scholarly journals Comparative analysis of Qualitative and Quantitative Phytochemical screening, in vitro antioxidant and antibacterial activity of Mangifera indica and Artocarpus heterophyllus

2021 ◽  
Vol 23 (06) ◽  
pp. 1554-1568
Author(s):  
T. Manimekalai ◽  
◽  
Dr. P. Chitra ◽  
Keyword(s):  
Mangifera Indica ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Artocarpus Heterophyllus ◽  
Qualitative And Quantitative ◽  
Phenolic And Flavonoid ◽  
Different Parts ◽  
Screening In Vitro

Medicinal plants have known to play a wide role in the development of human culture. Artocarpus heterophyllus and Mangifera indica are the well-known evergreen tropical fruiting tree families abundantly grown in Asia. Different parts of both the trees such as bark, leaves, fruits, and flowers are used in traditional medicine. The present study aims to screen the qualitative and quantitative phytochemical present, in-vitro antioxidant, and anti-microbial activity of Artocarpus heterophyllus and Mangifera indica leaf extracts and combined extract of both the leaves. The qualitative phytochemical results indicated the presence of phytochemicals such as alkaloids, flavonoids, phytosterols, tannins, and saponins. The quantitative phytochemical results revealed that M.indica has high phenolic and flavonoid content. The Combined extract showed significant antioxidant activity when compared to both the extracts. All the extracts showed significant antimicrobial activity.

scholarly journals Comparative Study of Metabolomic Profile and Antioxidant Content of Adult and In Vitro Leaves of Aristotelia chilensis

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 37
Author(s):  
Karina Andrea Crisóstomo-Ayala ◽  
Ana Belén Sabater-Jara ◽  
Claudia Pérez Manriquez ◽  
Federico Ferreres ◽  
Ángel Gil-Izquierdo ◽  
...  
Keyword(s):  
Total Phenolic ◽  
Qualitative And Quantitative Analysis ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Lipophilic Compounds ◽  
Qualitative And Quantitative ◽  
Pharmaceutical Industries ◽  
Aristotelia Chilensis ◽  
Excellent Source

This work aimed to identify the bioactive compounds present in adult maqui (Aristotelia chilensis) leaves from different stages of development and seasons of the year and compare them with leaves obtained from maqui plants grown in vitro. The qualitative and quantitative analysis of maqui leaf extracts by HPLC-DAD-ESI-MSn showed the presence of different polyphenolic compounds classified into galloyl and caffeoyl quinic acids, ellagitannins and ellagic acid- and flavonoid-derivatives. In general, the total phenolic content of the in vitro samples was higher than that of ex vitro samples, whereas the total flavonoid content was higher in winter basal leaves. Additionally, the analysis by HPLC-MS showed that the extract from spring basal leaves was enriched in quercetin, catechin, kaempferol and 3-caffeoyl quinic acids, while in the in vitro leaves extract, quercetin was not present. As regards lipophilic compounds identified by GC/MS, the samples of in vitro leaves showed a high presence of α-tocopherol and β-sitosterol. In contrast, the samples of adult leaves presented a hight level of linolenic and linoleic acids. These results suggest that maqui leaves could be an excellent source of antioxidants and lipophilic compounds for many industries, such as the nutraceutical and pharmaceutical industries.

scholarly journals Phytochemical, Proximate, In-vitro Antimalarial and Antimicrobial screening of leaf Extracts of Justicia carnea Lindl

2021 ◽  
pp. 55-73
Author(s):  
C. E. Anarado ◽  
V. I. E. Ajiwe ◽  
C. J. O. Anarado ◽  
O. F. Obumselu ◽  
T. U. Onuegbu ◽  
...  
Keyword(s):  
Malaria Parasite ◽  
Therapeutic Use ◽  
Phytochemical Screening ◽  
Leaf Sample ◽  
Leaf Extracts ◽  
Antimicrobial Screening ◽  
Qualitative And Quantitative ◽  
Dependent Inhibition ◽  
Bacillus Spp

Aim: To study the phytochemical, proximate, antimicrobial and anti-malarial activities of leaf extracts of Justicia carnea Methods: The leaf sample of Justicia carnea which has been used in Nigeria folklore in treating malaria, cancer and many other diseases was extracted with methanol, ethyl acetate and n-hexane. The extracts were subjected to qualitative and quantitative phytochemical screening, proximate, anti-malarial and antimicrobial screening. Results: The results showed that alkaloids, flavonoids, glycosides, phenolic compounds were present. Ethy acetate extract also exhibited the highest suppression against malaria parasite in-vitro, more than chloroquine. This inhibition and high abundance of alkaloids and glycosides gave credence to the therapeutic use of the plant as anti-malaria drug. The three extracts showed a concentration-dependent inhibition against S. aureus, Aspergillus spp and Bacillus spp. S. typhi, K. pneumonia, P. aerogenosa and C. albicans were resistant in all concentrations of the extracts. Conclusion: The therapeutic use of the leaves of J. carnea in treatment of malaria was validated. Its antimicrobial activity was also affirmed in some of the tested organisms.

scholarly journals ASSOCIATION BETWEEN TOTAL PHENOL AND FLAVONOID CONTENTS IN ARTOCARPUS HETEROPHYLLUS (JACKFRUIT) BARK AND LEAF EXTRACTS AND LIPOXYGENASE INHIBITION

2020 ◽  
pp. 252-256
Author(s):  
HAFIDZ MAKARIM ILMI ◽  
BERNA ELYA ◽  
ROSITA HANDAYANI
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Bark Extract ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Artocarpus Heterophyllus ◽  
Lipoxygenase Inhibition

Objective: Inflammation is a localized reaction in the tissue in response to injury wherein mediators such as leukotrienes are produced as protectiveresponse leukotrienes are synthesized during the metabolism of arachidonic acid by lipoxygenase and they increase capillary permeability andleukocyte adhesion. Artocarpus heterophyllus is used in many medicines due to its natural anti-inflammatory activity. The aim of this study was to usethe bark and leaf extracts of A. heterophyllus to obtain the most active fraction that could inhibit lipoxygenase and to investigate the total phenol andflavonoid levels in these extracts.Methods: Barks and leaves were extracted by a multistage reflux method. An in vitro lipoxygenase inhibition assay was performed by measuring thetotal phenol and flavonoid contents for each fraction.Results: Ethanol extract was found to be the most active extract in each fraction. IC50 values obtained in the two most active fractions were 31.82μg/mL in the ethyl acetate of bark extract and 46.61 μg/mL in the ethyl acetate of leaf extract. This value was higher than that of apigenin standardwhich has an IC50 value of 2.08 μg/mL. The highest phenol content was present in the ethanol leaf extract with 404.903 mg gallic acid equivalent/gextract. Meanwhile, the highest flavonoid content was found in the ethyl acetate of bark extract with 372.362 mg QE/g extract.Conclusion: The bark and leaf extract tests showed that the increase in the total phenol or flavonoid content was proportional to the increase in theenzyme inhibitory activity.

scholarly journals PHYTOCHEMICAL ANALYSIS AND EVALUATION OF IN VITRO IMMUNOMODULATORY ACTIVITY OF RHODODENDRON ARBOREUM LEAVES

2018 ◽  
Vol 11 (8) ◽  
pp. 123
Author(s):  
Pramod Rawat ◽  
Rakesh Kumar Bachheti ◽  
Navin Kumar ◽  
Nishant Rai
Keyword(s):  
Ursolic Acid ◽  
Aqueous Extract ◽  
Methanolic Extract ◽  
Dry Weight ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Rhododendron Arboreum ◽  
Phenolic And Flavonoid

Objectives: The objectives of this study are to analyze phytoconstituents in Rhododendron arboreum leaves (qualitatively and quantitatively) and to estimate the immunomodulatory effects of extracts using different in vitro methods for intracellular and phagocytic killing potency of neutrophils, which are subsequent, involved in the process of phagocytosis by neutrophils.Methods: R. arboreum leaves extracts were separately extracted in 100% methanol, 50% methanol, and aqueous by a hot percolation technique using Soxhlet apparatus and concentrated with a vacuum rotary evaporator apparatus. The percentage yield of the extracts wares calculated. The extract was used to analyze phytoconstituents qualitatively, and total phenolic and flavonoid content were estimated. In another study, already purified methanolic extract of R. arboreum leaves, namely cytoplasmic male sterility (CMS-3) (kaempferol), RAM fr2, and transcranial magnetic stimulation (TMS-10) (ursolic acid) was used to evaluate immunomodulatory activities by nitroblue tetrazolium test, phagocytosis of killed Candida albicans.Results: Phytochemical screening of the extracts showed the presence of major classes of phytochemicals, i.e., phytosterols, flavonoids, phenols, alkaloids, carbohydrate, glycoside, sterols and steroids, terpenoids, and tannin while saponin was absent in all extract. The percent yield of leaves extract was 12.97%. Total phenolic content (TPC) expressed as gallic acid equivalents (GAE) ranged 1327.5±0.009 (in 100% methanolic extract), 1309.5±0.011 (in 50% methanolic), and 1468.5±0.006 mg GAE g−1 dry weight (in aqueous extract). Total flavonoid content expressed as rutin equivalents (RE) ranged 219±0.025 (in 100% methanolic extract), 184±0.02 (in 50% methanolic), and 262±0.58 mg (in aqueous extract) mg RE g−1 dry weight. R. arboreum purified extract CMS-3 (kaempferol), RAM fr2, and TMS-10 (ursolic acid) stimulated the neutrophils to phagocytic activity to the extent of 67.67±2.08%, 40.3±1.5%, and 79.67±0.57%, respectively. The above-said extracts stimulated the phagocytosis of killed C. albicans. The mean particle numbers for CMS- 3, RAM fr2, and TMS-10 were found to be 6.7, 6.3, and 7, respectively, at concentration 1 mg/ml.Conclusion: The phytochemical screening of all the extracts showed the presence of various phytochemicals that are biologically important. The total phenolic and flavonoid content of the plant are comparable to other medicinal plants. Even though the enormous progress on the phytochemistry and pharmacology of R. arboreum have been made, there still require more conclusive studies on the safety, efficacy, and in vivo toxicity of extracts and pure compounds to gain a better understanding.

QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT EVALUATION OF PERGULARIA DAEMIA

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
GITA MISHRA ◽  
HEMESHWER KUMAR CHANDRA ◽  
NISHA SAHU ◽  
SATENDRA KUMAR NIRALA ◽  
MONIKA BHADAURIA
Keyword(s):  
Reducing Sugars ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
The Family ◽  
Ethanolic Extracts ◽  
Antioxidant Evaluation ◽  
In Vitro Antioxidant Activity

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

Phytochemical analysis and salivary amylase inhibition activities of Carica papaya leaf and Garcinia mangostana pericarp extracts and partially purified fractions

2017 ◽  
Vol 6 (1) ◽  
pp. 34
Author(s):  
Michael Russelle Alvarez ◽  
Paolo Robert Bueno ◽  
Raymond Oliver Cruz ◽  
Richard Macapulay ◽  
Francis Jayson Vallesfin ◽  
...  
Keyword(s):  
Crude Extract ◽  
Carica Papaya ◽  
Uv Light ◽  
Digestive Enzyme ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Salivary Amylase ◽  
Garcinia Mangostana ◽  
Leaf Extracts

Plant-derived digestive enzyme inhibitors particularly those targeted to carbohydrate metabolism has been the focus of recent studies as natural supplements for weight control and diabetes. The present study explores the salivary amylase inhibition activity of Garcinia mangostana (Linn.) pericarp extracts and Carica papaya (Linn.) leaf extracts and fractions, as well as perform phytochemical screening and quantification, and thin layer – and high performance liquid chromatographic profiling. ­Results show that crude extracts and purified fractions were able to inhibit salivary amylase, with C. papaya fraction 1 being the most active at 30.89% inhibition. Phytochemical screening of all extracts tested ­positive for tannins, glycosides, phenolics, flavonoids and alkaloids. Quantification of phenolics showed that extracts contained high levels of phenolics, with C. papaya crude extract having the highest content with 219.0±12.7 mg GAE/g extract followed by G. mangostana crude extract with 247.1±18.0 mg GAE/g extract. Quantification of total flavonoids also showed C. papaya crude extract to contain the highest content with 55.12±0.679 mg QE/g extract. All extracts contained negligible alkaloid content, though. HPLC and TLC profiling showed several peaks and bands, when viewed in 210 nm and UV light, respectively. These results demonstrate in vitro the salivary amylase inhibitory activity of both plants and their potential as antidiabetic drug candidates; however, further studies need to be done, like isolation and structure elucidation of active components and toxicity assays. Keywords: Amylase inhibition, phytochemical quantification, Carica papaya, Garcinia mangostana

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals Establishing Relationship between Vitamins, Total Phenolic and Total Flavonoid Content and Antioxidant Activities in Various Honey Types

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4399
Author(s):  
Norhasnida Zawawi ◽  
Pei Juin Chong ◽  
Nurul Nadhirah Mohd Tom ◽  
Nurkhairina Solehah Saiful Anuar ◽  
Salma Malihah Mohammad ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Extrinsic Factors ◽  
Biochemical Compounds ◽  
Phenolic And Flavonoid

Honey is a well-known natural sweetener and is rich in natural antioxidants that prevent the occurrence of oxidative stress, which is responsible for many human diseases. Some of the biochemical compounds in honey that contribute to this property are vitamins and phenolic compounds such as phenolic acids and flavonoids. However, the extent to which these molecules contribute towards the antioxidant capacity in vitro is inconsistently reported, especially with the different analytical methods used, as well as other extrinsic factors that influence these molecules’ availability. Therefore, by reviewing recently published works correlating the vitamin, total phenolic, and flavonoid content in honey with its antioxidant activities in vitro, this paper will establish a relationship between these parameters. Based on the literature, vitamins do not contribute to honey’s antioxidant capacity; however, the content of phenolic acids and flavonoids has an impact on honey’s antioxidant activity.

Sign in / Sign up

Export Citation Format

Share Document