scholarly journals Metallothionein Expression in Estuarine Catfish (Arius thalassinus) Kidney on Exposure to Heavy Metals (Cd, Pb, Cu and Zn) Concentration

2021 ◽  
Vol 23 (06) ◽  
pp. 186-195
Author(s):  
Ahmed A ◽  
◽  
Ismail A ◽  
Omar H ◽  
Zulkifli S ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Aquatic Environment ◽  
Kidney Tissue ◽  
Pollution Assessment ◽  
Small Fish ◽  
Zn Concentration ◽  
Highly Sensitive ◽  
Primary Mouse ◽  
Immunohistochemical Techniques ◽  
Potential Use

Immunohistochemical techniques have been used to identify Metallothionein3 (MT3) expression specifically to detect MT3 levels in the kidney. The techniques employed are highly sensitive, thus allowing the detection of the very low amount of proteins with the use of antibodies. The present study aimed at identifying the MT3 expressions and localizations in the kidney tissue of estuarine catfish Arius thalassinus from Kuala Gula using immunohistochemical methods. Primary mouse monoclonal anti-MT3 (1F11), primary antibody (1:2000), secondary antibody, and Goat anti-mouse IgG2 (1:2000) were used. MT3 bands were detected using (Santa Cruz kit USA method). Heavy metals (Cd, Pb, Zn, and Cu) were determined using aqua regia’s methods followed by analyses with AAS. The results showed high expressions of Metallothionein3 (MT3) with an increasing concentration of heavy metals. Large catfish A. thalassinus had a higher accumulation of heavy metals concentrations, therefore, exhibiting higher expressions than smaller-sized fish. The concentration of heavy metals in fishes were: Zn (284.00 ±29.7, 259.32± 23.2 and 240.90±20.3 μg/g-1 d.w), Cu (7.00±1.7, 6.90±1.3 and 5.60±1.8 μg/g-1 d.w), Pb (66.80±10.8, 35.31±6.4 and 26.02±6.9 μg/g-1 d.w) and Cd (1.32 ± 0.12, 1.19 ±0.12 and 1.20± 0.09 μg/g-1 d.w) in large, medium and small fish respectively. The results of this study suggest its suitability as a biomarker candidate for pollution assessment in the aquatic environment. The different MT3 expressions in different sized fish and different polluted places showed the potential use of MT3 as a biomonitoring biomarker for heavy metals exposure in an aquatic environment.

COPPER AND ZINC INDUCED CHANGES IN SOYBEAN (Glycine max (L.) Merr.)

1970 ◽  
pp. 09
Author(s):  
K. SANKAR GANESH ◽  
P. SUNDARAMOORTHY
Keyword(s):  
Heavy Metals ◽  
Glycine Max ◽  
Aquatic Environment ◽  
Morphological Traits ◽  
Industrial Activities ◽  
Copper And Zinc ◽  
Glycine Max L ◽  
Zinc Concentrations ◽  
Induced Changes

Heavy metals are one of the most important pollutants released to the aquatic environment by the various industrial activities. The use of these wastewater for irrigation results accumulation of heavy metals in soil and plants. So, the present investigation deals with the various concentrations (0, 5, 10, 25, 50, 100, 200 and 300 mg/l) of copper and zinc on germination studies of soybean. The different concentrations of copper and zinc were used for germination studies. The seedlings were allowed to grow upto seven days. The studied morphological traits increased at 5 mg/l concentration and these parameters are gradually decreased with the increase of copper and zinc concentrations.

scholarly journals Highly sensitive and selective detection of PCB 77 using an aptamer-catalytic hairpin assembly in an aquatic environment

RSC Advances ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 5506-5511
Author(s):  
Lin Yuan ◽  
Qiang Fu ◽  
Maojuan Zhou ◽  
Yunqian Ma ◽  
Lihua Zang ◽  
...  
Keyword(s):  
Aquatic Environment ◽  
Selective Detection ◽  
Fluorescence Sensing ◽  
Highly Sensitive ◽  
Catalytic Hairpin Assembly

The fluorescence sensing strategy was used to detect PCB77 based on the aptamer-complex and catalytic hairpin assembly.

ZIRCONIUM LOADED BANANA STEM FIBERS AS ADSORBENT FOR RECOVERY OF HG(II)

2016 ◽  
Vol 78 (8-3) ◽  
Author(s):  
Nurrulhidayah Salamun ◽  
Sugeng Triwahyono ◽  
Aishah Abdul Jalil
Keyword(s):  
Heavy Metals ◽  
Aquatic Environment ◽  
Active Sites ◽  
Low Cost ◽  
Mercury Removal ◽  
Cellulose I ◽  
Xrd Pattern ◽  
Crystal Phases ◽  
Good Possibility ◽  
Banana Stem

Mercury is one of the most toxic pollutants which pose a great threat to both human health and organism security. A great deal of research over recent decades has been motivated by the requirement to lower the concentration of these heavy metals in water and the need to develop low cost techniques which can be widely applied for heavy metals remediation. Adsorption is by far the most reliable technologies for removing mercury from water. In this study, banana stem fibers, a natural biomass was loaded with zirconium (IV) to investigate its feasibility for mercury removal from an aquatic environment. The XRD pattern for both BSF-HCl and Zr/BSF-HCl exhibited mainly the cellulose I structure which consists of two distinct crystal phases. The FESEM images illustrated the presence of relatively well organized, pronounced and uniform cavities distributed around the surface, indicated a good possibility for the metal ions to be adsorbed. The result shows that Hg (II) adsorption capacity increased from 45 to 72 mg/g after the immobilization of Zr due to increase in the active sites on the adsorbent. 

scholarly journals Highly Sensitive AgNP/MWCNT/Nafion Modified GCE-Based Sensor for the Determination of Heavy Metals in Organic and Non-organic Vegetables

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Shirley Tiong Palisoc ◽  
Michelle Tiamzon Natividad ◽  
Nico De Jesus ◽  
Joshua Carlos
Keyword(s):  
Heavy Metals ◽  
Highly Sensitive ◽  
Organic Vegetables
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