scholarly journals Exhaled breath condensates from healthy children induce cell death of in vitro cultured cells by activation of apoptosis

2019 ◽  
Author(s):  
Alicja Krejner-Bienias ◽  
Katarzyna Grzela ◽  
Wioletta Zagórska ◽  
Magdalena Chojnowska ◽  
Tomasz Grzela
Keyword(s):  
Cell Death ◽  
Cultured Cells ◽  
Exhaled Breath ◽  
Healthy Children ◽  
Induce Cell Death

B cells from patients with multiple sclerosis induce cell death via apoptosis in neurons in vitro

2017 ◽  
Vol 309 ◽  
pp. 88-99 ◽  
Author(s):  
Robert P. Lisak ◽  
Liljana Nedelkoska ◽  
Joyce A. Benjamins ◽  
Dana Schalk ◽  
Beverly Bealmear ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cell Death ◽  
B Cells ◽  
Induce Cell Death

scholarly journals An in vitro study of the cytotoxicity of TTF·TCNQ nanoparticles to mammalian cells

2020 ◽  
Vol 1 (6) ◽  
pp. 1963-1970
Author(s):  
Hongjie Chen ◽  
Géraldine Albérola ◽  
Dominique de Caro ◽  
Christophe Faulmann ◽  
Muriel Golzio ◽  
...  
Keyword(s):  
Cell Death ◽  
Mammalian Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Biomedical Devices ◽  
Induce Cell Death

Soluble functionalized TTF·TCNQ nanoparticles do not induce cell death at concentrations up to 50 μg mL−1, a promising feature for biomedical devices.

Platelet Death.

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. sci-40-sci-40
Author(s):  
Emma C. Josefsson ◽  
Simone Schoenwaelder ◽  
Michael White ◽  
Matthew Goschnick ◽  
Andrew W. Roberts ◽  
...  
Keyword(s):  
Cell Death ◽  
Cultured Cells ◽  
Preliminary Evidence ◽  
Human Platelets ◽  
Mitochondrial Damage ◽  
Intrinsic Apoptosis ◽  
Apoptosis Pathway ◽  
Intrinsic Apoptosis Pathway

Abstract Human platelets exhibit a circulating lifespan of ~10 days, mouse platelets ~5 days. This finite existence is circumscribed by members of the Bcl-2 family of proteins, which control the intrinsic apoptosis pathway. Pro-survival Bcl-xL is the critical regulator of platelet lifespan, functioning to keep pro-death Bak and Bax in check, thereby maintaining platelet viability. After 5–10 days in the circulation, platelets not consumed in hemostatic processes initiate a Bak and Bax-dependent cell death program and clearance from the bloodstream. Mutations in Bcl-xL reduce platelet lifespan in a dose-dependent fashion, while deletion of Bak and Bax extend it. Studies with the BH3 mimetic compound ABT-737, which inhibits pro-survival Bcl-xL, have shown that platelets induced to undergo cell death in vitro exhibit many of the hallmarks of apoptosis in nucleated cells, including mitochondrial damage, caspase activation and externalization of membrane phosphatidylserine (PS). Whether any of these features occur during physiological platelet clearance remains unclear. Certainly, mitochondrial damage can reduce the recovery of transfused platelets, but whether PS – which is known to promote the pro-coagulant activity of agonist-activated platelets – also acts as a clearance signal for dying platelets in vivo is yet to be established. Conversely, Bak and Bax may play a role in mediating PS exposure triggered by activation. Supporting the idea that there may be crosstalk between classical platelet signaling pathways and the intrinsic apoptosis pathway is recent evidence that platelet agonists can also activate caspases. Intriguingly, elements of the intrinsic pathway may also contribute to the generation of platelets by megakaryocytes. Several groups have demonstrated that megakaryocytes contain activated caspases and that their inhibition can block platelet shedding by cultured cells. Preliminary evidence we have generated suggests that Bcl-2 family proteins may be required for platelet production in vivo. Thus, it appears that there is much to be understood about the role of the intrinsic apoptosis pathway in the regulation of platelet biogenesis, function, and death.

scholarly journals Low molecular weight silicones induce cell death in cultured cells

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Carla Onnekink ◽  
Rita M. Kappel ◽  
Wilbert C. Boelens ◽  
Ger J. M. Pruijn
Keyword(s):  
Molecular Weight ◽  
Cell Death ◽  
Cultured Cells ◽  
Low Molecular Weight ◽  
Induce Cell Death

scholarly journals Ablation of RIP3 protects from dopaminergic neurodegeneration in experimental Parkinson’s disease

2019 ◽  
Vol 10 (11) ◽  
Author(s):  
Pedro A. Dionísio ◽  
Sara R. Oliveira ◽  
Maria M. Gaspar ◽  
Maria J. Gama ◽  
Margarida Castro-Caldas ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Cell Death ◽  
Cultured Cells ◽  
Substantia Nigra Pars Compacta ◽  
Neuronal Cultures ◽  
Interacting Protein ◽  
Dopaminergic Neurodegeneration

Abstract Parkinson’s disease (PD) is driven by dopaminergic neurodegeneration in the substantia nigra pars compacta (SN) and striatum. Although apoptosis is considered the main neurodegenerative mechanism, other cell death pathways may be involved. In this regard, necroptosis is a regulated form of cell death dependent on receptor interacting protein 3 (RIP3), a protein also implicated in apoptosis and inflammation independently of its pro-necroptotic activity. Here, we explored the role of RIP3 genetic deletion in in vivo and in vitro PD models. Firstly, wild-type (Wt) and RIP3 knockout (RIP3ko) mice were injected intraperitoneally with MPTP (40 mg/kg, i.p.), and sacrificed after either 6 or 30 days. RIP3ko protected from dopaminergic neurodegeneration in the SN of MPTP-injected mice, but this effect was independent of necroptosis. In keeping with this, necrostatin-1s (10 mg/kg/day, i.p.) did not afford full neuroprotection. Moreover, MPTP led to DNA fragmentation, caspase-3 activation, lipid peroxidation and BAX expression in Wt mice, in the absence of caspase-8 cleavage, suggesting intrinsic apoptosis. This was mimicked in primary cortical neuronal cultures exposed to the active MPTP metabolite. RIP3 deficiency in cultured cells and in mouse brain abrogated all phenotypes. Curiously, astrogliosis was increased in the striatum of MPTP-injected Wt mice and further exacerbated in RIP3ko mice. This was accompanied by absence of microgliosis and reposition of glial cell line-derived neurotrophic factor (GDNF) levels in the striata of MPTP-injected RIP3ko mice when compared to MPTP-injected Wt mice, which in turn showed a massive GDNF decrease. RIP3ko primary mixed glial cultures also presented decreased expression of inflammation-related genes upon inflammatory stimulation. These findings hint at possible undescribed non-necroptotic roles for RIP3 in inflammation and MPTP-driven cell death, which can contribute to PD progression.

scholarly journals THE EFFECT OF SOURSOP (ANNONA MURICATA L.) ESSENTIAL OILS ON VIABILITY CELLS: AN IN-VITRO STUDY

2021 ◽  
Vol 8 (1) ◽  
pp. 28
Author(s):  
Friska Ani Rahman ◽  
Qotru Al Naday ◽  
Trianna Wahyu Utami
Keyword(s):  
Cell Death ◽  
Essential Oils ◽  
Mtt Assay ◽  
Cultured Cells ◽  
In Vitro Study ◽  
Fibroblast Cell ◽  
Purple Colour ◽  
Data Compilation ◽  
Leaf Oil

Background: Development of new preventive agents for dental caries is needed. One of the candidates for preventive agents from natural products is Soursop leaf. The present study aimed to determine the effect of Soursop leaf oil on the cultured epithelial and fibroblast cells.Methods: In this experimental study, Soursop leaf essential oils were provided, and their e?ect was discovered on epithelial and fibroblast cells line using MTT assay. The MTT assay was conducted to measure the activity of enzymes that reduce MTT and switch it to formazan dye creating a purple colour. Using a microplate reader, the optical density was measured at 550 nm and the absorbance value directly represented relative cell numbers.Results: Data compilation and analysis were done using one-way analysis of variance. Soursop leaf essential oils exhibited variable noxious e?ects on cultured cells. The present study shows that epithelial cell death was less than 30% at the concentration 2.5 �l/ml while the percentage of fibroblast cell death was less than 30% at smaller concentrations of 1.25 �l/ml. Through an increase in the concentration of Soursop leaf essential oils, the toxicity of these materials substantially increased (p<0.05)Conclusion: Soursop leaf essential oils at certain concentrations may cause epithelial and fibroblast cell death.

scholarly journals Certain protein transducing agents convert translocated proteins into cell killers.

2012 ◽  
Vol 59 (3) ◽  
Author(s):  
Siergiej Tcherniuk ◽  
Anne-Laure Fiser ◽  
Madiha Derouazi ◽  
Bertrand Toussaint ◽  
Yan Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Cytotoxic Effect ◽  
Cultured Cells ◽  
Large Range ◽  
Protein Transduction ◽  
Cell Interior ◽  
Cell Penetrating ◽  
Translocated Proteins ◽  
Control Protein

The majority of proteins are unable to translocate into the cell interior. Hence for peptide- and protein-based therapeutics a direct intracytoplasmic delivery with the aid of transducing agents is an attractive approach. We wanted to deliver to the cell interior a putatively cytotoxic protein VPg. Protein transduction was achieved in vitro with three different commercial products. However, in our hands, delivery of various control proteins without known deleterious effects, as well as of protein VPg, always induced cell death. Finally, we used a novel transducing peptide Wr-T, which was not toxic to cultured cells, even in a quite large range of concentrations. Most importantly, control protein delivered to cells in culture did not display any toxicity while VPg protein exerted a strong cytotoxic effect. These data show that results obtained with cell-penetrating agents should be interpreted with caution.

scholarly journals Targeted Nanoparticle Photodynamic Diagnosis and Therapy of Colorectal Cancer

2021 ◽  
Vol 22 (18) ◽  
pp. 9779
Author(s):  
Nokuphila Winifred Nompumelelo Simelane ◽  
Cherie Ann Kruger ◽  
Heidi Abrahamse
Keyword(s):  
Colorectal Cancer ◽  
Cell Death ◽  
Cultured Cells ◽  
Zinc Phthalocyanine ◽  
Photodynamic Diagnosis ◽  
Tumor Cell Death ◽  
Aggressive Cancer ◽  
Alternative Techniques ◽  
High Range

Colorectal cancer (CRC) is an aggressive cancer that remains a challenge to diagnose and treat. Photodynamic diagnosis (PDD) and therapy (PDT) are novel alternative techniques, which can enhance early diagnosis, as well as elicit tumor cell death. This is accomplished through photosensitizer (PS) mediated fluorescence and cytotoxic reactive oxygen species activation upon laser light irradiation excitation at specific low and high range wavelengths, respectively. However, the lack of PS target tumor tissue specificity often hampers these techniques. This study successfully fabricated a bioactive nanoconjugate, ZnPcS4-AuNP-S-PEG5000-NH2-Anti-GCC mAb (BNC), based upon a polyethylene glycol-gold nanoparticle, which was multi-functionalized with a fluorescent PDT metalated zinc phthalocyanine PS, and specific anti-GCC targeting antibodies, to overcome CRC PDD and PDT challenges. The BNC was found to be stable and showed selectively improved subcellular accumulation within targeted CRC for improved PDD and PDT outcomes in comparison to healthy in vitro cultured cells. Additionally, the BNC reported significantly higher late apoptotic PDT-induced CRC cell death rates (34% ***) when compared to PDT PS administration alone (15% *). These results indicated that the improved PDD and PDT outcomes were due to the specific PS accumulation in CRC cells through nanoparticle carriage and bioactive anti-GCC targeting.

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