scholarly journals Clinical immunology Whole blood neutrophil chemiluminescence in children with diabetes depending on their clinical condition

2012 ◽  
Vol 4 ◽  
pp. 345-349 ◽  
Author(s):  
Magda Kaczmarek ◽  
Aleksandra Lewandowicz-Uszyńska ◽  
Zdzisława Iwanicka ◽  
Adam Jankowski
Keyword(s):  
Whole Blood ◽  
Clinical Condition ◽  
Clinical Immunology ◽  
Blood Neutrophil

A comparison of whole blood neutrophil chemiluminescence measured with cuvette and microtitre plate luminometers

Luminescence ◽  
2002 ◽  
Vol 17 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Anton�n Lojek ◽  
Luk�?? Kubala ◽  
Hana ??�??ov� ◽  
Milan ??�??
Keyword(s):  
Whole Blood ◽  
Blood Neutrophil ◽  
Microtitre Plate

Cyclosporine concentrations in blood after liver transplantation: correlation of immunoassay results with clinical events.

1989 ◽  
Vol 35 (4) ◽  
pp. 564-568 ◽  
Author(s):  
M C Haven ◽  
L M Sobeski ◽  
R A Earl ◽  
R S Markin
Keyword(s):  
Liver Transplantation ◽  
Whole Blood ◽  
Clinical Condition ◽  
Fluorescence Polarization ◽  
Clinical Utility ◽  
Correlation Coefficients ◽  
Fluorescence Polarization Immunoassay ◽  
Excretory Function ◽  
Clinical Events ◽  
Concentration Result

Abstract To investigate the clinical utility of immunoassays for cyclosporine and metabolites in plasma and whole blood, we monitored 25 patients after orthotopic liver transplantation. We compared cyclosporine as measured by TDx fluorescence polarization immunoassay (of both plasma and whole blood) and by two polyclonal radioimmunoassays (from Sandoz and INCSTAR). We found considerable differences in measured cyclosporine concentrations, which were dependent on method, matrix, and clinical condition. Correlation coefficients between results by the various methods for samples from individual patients ranged from 0.825 to 0.996. The three methods used for monitoring cyclosporine in whole blood gave proportional results (Sandoz less than INCSTAR less than TDx) in individual patients, but results for the two methods for plasma sometimes differed by more than 100%. In some cases, ratios of plasma cyclosporine concentration (result by TDx/result by Sandoz) were correlated with disturbances in hepatic excretory function or kidney function. This ratio may be useful in monitoring for nephrotoxicity.

scholarly journals PENILAIAN UJI TROPONIN I DENGAN POINT OF CARE TESTING

2018 ◽  
Vol 22 (2) ◽  
pp. 114
Author(s):  
Sheila Febriana ◽  
Asvin Nurulita ◽  
Uleng Bahrun
Keyword(s):  
Whole Blood ◽  
Clinical Condition ◽  
Troponin I ◽  
Task Force ◽  
Point Of Care ◽  
Pearson Correlation ◽  
World Health ◽  
P Value ◽  
Point Of Care Testing ◽  
The Mean

Troponin I is a cardiac biomarker recomended by The Third Global Myocardial Infarction Task Force World Health Organisation(WHO). Troponin plays a central role as a relevant biomarker that require reliable samples, methods, device and efficiency of time.Selecting the device, methods and sample used in the assay may affect the results and turn arround time. The aim of this study is toknow troponin I result using Point of care Testing device with a flourescence immunoassay methods using whole blood and laboratorybasedanalysis device with Enzyme-Linked Fluorescent Assay (ELFA) methods using serum by evaluation. Cross sectional study was heldon 50 subjects in Wahidin Sudirohusodo hospital during the period between July-August 2015, those who suspected suffering acutecoronary syndome (ACS) and underwent troponin I test ordered by the physician and also had whole blood sample. The subjects arearound 51.96±12.80 year old and most of them are men (62%). The mean consentration of troponin I with laboratoric-based analysis is0.50±1.69 μg/L and with POCT is 0.51±1.77. The Pearson correlation test shows the correlation (r) is 0.99 with the p value is <0.001.Bland and Altman methods show the mean difference between two assays is 0.014μg/L (95% confidence interval, -0.015; 0.043) withthe limit of agreement -0.19 to 0.22. Based on this study, it can be concluded that troponin I assay using POCT device can be used tosupport ACS diagnosis precisely and rapidly. It is suggested to perform futher study with concern on the patient’s clinical condition aswell as the diagnosis, so it can evaluate the device performance to measure troponin I levels consistently with the clinical condition.

scholarly journals Whole blood neutrophil gelatinase–associated lipocalin predicts acute kidney injury in burn patients

2015 ◽  
Vol 196 (2) ◽  
pp. 382-387 ◽  
Author(s):  
Soman Sen ◽  
Zack R. Godwin ◽  
Tina Palmieri ◽  
David Greenhalgh ◽  
Amanda N. Steele ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Whole Blood ◽  
Kidney Injury ◽  
Burn Patients ◽  
Blood Neutrophil ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Neutrophil Gelatinase

Fine Structure of Platelet Interaction with a New Microcrystalline Collagen Preparation

1974 ◽  
Vol 32 ◽  
pp. 58-59
Author(s):  
W. H. Zucker ◽  
R. G. Mason
Keyword(s):  
Fine Structure ◽  
Platelet Aggregation ◽  
Hydrochloric Acid ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Hemostatic Agent ◽  
Native Collagen ◽  
Fibrillar Morphology ◽  
Clinical Interest ◽  
New Form

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.

Electron Microscopic Detection and Characterization of Nonhuman Primate Enteric Viruses

1982 ◽  
Vol 40 ◽  
pp. 306-307
Author(s):  
G. C. Smith ◽  
R. L. Heberling ◽  
S. S. Kalter
Keyword(s):  
Electron Microscopy ◽  
Animal Model ◽  
Nonhuman Primate ◽  
Clinical Condition ◽  
Intestinal Tract ◽  
Enteric Viruses ◽  
Electron Microscopic ◽  
Microscopic Detection

A number of viral agents are recognized as and suspected of causing the clinical condition “gastroenteritis.” In our attempts to establish an animal model for studies of this entity, we have been examining the nonhuman primate to ascertain what viruses may be found in the intestinal tract of “normal” animals as well as animals with diarrhea. Several virus types including coronavirus, adenovirus, herpesvirus, and picornavirus (Table I) were detected in our colony; however, rotavirus, astrovirus, and calicivirus have not yet been observed. Fecal specimens were prepared for electron microscopy by procedures reported previously.

3-D organization of fibrinogen receptors using computer reconstruction and whole-mount microscopy

1988 ◽  
Vol 46 ◽  
pp. 30-31
Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Blood Platelets ◽  
Cell Contact ◽  
Computer Assisted ◽  
Adherent Cells ◽  
Differential Centrifugation ◽  
Computer Reconstruction ◽  
Sds Page ◽  
Whole Mount

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.

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