Novel Curcumae Kwangsiensis mediated synthesis of silver nanoparticles for anti-lung cancer activity: a preclinical trial study

IntroductionThe present work indicated the green synthesis and characterization and cytotoxicity, antioxidant, and anti-human lung cancer activities of silver nanoparticles containing Curcumae Kwangsiensis Folium leaf aqueous extract.Material and methodsAg nanoparticles have been produced by mixing the AgNO3 solution with aqueous Curcumae Kwangsiensis Folium leaf extract. Characterization of Ag nanoparticles was done by FE‐SEM, FT‐IR, TEM, and UV-Vis. FE-SEM and TEM images revealed an average diameter of 15-21 nm for the nanoparticles. MTT assay was used on common human lung cancer cell lines i.e., lung well-differentiated bronchogenic adenocarcinoma (HLC-1), lung moderately differentiated adenocarcinoma (LC-2/ad), and lung poorly differentiated adenocarcinoma (PC-14) cell lines to survey the cytotoxicity and anti-human lung cancer effects of Ag nanoparticles.ResultsThey had very low cell viability and high anti-human lung cancer activities dose-dependently against HLC-1, LC-2/ad, and PC-14 cell lines without any cytotoxicity on the normal cell line (HUVEC). The IC50 of Ag nanoparticles were 249, 187, and 152 µg/mL against HLC-1, LC-2/ad, and PC-14 cell lines, respectively. The best results of cytotoxicity and anti-human lung cancer properties were seen in the concentration of 1000 µg/mL. Ag nanoparticles inhibited half of the DPPH molecules in the concentration of 135 µg/mL.ConclusionsMaybe significant anti-human lung cancer potentials of Ag nanoparticles synthesized by Curcumae Kwangsiensis Folium leaf aqueous extract against common human lung cancer cell lines are linked to their antioxidant activities. After confirming the above results in the clinical trial researches, this formulation can be administrated to treat human lung cancers in humans.