is due to damage of the yeast cell membrane through penetration causing swelling and lysis leading to cell death.

scholarly journals Ascorbic Acid and Polyphenols Mediated Green Synthesis of Silver Nanoparticles from Tagetes erecta L. Aqueous Leaf Extract and Studied Their Antioxidant Properties

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Pankaj Kumar Tyagi ◽  
Shruti Tyagi ◽  
Deepak Gola ◽  
Arvind Arya ◽  
Seyed Abdulmajid Ayatollahi ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Tagetes Erecta ◽  
Aqueous Leaf Extract ◽  
Tagetes Erecta L ◽  
Phenolic And Flavonoid

Silver nanoparticle synthesis of the leaf extract Tagetes erecta L. enriched with ascorbic acid and polyphenols has been investigated. The color of the golden yellow extract has changed to pinkish-brown due to the reduction of Ag+ to the colloidal solution of AgNPs and a sharp absorption peak at 420 nm under the UV-Vis spectrophotometer. In addition, the Fourier Transfer Infrared Spectroscopy (FTIR) estimation was completed in order to recognize and identify the biomolecules present in the extract acting as a reducing and capping agent for the AgNPs. The X-ray diffraction (XRD) peaks at (111), (201), (220), and (311) confirm the presence of monoclinic crystals in the solution. The morphology and size of the particles were provided by transmission electron microscopy (TEM) images of AgNPs. At a scale of 100 nm, synthesized AgNPs were predominantly spherical with a size range of 7-35 nm. In comparison to 7.39 mg/100 g in AgNPs, aqueous leaf extract was 55.14 mg/100 g higher in ascorbic acid. The phenolic and flavonoid content of extract was 52.54 ± 2.15  mg (GAE/100 g) and 15.43 ± 0.34  mg (QE/mL), and the colloidal AgNP solution was 21.45 ± 1.15  mg (GAE/100 g) and 8.05 ± 2.42  mg (QE/mL), respectively. Phenolic and flavonoid contents play a major role as a reducing agent and reduce the precursor AgNO3 into AgNPs. The DPPH scavenging assay also assessed the antioxidant properties of extract and its derived AgNPs. As compared antioxidant value to aqueous leaf extract (mg/mL), higher percentage inhibition (PI) was found in AgNPs and free-radical scavenging activity of extract and AgNPs were directly linked to their concentrations. Results of this research have discovered a higher potential for free-radical scavenging AgNPs and will help to develop new and more potent antioxidants for the treatment of different diseases caused by oxidative stress; the higher antioxidant properties bearing AgNPs might be used.

scholarly journals Evaluation of In Vitro Antioxidant and Antimicrobial Activities of Green Microalgae Trentepohlia umbrina

2012 ◽  
Vol 40 (2) ◽  
pp. 86 ◽  
Author(s):  
Snezana SIMIC ◽  
Marijana KOSANIC ◽  
Branislav RANKOVIC
Keyword(s):  
Superoxide Anion ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Antioxidant And Antimicrobial Activities

The aim of the study is to examine in vitro antioxidant and antimicrobial activities of the methanol extract of the green microalga Trentepohlia umbrina. Antioxidant activity was evaluated by five different methods: free radical scavenging, superoxide anion radical scavenging, reducing power, determination of total phenolic compounds and determination of total flavonoid content. As a result of the study methanol extract of Trentepohlia umbrina had moderate free radical scavenging activity with IC50 values 665.28 ?g/ml. Moreover, the tested extract had effective reducing power and superoxide anion radical scavenging. Total content of phenol and flavonoid in extract were determined as pyrocatechol equivalent, and as rutin equivalent, respectively. The antimicrobial activity was estimated by determination of the minimal inhibitory concentration by the broth microdilution method against five species of bacteria and five species of fungi, including agents of human, animal and plant diseases, mycotoxin producers, and food spoilage agents. Generally, the tested extract had relatively strong antimicrobial activity against the tested microorganisms with minimum inhibitory concentration values ranging from 1.25 to 5 mg/ml. The present study shows that tested methanol extract demonstrated relatively strong antioxidant and antimicrobial activities. It suggests that this microalga may be used as good sources of natural antioxidant and antimicrobial agents.

Antioxidant Properties of Ethanol Extracts from Hohenbuehelia serotina and Armillaria mellea

2012 ◽  
Vol 195-196 ◽  
pp. 342-346
Author(s):  
Hong Fang Ji ◽  
Ling Wen Zhang ◽  
Hai Yan Zhang ◽  
Gui Hai Zhang ◽  
Qing Wang ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Ferrous Ions ◽  
Armillaria Mellea ◽  
Chelating Ability ◽  
Ethanol Extracts

Ethanol extracts from Hohenbuehelia serotina and Armillaria mellea fruit bodies were prepared and investigated for their antioxidant capacities in three different assays, namely, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, reducing power and chelating ability on ferrous ions. Among the two mushrooms, ethanol extracts from A. mellea was more effective than that from H. serotina in scavenging DPPH free radical ability, while ethanol extracts from H. serotina was some higher than that from A. mellea in reducing power. With regard to chelating effect on ferrous ions, at 5 mg/mL, chelating ability of ethanol extracts from H. serotina were 43.17 %, while that from A. mellea was 46.54%. It can be concluded that ethanol extracts from A. mellea and H. serotina were good in antioxidant properties.

EVALUATION OF SALVIA LANATA LEAF EXTRACT FOR ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITY

2020 ◽  
pp. 113-117
Author(s):  
LIPI NOGAI ◽  
TIRATH KUMAR ◽  
PANKAJ LOHUMI
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Paw Edema ◽  
Standard Drug ◽  
Anti Inflammatory

Objective: The work is aimed to evaluate the anti-inflammatory and antioxidant activity of the ethanolic leaf extract of Salvia lanata. Methods: Anti-inflammatory activity of the leaf extract of S. lanata at a dose of 100 mg/kg and 200 mg/kg against the standard drug indomethacin at a dose of 10 mg/kg i.p. was evaluated by carrageenan-induced rat paw edema and protein denaturation method. Antioxidant activity was determined by 1, 1 diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method, reducing power method, and nitric oxide scavenging assay. Results: S. lanata leaf extract showed highly significant dose-dependent efficacy against carrageenan-induced paw edema at a dose of 200 mg/kg and lesser effect at 100 mg/kg. It inhibited heat-induced albumin denaturation with a maximum inhibition of 79.26% at 160 μg/ml. DPPH free radical scavenging activity of extract exhibited inhibition of 25.96%–87.74% within the concentration range of 10 μg/ml–160 μg/ml, nitric oxide assay from 12.26% to 79.22% in the same concentration range. In reducing power assay with an increase in concentrations, an increase in the absorbance of the reaction mixture was observed. Antioxidant activity was compared to standard drug ascorbic acid. Conclusion: The leaf extract of S. lanata has potent anti-inflammatory and antioxidant activity.

scholarly journals PHYTOSYNTHESIS OF SILVER NANOPARTICLES USING HYGROPHILA AURICULATA LEAF EXTRACT AND ASSESSMENT OF THEIR ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES

2018 ◽  
Vol 10 (6) ◽  
pp. 112 ◽  
Author(s):  
Bharathi S. ◽  
S. Kumaran ◽  
G. Suresh ◽  
B. Ramesh ◽  
M.s. Nalina Sundari
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Hydrazyl Radical ◽  
Radical Scavenging Assay ◽  
Agar Well Diffusion Method

Objective: In this study, the phytosynthesis of silver nanoparticles (AgNPs) using leaf extracts of Hygrophila auriculata (HA) and their biological activities was investigated.Methods: The synthesis of AgNPs was done by using the green synthetic method and analyzed by UV-Visible spectroscopy, X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and further characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). The antibacterial activity of HA AgNPs was studied by agar well diffusion method and micro broth dilution method for determination of minimum inhibitory concentration (MIC). The antioxidant potentials of HA AgNPs were detected by Diphenyl-1-picryl hydrazyl radical scavenging assay (DPPH), Phosphomolybdenum assay, reducing power assay.Results: The prepared HA AgNPs showed characteristic absorption peak at 420 nm in the UV-Vis spectrum. FTIR spectra had shown that the biomolecules present in leaf extract were responsible for the reduction and capping material of silver nanoparticles. XRD study showed the particles found to be crystalline in nature, with a face-centered cubic (fcc) structure. TEM and DLS results revealed that the AgNPs were mostly spherical with an average size ranging from ~15-115 nm with a mean diameter of 40.96 nm. The HA AgNPs showed good antibacterial activity and MIC against Staphylococcus aureus (ATCC 6538), Bacillus cereus (NCIM 2106), Pseudomonas aeruginosa (ATCC 9027) and Escherichia coli (ATCC 8739). In agar well diffusion method, the maximum zone of inhibition was found against Pseudomonas aeruginosa (ATCC 9027) with 18 mm and minimum zone of inhibition was found to be against Escherichia coli (ATCC 8739) with 13 mm. The MIC of the HA AgNp was found to be 5µg against all the test organisms. In addition, the Diphenyl-1-picryl hydrazyl radical scavenging assay (DPPH), Phosphomolybdenum assay, reducing power assay revealed they can be used as the potential scavenger against deleterious damages caused by the free radicals.Conclusion: The present study explored that Hygrophila auriculata which are efficient producers of AgNPs and could act as safe and cost-effective with potential antibacterial and antioxidant activities. These findings encourage studying HA AgNP further for their potential biological applications.

scholarly journals QUALITATIVE PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF ELYTRARIA ACAULIS LINDAU (ACANTHACEAE) Research Article

2016 ◽  
Vol 9 (9) ◽  
Author(s):  
Manigandan Murugan ◽  
Kolanjinathan K
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Reducing Power Assay

ABSTRACTObjectives: To analyze the phytochemicals quantitatively and screening the antioxidant efficacy of methanol extracts of Elytraria acaulis plant extractby in vitro.Methods: The total phenols, flavonoids, and tannin contents analyzed by Folin-Ciocalteu method, aluminum chloride colorimetric and calculated asgallic acid equivalents/g (GAE/g) of dry weight. Free radical scavenging activity was screened using 1,1-diphenyl-2-picrylhydrazyl (DPPH), radicalscavenging activities, and ferric reducing power assay was also analyzed.Results: The total phenolic content of the aqueous leaf extract was 46.84 µg GAE/g of extract powder. Significantly similar levels of total flavonoid andtannin contents of the plant were 41.72 and 39.50 µg GAE/g, respectively. The plant extracts showed appreciable free radical scavenging activities atthe highest concentration of 400 µg/mL superoxide anion radical with IC values (107.7±1.081 µg/mL), and for DPPH and considerably high amountof radical scavenging activity was found with very low IC5050 values (4.3±0.88 µg/mL) compared with quercetin.Conclusion: The phenolic and flavonoid compounds provide substantial antioxidant properties which could be effectively used for pharmaceutical,nutraceutical as well as anti-inflammatory applications.Keywords: Phenols, Flavonoids, 1,1-diphenyl-2-picrylhydrazyl, Pharmaceuticals.

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