scholarly journals High-resolution magnified endoscopy combined with flexible spectral imagining colour enhancement techniques in the diagnosis of Helicobacter pylori disease

2019 ◽  
Vol 14 (3) ◽  
pp. 202-210
Author(s):  
Omer Binicier ◽  
Gozde Hakim ◽  
Sadiye Unlu ◽  
Omer Topalak
Keyword(s):  
Helicobacter Pylori ◽  
High Resolution ◽  
Magnified Endoscopy

Detection of Helicobacter pylori in virtual slides requires high resolution digitalisation

2021 ◽  
pp. jclinpath-2020-207378
Author(s):  
Arnaud Uguen
Keyword(s):  
Helicobacter Pylori ◽  
High Resolution ◽  
Digital Pathology ◽  
Histopathological Analysis ◽  
Histopathological Diagnosis ◽  
Low Resolution ◽  
Glass Slides ◽  
Sydney System ◽  
Kappa Value ◽  
Gastric Biopsies

To diagnose Helicobacter pylori (HP) infection and its related mucosal injuries requires the histopathological analysis of gastric biopsies. The move from glass slides interpretation towards digital pathology implies technical choices to maintain the performances of histopathological diagnosis. The intra-rater agreement in assessing gastritis diagnostic criteria between glass slides, low resolution and high resolution digital slides in the subject of the present study. One hundred gastric biopsies were re-assessed by a single digestive pathologist on glass slides and digitalised slides at low resolution (ie, x20 magnification and single focus without z-stack) and high resolution (ie, x40 magnification with seven focus levels and z-stack) about the criteria of the updated Sydney system and the detection of HP. Inter-analyses agreement were very good (Kappa values>0.81) for every criteria but slightly inferior (ie, Kappa values<0.9) comparing glass slides interpretations with low resolution digital slides-based ones. Indeed, some HP infections were misdiagnosed using x20 magnification histochemical stained digitalised slides (p<0.05). At the opposite, anti-HP immunohistochemistry slides and/or x40 magnification digitalisation permitted to maintain almost perfect concordance in diagnosis (Kappa value>0.9). As mentioned in current guidelines, a high resolution x40 magnification digitalisation must be favoured in order to avoid some misdetection of microorganisms as HP.

Diagnosis of Nonerosive Reflux Disease: High Resolution Magnified Endoscopy and Fujinon Intelligent Chromo Endoscopy (FICE)

2011 ◽  
Vol 140 (5) ◽  
pp. S-251
Author(s):  
Sadettin Hülagü ◽  
Altay Celebi ◽  
Goktug Sirin ◽  
Omer Senturk ◽  
Ugur Korkmaz ◽  
...  
Keyword(s):  
High Resolution ◽  
Reflux Disease ◽  
Nonerosive Reflux Disease ◽  
Magnified Endoscopy

Novel high resolution melt curve assay for the analysis of predominance of Helicobacter pylori clarithromycin resistance

2019 ◽  
Vol 77 (4) ◽  
Author(s):  
Doron Boltin ◽  
Olga Ashorov ◽  
Lucie Benejat ◽  
Dalal Hamouda ◽  
Rachel Gingold Belfer ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
High Resolution ◽  
23S Rrna ◽  
Rrna Gene ◽  
High Resolution Melt ◽  
Clarithromycin Resistance ◽  
Stool Antigen ◽  
Stool Samples ◽  
Antigen Tests ◽  
H Pylori

ABSTRACT Clarithromycin resistance is the most common cause of Helicobacter pylori treatment failure and it is attributed to three point mutations, A2142G, A2142C and A2143G, within the 23S rRNA gene. We aimed to determine the prevalence of H. pylori clarithromycin resistance using a novel high resolution melt assay. A total of 151 stool samples were collected from treatment-naïve patients with general gastric discomfort who also performed 13CO2 breath tests. Stool antigen tests were also performed on 126 of the 151 stool samples collected. Bacterial DNA was extracted from the stool and analyzed by comparing it with four reference plasmids incorporating the three mutations and the wild type (WT) sequences. The melt assay detected 106 H. pylori positive samples, of which 54 had a WT sequence, and 52 had a point mutation associated with clarithromycin resistance, including A2142G in 10, A2142C in 13, A2143G in 18 and heterozygosity (multiple peaks) in 11. Compared with the gold standards (13CO2 breath and stool antigen tests), the melt assay had a sensitivity of 100% and 99% and a specificity of 82% and 78%, respectively. Therefore, our stool-based molecular assay is able to identify H. pylori infection and clarithromycin resistance. It could be used for screening prior to administration of clarithromycin eradication therapy.

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