scholarly journals Kapasitas Stabilisasi Radikal Bebas dan Kelasi Ion Metal Ekstrak Air Daun Vernonia amygdalina Del.

2021 ◽  
Vol 3 (2) ◽  
pp. 79
Author(s):  
Gino Nemesio Cepeda ◽  
Murtiningrum Murtiningrum ◽  
Yuliana Waromi
Keyword(s):  
Vernonia Amygdalina

Tumbuhan obat Vernonia amygdalina dikenal dengan nama “daun afrika” adalah tumbuhan yang berasal dari Afrika. Tumbuhan ini digunakan untuk mengobati malaria, infertilitas, diabetes, gangguan pencernaan dan penyakit transmisi seksual. Tujuan penelitian ini adalah mengevaluasi potensi antioksidan ekstrak air daun afrika yang meliputi kandungan senyawa fenolik, kapasitas stabilisasi radikal bebas melalui transfer atom hidrogen dan kelasi ion metal sebagai prooksidan melalui transfer elektron pada variasi konsentrasi. Proses ekstraksi senyawa bioaktif daun afrika menggunakan metode infusi dengan air panas suhu 90ºC selama 30 menit. Pengujian aktivitas antioksidan ekstrak meliputi penentuan kandungan senyawa fenolik menggunakan metode Folin-Ciocalteu, pengujian kapasitas stabilisasi radikal bebas menggunakan metode  stabilisasi radikal bebas DPPH (1,1-Diphenyl-2-picrylhydrazyl) dan pengujian kapasitas kelasi ion metal menggunakan metode kelasi ion metal Fe+2. Hasil pengujian menunjukkan bahwa ekstrak air daun afrika konsentrasi 0,1-0,5% mengandung senyawa fenolik sebesar 18,81-69.89 µg EAG/ml, kapasitas stabilisasi radikal bebas DPPH sebesar 20,99-41,98% dan kapasitas kelasi ion metal Fe+2 sebesar 8,28-40,71%. Ekstrak air daun afrika kurang berpotensi sebagai sumber antioksidan alami dalam menstabilkan radikal bebas namun demikian sangat efektif mengikat ion metal yang bersifat prooksidan.

Synergistic in-vitro anthelmintic potentials of Vernonia amygdalina Delile stem and Carica papaya Lin. seeds

2017 ◽  
Author(s):  
A Quartey ◽  
A Oppong ◽  
I Ayensu ◽  
J Apenteng ◽  
D Mintah ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Vernonia Amygdalina

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

scholarly journals Growth and yield performances of rice (Oryza sativa var. nerica) after exposure to biosynthesized nanoparticles

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
B. Ikhajiagbe ◽  
F. A. Igiebor ◽  
M. C. Ogwu
Keyword(s):  
Oryza Sativa ◽  
Moringa Oleifera ◽  
Carica Papaya ◽  
Heavy Metal Contamination ◽  
Physiological Stress ◽  
Standard Procedure ◽  
Growth And Yield ◽  
Vernonia Amygdalina ◽  
Ag Nps ◽  
Viable Seeds

Abstract Background Rice (Oryza sativa L.) is a common staple food in Nigeria. However, cultivation is impaired by heavy metal contamination, particularly iron (Fe). This study aimed to investigate the impacts of biosynthesized nanoparticles (NPs) in enhancing the growth and yield components of rice sown in ferruginous soil. Viable seeds of O. sativa var. nerica were sown in ferruginous and non-ferruginous soils. After four weeks, the plants were exposed to foliar sprays of biosynthesized NPs from silver nitrate, using extracts of leaves of Carica papaya, Vernonia amygdalina, Moringa oleifera, and Azadirachta indica; and the flowers of Hibiscus sabderiffa, following standard procedure. The originally prepared stock solution was diluted to give 5, 15, and 30% concentrations of each synthesized NP. Results Results showed that soil ferrugenicity impeded the growth and yield of rice. Azadirachta-synthesized NPs was better enhanced in the ferruginous soils, which might be due to Fe interaction and activities. Moreover, there was increased antioxidant activity in the ferruginous rice compared to the non-ferruginous rice, thus it is evidence that ferrugenicity is a major source of physiological stress for the rice plant. Conclusion The study provided evidence that Ag-NPs can enhance plant yield by huge proportions in ferruginous soil, a condition (ferrugenicity) that was hitherto inimical to yield disposition of rice.

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