Spectroscopy in biological sciences. IV. Use of synchrotron radiation in biological sciencies.

Kotaro HIEDA

doi:10.5111/bunkou.36.295

Mapping Sub-Cellular Protein Aggregates and Lipid Inclusions Using Synchrotron Radiation ATR-FTIR Microscopy

The Analyst ◽

10.1039/d1an00136a ◽

2021 ◽

Author(s):

David Hartnell ◽

Ashley Hollings ◽

Anna Maria Ranieri ◽

Hum Bahadur Lamichhane ◽

Thomas Becker ◽

...

Keyword(s):

Biological Sciences ◽

Synchrotron Radiation ◽

Biochemical Markers ◽

Protein Aggregates ◽

Cellular Protein ◽

Cellular Level ◽

Cell Physiology ◽

Ftir Microscopy ◽

Lipid Inclusions

Visualising direct biochemical markers of cell physiology and disease pathology at the sub-cellular level is an ongoing challenge in the biological sciences. A suite of microscopies exists to either visualise...

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Dynamical Scattering in Crystalline Biological Specimens. I. Criteria of Validity for Scattering Approximations

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010011458x ◽

1975 ◽

Vol 33 ◽

pp. 192-193

Author(s):

Robert M. Glaeser ◽

Bing K. Jap

Keyword(s):

Biological Sciences ◽

Electron Microscopy ◽

Electron Microscope ◽

Electron Diffraction ◽

Born Approximation ◽

Materials Science ◽

Image Data ◽

Dynamical Effect ◽

Crystallographic Structure ◽

Electron Microscope Image

The dynamical scattering effect, which can be described as the failure of the first Born approximation, is perhaps the most important factor that has prevented the widespread use of electron diffraction intensities for crystallographic structure determination. It would seem to be quite certain that dynamical effects will also interfere with structure analysis based upon electron microscope image data, whenever the dynamical effect seriously perturbs the diffracted wave. While it is normally taken for granted that the dynamical effect must be taken into consideration in materials science applications of electron microscopy, very little attention has been given to this problem in the biological sciences.

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The Application of Ultra-Thin Sectioning Techniques to Non-Biological Samples

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100101402 ◽

1968 ◽

Vol 26 ◽

pp. 332-333

Author(s):

C. F. Oster

Keyword(s):

Biological Sciences ◽

Epoxy Resin ◽

Cross Sections ◽

Biological Samples ◽

Industrial Applications ◽

Photographic Film ◽

Silver Particles ◽

Thin Sectioning ◽

Embedding Medium

Although ultra-thin sectioning techniques are widely used in the biological sciences, their applications are somewhat less popular but very useful in industrial applications. This presentation will review several specific applications where ultra-thin sectioning techniques have proven invaluable.The preparation of samples for sectioning usually involves embedding in an epoxy resin. Araldite 6005 Resin and Hardener are mixed so that the hardness of the embedding medium matches that of the sample to reduce any distortion of the sample during the sectioning process. No dehydration series are needed to prepare our usual samples for embedding, but some types require hardening and staining steps. The embedded samples are sectioned with either a prototype of a Porter-Blum Microtome or an LKB Ultrotome III. Both instruments are equipped with diamond knives.In the study of photographic film, the distribution of the developed silver particles through the layer is important to the image tone and/or scattering power. Also, the morphology of the developed silver is an important factor, and cross sections will show this structure.

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Direct Observation of Monolayer Zones in Al-wt 4% Cu

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100178823 ◽

1990 ◽

Vol 48 (1) ◽

pp. 14-15

Author(s):

B. Jouffrey ◽

D. Dorignac ◽

A. Bourret

Keyword(s):

Solid Solution ◽

Synchrotron Radiation ◽

Direct Observation ◽

Supersaturated Solid Solution ◽

Original Model ◽

X Ray ◽

Image Position

Since the early works on GP zones and the model independently proposed by Preston and Guinier on the first steps of precipitation in supersaturated solid solution of aluminium containing a few percent of copper, many works have been performed to understand the structure of different stages in the sequence of precipitation.The scheme which is generally admitted can be drawn from a work by Phillips.In their original model Guinier and Preston analysed a GP zone as composed of a single (100) copperrich plane surrounded by aluminum atomic planes with a slightly shorter distance from the original plane than in the solid solution.From X-ray measurements it has also been shown that GP1 zones were not only copper monolayer zones. They could be up to a few atomic planes thick. Different models were proposed by Guinier, Gerold, Toman. Using synchrotron radiation, proposals have been recently made.

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