scholarly journals CHARACTERISATION OF AN ANTARCTIC YEAST, Glaciozyma antarctica PI12

2020 ◽  
pp. 89
Author(s):  
Teoh Chul Peng ◽  
Koh Soon Peng ◽  
Clemente Michael Wong Vui Ling
Keyword(s):  
Stationary Phase ◽  
Agar Medium ◽  
Hydrolytic Enzymes ◽  
Optimal Growth ◽  
Psychrophilic Yeast ◽  
Yeast Peptone Dextrose ◽  
Antarctic Yeast ◽  
Glaciozyma Antarctica Pi12 ◽  
A Chain ◽  
Longterm Survival

Glaciozyma antarctica PI12 is a psychrophilic yeast isolated from Antarctica. It has an optimal growth in yeast peptone dextrose (YPD) and yeast mould (YM) broth media but not in potato dextrose (PD) broth medium. Early phase G. antarctica PI12 cells had elongated-shape and became oval-shaped as they aged. G. antarctica PI12 exhibited bipolar budding and formed a chain of cells during the lag and early exponential phases. The number of chains decreased as the yeast aged. It appeared mainly as a single cell at the stationary phase, and a small number of them still produced buds. Some cells at the stationary phase entered the quiescence state (G0) as a longterm survival strategy. The G. antarctica PI12 cell size decreased when they entered the stationary phase. G. antarctica PI12 was found to produce hydrolytic enzymes, chitinase, cellulase, mannanase, and xylanase. A higher glucose concentration of 2% in the PD agar medium inhibited the activities of chitinase but not the cellulase, mananase and xylanase.

In silico analysis of glucoamylase from a psychrophilic yeast, Glaciozyma antarctica PI12

2015 ◽  
Author(s):  
Siti Nur Hasanah Mohd Yusuf ◽  
Farah Diba Abu Bakar ◽  
Nor Muhammad Mahadi ◽  
Abdul Munir Abdul Murad
Keyword(s):  
In Silico ◽  
In Silico Analysis ◽  
Psychrophilic Yeast ◽  
Glaciozyma Antarctica Pi12 ◽  
Silico Analysis

scholarly journals Bioinformatics survey of the metal usage by psychrophilic yeast Glaciozyma antarctica PI12

Metallomics ◽  
2015 ◽  
Vol 7 (1) ◽  
pp. 156-164
Author(s):  
Pik Mun Foong ◽  
Roghayeh Abedi Karjiban ◽  
Yahaya M. Normi ◽  
Abu Bakar Salleh ◽  
Mohd Basyaruddin Abdul Rahman
Keyword(s):  
Psychrophilic Yeast ◽  
Glaciozyma Antarctica Pi12 ◽  
Bioinformatic Approaches

The psychrophilic metallome of Glaciozyma antarctica PI12 predicted by bioinformatic approaches.

Thermal stress responses in Antarctic yeast, Glaciozyma antarctica PI12, characterized by real-time quantitative PCR

Polar Biology ◽  
2012 ◽  
Vol 36 (3) ◽  
pp. 381-389 ◽  
Author(s):  
Sook Yee Boo ◽  
Clemente Michael Vui Ling Wong ◽  
Kenneth Francis Rodrigues ◽  
Nazalan Najimudin ◽  
Abdul Munir Abdul Murad ◽  
...  
Keyword(s):  
Thermal Stress ◽  
Real Time ◽  
Quantitative Pcr ◽  
Stress Responses ◽  
Real Time Quantitative Pcr ◽  
Antarctic Yeast ◽  
Glaciozyma Antarctica Pi12

PNA-nucleic acid complexes. Structure, stability and dynamics

1996 ◽  
Vol 29 (4) ◽  
pp. 369-394 ◽  
Author(s):  
Magdalena Eriksson ◽  
Peter E. Nielsen
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Hydrolytic Enzymes ◽  
Living Cells ◽  
Structure Stability ◽  
Peptide Chemistry ◽  
Cell Extracts ◽  
Phosphate Backbone ◽  
A Chain ◽  
Nucleic Acid Analogues

Growing interest in gene targeting drugs has inspired the development of a multitude of nucleic acid analogues, many of which feature substitutions in the phosphodiester moiety of the backbone (reviewed by Mesmaeker et al. 1995 and Nielsen, 1995). Peptide nucleic acid (PNA) is an example of a more radical redesign of DNA. The entire sugar-phosphate backbone is substituted by a chain of peptide-like N-(2-aminoethyl)glycine units so that an achiral and uncharged DNA-mimic is obtained (Fig. 1; Nielsen et al. 1991). The synthesis is based on standard peptide chemistry (Christensen et al. 1995) and has been automated. PNA can relatively easily be modified to include modifications of the backbone as well as of the bases (Hyrup & Nielsen, 1996). PNA is chemically stable and, in contrast to natural nucleic acids and peptides, PNA is expected to remain intact in living cells since it is not a substrate for natural hydrolytic enzymes and is not degraded by cell extracts (Demidov et al. 1994).

Cloning and expression of phosphoglycerate mutase from the psychrophilic yeast, Glaciozyma antarctica PI12

2015 ◽  
Author(s):  
Nardiah Rizwana Jaafar ◽  
Farah Diba Abu Bakar ◽  
Abdul Munir Abdul Murad ◽  
Nor Muhammad Mahadi
Keyword(s):  
Cloning And Expression ◽  
Phosphoglycerate Mutase ◽  
Psychrophilic Yeast ◽  
Glaciozyma Antarctica Pi12

Characterization of Afp1, an antifreeze protein from the psychrophilic yeast Glaciozyma antarctica PI12

Extremophiles ◽  
2012 ◽  
Vol 17 (1) ◽  
pp. 63-73 ◽  
Author(s):  
Noor Haza Fazlin Hashim ◽  
Izwan Bharudin ◽  
Douglas Law Sie Nguong ◽  
Sakura Higa ◽  
Farah Diba Abu Bakar ◽  
...  
Keyword(s):  
Antifreeze Protein ◽  
Psychrophilic Yeast ◽  
Glaciozyma Antarctica Pi12

scholarly journals (-)-Glaciantarcin, a New Dipeptide and Some Secondary Metabolites from the Psychrophilic Yeast Glaciozyma antarctica PI12

2018 ◽  
Vol 47 (11) ◽  
pp. 2693-2698
Author(s):  
Andi R. Rosandy ◽  
Chian Ying Yeoh ◽  
Yoke Kqueen Cheah ◽  
Seng Joe Lim ◽  
Jalifah Latip ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Psychrophilic Yeast ◽  
Glaciozyma Antarctica Pi12

Biodiversity and enzymes bioprospection of Antarctic filamentous fungi

2018 ◽  
Vol 31 (1) ◽  
pp. 3-12 ◽  
Author(s):  
M.M. Martorell ◽  
L.A.M. Ruberto ◽  
P.M. Fernández ◽  
L.I.C. De Figueroa ◽  
W.P. Mac Cormack
Keyword(s):  
Filamentous Fungi ◽  
Extracellular Enzymes ◽  
Hydrolytic Enzymes ◽  
Optimal Growth ◽  
Nutrient Cycle ◽  
Optimal Growth Temperature ◽  
Cold Environments ◽  
Fungal Isolates ◽  
Cold Active ◽  
The Antarctic

AbstractAntarctica is one of the most suitable locations for the bioprospecting of psychrotrophic fungi, which play a key role in the nutrient cycle and organic material mineralization in cold environments. These actions mainly take place via the production of several cold-active extracellular enzymes. The aim of this study was to investigate the diversity of filamentous fungi from King George Island (25 De Mayo Island), Antarctica and their ability to produce extracellular hydrolytic enzymes at low temperatures. A total of 51 fungal isolates were obtained from 31 samples. Twelve genera were identified, with seven among the Ascomycota (Cadophora, Helotiales, Monographella, Oidodendron, Penicillium, Phialocephala, Phialophora, Phoma and Pseudogymnoascus), one Basidiomycota (Irpex) and two Mucoromycota (Mortierella and Mucor). Monographella lycopodina and Mucor zonatus, not previously reported in Antarctica, were identified. Nine isolates could not be identified to genus level and may represent novel species. Most of the studied fungi were psychrotrophic (76.5%). Nevertheless, only five isolates were able to grow at 35°C, 15°C being the optimal growth temperature for 65% of the fungal isolates. Results from enzyme production at low temperature revealed that the Antarctic environment contains metabolically diverse fungi, which represent potential tools for biotechnological applications in cold regions.

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