scholarly journals Bioensaios para avaliação da toxicidade aguda, reprodução e ganho de biomassa de minhocas (Eisenia fetida) ambientadas em lodo de esgoto doméstico

2013 ◽  
Vol 8 (1) ◽  
pp. 143-146
Author(s):  
P.R. Dores-Silva ◽  
Landgraf M.D. ◽  
Rezende M.O.O
Keyword(s):  
Eisenia Fetida

TẠO DÒNG VÀ PHÂN TÍCH TRÌNH TỰ GEN SERINE PROTEASE THỦY PHÂN FIBRIN TỪ GIUN QUẾ (Perionyx excavatus)

2013 ◽  
Vol 75 (6) ◽  
Author(s):  
Trần Quốc Dung ◽  
Đặng Phước Hải
Keyword(s):  
Serine Protease ◽  
Eisenia Fetida ◽  
Lumbricus Rubellus ◽  
Perionyx Excavatus

Lumbrokinase của giun quế (Perionyx excavatus) là một enzyme thủy phân fibrin. Trong nghiên cứu này, cDNA mã hóa gen lumbrokinase được khuếch đại với cặp mồi đặc hiệu được thiết kế dựa vào trình tự gen mã hóa lumbrokinase trên GenBank với mã số DQ234061. Đoạn cDNA có kích thước 726 bp được tạo dòng với vector pCR®2.1. Trình tự nucleotide của cDNA được so sánh với trình tự của gen lumbrokinase của các loài giun đất Eisenia fetida (mã số DQ234061), Lumbricus bimastus (mã số AY187629) và Lumbricus rubellus (mã số U25644) trên GenBank và có độ tương đồng lần lượt là 52,02%; 50,06% và 48,03%. Từ khóa: cDNA, lumbrokinase, giun quế (Perionyx excavatus).

Combined acute toxicity of chloride Cd, malathion and acetochlor on Eisenia fetida earthworm

2010 ◽  
Vol 18 (3) ◽  
pp. 562-565 ◽  
Author(s):  
Wen-Peng QIAO ◽  
Yu-Hui QIAO ◽  
Jing ZHAO ◽  
Zhen-Jun SUN
Keyword(s):  
Acute Toxicity ◽  
Eisenia Fetida

scholarly journals Reproduction and biomarker responses of Eisenia fetida after exposure to imidacloprid in biochar-amended soil

Biochar ◽  
2021 ◽  
Author(s):  
Ngitheni Winnie-Kate Nyoka ◽  
Ozekeke Ogbeide ◽  
Patricks Voua Otomo
Keyword(s):  
Eisenia Fetida ◽  
Field Studies ◽  
Application Rate ◽  
Land Application ◽  
Acetylcholinesterase Inhibition ◽  
Beneficial Effects ◽  
Pesticide Pollution ◽  
The One ◽  
The Impact ◽  
Amended Soil

AbstractTerrestrial and aquatic ecosystems are increasingly threatened by pesticide pollution resulting from extensive use of pesticides, and due to the lack of regulatory measures in the developing world, there is a need for affordable means to lessen environmental effects. This study aimed to investigate the impact of biochar amendment on the toxicity of imidacloprid to life-cycle parameters and biomarker responses of the earthworm Eisenia fetida. E. fetida was exposed to 10% biochar-amended and non-amended OECD artificial soils spiked with 0, 0.75, 1.5, 2.25 and 3 mg imidacloprid/kg for 28 days. An LC50 of 2.7 mg/kg was only computed in the non-amended soil but not in the biochar-amended soil due to insignificant mortality. The EC50 calculated in the non-amended soil (0.92 mg/kg) for reproduction (fertility) was lower than the one computed in the biochar amended (0.98 mg/kg), indicating a decrease in toxicity in the biochar-amended substrate. Significant weight loss was observed at the two highest imidacloprid treatments in the non-amended soil and only at the highest treatment in the biochar-amended substrate, further highlighting the beneficial effects of biochar. Catalase activity decreased significantly at the two highest concentrations of non-amended soil. Yet, in the amended soil, the activity remained high, especially in the highest concentration, where it was significantly higher than the controls. This indicated more severe oxidative stress in the absence of biochar. In all non-amended treatments, there was a significant acetylcholinesterase inhibition, while lower inhibition percentages were observed in the biochar-amended soil. In most endpoints, the addition of biochar alleviated the toxic effects of imidacloprid, which shows that biochar has the potential to be useful in soil remediation. However, there is still a need for field studies to identify the most effective application rate of biochar for land application.

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