scholarly journals UJI EFEKTIFITAS FORMULA SPRAY DARI MINYAK ATSIRI HERBA KEMANGI (Ocimum Sanctum L ) SEBAGAI REPELLENT NYAMUK Aedes aegypti

2017 ◽  
Vol 2 (2) ◽  
pp. 189
Author(s):  
Resmi Aini ◽  
Rina Widiastuti ◽  
Nuha Afra Nadhifa
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Essential Oils ◽  
Negative Control ◽  
Repellent Activity ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Control Group Design ◽  
A Value ◽  
Post Test

Dengue fever is transmitted by the bite of Aedes aegypti mosquitoes infected with denguevirus. Preparations antinyamuk much use of harmful chemicals such as DEET (diethylmetatoluamide) and permethrin that can lead to pollution of the environment, leavingno residue and cause resistance to the drug. To determine the activity of essential oils from herbs basil (Ocimum Sanctum L) in the preparation of spray as a mosquito repellent and determine the effective concentrationof the spray.This type of research is True experiment with design Post Test Only Control Group Design. Repellent activity was observed by means of spray is sprayed on the back of the hand probandus, then tested on female mosquitoes with the introduction into the cage containing20 mosquitoes each treatment. Spray essential oils showed repellent activity of Aedes aegypti with a value of correlation r = 0.964 higher concentration of essential oils the fewer mosquitoes that land. Spray herbal essential oil of basil (Ocimum basilicum L) has a repellent activitycompared to the negative control. The effectiveness of the herb basil essential oil sprayobtainedat 74.131 ppm lC50 and lC90 of  775.711 ppm

scholarly journals Uji Efektivitas Sediaan Hand Sanitizer Kombinasi Ekstrak Daun Kemangi (Ocimum Sanctum L) Dan Ekstrak Kulit Jeruk Purut (Citrus Hystrix)

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Indri Kusuma Dewi ◽  
Bambang Yunianto
Keyword(s):  
Confidence Level ◽  
Negative Control ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Hand Sanitizer ◽  
Post Test ◽  
Test Control ◽  
Optimal Formula

Absctract : Antiseptic Activity, Kemangi Leaf- Cytrus Hystrix Leather, Gel Antiseptic (Hand Sanitizer). It is known kemangi leaf had contained saponins, flavonoids and tannins that benefit as antibacterial, while in research by Susanti, 2010 had proven that leather of Citrus hystrix contained essential oil as antibacterial and used as antiseptic in pharmaceutical case. So, to get the optimal formula as hand sanitizer or antiseptic gels used combination two extracts kemangi leaf and Citrus hystrix leather. This research is an experimental research with Post Test Control Group Design to analyze antiseptic activity using the Replica methods with to account the number of colonies of microorganism. Statistic test to the number of colonies of microorganism with Anova Test one way that confidence level 95% and obtained significance 0,000 the result P < 0,05 and continued to Tukey HSD test that confidence level 95%. The result statistic tes showed that have significan differences between negative control with the formula of gel antiseptic combination extract of kemangi leaf and extract Cytrus hystrix leather, so this formula have activity as antiseptic. The most effective variation concentration is concentration extract of kemangi leaf 75% and extract Cytrus hystrix leather 25%.

scholarly journals Uji Daya Tolak Lilin Aromaterapi Minyak Atsiri Serai (Cymbopogon citratus) terhadap Nyamuk Aedes aegypti

2020 ◽  
pp. 21-28
Author(s):  
Anizah Paramitha Dewi ◽  
Novyan Lusiyana
Keyword(s):  
Aedes Aegypti ◽  
Cost Effective ◽  
Negative Control ◽  
Control Group ◽  
Cymbopogon Citratus ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group Design ◽  
Post Test ◽  
Lemongrass Essential Oil

Aromatherapy candle is a safe, effective, and cost-effective repellent. It has a potential repellent effect particularly on mosquitoes. Lemongrass is a potential plant as a natural repellent for Aedes aegypti. The purpose of this study was to determine the repellency effect of lemongrass essential oils against Ae. aegypti. This study was an experimental study with a post-test only with control group design, which was divided into six groups (one negative control and five treatment groups 9%, 10%, 11%, 12%, and 13%) with four repeats. Each group consisted of 20 Ae. aegypti female mosquitoes, so the total number of mosquitoes used was 480. Tested mosquitos were exposed to aromatherapy candles for one hour, and their repellency observed every 15-minute intervals. The average of repellency effect of the negative control and 5 treatment groups at minute 60 were 7.5%; 0%; 33.33%; 66.67%; 66.67%; and 100% respectively. We conclude that the aromatherapy candle of lemongrass essential oil was effective as a 100% repellency effect against Ae. aegypti at a concentration of 13%.

scholarly journals EFEKTIVITAS TANAMAN SEREH WANGI SEBAGAI LARVASIDA NYAMUK Aedes aegypti

2021 ◽  
Vol 13 (1) ◽  
pp. 19-26
Author(s):  
Achmad Farich ◽  
Agung Aji Perdana ◽  
Dian Yunita
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Fourth Instar ◽  
Test Material ◽  
Control Group ◽  
Composition Analysis ◽  
Control Group Design ◽  
Breeding Sites ◽  
Group Design ◽  
Post Test

Dengue Haemorrhagic Fever is an infectious disease caused by the dengue virus which is transmitted mainly through the bite of the Aedes aegypti mosquito. Currently, larvicides are an important strategy in dengue vector control. Larvisides are applied to mosquito breeding sites to kill larvae. This study aims to determine the effectiveness of citronella essential oil as a larvicide for Aedes aegypti. An experimental design with a randomized post-test only control group design was applied in this study using the first to fourth instar larvae of Aedes aegypti as the test material. The concentrations of essential oils applied were 10 ppm, 50 ppm, 100 ppm, 500 ppm and control with 4 replications for 3 different days. A total of 25 Ae aegypti larvae were used in each treatment and observed for 24 hours. Data analysis was performed using univariate and bivariate (probit test and Kruskal Wallis test). The results showed that all concentrations in the treatment group had significant differences with the control group with p < 0.05. This laboratory test also revealed that citronella essential oil was effective to kill the fourth instar larvae of Aedes aegypti with an LC50 of 1.553 mg/L. Citronella essential oil was effective in killing 50% of IVth instar larvae (LT50) within 3.6 minutes. The results of the chemical composition analysis of citronella essential oil using GC-MS showed citronellal, 2,6-octadien-1-ol, 3,7-dimethyl, and citronellol as the most abundant compositions. Citronella essential oil has potential as an effective botanical pesticide to control  Aedes aegypti larvae. Abstrak Demam Berdarah Dengue adalah salah satu penyakit menular yang disebabkan virus dengue yang ditularkan dari seseorang ke orang lain melalui nyamuk Aedes aegypti. Saat ini, larvasida merupakan salah satu strategi penting dalam pengendalian vektor dengue. Tujuan dari penelitian ini adalah untuk melihat efektivitas minyak atsiri sereh wangi sebagai larvasida Aedes aegypti. Metode yang digunakan berupa eksperimental design dengan rancangan randomized post test only control group design dengan menggunakan larva Ae. aegypti instar I sampai IV sebagai material uji. Konsentrasi minyak atsiri yang digunakan yaitu 10 ppm, 50 ppm, 100 ppm, 500 ppm dan kontrol dengan  empat kali pengulangan selama tiga hari berbeda. Masing-masing perlakuan menggunakan 25 larva dan dilakukan pengamatan selama 24 jam. Analisis data dilakukan secara univariat dan bivariat (uji probit dan uji kruskal wallis). Hasil penelitian menunjukkan bahwa semua konsentrasi pada kelompok perlakuan memiliki perbedaan secara bermakna dengan kelompok kontrol p<0,05. Minyak atsiri sereh wangi pada semua konsentrasi perlakuan efektif dalam membunuh larva Ae. aegypti.  Konsentrasi minyak atsiri sereh wangi yang paling baik membunuh 50% (LC50) larva Ae. aegypti instar IV sebesar 1,553 mg/L. Minyak atsiri sereh wangi mampu membunuh larva Ae. aegypti instar IV sebanyak 50% (LT50) pada waktu 3,616 menit. Komponen utama minyak sereh atsiri sereh wangi yang teridentifikasi  dengan GC-MS adalah  citronellal, 2,6-octadien-1-ol, 3,7-dimetil, dan citronellol. Minyak atsiri sereh wangi dapat berpotensi sebagai larvasida botani yang efektif untuk mengontrol nyamuk Ae. aegypti

scholarly journals Ateleia glazioveana and Ocimum basilicum: plants with potential larvicidal and repellent against Aedes aegypti (Diptera, Culicidae)

2021 ◽  
Vol 10 (17) ◽  
pp. e228101724733
Author(s):  
Kelly Alievi ◽  
Giovana Tamara Capoani ◽  
Maike Buzatto ◽  
Daniela Miorando ◽  
Patrícia Zanotelli Serpa ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Negative Control Group ◽  
Negative Control ◽  
Ocimum Basilicum ◽  
Repellent Activity ◽  
Control Group ◽  
Bacillus Thuringiensis Israelensis ◽  
Synthetic Compounds ◽  
Positive Controls

The Aedes aegypti is the main vector of the transmission of diseases such as dengue, yellow fever, Chikungunya, and Zika. The agents used for the control of mosquito involve the use of synthetic compounds, which may cause toxicity and contamination environmental. Aiming to test the effectiveness of natural products, the aim of this study was to evaluate the larvicidal and repellent activity of extracts of Ateleia glazioveana Baill. (timbó) and essential oil of Ocimum basilicum L. (manjericão), against A. aegypti. The activities of hydroalcoholic (HEA) and dichloromethane (DEA) extracts from A. glazioveana against A. aegypti larvae were assessed after exposure for 24, 48, and 72 h, using water and Bacillus thuringiensis israelensis as negative and positive controls, respectively. For repellent evaluation, we investigated the sting attempts of A. aegypti in response to the application of HEA- and DEA-based body creams (5%), as well as an alcoholic spray based on the essential oil from O. basilicum. The repellency of these preparations was compared with negative (vehicle) and positive (DEET) controls. Although HEA showed no apparent larvicidal activity, DEA at concentrations of 150 to 500 mg/ml showed high larvicidal potential after 24 h, compared to the negative control group (p < 0.001). Moreover, the DEA-based cream and the alcoholic spray of O. basilicum based essential oil showed higher repellency compared with the vehicle (84.5% and 70.5%, respectively). Accordingly, it is possible to infer that extracts and preparations of A. glazioveana and O. basilicum exhibit larvicidal and repellent activity against A. aegypti.

scholarly journals Potensi Minyak Atsiri Bunga Lawang (Illicium verum) sebagai Repelen Nyamuk Aedes aegypti

2019 ◽  
pp. 13-22
Author(s):  
Eva Lestari ◽  
Bondan Fajar Wahyudi ◽  
Adil Ustiawan ◽  
Dian Indra Dewi
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Star Anise ◽  
Post Test ◽  
Illicium Verum

Dengue Hemorrhagic Fever (DHF) is still a become public health problem in the world. In 2017, there were  68,407 cases with 493 deaths DHF cases in Indonesia . The incidence rate of DHF in 2017 was 26,10/100,000 population. DHF was caused by dengue virus which was transmitted through the bites of Aedes aegypti. Several types of plants such as stems, leaves, and flowers can be used as natural ingredients for repelling Ae. aegypti, Star anise (Illicium verum) has a chemical compound that can be used as a mosquito repellent. The purpose of this study was to know the potential of star anise essential oil as repellents of Ae. aegypti. This study was an experimental study with post test only with control group design. The experiment to test  the repellency of star anise essential oil was carried out using concentrations of 10%, 20%, 30%, 40%, and 50%  on 3-5 days old female Ae. aegypti. The result of this study showed that repellency index of star anise essential oil at concentration 10%, 20%, 30%, 40% and 50% during 6 hours were 58.1%; 63.51%; 59.95%; 49.45% and 65.32%. Star anise essential oil at concentration 10%, 20%, 30%, 40%, and 50% can provide protection against the bites of Ae. aegypti for 1 until 2 hours.

scholarly journals The Effect of Coffee on The Quantity of Spermatozoa of Diabetic Wistar Rats Inducted By Aloxan

2021 ◽  
Vol 4 (2) ◽  
pp. 11
Author(s):  
Ahmad Alrizaldi ◽  
Riandini Aisyah ◽  
Safari Wahyu Jatmiko
Keyword(s):  
Normal Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Test Results ◽  
Control Group Design ◽  
White Rats ◽  
A Value ◽  
Wistar Strain ◽  
Post Test

Hyperglycemia in people with diabetes mellitus causes oxidative stress which can interfere with the provision of nutrients through blood vessels to the tissues that form spermatozoa so that it interferes with the process of spermatogenesis in the testicular organs. Coffee contains caffeine and chlorogenic acid which functions as an antioxidant to improve spermatogenesis abnormalities which causes decreased number of normal spermatozoa. The purpose of this study was to determine the ability of coffee in improving the number of spermatozoa of alloxan induced wistar white rats.. Methods this study uses Post-test only laboratory experimental research with control group design. Submitting 30 rats divided into 5 groups: normal control (given aquades), negative control (induced alloxan + aquades), setting 1 (induced alloxan + coffee 0.054 gram / 200 gramBB), aid 2 (alloxan induced + coffee 0.108 gram / 200 gramBB ) and treatment 3 (alloxan + coffee induced 0.162 gram / 200 gramBB). Provision of coffee carried out for 14 days, rats terminated on the 15th day. Testicles are taken then observed and counted spermatozoa. One-way ANOVA test results show the value of p = 0.000. In the LSD test between the normal control group (aquades) and negative control (diabetic rats without treatment) had a value of p = 0.042, whereas in the treatment group 1.2.3 with a negative control had a value of p = 0.000. Provision of coffee can increase the number of spermatozoa white wistar strain alloxan induced.

scholarly journals EFIKASI EKSTRAK DAUN ZODIA (Evodia suaveolens) PELARUT HEKSANA DALAM MEMATIKAN LARVA INSTAR III Aedes aegypti

2020 ◽  
Vol 8 (2) ◽  
pp. 195
Author(s):  
Didi Setiyadi ◽  
Martini Martini ◽  
Mochamad Hadi
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Larval Mortality ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Environmental Damage ◽  
P Value ◽  
Control Group Design ◽  
Post Test

DHF is a disease caused by a virus which is transmitted through the bite of the Aedes aegypti mosquito and is one of the health problems in Indonesia. Efforts to decide the transmission of DHF by controlling the population of Aedes aegypti at the larval stage using larvaside temepos. Long-term use of temephos causes Aedes aegypti resistance and environmental damage. One of the plants that has the potential as botanical larvaside is the leaves of zodia (Evodia suaveolens). The purpose of this study was to prove the efficacy of zodia leaves of hexane solvent for killing A. aegypti larvae. This type of research is a pure experiment with the post test only control group design. The research sample is A. aegypti stage III susceptible strain. There are 7 groups consisting of 1 negative control (aquades), positive control (1 ppm temepos) and 5 treatment groups (zodia leaf extract). Data on larval mortality were counted 24 hours after treatment. The results of the one way ANOVA test showed that there were differences in mortality between test groups with a p value: 0.001 α (0.05). Zodia leaf extract probit test results showed LC50-24 hour and LC90-24 hour values of 0,443 ppm and 0,788 ppm. Zodia leaf extract with hexane solvent has efficacy as Aedes aegypti larvicide.

scholarly journals Status Kerentanan Aedes Aegypti Vektor Demam Berdarah Dengue di Kota Padang

2017 ◽  
Vol 6 (2) ◽  
pp. 440
Author(s):  
Kharisma Putra D ◽  
Hasmiwati Hasmiwati ◽  
Arni Amir
Keyword(s):  
Aedes Aegypti ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova

Salah satu upaya untuk mengurangi kasus DBD adalah dengan pengendalian vektor DBD dengan larvisida. Temephos merupakan salah satu insektisida yang telah digunakan lebih dari 30 tahun dan berfungsi mengendalikan larva vektor. Penggunaan temephos yang tidak sesuai aturan dapat menyebabkan penurunan kerentanan pada vektor DBD.  Tujuan penelitian ini  adalah untuk  menilai  status  kerentanan larva Aedes aegypti di tiga kecamatan di Kota Padang. Penelitian ini menggunakan post  test  only  with  control  group design. Telur diambil dan dipelihara di laboratorium hingga mencapai larva instar III/IV. Uji kerentanan untuk temephos dilakukan berdasarkan standar WHO. Hasil penelitian menunjukkan pada Kecamatan Kuranji, kematian larva pada konsentrasi 0,005 mg/L sebesar 10%, 0,01 mg/L sebesar 45%, 0,02 mg/L sebesar 86%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Koto Tangah, kematian larva pada konsentrasi 0,005 mg/L sebesar 24%, 0,01 mg/L sebesar 48%, 0,02 mg/L sebesar 99%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Padang Timur pada konsentrasi 0,005 mg/L didapatkan kematian larva sebesar 12%, pada 0,01 mg/L sebesar 43%, pada 0,02 mg/L sebesar 99%, dan pada 0,03 mg/L sebesar 100%. Hasil uji One way-Anova adalah bermakna dengan nilai p<0,05 pada ketiga kecamatan dan LC99 sedikit diatas 0,02 mg/L. Simpulan penelitian ini adalah status kerentanan Aedes aegypti terhadap temephos di tiga kecamatan berkisar antara rentan dan toleran, belum mencapai resisten sehingga temephos masih dapat digunakan dalam pengendalian vektor DBD.

scholarly journals Mosquito Larvacidal Activity of Zingiber montanum Rhizome Extract Against Aedes aegypti larvae

2019 ◽  
pp. 33-40
Author(s):  
Dwi Sulistia Ningrum ◽  
Siwi Pramatama Mars Wijayanti ◽  
Kuswanto Kuswanto
Keyword(s):  
Aedes Aegypti ◽  
Negative Impact ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Larvicidal Effect ◽  
Natural Insecticide ◽  
Post Test ◽  
Quasi Experimental ◽  
Lc50 And Lc90

Aedes aegypti is the main vector which transmits Dengue virus as causing agent of Dengue Haemmorhagic Fever (DHF). Chemical control of Ae. aegypti have an impact on the environment and humans, also burden a high cost. One of the efforts to reduce the negative impact of synthetic insecticide, which is to find out alternative natural insecticide from plant-based insecticides. The purpose of this research is to determine the killing power of the rhizome bangle extract to Ae. aegypti larvae. This research was a quasi-experimental design with post test only control group design. The concentration of extract rhizome bangle used were 0%; 0.125%; 0.25%; 0.5%; 0.75% and 1%. The mosquito sample used in this study were instar III of Ae. aegypti larvae as many as 600 larvae. Data analysis used univariate and bivariate (probit and Kruskal Wallis test). The results showed that there was an effect of the concentration of extract rhizome bangle against the mortality of Ae. aegypti larvae with p=0,002 (p<0,05). Extract rhizome bangle effectively killed Ae. aegypti larvae with LC50 and LC90 were 0.148 % and 0.338 %, with the most effective concentration is 1%. Based on this research, extract rhizome bangle has a larvicidal effect on Ae. aegypti, so it can be used as an alternative method to minimize the usage of chemical larvicides that easily applied by the community.

scholarly journals PERSALINAN KALA I PADA IBU DENGAN PEMBERIAN PIJAT DAN AROMATERAPI DI PUSKESMAS KABUPATEN KUBU RAYA

2021 ◽  
Vol 6 (2) ◽  
pp. 76
Author(s):  
Dini Fitri Damayanti ◽  
Oon Fatonah Akbarini ◽  
Tuty Sarini
Keyword(s):  
Essential Oil ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Persalinan akan memberikan pengalaman dan pengaruh jangka panjang yang sangat besar terhadap seorang perempuan dalam menjalani masa kehidupan selanjutnya. Beberapa faktor yang mempengaruhi kemajuan proses persalinan yaitu pengaturan posisi pasien, kehadiran pendamping, latihan bernafas, usapan pada punggung. Pijatan pada punggung akan menye- babkan penurunan ketegangan otot dan relaksasi termasuk pada otot abdomen dapat men- gurangi friksi antara rahim dan dinding abdomen. Aromaterapi adalah terapi yang menggu- nakan essential oil atau sari minyak murni untuk membantu menenangkan jiwa dan raga. Secara ilmiah, reaksi terjadi karena wewangian mengirimkan sinyal pada bagian otak yang mengatur emosi sehingga ibu menjadi rileks dan tenang. Kondisi relaksasi yang di alami ibu karena diberikan pijatan atau aromaterapi akan meningkatkan sirkulasi daerah genitalia serta memperbaiki elastisitas servik. Hal ini akan mempercepat pembukaan servik. Peneli- tian quasy experiment dengan pendekatan post test nonequivalent control group design den- gan non probality sampling dengan metode pemilihan sampel yaitu memilih semua individu yang bersalin di Puskesmas Sungai Kakap dan Puskesmas Sungai Durian yang memenuhi kriteria pemilihan. Hasil uji analisis Mann-Whitney menunjukkan bahwa median lama kala I fase aktif pada kelompok pijat lebih singkat 180 (110-240) menit dibanding pada kelompok yang diberikan aromaterapi 225 (120-240) menit.

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